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pCXLE-hUL

产品货号 P0500
产品别称 等效质粒Plasmid#27080;LIN28A;CSDD1;LIN28;LIN-28;ZCCHC1;lin-28A
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价格： ¥580.00
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基本信息

产品简介

产品声明

  1. For Research Use Only
    All products available through the MiaoLing Plasmid Platform are intended for research use only. They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.
  2. Plasmid Sequence and Experimental Performance
    Plasmid products are provided and sold based on the corresponding reference/template plasmid. MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.
  3. Sequence Verification of Certain Vector Products
    For certain vector products, even the original manufacturer may not have fully verified the complete plasmid sequence. If the sequence of the key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.
  4. After-Sales Period for Small-Pack Plasmid Products
    The after-sales service period for small-pack plasmid products is three months from the date of receipt. Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.
  5. Research-Related Risks and Limitation of Liability
    Scientific research is inherently exploratory and involves a significant degree of uncertainty. To the maximum extent permitted by applicable law, MiaoLing shall not be liable for any additional economic losses, indirect losses, or other liabilities arising from the use of our products or from research outcomes.
  6. Citation in Chinese Publications
    For publications in Chinese, please cite the product as follows:
    产品名称（产品货号）由淼灵质粒平台提供。
  7. Citation in English Publications
    For publications in English, please cite the product as follows:
    “Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”

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质粒元件

CMV enhancer 4..383 /note="人类巨细胞病毒即刻早期增强子" chicken beta-actin promoter 385..662 intron 663..1679 /note="鸡β-肌动蛋白和兔β-球蛋白内含子之间的嵌合体" primer_bind 1603..1625 /note="嵌合内含子在CAG启动子中，正向引物" primer_bind 1687..1706 /note="兔β-珠蛋白内含子，用于pCAG质粒，正向引物" F2A 2860..2925 /codon_start=1 /product="2A peptide from foot-and-mouth disease virus polyprotein" /note="真核核糖体无法在甘氨酸和脯氨酸残基之间插入肽键，产生分离的多肽。" Cleavage site after base 2922" /translation="VKQTLNFDLLKLAGDVESNPGP" WPRE 3630..4218 /note="土拨鼠肝炎病毒转录后调控元件" primer_bind complement(3683..3703) /note="WPRE，反向引物" Factor Xa site complement(4101..4112) /codon_start=1 /product="Factor Xa recognition and cleavage site" Cleavage site after base 4100" /translation="IEGR" primer_bind complement(4309..4328) /note="兔β-珠蛋白polyA区域，反向引物" beta-globin poly(A) signal 4374..4429 /note="兔β-珠蛋白多聚腺苷酸信号（Gil和Proudfoot，1987）" primer_bind complement(4428..4447) /note="兔β-珠蛋白polyA，反向引物。亦称为rb-glob-pA-term-R。" primer_bind complement(4790..4806) /note="通用测序引物，多个类似变体之一" primer_bind complement(4790..4806) /note="在lacZ基因中。也称为M13-rev。" primer_bind complement(4803..4825) /note="In lacZ gene" lac operator 4814..4830 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." lac promoter complement(4838..4868) /note="大肠杆菌乳糖操纵子的启动子" CAP binding site 4883..4904 /bound_moiety="E. coli catabolite activator protein" /note="CAP结合激活了cAMP存在的条件下的转录。" loxP complement(4993..5026) /bound_moiety="Cre recombinase" /note="Cre介导的重组发生在8-bp核心序列（GCATACAT）中。" EBNA1 5487..7412 /codon_start=1 /product="Epstein-Barr nuclear antigen 1, also known as EBNA-1" /note="对于潜伏性病毒感染至关重要，并且对于含有oriP复制起点的载体的游离DNA扩增也是必不可少的（Okita等人，2013年）。" /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG AGGGAGAGGGAGGAGGAGGAGAGGGAG?GGG?GGAGGAGAGGGAGAGGGAGGAG?GGGG GG?GG?G?GGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAG?GG G?GG?GGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAG?GGAGGAG?GGG?GGAGAG GG?GG?GAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE GEEGQE" loxP complement(7580..7613) /bound_moiety="Cre recombinase" /note="Cre介导的重组发生在8-bp核心序列（GCATACAT）中。" oriP 7800..9589 /note="Epstein-Barr病毒oriP复制起点（Yates等人，2000年）" primer_bind complement(10130..10147) /note="L4440载体，正向引物" ori complement(10301..10889) /direction=LEFT /note="高拷贝数 ColE1/pMB1/pBR322/pUC 复制起点" primer_bind complement(10381..10400) /note="pBR322 复制起点，正向引物" AmpR complement(11060..11920) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="赋予对氨苄青霉素、卡那霉素和相关抗生素的抗性。" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 11683..11702 /note="氨苄青霉素抗性基因，反向引物" AmpR promoter complement(11921..12025) /gene="bla"
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