pRH2521

Catalog Number P1207

Alternative Names 等效质粒Plasmid#84380

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Custom Scale / Special Requirements / Custom ProjectsPreclinical-Grade PlasmidSmall-Scale Dry Plasmid (Requires Transformation & Preparation)Transfection-Grade Plasmid, 100 μg (Ready-to-Use, Liquid)Transfection-Grade Plasmid, 200 μg (Ready-to-Use, Liquid)Transfection-Grade Plasmid, 500 μg (Ready-to-Use, Liquid)

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Transfection-Grade Plasmid QC Standards View Plasmid Manual

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Plasmid Elements

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Product Overview

Product Disclaimer

For Research Use Only
All products available through the MiaoLing Plasmid Platform are intended for research use only. They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.
Plasmid Sequence and Experimental Performance
Plasmid products are provided and sold based on the corresponding reference/template plasmid. MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.
Sequence Verification of Certain Vector Products
For certain vector products, even the original manufacturer may not have fully verified the complete plasmid sequence. If the sequence of the key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.
After-Sales Period for Small-Pack Plasmid Products
The after-sales service period for small-pack plasmid products is three months from the date of receipt. Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.
Research-Related Risks and Limitation of Liability
Scientific research is inherently exploratory and involves a significant degree of uncertainty. To the maximum extent permitted by applicable law, MiaoLing shall not be liable for any additional economic losses, indirect losses, or other liabilities arising from the use of our products or from research outcomes.
Citation in Chinese Publications
For publications in Chinese, please cite the product as follows:
产品名称（产品货号）由淼灵质粒平台提供。
Citation in English Publications
For publications in English, please cite the product as follows:
“Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”

质粒序列

AACGTTCTCGGCTCGATGATCCCCGGGGGCCCCCCCTCGAGGTCGACGGTATCGATAATATTGGATCGTCGCACCGGGTTAAGCCTGGAGTGCGGTGGTGCCTGGTCGGCATTTTCGCAGTCGAGGGCTCTCGTGTAGCCTGGGCGAGTTGCCGACGCAGGCGACCCTCCTGCCACGGATCCGTGATTAGCTAAGCAGAAGGCTATCCTGACGGATGGCCTTTTTGCGTTTAATACTGTTTAAACTCTAGAAATATTGGATCGTCGGCACCGTCACGGCCGTGGGAGGCGGCACGATCCGCGACGTGATGATCGGCCGCATCCCCACGGTGCTGCGCAGTGAGCTCTACGCCATCCCGGCGTTGATCTGTGCGTTCGCACGCACAGGCCCGGTGTGAGAAGGGTCTCTGACGAGCGGGAGAACTCCCTATCAGTGATAGAGTTTGTCCTCCCTATCAGTGATAGATAGGCTATGGGAGTGTCTTCGAGACAGAGGAAGACATGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTATCGATTTAATTAATATGCATCAATTGATTTAAATACTAGTGTTAACTATTTAATTGGGGACCCTAGAGGTCCCCTTTTTTATTTTAAAAATTTTTTCAAGGGGACCTCTAGGGTCCCCAGCTGGCTAGCGAGTCATGAGGTCACCATGGTCATTAGGAGCCGCTCTCGCACTTCAGCTGCTTCTCCAAGCCGCAGATGATCAGCTCCAGGCCGAACAAGAAGGCCGGCTCGGCGCCCTGGTGGTCGAACAGCTCGATGGCCTGGCGCAGCAACGGCGGCATGCTGTCGGTGGTCGGGGTCTCGCGCTCCTCCTTGGCGACCTGGTGCTCCTGGTCCTCCAACACGCAGCCCAGGGTGAAGTGGCCCACGGCCGACAGGGCGTACAACGCGTTCTCCAGCGAGAAGCCCTGCTGGCACAGGAAGGCCAACTGGTTCTCCAGGGTCTCGTACTGCTTCTCGGTCGGCCGGGTGCCCAGGTGCACCTTGGCGCCGTCGCGGTGGCTCAGCAAGGCGCAGCGGAAGCTCTTGGCGTTGTTGCGCAGGAAGTCCTGCCAGCTCTCGCCCTCCAGCGGGCAGAAGTGGGTGTGGTGGCGGTCCAACATCTCGATGGCCAGGGCGTCGAGCAGGGCCCGCTTGTTCTTGACGTGCCAGTACAGGGTCGGCTGCTCGACGCCCAGCTTCTGGGCCAGCTTGCGGGTGGTCAGGCCCTCGATGCCGACCTCGTTCAGCAGCTCCAGGGCGGAGTTGATGACCTTGGACTTGTCCAGGCGGGACATTGCGAAGTGATTCCTCCTGATGGTACCGGATCCGTGGCAGGAGGGTCGCCTGCGTCGGCAACTCGCCCAGGCTACACGAGAGCCCTCGACTGCGAAAATGCCGACCAGGCACCACCGCACTCCAGGCTTAACCCGGTGCGACGATCCAATATTACGAGATCTGATATCCTCGAGGCATGCCACGTGCCTGCAGGTTTAAACGCGGCCGCAAACAGTATTAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGCTAATCAACTAGTTCTAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGACGTGGCCGACCAGCCCGTCATCGTCAACGCCTGATCCGCGGTGCGGACAGGCCGTGTCGCGACCGGCCGTGCGGAATTAAGCCGGCCCGTACCCTGTGAATAGAGGTCCGCTGTGACACAAGAATCCCTGTTACTTCTCGACCGTATTGATTCGGATGATTCCTACGCGAGCCTGCGGAACGACCAGGAATTCTGGGAGCCGCTGGCCCGCCGAGCCCTGGAGGAGCTCGGGCTGCCGGTGCCGCCGGTGCTGCGGGTGCCCGGCGAGAGCACCAACCCCGTACTGGTCGGCGAGCCCGGCCCGGTGATCAAGCTGTTCGGCGAGCACTGGTGCGGTCCGGAGAGCCTCGCGTCGGAGTCGGAGGCGTACGCGGTCCTGGCGGACGCCCCGGTGCCGGTGCCCCGCCTCCTCGGCCGCGGCGAGCTGCGGCCCGGCACCGGAGCCTGGCCGTGGCCCTACCTGGTGATGAGCCGGATGACCGGCACCACCTGGCGGTCCGCGATGGACGGCACGACCGACCGGAACGCGCTGCTCGCCCTGGCCCGCGAACTCGGCCGGGTGCTCGGCCGGCTGCACAGGGTGCCGCTGACCGGGAACACCGTGCTCACCACCCATTCCGAGATCTTCCCGGAACTGCTGCGGGAACGCCGCGCGGCGACCGTCGAGGACCACCGCGGGTGGGGCTACCTCTCGCCCCGGCTGCTGGACCGCCTGGAGGACTGGCTGCCGGACGTGGACACGCTGCTGGCCGGCCGCGAACCCCGGTTCGTCCACGGCGACCTGCACGGGACCAACATCTTCGTGGACCTGGCCGCGACCGAGGTCACCGGGATCGTCGACTTCACCGACGTCTATGCGGGAGACTCCCGCTACAGCCTGGTGCAACTGCATCTCAACGCCTTCCGGGGCGACCGCGAGATCCTGGCCGCGCTGCTCGACGGGGCGCAGTGGAAGCGGACCGAGGACTTCGCCCGCGAACTGCTCGCCTTCACCTTCCTGCACGACTTCGAGGTGTTCGAGGAGACCCCGCTGGATCTCTCCGGCTTCACCGATCCGGAGGAACTGGCGCAGTTCCTCTGGGGGCCGCCGGACACCGCCCCCGGCGCCTGACGCCCCGGGCCGCCCGGCGCCGCCCCCGGCCCCCGGCGGCCGGCCGCCCGGAGCCCCGCCCGCGCTCGGGAGCCCCGGGCCCGCGCCGAAGCCCGCTGCTGCGAGCCCGGAGCGGGCCGGCCGACGGCGGTGCGGGCCCGGCGGCGGACGCTCAGCAGCGGCGGGCGTGAAAGGCCCTGGCATCCTCGATCATCTCCTCCAGGGTGGTCGGCCGAGCTTCCATCCCAGCTCGGCAAGGATCAACGAGGACAGTCGCACGACGAAGTTCTTCTGGATCGCGCCCGTGCTGGAAGCACTCAACCTCGAAGCGTGTGGTTGCGGAGCCATCTAGCAACCACACGAAACATGCGCAACGAACCGCGCAACGAACAACGCCTAGAACTGGCCCTAGATGAGCTGACTCGTATCGTTGGTAAACCTAGTTTGACCAGCATGTTTTAACTACGTTCGGTGAGCTGTCAACGGGGCCTGTAACGGCACAACGAACCGTGCAACGAGAGTGGCCACGGATGCCACCACAGGCACTACAACGGAGTTCGCCACGTACATCACCACAACCACCGATTCTGGCGGTGAGCTCCCCGATATTCAGCGGAAATGGCTTGGTATCGACCAAGATTCGTAGAACCCCGTCTCGTCTGGCTGGTATTCAAAACGGGCGCAACGAAACACGCAACGAGACAGGCATGGCCCAAACCAGAAAACTAGCGTCTACCAGGACTTTTACCTGTCCGACCCGTTGCAACGGAACCCCCCACGGAACCCCCGCGACACCCGCTCCCCAATTGCGTTAGAACAGCGGTGGATTGTCGGCTTCGTTGTGGGCCTTTTGAGCCGCTTCCTGTTCTGCCGCACGCTCTTTCCTCGCCCGATAGCCGAGTCGCTTAACGGTGTCCAGATGCAGCCCGAAATGTTTGGCCGTTTGCGGCCAAGAGTGGCCCTCGTCGTCGTGATAGGCGCGGATGCGTTCGCGGCGTGCAGCCTGCTCGGCGAGCCACTCGCTGCGTTCCTGCGCCACGAGCCGGACGACGTGGCGTTCGGATAGTCCGGTGATTCGAGCGCCTTCGGCGGCGGTCACGCGCCGCTTTTTGCGGACAGTCGGCTGCCGGTTGTAGCCGTCGCTGTAGCCGTCGCTCATAGCAATGCCTCCATGGCTGACGCGGACTTTGCGCGCCGCGCAACTGTGCTCGCCGCCGTGCGCGCTGCTGCGCCCTTCCGCGAGATGGCCGACTGGCGCGCACTGAGTGTGGCCTCGTAGACCACGATCCCGTCCGCCCAAATGCGCGACTTGGTTGTGATCCAACGCCAAATGCTGTTGGCGATGGCGCGGACCTCGCTGTCCGGTAGCGGTCCGGGACACACGTCGTTGCACGGGAATTCGGCGTTTCGCGCGTGGCACTCGGCATAGATCGCGCGGCCGAGTCCGTCCACGTTCCGGGTCGGCAGGTAGATCCGCATGAGGGCGGGACGATAGGCCCACAACCTGACGGAATCGAACAGTGCGCAATTCCGCCCTAGCGGCGTCGGAGCCGCTTTGTACGTGGTCTGCTGACGCCAGCGCGGCGGTGGCATGTTCGCGCCGAGCTCGGCCTCGATGTGGCTGAGTGTGTAGAGATCTGAGTGGAGCCATTCCGTTTCCCAGGCGATGTGGCCGGGGTTTTTGGTCATGAGGCCTGAGTAACTGCGGTCGCCGTCGACGGCGCGCCGAAGGCCTTCGGCGCACGCCGCCATGTATGCGAGCGGCTTACGCCGCGCGTATTCGGTGCGTGGAACAGGGGCGTTGAGTGCCCACACTGCGTGTGCGTGGCCGTTGGCGCGATTGCCCACGATCGCGTTGGGCAGCGGATGGGACCCCCGGGCGCTGAGCGCTCGGAGCGCTGCGTCTGGATGGTCTACGTCCACGACCAGCAGGTTTGCCAGCGCTGTTGGGTTCGCCTCGATGTACCGGCGGCCTAGGGCCGACGCGCGGCTTTGGCGGTAGATCCCCTCGAGCAGATCGTCGCTTGCCAGCGGCCAGTACGGCAGCCAGAGCTGCTCAAATTCGTCGGCGACGTGGCTCACGCTTGGTAGTAGACCACGATTAATCACCGGTGTATGGTCCGACACGAGCTCCAAGTCAGATATTTCGCTGAGGGGCCACCCCACAACTGCACACTCCCCCGCTCTCCCGTCGAGCCCTGGTGGTGGAACACCAGCGACAGCCGAGCACCCCCAACCACCTGTACCAACCAGGAGGAACACATGCGTCGTTTCGAGGACGTTTCCGGGCCGCTGAGAGCCGCTGTGGCGGCCGTACACGCCGCCTTAGACCCGTTAGACCCCCTGCCGCCTGAATGCGCGGGTACGAGCCACACAGCGCCCGAACTTACGGAGCTGGTGGGCTCACCTGGCTTTATGGCGTACGAATCGGCTGTGTGCGACCTGTTGGGCGAGGTGAGGTACGCGCTACTCACGCTGGCAAGGGCGACAGAGCCGCCCCACCGAGCCCGCACGGCCGCGCGCGGTGTCAACAACCGGGTGAGTCGTGCACACCAGCAGGTGTTCGAGGCTTGGCTCGAAGTGCAGGACATCGTGGCGAACGCCGCCCGATGAGCCGCGCCTTACGCTGGCTGCCAGCCGTTCGCGGGCTGGTTGGTGCAGCGCGTCGAGCGGTTAGAGGCCCTGCGGTGTTCCACCACCGCAGGGCCTCGCCCTTTTTAAGGCTGAATTTGCTTGTCTCCGAATCCAACTGGCTTGTCCAAGGGTGTATCTACGCTTAATCCAAAGTTCAAACGAGGGGATTACACATGACCAACTTCGAT

Related Publications

1、 Hongjuan Zhang; Ying Chen; Yu Zhang; Luyao Qiao; Xiangyin Chi; Yanxing Han; Yuan Lin; Shuyi Si; Jiandong Jiang,Identification of anti-Mycobacterium tuberculosis agents targeting the interaction of bacterial division proteins FtsZ and SepFe,2023-2-4,DOI:10.1016/j.apsb.2023.01.022,(IF:14.8)

Product Documents

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Plasmid Elements

                                                            
     Pimyc promoter    63..182
     terminator      197..224
                     /note="大肠杆菌rrnB基因的转录终止子T2"
     Pmyc1 promoter    244..423
     primer_bind     361..380
     tet operator    424..441
                     /gene="tetO"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="tetR和tetA基因的细菌操作员O2"
     misc_RNA        503..578
                     /note="化脓性链球菌CRISPR/Cas9系统的RNA支架"
     TetR             complement(738..1361)
                     /codon_start=1
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     Pimyc promoter    complement(1389..1508)
     terminator      1588..1615
                     /note="大肠杆菌rrnB基因的转录终止子T2"
     ori      complement(1876..2464)
                     /direction=LEFT
                     /note="高拷贝数ColE1/pMB1/pBR322/pUC复制起点"
     Hyg             complement(2843..4006)
                     /codon_start=1
                     /translation="MPGPFTPAAAERPPPGPHRRRPARSGLAAAGFGAGPGLPSAGGAP
                     GGRPPGAGGGAGRPGASGAGGGVRRPPEELRQFLRIGEAGEIQRGLLEHLEVVQEGEGE
                     QFAGEVLGPLPLRPVEQRGQDLAVAPEGVEMQLHQAVAGVSRIDVGEVDDPGDLGRGQV
                     HEDVGPVQVAVDEPGFAAGQQRVHVRQPVLQAVQQPGREVAPPAVVLDGRRAAFPQQFR
                     EDLGMGGEHGVPGQRHPVQPAEHPAEFAGQGEQRVPVGRAVHRGPPGGAGHPAHHQVGP
                     RPGSGAGPQLAAAEEAGHRHRGVRQDRVRLRLRREALRTAPVLAEQLDHRAGLADQYGV
                     GALAGHPQHRRHRQPELLQGSAGQRLPEFLVVPQARVGIIRINTVEK"
     oriM    complement(4090..6616)                             
                        