For Research Use Only All products available through the MiaoLing Plasmid Platform are intended for research use only. They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.
Plasmid Sequence and Experimental Performance Plasmid products are provided and sold based on the corresponding reference/template plasmid. MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.
Sequence Verification of Certain Vector Products For certain vector products, even the original manufacturer may not have fully verified the complete plasmid sequence. If the sequence of the key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.
After-Sales Period for Small-Pack Plasmid Products The after-sales service period for small-pack plasmid products is three months from the date of receipt. Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.
Research-Related Risks and Limitation of Liability Scientific research is inherently exploratory and involves a significant degree of uncertainty. To the maximum extent permitted by applicable law, MiaoLing shall not be liable for any additional economic losses, indirect losses, or other liabilities arising from the use of our products or from research outcomes.
Citation in Chinese Publications For publications in Chinese, please cite the product as follows: 产品名称（产品货号）由淼灵质粒平台提供。
Citation in English Publications For publications in English, please cite the product as follows: “Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”
CMV enhancer 4..383
/note="人类巨细胞病毒即刻早期增强子"
chicken beta-actin promoter 385..661
intron 662..1678
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 1602..1624
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 1686..1705
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
F2A 2718..2783
/codon_start=1
/product="2A peptide from foot-and-mouth disease virus
polyprotein"
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
Cleavage site after base 2780"
/translation="VKQTLNFDLLKLAGDVESNPGP"
WPRE 4271..4859
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4324..4344)
/note="WPRE, reverse primer"
Factor Xa site complement(4742..4753)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
Cleavage site after base 4741"
/translation="IEGR"
primer_bind complement(4949..4968)
/note="Rabbit beta-globin polyA region, reverse primer"
beta-globin poly(A) signal 5014..5069
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(5068..5087)
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(5430..5446)
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5430..5446)
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5443..5465)
/note="In lacZ gene"
lac operator 5454..5470
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
lac promoter complement(5478..5508)
/note="promoter for the E. coli lac operon"
CAP binding site 5523..5544
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
loxP complement(5633..5666)
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
EBNA1 6127..8052
/codon_start=1
/product="Epstein-Barr nuclear antigen 1, also known as
EBNA-1"
/note="crucial for latent viral infection, and for episomal
amplification of vectors containing the oriP origin (Okita
et al., 2013)"
/translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
GEEGQE"
loxP complement(8220..8253)
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
oriP 8440..10229
/note="Epstein-Barr virus oriP replication origin (Yates et
al., 2000)"
primer_bind complement(10771..10788)
/note="L4440 vector, forward primer"
ori complement(10942..11530)
/direction=LEFT
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(11022..11041)
/note="pBR322 origin, forward primer"
AmpR complement(11701..12561)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
Cleavage site after base 12492"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 12324..12343
/note="Ampicillin resistance gene, reverse primer"
AmpR promoter complement(12562..12666)
/gene="bla"
