产品声明
For Research Use Only research use only . They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.Plasmid Sequence and Experimental Performance reference/template plasmid . MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.Sequence Verification of Certain Vector Products key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.After-Sales Period for Small-Pack Plasmid Products three months from the date of receipt . Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.Research-Related Risks and Limitation of Liability Citation in Chinese Publications 产品名称（产品货号）由淼灵质粒平台提供。 Citation in English Publications “Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”
质粒序列
一键复制序列（有部分质粒是非全长序列）
GCCCTGCAGGCGGAAGTCAGGTAGACACGACTTCCGCTAGTCCTTGCAAGGTCTGCTGACGTGAGGCGGGGCGGTCGTTTTTGACCGCCCTGCCTTCGTCATGTAGGCTCGCTCGCTGTGCCTGGCGTGTCATCAGACGCCCAGGTCCCGGTGCCGTGAGGCCCGGGCCATCGAGCCGGTGGTACGTGGCTGCGGTCCCCTTGTGAGGGCTGCGCGCCGTGTGCTGTCCGGCGCGCACAGCCTTGAATCCACCCGCGGGGGCCGGCCGGTCTCCGTGAGCTCGAGTAGACGACGGAGACGTAATGGACAAGAAGTACAGCATCGGCCTGGACATCGGCACCAACAGCGTGGGCTGGGCGGTCATCACCGACGAGTACAAGGTCCCCTCCAAGAAGTTCAAGGTCCTGGGCAACACCGACCGGCACTCGATCAAGAAGAACCTGATCGGCGCCCTGCTCTTCGACAGCGGCGAAACCGCCGAGGCGACCCGCCTGAAGCGGACCGCCCGTCGCCGCTACACCCGGCGCAAGAACCGCATCTGCTACCTGCAGGAGATCTTCTCCAACGAGATGGCCAAGGTCGACGACTCGTTCTTCCACCGGCTCGAGGAGAGCTTCCTGGTGGAGGAGGACAAGAAGCACGAGCGCCACCCGATCTTCGGCAACATCGTCGACGAGGTGGCCTACCACGAGAAGTACCCCACCATCTACCACCTCCGCAAGAAGCTGGTCGACTCGACCGACAAGGCGGACCTGCGGCTCATCTACCTGGCCCTCGCGCACATGATCAAGTTCCGCGGCCACTTCCTCATCGAGGGCGACCTGAACCCGGACAACTCCGACGTCGACAAGCTCTTCATCCAGCTGGTGCAGACCTACAACCAGCTGTTCGAGGAGAACCCCATCAACGCCAGCGGCGTCGACGCCAAGGCGATCCTCTCCGCGCGCCTGAGCAAGTCCCGGCGCCTGGAGAACCTCATCGCCCAGCTGCCGGGCGAGAAGAAGAACGGCCTCTTCGGCAACCTGATCGCGCTGTCGCTCGGCCTGACCCCCAACTTCAAGAGCAACTTCGACCTGGCCGAGGACGCGAAGCTCCAGCTGTCCAAGGACACCTACGACGACGACCTGGACAACCTGCTCGCCCAGATCGGCGACCAGTACGCGGACCTCTTCCTGGCCGCGAAGAACCTCTCGGACGCCATCCTGCTCAGCGACATCCTGCGGGTCAACACCGAGATCACCAAGGCCCCGCTGTCGGCGAGCATGATCAAGCGGTACGACGAGCACCACCAGGACCTGACCCTGCTCAAGGCCCTCGTGCGCCAGCAGCTGCCCGAGAAGTACAAGGAGATCTTCTTCGACCAGTCCAAGAACGGCTACGCCGGCTACATCGACGGCGGCGCGTCGCAGGAGGAGTTCTACAAGTTCATCAAGCCGATCCTGGAGAAGATGGACGGCACCGAGGAGCTGCTCGTCAAGCTGAACCGCGAGGACCTGCTCCGCAAGCAGCGGACCTTCGACAACGGCTCCATCCCGCACCAGATCCACCTGGGCGAGCTCCACGCCATCCTCCGGCGCCAGGAGGACTTCTACCCCTTCCTGAAGGACAACCGCGAGAAGATCGAGAAGATCCTGACCTTCCGGATCCCGTACTACGTCGGCCCCCTGGCCCGCGGCAACTCCCGGTTCGCGTGGATGACCCGGAAGTCGGAGGAAACCATCACCCCGTGGAACTTCGAGGAGGTCGTGGACAAGGGCGCCTCCGCGCAGTCGTTCATCGAGCGCATGACCAACTTCGACAAGAACCTCCCGAACGAGAAGGTCCTGCCCAAGCACAGCCTGCTCTACGAGTACTTCACCGTGTACAACGAGCTGACCAAGGTCAAGTACGTGACCGAGGGCATGCGGAAGCCGGCCTTCCTGTCCGGCGAGCAGAAGAAGGCGATCGTCGACCTGCTCTTCAAGACCAACCGCAAGGTCACCGTGAAGCAGCTGAAGGAGGACTACTTCAAGAAGATCGAGTGCTTCGACTCCGTCGAGATCTCGGGCGTGGAGGACCGCTTCAACGCCTCCCTGGGCACCTACCACGACCTGCTCAAGATCATCAAGGACAAGGACTTCCTCGACAACGAGGAGAACGAGGACATCCTGGAGGACATCGTCCTCACCCTGACCCTCTTCGAGGACCGCGAGATGATCGAGGAGCGGCTCAAGACCTACGCCCACCTGTTCGACGACAAGGTGATGAAGCAGCTGAAGCGGCGCCGGTACACCGGCTGGGGCCGCCTCTCCCGGAAGCTGATCAACGGCATCCGGGACAAGCAGAGCGGCAAGACCATCCTGGACTTCCTCAAGTCCGACGGCTTCGCCAACCGCAACTTCATGCAGCTCATCCACGACGACTCGCTGACCTTCAAGGAGGACATCCAGAAGGCCCAGGTGTCCGGCCAGGGCGACAGCCTCCACGAGCACATCGCCAACCTGGCGGGCTCCCCGGCGATCAAGAAGGGCATCCTCCAGACCGTCAAGGTCGTGGACGAGCTGGTCAAGGTGATGGGCCGCCACAAGCCCGAGAACATCGTGATCGAGATGGCCCGGGAGAACCAGACCACCCAGAAGGGCCAGAAGAACTCCCGCGAGCGGATGAAGCGCATCGAGGAGGGCATCAAGGAGCTCGGCTCGCAGATCCTGAAGGAGCACCCGGTCGAGAACACCCAGCTCCAGAACGAGAAGCTGTACCTCTACTACCTGCAGAACGGCCGCGACATGTACGTGGACCAGGAGCTCGACATCAACCGGCTGAGCGACTACGACGTCGACCACATCGTGCCGCAGTCCTTCCTGAAGGACGACTCGATCGACAACAAGGTCCTGACCCGCTCCGACAAGAACCGGGGCAAGTCCGACAACGTGCCCTCGGAGGAGGTCGTGAAGAAGATGAAGAACTACTGGCGCCAGCTGCTCAACGCCAAGCTCATCACCCAGCGCAAGTTCGACAACCTGACCAAGGCCGAGCGGGGCGGCCTGTCGGAGCTCGACAAGGCGGGCTTCATCAAGCGCCAGCTCGTCGAAACCCGGCAGATCACCAAGCACGTGGCCCAGATCCTGGACAGCCGGATGAACACCAAGTACGACGAGAACGACAAGCTGATCCGCGAGGTCAAGGTGATCACCCTCAAGAGCAAGCTGGTGTCCGACTTCCGCAAGGACTTCCAGTTCTACAAGGTCCGGGAGATCAACAACTACCACCACGCCCACGACGCGTACCTGAACGCCGTCGTGGGCACCGCGCTGATCAAGAAGTACCCGAAGCTGGAGTCCGAGTTCGTCTACGGCGACTACAAGGTCTACGACGTGCGCAAGATGATCGCCAAGTCGGAGCAGGAGATCGGCAAGGCCACCGCGAAGTACTTCTTCTACAGCAACATCATGAACTTCTTCAAGACCGAGATCACCCTGGCCAACGGCGAGATCCGCAAGCGGCCCCTGATCGAAACCAACGGCGAAACCGGCGAGATCGTCTGGGACAAGGGCCGCGACTTCGCCACCGTCCGGAAGGTGCTGTCCATGCCGCAGGTCAACATCGTCAAGAAAACCGAGGTGCAGACCGGCGGCTTCAGCAAGGAGTCCATCCTCCCCAAGCGCAACTCGGACAAGCTGATCGCCCGGAAGAAGGACTGGGACCCGAAGAAGTACGGCGGCTTCGACAGCCCCACCGTCGCCTACTCCGTGCTGGTCGTGGCGAAGGTCGAGAAGGGCAAGAGCAAGAAGCTGAAGTCCGTGAAGGAGCTGCTCGGCATCACCATCATGGAGCGCTCCTCGTTCGAGAAGAACCCGATCGACTTCCTGGAGGCCAAGGGCTACAAGGAGGTCAAGAAGGACCTCATCATCAAGCTGCCCAAGTACTCGCTGTTCGAGCTCGAGAACGGCCGCAAGCGGATGCTCGCCAGCGCGGGCGAGCTGCAGAAGGGCAACGAGCTGGCCCTCCCGTCCAAGTACGTCAACTTCCTGTACCTCGCGTCCCACTACGAGAAGCTGAAGGGCTCGCCCGAGGACAACGAGCAGAAGCAGCTCTTCGTGGAGCAGCACAAGCACTACCTGGACGAGATCATCGAGCAGATCTCGGAGTTCAGCAAGCGGGTCATCCTGGCCGACGCGAACCTCGACAAGGTGCTGTCCGCCTACAACAAGCACCGCGACAAGCCGATCCGGGAGCAGGCGGAGAACATCATCCACCTGTTCACCCTCACCAACCTGGGCGCCCCCGCCGCGTTCAAGTACTTCGACACCACCATCGACCGCAAGCGGTACACCAGCACCAAGGAGGTCCTCGACGCGACCCTGATCCACCAGTCCATCACCGGCCTGTACGAAACCCGCATCGACCTCTCCCAGCTCGGCGGCGACTGAGAATTCAGATCTACGCGTTCCCCGCAAAAGCGGCCTTTGACTCCCTGCAAGCCTCAGCGACCGAATATATCGGTTATGCGTGGGCGATGGTTGTTGTCATTGTCGGCGCAACTATCGGTATCAAGCTGTTTAAGAAATTCACCTCGAAAGCAAGCTGATAAACCGATACAATTAAAGGCTCCTTTTGGAGCCTTTTTTTCCGCCCGCGGGACGTGACGAGCGGCACGACTCGACGACTCCGGGCTCCTTTGACGCTGTCCGTCGCGCCGGGTAGCGTAGGACACCGTGCCCGCGCCGTCGGGCCCTCGCGCGTGCACTCGGTCGACCGCTCCCTGCCGGAGTGGGTGCGGGTGCACGGGGTGGCTCCCCACCTCCTCTCGGATCGGTCCTCGCGGACTGCCGCCGTGCGGAGGACCGGGGCGACACGCCCGGGCGCGGGGGTCGGTGCGGGACTCCAGACCTCCGGGGTAGTCGTGCGACGGGCGACGATCCGGGCCGAGCCGGCCGTCCTGGGTGACGGGTGCCGGTCAGACCAGAGAACACCGACAGACGGAGACGTAGGAACCATTCAAAACAGCATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTTACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTAGCTGCTCCTTCGGTCGGACGTGCGTCTACGGGCACCTTACCGCAGCCGTCGGCTGTGCGACACGGACGGATCGGGCGAACTGGCCGATGCTGGGAGAAGCGCGCTGCTGTACGGCGCGCACCGGGTGCGGAGCCCCTCGGCGAGCGGTGTGAAACTTCTGTGAATGGCCTGTTCGGTTGCTTTTTTTATACGGCTGCCAGATAAGGCTTGCAGCATCTGGGCGGCTACCGCTATGATCGGGGCGTTCCTGCAATTCTTAGTGCGAGTATCTGAAAGGGGATACGCGGGTTTTAGAGCTATGCTGTTTTGAATGGTCCCAAAAGTCTTCTCAGCCGCTACAGGGCGCGTCCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAGCGCGCGTAATACGACTCACTATAGGGCGAATTGGGTACCGGGCCCCCCCTCGAGGTCCTCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCACCGGTGAAGACATAGAGCTATGCTGTTTTGAATGGTCCCAAAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGTCTAGAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTGGTTCATGTGCAGCTCCATCAGCAAAAGGGGATGATAAGTTTATCACCACCGACTATTTGCAACAGTGCCGTTGATCGTGCTATGATCGACTGATGTCATCAGCGGTGGAGTGCAATGTCGTGCAATACGAATGGCGAAAAGCCGAGCTCATCGGTCAGCTTCTCAACCTTGGGGTTACCCCCGGCGGTGTGCTGCTGGTCCACAGCTCCTTCCGTAGCGTCCGGCCCCTCGAAGATGGGCCACTTGGACTGATCGAGGCCCTGCGTGCTGCGCTGGGTCCGGGAGGGACGCTCGTCATGCCCTCGTGGTCAGGTCTGGACGACGAGCCGTTCGATCCTGCCACGTCGCCCGTTACACCGGACCTTGGAGTTGTCTCTGACACATTCTGGCGCCTGCCAAATGTAAAGCGCAGCGCCCATCCATTTGCCTTTGCGGCAGCGGGGCCACAGGCAGAGCAGATCATCTCTGATCCATTGCCCCTGCCACCTCACTCGCCTGCAAGCCCGGTCGCCCGTGTCCATGAACTCGATGGGCAGGTACTTCTCCTCGGCGTGGGACACGATGCCAACACGACGCTGCATCTTGCCGAGTTGATGGCAAAGGTTCCCTATGGGGTGCCGAGACACTGCACCATTCTTCAGGATGGCAAGTTGGTACGCGTCGATTATCTCGAGAATGACCACTGCTGTGAGCGCTTTGCCTTGGCGGACAGGTGGCTCAAGGAGAAGAGCCTTCAGAAGGAAGGTCCAGTCGGTCATGCCTTTGCTCGGTTGATCCGCTCCCGCGACATTGTGGCGACAGCCCTGGGTCAACTGGGCCGAGATCCGTTGATCTTCCTGCATCCGCCAGAGGCGGGATGCGAAGAATGCGATGCCGCTCGCCAGTCGATTGGCTGAGCTCATGAGCGGAGAACGAGATGACGTTGGAGGGGCAAGGTCGCGCTGATTGCTGGGGCAACACGTGGAGCGGATCGGGGATTGTCTTTCTTCAGCTCGCTGATGATATGCTGACGCTCAATGCCGTTTGGCCTCCGACTAACGAAAATCCCGCATTTGGACGGCTGATCCGATTGGCACGGCGGACGGCGAATGGCGGAGCAGACGCTCGTCCGGGGGCAATGAGATATGAAAAAGCCTGAACTCACCGCGACGTATCGATGTCGAGGTTCCTCAGGGGAGCCACCCCAGAGAAGCCCTCGGAGCTGAGCGGAGCTATTTCCAAAGCCATGCCAGCTAGAGACAGTGCACACCGCCAAGCATCTGCAAAACCCTCGCCTGGAGGGAAAGTGCAATGTACGTACGCTGGTTTCCCTCCAGAAAGGGATTCTGCGGCTTCTCAACTTTGGAAGAAGAGGCGGAACGAACTGCTGTTTCAGCCTACTCATGTGAGGAGGCTGGTCCTTTACCCTGATGCCCGAGGGCATCAGGGTAAAGGACCAGCCTCGCCAACTAGGAGCGCTACCAAGGCGAACACAACCCAGGACTTGGTAGAACCCTTCGCCGGAGAAGTTCAGACTCATCGGCATGAGCACCCCTCGCCGCGCACTCGGCACCGGTCCGTCGACCACCACCAGGACGTCGTTGTCGACGTCGGCCCCGCGGCTCCTGCCCGCCGAACGCGTCGTCGTCGACGGCCTGGTGCTCATCGACGAGCACCCGGAGCCAGGTGAAAAGCGCCGGCGGACGCTCGGACTGGGCGCGGGATTCCAGCAGTAACCCAGGTCCGCCGGCCACCTCACGGCAGGCAGACCCTCGGCTTTCGCGCCGGGACCGCCATGAGACCGCCACCCGGATGTCCGGGGTGGCGGTCTCATGGCGGTCCCTCAGCGGCCCTACGCGACCGCTGTGTCGACGCGGAGGCAGTCTCCGGGGAGCCGGCCCCAGGGCTGGAGTACCGGGGCGAGCTTGCCCTTGCAGCGGCGGCACACGGGCGACGCGGCGCCGGGCGGGGGAGTCTTGACGTCGACGGTGACCGGCTTCGGCTTCAGGCGCTTCCGAAGGCTGATCGTCGGGCGGATCGCCTCCTTCTTCGGCTGACGCACTCGGTCGAGCGAAGCGGCCAGCTCCTCCGTGCGGGCCTCGTTGGCCTTCCGGCGCGCCTCGCGGAAAGCAGCTTCCTCCTCGGACATGACCTCGAACCTCATCTGGTCAGCGTCAAGGTCGCCCGGCGCGGCCGGCGCTTCCGGGGCGGGCGGGTCCAGGACGTCCTTGCCACACACCAGGCCCCAGGACTCGACGAGCCGCCGGACGCCCGGTAGGCCGTACGTCTCGGCGACCTTGATGAGGTCGAGGCGACGTCCGGCGACGCGGGCGATGTATCGGTACCAGATGTAGGCCGGGATGACCGCGATGGCGACCAGGCCCTCGGTGTCGTCGGTGATCTCCTCCTCGGTGCGGACGTCCTGCTGGATGCCGAGTTCCTTGATCAGCCGGTTCAGGTTCTGCGACCGGTAGTGCTTGCGGACCTGGAACACGCCGAACTCGCGCTCGCGGTACTTCTCGACGAACGGGCCGGGCCGACGAAGCCGCTGCAGCTCGGCGGCCGCCGCGTCGCCCAGGTCGAGCGGTCCCATGCGGTCGTCGCCGCGACCGGCCTTGAAGTTCTGTCCGGCCAGCTCCAGGCCGATCTTGGAGACGCCGCCCTTGGTCTTGTCGCCGTCCTTGTAGAGGTAGCGGGCCTGCTTGCCCGCATCGCCGTCAGCGGCGTCCGCGCCGTTGAGTGGGCGCACGTCGGTGCCGTGGCCCTTGCCCTCACAGGAGCAACCGGGCCGGTCGCACGTCTTGCTGACGGTGTAGCCGCCCGCGGATTCGACCCCGGCGGCCCAGGCTCCGGCGAGTGCGTCGTGGAACGCGGCCTGGGCGTCCGGGCCGAGCACCTCGCGGGTGACCCAGAGCGTGTGCCAGTGCAGGTGCCAGCCGGAGCCCCAGCCGAAGGTGTCCTCGAAGGCCCGCTCGTAGCCGATGATCCCGAAGTCGTCGCGCATCGTGCGCCAGCGGCGGCCGGACGAGCCGTACGCGCCCTTCCAGCCGTCGTGCAAGACCGCGACCAGGCCGTGCCGCATTCCCTTGCGGACGGTGCCGAACGCCATGCGCTCGAAGTGGCGCAACGTGTTCGTGCCAAGGTGCAGCCCGTACCCGGCGTCCGCGAGACCGTCGGCGGCGAGCTGCACGTTCGAGCCCCGTACGGCCAGGATGCGGCTCATGCACCACGGGCAGGTGTGGACGTTGTTGCAGCGGCACGTGTTGCCCCACGTCGCCTCGCCCGGCTTCCACATCAGCTCGGCCGTCCCGGCAGTGAGCCGGGTCCCGCAGCCCTTGAACGCCTCGTTCAGCGACACCGTCTGGTGCCGGTCCCGCCGGGCGAACCGCTCGTCGCGCGGGTCCTCCCGCCCGGCTGTCGCGGCACCCTCGTTTGGGGTAGAACCCGTTCCAGTTACAGCGCTCTGACCTGCAGTGGACGGAGATTTTCCCTTACTACTAAAGCCCGCGTCCGGATTACCCGCTGTAGTCGTGCTTGCTACGCTGCGTGACTGGTCCGCAATGAGACGCTTTGCGCGCTTTCGGCAGGCGTCCGAGCAGTAGATTTTGGGGCGCTTCCCGGGGATGTGGACGATCGGGGTGCCGCAGTGGCACTTCGGTCCGGCGGGGCGCGGTGGTGTCGACGCGCTGTTCTCTCGTACGCTCGTCACAGAGCAAACGTCCTCACTCGGCATGCTGCGCCAGTTGCGGGGGCGGCGAGCCCGGGAGGCCAATCCCGGGCTCGTGCCATTTCTGGGTCCTGTTGATCCTGGCATTGGTGTGGCCGTTCATTGCCCCTGCTCGCTCCTGACGCGCCGATAGACGTCCGATACGCCCGGTGCTGGTGGGATTTGATAGGTCGGAAGAAGCCCCGCCCGGGCCTGGGCGGGGCTTCCTGTGCGTCAGGACCTCCTCGTCGTGAGCCTCTTCGGCCTATGGACGGAGTGACCTCGTGATCCGTTACAGCCGCGCGCGCTCGCGTAGAGCGGTCTCATCAGTTCCACGAACGGTCCTCTTCGCAGATCAGGGCGTTGGGGCGGAGTCTCACCAAGGACTACGTCTGCTGGCGATTTCCGTTACACCCCGGGCGGTGGCCGGCGCACACGCGCGCCCGCGTTGGGCAGTGCAGAAAGTGCAGAAACCTAGGCGCTGATGGTCCAGGTCCACGGTTCGTCGTCGGCGGCGGCGCGGGCGGCGGCGTCGGCCAGGGCGCGGGCGAGACCGGCTACGGCGGGCTTGATGCGCCGGTTGCGGGCGACCTTGAGCAGCTAGTATGCAGGTCGACGGATCTTTTCCGCTGCATAACCCTGCTTCGGGGTCATTATAGCGATTTTTTCGGTATATCCATCCTTTTTCGCACGATATACAGGATTTTGCCAAAGGGTTCGTGTAGACTTTCCTTGGTGTATCCAACGGCGTCAGCCGGGCAGGATAGGTGAAGTAGGCCCACCCGCGAGCGGGTGTTCCTTCTTCACTGTCCCTTATTCGCACCTGGCGGTGCTCAACGGGAATCCTGCTCTGCGAGGCTGGCCGGCTACCGCCGGCGTAACAGATGAGGGCAAGCGGATGGCTGATGAAACCAAGCCAACCAGGAAGGGCAGCCCACCTATCAAGGTGTACTGCCTTCCAGACGAACGAAGAGCGATTGAGGAAAAGGCGGCGGCGGCCGGCATGAGCCTGTCGGCCTACCTGCTGGCCGTCGGCCAGGGCTACAAAATCACGGGCGTCGTGGACTATGAGCACGTCCGCGAGCTG
质粒元件
rpsL promoter 1..302
/note="纤维素木霉核糖体蛋白S12基因启动子"
CDS 303..4409
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/note="在DNA中产生RNA引导的双链断裂"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
rpsL promoter 4609..4969
/note="黄单胞菌核糖体蛋白S12基因启动子"
misc_RNA 4970..5048
/note="化脓性链球菌CRISPR/Cas9系统的反式激活CRISPR RNA"
terminator 5072..5105
/note="λ噬菌体的最小转录终止子（Scholtissek和Grosse，1987）"
gapdh promoter 5106..5390
/note="茄子甘油醛-3-磷酸脱氢酶启动子"
lacZ-alpha complement(5434..5730)
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSG"
primer_bind 5595..5611
/note="常见的测序引物，多种相似变体之一"
T7 promoter 5621..5639
/note="噬菌体T7 RNA聚合酶启动子"
T3 promoter complement(5687..5705)
/note="噬菌体T3 RNA聚合酶启动子"
primer_bind complement(5726..5742)
/note="常见的测序引物，多种相似变体之一"
lac operator 5750..5766
/bound_moiety="lac repressor encoded by lacI"
/note="lac阻遏物与lac操作员结合，抑制大肠杆菌中的转录。这种抑制可以通过添加乳糖或异丙基β-D-硫代吡喃半乳糖苷（IPTG）来缓解。"
lac promoter complement(5774..5804)
/note="大肠杆菌lac操纵子的启动子"
/note="此反向特征有3个分段：1:5774。.5780/#fffff/-10 2:5781。.5798/#fffff 3:5799。.5804/#fffff/-35"
CAP binding site 5819..5840
/bound_moiety="E. coli catabolite activator protein"
/note="CAP结合在cAMP存在下激活转录。"
terminator 5913..5960
/note="噬菌体T7 RNA聚合酶的转录终止子"
ori complement(6003..6591)
/direction=LEFT
/note="高拷贝数ColE1/pMB1/pBR322/pUC复制起点"
ApmR 6795..7595
/codon_start=1
/gene="aac(3)IV"
/product="aminoglycoside 3-N-acetyltransferase type IV
"
/note="赋予对阿普霉素的抗性（Paget和Davies，1996）"
/translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
CDS complement(8534..9976)
/codon_start=1
/gene="rep"
/product="replication initiator protein from the
Streptomyces ghanaensis plasmid pSG5"
/translation="MWKPGEATWGNTCRCNNVHTCPWCMSRILAVRGSNVQLAADGLAD
AGYGLHLGTNTLRHFERMAFGTVRKGMRHGLVAVLHDGWKGAYGSSGRRWRTMRDDFGI
IGYERAFEDTFGWGSGWHLHWHTLWVTREVLGPDAQAAFHDALAGAWAAGVESAGGYTV
SKTCDRPGCSCEGKGHGTDVRPLNGADAADGDAGKQARYLYKDGDKTKGGVSKIGLELA
GQNFKAGRGDDRMGPLDLGDAAAAELQRLRRPGPFVEKYREREFGVFQVRKHYRSQNLN
RLIKELGIQQDVRTEEEITDDTEGLVAIAVIPAYIWYRYIARVAGRRLDLIKVAETYGL
PGVRRLVESWGLVCGKDVLDPPAPEAPAAPGDLDADQMRFEVMSEEEAAFREARRKANE
ARTEELAASLDRVRQPKKEAIRPTISLRKRLKPKPVTVDVKTPPPGAASPVCRRCKGKL
APVLQPWGRLPGDCLRVDTAVA"
oriT 11144..11253
/note="incP转让来源"
