Learn about plasmid quality control standards and testing methods for seed, transfection, and preclinical-grade plasmids, including purity, endotoxin, integrity, and function.
Learn a standard plasmid transformation protocol using competent cells and heat shock methods, including plasmid DNA introduction, bacterial recovery, selection, and colony screening.
Learn the alkaline lysis method for plasmid preparation, including bacterial culture, plasmid extraction, DNA purification, and storage procedures for molecular biology research.
Learn how to prepare and transfect siRNA products with this detailed siRNA protocol, including siRNA handling, cell transfection steps, and experimental controls.
Learn how to use research-use lentivirus for target cell transduction, including 96-well and 6-well plate protocols, MOI optimization, culture conditions, and safety precautions.
Learn the standard lentiviral packaging workflow, including plasmid preparation, 293T cell transfection, virus harvest, titer determination, and key precautions.
