Universal Sequencing Primers for Plasmid Verification
Universal Sequencing Primers for Plasmid Verification
To facilitate plasmid sequencing and sequence verification, MiaoLingPlasmid has compiled a collection of commonly used universal sequencing primers. The list includes primers targeting commonly used plasmid vectors and functional elements, such as T7, SP6, M13, CMV, GFP, pUC, pET, pGEX, pIRES, pEGFP, and pBR322.
| Primer Name | Primer Sequence (5′→3′) |
|---|---|
| pQE-R | GTTCTGAGGTCATTACTGG |
| Psiren-shuttle.F | TCTTGGGTAGTTTGCAGTTT |
| WPRE-R | CATAGCGTAAAAGGAGCAACA |
| Psicheck-2-R | CCCCCGCGAGGTCCGAAGAC |
| T3 | ATTAACCCTCACTAAAGGGA |
| T72G | TAATACGACTCACTATAGG |
| 17base | ACATCCACTTTGCCTTTCTC |
| PVL1393.R | GGGTCCAAGTTTCCCTGTAG |
| SP6 | ATTTAGGTGACACTATAGAA |
| SV40pro-F | TATTTATGCAGAGGCCGAGG |
| PSG5-F | CCATGTTCATGCCTTCTTCT |
| pTriEX-1.R | ATTTGTGAGCCAGGGCATTG |
| U6 | ATGGACTATCATATGCTTACCGTA |
| pTRCHISC.F | AAGGAGGAATAAACCATGGG |
| psiCHECK-2-F | GAAGTTCCCTAACACCGAGT |
| pQE30-F | TGAGCGGATAACAATTTCAC |
| pTRC99C-F | TTGCGCCGACATCATAAC |
| Reverse | gtaacatcagagattttgagacac |
| pRetro-On.F | TTTAGTGAACCGTCAGATCG |
| pTarget.R | GGCTTTACACTTTATGCTTC |
| pSG5-R | GTTTGGACAAACCACAACTA |
| pTRCHISC.R | CTGTATCAGGCTGAAAATCT |
| pTRC99C-R | CTGCGTTCTGATTTAATCTG |
| pShuttle-F | GAAGTGAAATCTGAATAATTTTGT |
| Sv40-polyA-R | GAAATTTGTGATGCTATTGC |
| pTRE2.R | CCCCCTCCATATAACATGAA |
| S1 | CAACGTGAAAAAATTATTATTCGC |
| RVP3 | CTAGCAAAATAGGCTGTCCC |
| Reverse (right ITR) | TACTATGGTTGCTTTGACGT |
| RVP4 | GACGATAGTCATGCCCCGCG |
| pTarget.F | CCAGGATTTTCCCAGTCAC |
| PYES.F | CCTTTTCGGTTAGAGCGGAT |
| pTRE2.F | TCGTTTAGTGAACCGTCAGA |
| pShuttleCMV.R | GTGGTATGGCTGATTATGATCAG |
| S6 | GTAAATGAATTTTCTGTATGAGG |
| pmscv-3 | ACGTGCTACTTCCATTTGTC |
| T7ter | TGCTAGTTATTGCTCAGCGG |
| T7 | TAATACGACTCACTATAGGG |
| PYES2.R | TCGGTTAGAGCGGATGTG |
| S.tag | CGAACGCCAGCACATGGACA |
| pMEP4-F | CTAGCTATAAACACTGCTTG |
| PLCNX-R | ACCTACAGGTGGGGTCTTTCATTCCC |
| PLKO.1-R | CTGCACTGTACCCCCCAATC |
| pGEX-3' | CCGGGAGCTGCATGTGTCAGAGG |
| pEGFP-C-5' | CATGGTCCTGCTGGAGTTCGTG |
| pEGFP-N-5' | TGGGAGGTCTATATAAGCAGAG |
| PFBT.F | TATTCCGGATTATTCATACC |
| PLEXA-N.R | TAAAACCTAAGAGTCACTTT |
| PFAST-F | TATTCCGGATTATTCATACC |
| PGBT9-F | GGCTTCAGTGGAGACTGATA |
| PIRES-F | CTTACTGACATCCACTTTGC |
| PGAP-F | GTCCCTATTTCAATCAATTGAA |
| Petblue-2.f | TGCGGTAGTTTATCACAG |
| PM.F | CATAGAATAAGTGCGACATC |
| Petblue-2.f | GATTCTCCCAGTCACGAC |
| pDSRED-N1-R | TGAAGCGCATGAACTCCTTG |
| pECFP-C-R | TCATTTTATGTTTCAGGTTCAG |
| pIRES2-EGFP.P5' | GTAGGCGTGTACGGTGGGAG |
| pINPOINTPrimer | CGTGACGCGGTGCAGGGCG |
| PLXSN-F | CTTGAACCTCCTCGTTCGAC |
| PGBT9-R | ACAGGAAAGAGTTACTCAAG |
| pIRES2-EGFP.P3' | AACGCACACCGGCCTTATTC |
| PIRES.R | CACTGCATTCTAGTTGTGGT |
| pEGFP-C-3' | TATGGCTGATTATGATCAGT |
| pJET1.2-F | CGACTCACTATAGGGAGAGCGGC |
| pEF-F | TCAAGCCTCAGACAGTGGTTC |
| PM.R | TTTATGTTTCAGGTTCAGGG |
| pGEX-5' | GGGCTGGCAAGCCACGTTTGGTG |
| pJET1.2-R | AAGAACATCGATTTTCCATGGCAG |
| pMEP4-R | GCAGCTTATAATGGTTACAAAT |
| pFLAG-CMV-F | GGTAGGCGTGTACGGTGG |
| pB42ADF | CCAGCCTCTTGCTGAGTGGAGATG |
| pCMV-R | TCCAAACTCATCAATGTATC |
| PBR322.R | GCCTGCCACCATACCCAC |
| pDSRED2-N-R | CGTTCACGGTGCCCTCCATG |
| PDB.F | GAATAAGTGCGACATCATCATC |
| Pcmv5r | ATTATAGAAGGACACCTAGTC |
| PBR322.F | AAGTGCCACCTGACGTCTAA |
| pBI121-R | GTTTTCGCGATCCAGACTGA |
| pBABE-3 | accctaactgacacacattcc |
| pcaggs-R | TATGTCCTTCCGAGTGAGAG |
| pDsRED-express-C1-F | TCCCACAACGAGGACTACAC |
| pCold-R | GGCAGGGATCTTAGATTCTG |
| pB42ADR | AAGCCGACAACCTTGATTGGAG |
| PBAD30.R | CACTTCTGAGTTCGGCATGG |
| PDC316.F | ACGTGGGTATAAGAGGCG |
| pBV220R | CTGCGTTCTGATTTAATCTG |
| pBABE-5 | ctttatccagccctcac |
| pBADfw | CCATAGCATTTTTATCCATAAG |
| pCold-F | ACGCCATATCGCCGAAAGG |
| Pcmv5f | TTCCAAAATGTCGTAATAAC |
| PBAD30.F | AGATTAGCGGATCCTACCTG |
| pBV220F | AAGAAGGGCAGCATTCAAAG |
| PDC316.R | CGATGCTAGACGATCCAG |
| pCMV-F | TCTAAAAGCTGCGGAATTGT |
| pAS2-1.R | AAGAGTTACTCAAGAACAAGAA |
| PDB.R | GTAAATTTCTGGCAAGGTAGAC |
| pcDNA3.1F | CTAGAGAACCCACTGCTTAC |
| pCEP-F | AGAGCTCGTTTAGTGAACCG |
| pcDNA3.1R | TAGAAGGCACAGTCGAGG |
| pcaggs-F | GTTCGGCTTCTGGCGTGT |
| H1 | TCGCTATGTGTTCTGGGAAA |
| HSVtk-pA-R | GTCTCCTTCCGTGTTTCAG |
| NEBF | CCCGCCGCTGCTTTTGCACGTGAG |
| P3' | CTGCAAGGCGATTAAGTTGG |
| P20 | AAGAGTTACTCAAGAACAAGAA |
| GFPfusionREV | CTCCACTGACAGAAAATTTG |
| M13F(-47) | CGCCAGGGTTTTCCCAGTCACGAC |
| hGH-PA-R | CCAGCTTGGTTCCCAATAGA |
| P1 | CCAGGCTTTACACTTTATGC |
| NOS | GATAATCATCGCAAGACCGG |
| GFP-R | CCATCTAATTCAACAAGAATTGGGACAAC |
| M13R | CAGGAAACAGCTATGACC |
| PABAI-R | ATGCTACAAAGGACCTAATG |
| P5' | TGCGTACTGCGGTGATCAAC |
| PAAV-MCS-F | TCATACCTCTTATCTTCCTC |
| P2 | GCGATTAAGTTGGGTAACGC |
| GLP4 | GGGTAGAATGGCGCTGGG |
| P17110 | TACCACTACAATGGATG |
| NEBR? | TATGCTAGTTATTGCTCAG |
| pACT2.R | GTGCACGATGCACAGTTGAA |
| MalE primer | GGTCGTCAGACTGTCGATGAAGCC |
| GLP2 | CTTTATGTTTTTGGCGTCTTCC |
| GFP-F | GGTCCTTCTTGAGTTTGTAAC |
| PAO-AAM.F | GACTGGTTCCAATTGACAAG |
| Forward (left ITR) | CCTCTGACTTGAGCGTCGAT |
| — | GTGCACGATGCACAGTTGAA |
| M13F (M13-20) | TGTAAAACGACGGCCAGT |
| GLP1 | TGTATCTTATGGTACTGTAACTG |
| mU6F | CAGCACAAAAGGAAACTCACC |
| MCHERRY-F | AACGAGGACTACACCATCGT |
| mCherry-R | TTGGTCACCTTCAGCTTGG |
| ITS4 | TCCTCCGCTTATTGATATGC |
| M13R(-48) (RV-M primer) | AGCGGATAACAATTTCACACAGGA |
| P24990 | TCATCGGAAGAGAGTAG |
| ITS1 | TCCGTAGGTGAACCTGCGG |
| P10 | GCCTCTAACATTGAGACAGC |
| 3'BD | TAAGAGTCACTTTAAAATTTGTATAC |
| BAC1 | AACCATCTCGCAAATAAATA |
| Forward | TCGcgttaacgctagcatggatctc |
| BAC2 | ACGCACAGAATCTAGCGCTT |
| 1492R | TACGGCTACCTTGTTACGACTT |
| BGH | TAGAAGGCACAGTCGAGG |
| 35S | GACGCACAATCCCACTATCC |
| DUETUP2 | TTGTACACGGCCGCATAATC |
| 27F | AGAGTTTGATCCTGGCTCAG |
| 3'AD | AGATGGTGCACGATGCACAG |
| CMV-30 | ATAACCCCGCCCCGTTG |
| 3'AOX (pPIC9K-R) | GGCAAATGGCATTCTGACAT |
| CMV-F | CGCAAATGGGCGGTAGGCGTG |
| 3Xflag.R | TAATTTTATTAGGACAAGGCTG |
| EBV-R | GTGGTTTGTCCAAACTCATC |
| CMV-24 | TTAGGACAAGGCTGGTGG |
| 5'AOX (pPIC9K-F) | GACTGGTTCCAATTGACAAGC |
| a-Factor | TACTATTGCCAGCATTGCTGC |
| DUETUP1 | GATCTCGACGCTCTCCCT |
| DuetDOWN1 | GATTATGCGGCCGTGTACAA |
| 3Xflag.F | GCTCTTGTTGGAGCTGCAGT |
Notes
The primer sequences above are listed in the 5′→3′ direction.
Primer names are retained as provided and may correspond to specific plasmid backbones, promoters, tags, or sequencing regions.
For plasmid sequence verification, select a primer based on the vector backbone and the region to be verified, and make sure the expected sequencing read covers the relevant insert or cloning junction.
Before ordering or using a primer, verify the primer sequence against the specific plasmid map or reference sequence being used in your experiment, as similarly named vectors may differ between versions.
Some primer names or sequences in the source list may be specific to particular vector designs. They should therefore be treated as a reference list rather than universally interchangeable sequencing primers.
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