| Plasmid Name | pLKO.1-Puro | Alternative Names | DB00024;等效质粒Plasmid#8453;pLKO.1Puro |
|---|---|---|---|
| Promoter | U6 | Replicon | pUC |
| Copy Number | medium | Competent Cell | Stbl3 |
| Culture Temp | 37°C | Forward Primer | U6：ATGGACTATCATATGCTTACCGTA |
| Reverse Primer | Puro-R1：gagttcttgcagctcggtga | Storage Temp | 37°C |
1.For Research Use Only
All products available through the MiaoLing Plasmid Platform are intended for research use only. They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.
2.Plasmid Sequence and Experimental Performance
Plasmid products are provided and sold based on the corresponding reference/template plasmid. MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.
3.Sequence Verification of Certain Vector Products
For certain vector products, even the original manufacturer may not have fully verified the complete plasmid sequence. If the sequence of the key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.
4.After-Sales Period for Small-Pack Plasmid Products
The after-sales service period for small-pack plasmid products is three months from the date of receipt. Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.
5.Research-Related Risks and Limitation of Liability
Scientific research is inherently exploratory and involves a significant degree of uncertainty. To the maximum extent permitted by applicable law, MiaoLing shall not be liable for any additional economic losses, indirect losses, or other liabilities arising from the use of our products or from research outcomes.
6.Citation in Chinese Publications
For publications in Chinese, please cite the product as follows:
7.Product Name (Product Item Number) was provided by the MiaoLing Plasmid Platform.
Citation in English Publications
For publications in English, please cite the product as follows:
“Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”
PuroR 529..1128
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/note="赋予对普鲁霉素的抗性"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
3' LTR (Delta-U3) 1256..1489
/note="自失活型3'长末端重复序列（LTR）源自1型人类免疫缺陷病毒"
SV40 poly(A) signal 1561..1682
/note="SV40 polyA信号"
SV40 ori 1722..1857
/note="SV40复制起点"
T7 promoter complement(1878..1896)
/note="1型人类免疫缺陷病毒RNA聚合酶启动子"
f1 ori 2064..2519
/direction=RIGHT
/note="f1 噬菌体复制起点；箭头指示(+)链合成方向"
AmpR promoter 2545..2649
/gene="bla"
AmpR 2650..3510
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="赋予对氨苄青霉素、卡那霉素和相关抗生素的抗性。"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
pUC ori 3681..4269
/direction=RIGHT
/note="高拷贝数 ColE1/pMB1/pBR322/pUC 复制起点"
CAP binding site 4557..4578
/bound_moiety="E. coli catabolite activator protein"
/note="CAP结合激活了cAMP存在的条件下的转录。"
lac promoter 4593..4623
/note="大肠杆菌乳糖操纵子的启动子"
lac operator 4631..4647
/bound_moiety="lac repressor encoded by lacI"
/note="lac阻遏蛋白结合到lac操纵子上以抑制大肠杆菌中的转录。这种抑制可以通过添加乳糖或异丙基-β-D-硫代半乳糖苷（IPTG）来解除。"
T3 promoter 4692..4710
/note="T3 噬菌体 RNA 聚合酶的启动子"
RSV promoter 4738..4964
/note="禽类肉瘤病毒增强子/启动子"
5' LTR (truncated) 4965..5145
/note="截短型5'长末端重复序列（LTR），源自1型人类免疫缺陷病毒"
HIV-1 Psi 5192..5317
/note="packaging signal of human immunodeficiency virus
type 1"
RRE 5810..6043
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
U6 promoter 6570..6810
/note="RNA polymerase III promoter for human U6 snRNA"
cPPT/CTS 6881..6998
/note="1型人类免疫缺陷病毒的中心多嘌呤道和中心终止序列"
hPGK promoter join(7047..7050,1..507)
/note="人类磷酸甘油酸激酶1启动子" 