| Plasmid Name | 6-His-MBP-TEV-FnCpf1 | Alternative Names | 等效质粒Plasmid#90094 |
|---|---|---|---|
| Promoter | T7 | Replicon | pBR322 |
| Copy Number | middle | Competent Cell | Stbl3 |
| Culture Temp | 37°C | Forward Primer | T7： |
| Induction | IPTG induction | Storage Temp | 37°C |
1.For Research Use Only
All products available through the MiaoLing Plasmid Platform are intended for research use only. They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.
2.Plasmid Sequence and Experimental Performance
Plasmid products are provided and sold based on the corresponding reference/template plasmid. MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.
3.Sequence Verification of Certain Vector Products
For certain vector products, even the original manufacturer may not have fully verified the complete plasmid sequence. If the sequence of the key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.
4.After-Sales Period for Small-Pack Plasmid Products
The after-sales service period for small-pack plasmid products is three months from the date of receipt. Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.
5.Research-Related Risks and Limitation of Liability
Scientific research is inherently exploratory and involves a significant degree of uncertainty. To the maximum extent permitted by applicable law, MiaoLing shall not be liable for any additional economic losses, indirect losses, or other liabilities arising from the use of our products or from research outcomes.
6.Citation in Chinese Publications
For publications in Chinese, please cite the product as follows:
7.Product Name (Product Item Number) was provided by the MiaoLing Plasmid Platform.
Citation in English Publications
For publications in English, please cite the product as follows:
“Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”
ori 532..1120
/direction=RIGHT
/note="高拷贝数ColE1/pMB1/pBR322/pUC复制起点"
bom 1306..1445
/note="pBR322迁移区的基础"
rop complement(1547..1738)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
lacI complement(2547..3629)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/note="lac阻遏物与lac操作员结合，抑制大肠杆菌中的转录。这种抑制可以通过添加乳糖或异丙基β-D-硫代吡喃半乳糖苷（IPTG）来缓解。"
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
lacI promoter complement(3630..3707)
/gene="lacI"
T7 promoter 4016..4034
/note="噬菌体T7 RNA聚合酶启动子"
lac operator 4035..4059
/bound_moiety="lac repressor encoded by lacI"
/note="lac阻遏物与lac操作员结合，抑制大肠杆菌中的转录。这种抑制可以通过添加乳糖或异丙基β-D-硫代吡喃半乳糖苷（IPTG）来缓解。"
RBS 4074..4096
/note="噬菌体T7基因10的高效核糖体结合位点（Olins和Rangwala，1989）"
6xHis 4110..4127
/codon_start=1
/product="6xHis affinity tag"
/translation="HHHHHH"
MBP 4128..5243
thrombin site 5244..5261
/codon_start=1
/product="thrombin recognition and cleavage site"
Cleavage site after base 5255"
/translation="LVPRGS"
CDS 5268..5288
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
Cleavage site after base 5285"
/translation="ENLYFQG"
FnCpf1 (humanized) 5289..9188
nucleoplasmin NLS 9189..9236
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/translation="KRPAATKKAGQAKKKK"
3xHA 9243..9323
/codon_start=1
/product="three tandem HA epitope tags"
/translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
terminator 9516..9563
/note="噬菌体T7 RNA聚合酶的转录终止子"
f1 ori 9600..10055
/direction=RIGHT
/note="f1噬菌体复制起点；箭头表示（+）链合成的方向"
AmpR promoter 10081..10185
/gene="bla"
AmpR join(10186..10685,1..361)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="对氨苄青霉素、羧苄青霉素和相关抗生素产生耐药性"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW" 