| Plasmid Name | pBT268_(pCA-ATG-intron-tTA2-iiTRE-tdT3Mycii) | Alternative Names | 等效质粒Plasmid#36880 |
|---|---|---|---|
| Promoter | Tet | Replicon | pUC |
| Copy Number | high | Competent Cell | Stbl3 |
| Culture Temp | 30°C | Storage Temp | 30°C |
1.For Research Use Only
All products available through the MiaoLing Plasmid Platform are intended for research use only. They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.
2.Plasmid Sequence and Experimental Performance
Plasmid products are provided and sold based on the corresponding reference/template plasmid. MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.
3.Sequence Verification of Certain Vector Products
For certain vector products, even the original manufacturer may not have fully verified the complete plasmid sequence. If the sequence of the key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.
4.After-Sales Period for Small-Pack Plasmid Products
The after-sales service period for small-pack plasmid products is three months from the date of receipt. Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.
5.Research-Related Risks and Limitation of Liability
Scientific research is inherently exploratory and involves a significant degree of uncertainty. To the maximum extent permitted by applicable law, MiaoLing shall not be liable for any additional economic losses, indirect losses, or other liabilities arising from the use of our products or from research outcomes.
6.Citation in Chinese Publications
For publications in Chinese, please cite the product as follows:
7.Product Name (Product Item Number) was provided by the MiaoLing Plasmid Platform.
Citation in English Publications
For publications in English, please cite the product as follows:
“Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”
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f1 ori complement(6..461)
/direction=LEFT
/note="f1噬菌体复制起点；箭头表示（+）链合成的方向"
primer_bind 603..619
/note="常见的测序引物，多种相似变体之一"
T7 promoter 626..644
/note="噬菌体T7 RNA聚合酶启动子"
primer_bind 670..686
/note="常见的测序引物，多种相似变体之一"
CMV enhancer 791..1094
/note="人类巨细胞病毒即刻早期增强子"
chicken beta-actin promoter 1096..1373
intron 1374..2390
/note="鸡β肌动蛋白内含子与兔β珠蛋白嵌合体"
regulatory 2470..2479
/regulatory_class="other"
/note="脊椎动物强启动翻译的共识序列（Kozak，1987）"
loxP 2496..2529
/bound_moiety="Cre recombinase"
/note="Cre介导的重组发生在8-bp核心序列（GCATACAT）中。"
tTA-Advanced 2649..3392
/codon_start=1
/product="improved tetracycline-controlled transactivator"
/note="在Tet-Off（R）系统中，tTA-Advanced仅在没有四环素或强力霉素的情况下与Tet反应元件结合并刺激转录。"
/translation="SRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVK
NKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRP
TEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTD
SMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPAD
ALDDFDLDMLPADALDDFDLDMLPG"
SV40 poly(A) signal 3514..3635
/note="SV40聚腺苷酸化信号"
tetracycline response element 4159..4429
/bound_moiety="tetracycline repressor TetR"
/note="包含四环素算子tetO的七个拷贝"
minimal CMV promoter 4462..4500
/note="人巨细胞病毒（CMV）即刻早期启动子"
regulatory 4625..4634
/regulatory_class="other"
/note="脊椎动物强启动翻译的共识序列（Kozak，1987）"
CDS 4631..6058
/codon_start=1
/product="tandem dimeric (pseudo-monomeric) derivative of
DsRed (Shaner et al., 2004)"
/note="哺乳动物密码子优化"
/translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
RSEGRHHLFLYGMDELYK"
Myc 6068..6097
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/translation="EQKLISEEDL"
Myc 6104..6133
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/translation="EQKLISEEDL"
Myc 6140..6169
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/translation="EQKLISEEDL"
SV40 poly(A) signal 6307..6388
/note="SV40聚腺苷酸化信号"
T3 promoter complement(6955..6973)
/note="噬菌体T3 RNA聚合酶启动子"
primer_bind complement(6994..7010)
/note="常见的测序引物，多种相似变体之一"
lac operator 7018..7034
/bound_moiety="lac repressor encoded by lacI"
/note="lac阻遏物与lac操作员结合，抑制大肠杆菌中的转录。这种抑制可以通过添加乳糖或异丙基β-D-硫代吡喃半乳糖苷（IPTG）来缓解。"
lac promoter complement(7042..7072)
/note="大肠杆菌lac操纵子的启动子"
CAP binding site 7087..7108
/bound_moiety="E. coli catabolite activator protein"
/note="CAP结合在cAMP存在下激活转录。"
ori complement(7396..7984)
/direction=LEFT
/note="高拷贝数ColE1/pMB1/pBR322/pUC复制起点"
AmpR complement(8155..9015)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="对氨苄青霉素、羧苄青霉素和相关抗生素产生耐药性"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
AmpR promoter complement(9016..9120)
/gene="bla" 