| Plasmid Name | pEYFP-C1 | Promoter | CMV |
|---|---|---|---|
| Replicon | pUC | Copy Number | high |
| Competent Cell | DH5a | Culture Temp | 37°C |
| Forward Primer | CMV-F： | Storage Temp | 37°C |
1.For Research Use Only
All products available through the MiaoLing Plasmid Platform are intended for research use only. They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.
2.Plasmid Sequence and Experimental Performance
Plasmid products are provided and sold based on the corresponding reference/template plasmid. MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.
3.Sequence Verification of Certain Vector Products
For certain vector products, even the original manufacturer may not have fully verified the complete plasmid sequence. If the sequence of the key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.
4.After-Sales Period for Small-Pack Plasmid Products
The after-sales service period for small-pack plasmid products is three months from the date of receipt. Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.
5.Research-Related Risks and Limitation of Liability
Scientific research is inherently exploratory and involves a significant degree of uncertainty. To the maximum extent permitted by applicable law, MiaoLing shall not be liable for any additional economic losses, indirect losses, or other liabilities arising from the use of our products or from research outcomes.
6.Citation in Chinese Publications
For publications in Chinese, please cite the product as follows:
7.Product Name (Product Item Number) was provided by the MiaoLing Plasmid Platform.
Citation in English Publications
For publications in English, please cite the product as follows:
“Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”
CMV enhancer 61..364
/note="人类巨细胞病毒即刻早期增强子"
CMV promoter 365..568
/note="人巨细胞病毒（CMV）即刻早期启动子"
primer_bind 600..639
regulatory 607..616
/regulatory_class="other"
/note="脊椎动物强启动翻译的共识序列（Kozak，1987）"
EYFP 613..1329
/codon_start=1
/product="enhanced YFP"
/note="哺乳动物密码子优化"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(1301..1348)
MCS 1330..1395
/note="多克隆位点"
SV40 poly(A) signal 1519..1640
/note="SV40聚腺苷酸化信号"
f1 ori complement(1647..2102)
/direction=LEFT
/note="f1噬菌体复制起点；箭头表示（+）链合成的方向"
AmpR promoter 2129..2233
/gene="bla"
SV40 promoter 2235..2592
/note="SV40增强子和早期启动子"
SV40 ori 2443..2578
/note="SV40复制起点"
NeoR/KanR 2627..3421
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="赋予对新霉素、卡那霉素和G418（遗传霉素（R））的抗性"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
HSV TK poly(A) signal 3653..3700
/note="单纯疱疹病毒胸苷激酶多聚腺苷酸化信号（Cole和Stacy，1985）"
ori 4029..4617
/direction=RIGHT
/note="高拷贝数ColE1/pMB1/pBR322/pUC复制起点" 