| Plasmid Name | pET His6 Sumo TEV LIC cloning vector (2S-T) | Alternative Names | 等效质粒Plasmid#29711 |
|---|---|---|---|
| Promoter | T7 | Replicon | pBR322 |
| Copy Number | high | Competent Cell | DH5a |
| Culture Temp | 37°C | Forward Primer | T7：TAATACGACTCACTATAGGG |
| Reverse Primer | T7ter：TGCTAGTTATTGCTCAGCGG | Storage Temp | 37°C |
1.For Research Use Only
All products available through the MiaoLing Plasmid Platform are intended for research use only. They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.
2.Plasmid Sequence and Experimental Performance
Plasmid products are provided and sold based on the corresponding reference/template plasmid. MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.
3.Sequence Verification of Certain Vector Products
For certain vector products, even the original manufacturer may not have fully verified the complete plasmid sequence. If the sequence of the key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.
4.After-Sales Period for Small-Pack Plasmid Products
The after-sales service period for small-pack plasmid products is three months from the date of receipt. Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.
5.Research-Related Risks and Limitation of Liability
Scientific research is inherently exploratory and involves a significant degree of uncertainty. To the maximum extent permitted by applicable law, MiaoLing shall not be liable for any additional economic losses, indirect losses, or other liabilities arising from the use of our products or from research outcomes.
6.Citation in Chinese Publications
For publications in Chinese, please cite the product as follows:
7.Product Name (Product Item Number) was provided by the MiaoLing Plasmid Platform.
Citation in English Publications
For publications in English, please cite the product as follows:
“Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”
T7 promoter 1..19
/note="噬菌体T7 RNA聚合酶启动子"
RBS 108..130
/note="噬菌体T7基因10的高效核糖体结合位点（Olins和Rangwala，1989）"
6xHis 150..167
/codon_start=1
/product="6xHis affinity tag"
/translation="HHHHHH"
SUMO 186..476
/codon_start=1
/gene="S. cerevisiae SMT3 (truncated)"
/product="cleavable ubiquitin-like protein tag"
/translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIG"
CDS 486..506
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
Cleavage site after base 503"
/translation="ENLYFQS"
terminator 645..692
/note="噬菌体T7 RNA聚合酶的转录终止子"
tet promoter complement(1058..1086)
/note="四环素外排蛋白基因大肠杆菌启动子"
/note="此反向特征有3个分段：1:1058。.1063/#fffff/-10 2:1064。.1080/#fffff 3:1081。.1086/#fffff/-35"
AmpR promoter 1199..1303
/gene="bla"
AmpR 1304..2164
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="对氨苄青霉素、羧苄青霉素和相关抗生素产生耐药性"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
ori 2335..2923
/direction=RIGHT
/note="高拷贝数ColE1/pMB1/pBR322/pUC复制起点"
bom 3109..3248
/note="pBR322迁移区的基础"
rop complement(3350..3541)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL" 