产品声明
For Research Use Only research use only . They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.Plasmid Sequence and Experimental Performance reference/template plasmid . MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.Sequence Verification of Certain Vector Products key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.After-Sales Period for Small-Pack Plasmid Products three months from the date of receipt . Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.Research-Related Risks and Limitation of Liability Citation in Chinese Publications 产品名称（产品货号）由淼灵质粒平台提供。 Citation in English Publications “Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”
质粒序列
一键复制序列（有部分质粒是非全长序列）
GTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATACGCGTTGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCTGGCTAGCGTTTAAACTTAAGCTTGGTACCGAGCTCGGATCCACTAGTCCAGTGTGGTGGAATTCGCCACCATGCGGGCGCAGGGCTCTGGCCTGTGCGCCTTGGTGCTGCTGGCCTGGCTGGCGGGCACCGTGTACCCCTGGGCACTTGCCAGCTGGGACCCGGGTCTGGCACCCATGACCCCCCGGAACAGCAGCGGGGTCCCGGGGGCCAGCTTGCCGTGGCTGTCCGTGCGGGTCCCCTTCCTCCTCACGCTGTGGACACTCGTGGCCCTGTCAGCCCTCCTGGGCTTCCACCTGTCCCACAAGGTCACCAGCGTCGTCCCTGAGAGCGCACTGCTCATCGTGCTGGGCCTGGTGCTGGGCGGCATCGTCTGGGCGGCCGACCACATCGCCTCCTTCACCCTCACGCCCACCCTCTTCTTCTTCTACCTGCTGCCCCCCATCGTGCTGGACGCCGGCTACTTCATGCCCAACCGGCTCTTCTTTGGCAACCTGGGCACCATCCTCCTCTATGCCGTCATTGGCACCGTGTGGAACGCGGCCGCCACCGGCCTGTCACTCTACGGCGTCTTCCTCAGTGGCCTGATGGGGGACCTGGACATCGGGCTGCTGGACTTCCTGCTGTTCGGCAGCCTGATCGCCGCCGTGGACCCGGTGGCCGTCCTGGCCGTGTTCGAGGAGGTGCACGTCAACGAGGTGCTCTTCATCATCGTCTTCGGGGAGTCGCTGCTCAACGACGCCGTCACGGTGGTCCTATACAACGTGTTTGAATCTTTCGTGACGCTGGGGGGTGACAAGGTGACCGGCGTGGACTGCGTGAAGGGATTAGTGTCCTTCTTCGTGGTGAGCCTGGGGGGCACGCTGGTGGGGGTCGTCTTCGCCTTCCTGCTCTCGCTGGTAACGCGCTTCACCAAGCACGTGCGCACCATCGAGCCCGGCTTCGTGTTCGTGCTGTCCTACCTGTCCTACCTCACGTCCGAGATGCTGTCGCTGTCGGCCATCCTGGCCATCACCTTCTGCGGCATCTGCTGCCAGAAGTACGTGAAGGCCAACATCTCGGAGCAGTCCGCCACCACCGTGCGCTACACCATGAAGATGCTGGCCAGCGGCGCCGAGACCATCATCTTCATGTTCCTGGGCATCTCGGCCGTGGACCCGCTCATCTGGAAGTGGAACACAGCCTTTGTGCTCCTGACGCTGGTCTTTATCTCCGTGTACCGGGTCATCGGTGTGGTGCTGCAGACCTGGATCCTGAACCGGTACCGGATGGTGCAGCTGGAGATCATAGACCAGGTGGTCATGTCCTACGGCGGCCTCCGCGGGGCGGTGGCCTTCGCCCTGGTGGTCCTTCTGGACGAGAACAAGGTCAAGGAGAAGAACCTGTTCGTCAGCACCACCCTCATCGTCATCTTCTTCACCGTCATCGTCCAGGGCCTGACCATCAAGCCTCTGGTGCAGTGGCTGAAGGTGAAGAGGAGTGAACAGCGAGAGCCCAAGCTCAATGAGAAGCTGCATGGCCGGGCTTTCGACCACATCCTCTCGGCCATCGAGGACATTTCAGGGCAAATTGGACACAACTATCTCAGAGACAAGTGGTCCAATTTTGATCGGAAATTCCTCAGCAAAATCCTTATGAGAAGGTCGGCTCAAAAGTCACGAGATCGGATTCTGAATGTTTTCCACGAGCTCAACTTGAAGGACGCCATCAGCTACGTGGCCGAGGGAGAGCGCCGAGGCTCCCTGGCCTTCATCCGCTCCCCCAGCACCGACAACATGGTCAACGTGGACTTCAGCACCCCCAGACCCTCGACCGTTGAGGCCTCCATCTCCTACCTCCTGAGGGAGAATGTCAGCGCCGTGCGGCTGGACATGCAGTCGCTGGAGCAGAGGCGGCAGAGCATCCGTGACACGGAGGACATGGTCACGCACCACACGCTGCAGCAGTACCTGTACAAGCCCCGGCAGGAGTACAAACACCTCTACAGCCGACACGAGTTGACGGCAGACGAACACGAGAAGCAGGACAAGGAGGTCTTCCACAGGACCATGCGCAAACGCCTGGAGTCCTTCAAGTCGGCCAAGCTGGGGCTCCACCCGCACAAGAAGGGGGCGAAGCTGTACAAGAGGGAGCGCGCCCAGAAGCGGAGAAACAGCAGCATCCCCAACGGGAAACTGCCGATGGGAAGCTCCCTGCAGGATTTCACCATTAAGGAGAGAGATTTGGAACTTTTGGACCCCGAGGAAGCCGCCAGCTATGATGCCGAGGACATGAGCGGGGGGATCGAGTTCCTGGCAAACATCACACAGGACACAGCGACAGACTCCCCCTCCGGAATTGACAACCCCGTGTTTTCCCCCGACGAGGACCCCAGCATCCTCAGCCGGGCACCGCCCTGGCTGTCTCCCGGGGAGGCAGTGGTGCCCTCCCAGAGGGCCCGAGTGCAGATCCCCCACTCTCCCGGCAACTTCCACCGCCTGGCGCCCTTCCGCCTCAGCTGCAAGTCCGTGGACTCCTTCGTGCTGGCCGATGGCTCCGAGGAGCAGCCCCGCGCCGCCCCGGCCGAGTCCACACACATGCTCGAGGACTACAAAGACCATGACGGTGATTATAAAGATCATGACATCGACTACAAGGATGACGATGACAAGTAGTGAGGGCCCGATATCTCGACAATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTGCTGTCTCTTTATGAGGAGTTGTGGCCCGTTGTCAGGCAACGTGGCGTGGTGTGCACTGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCATTGCCACCACCTGTCAGCTCCTTTCCGGGACTTTCGCTTTCCCCCTCCCTATTGCCACGGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGTGTTGTCGGGGAAGCTGACGTCCTTTCCATGGCTGCTCGCCTGTGTTGCCACCTGGATTCTGCGCGGGACGTCCTTCTGCTACGTCCCTTCGGCCCTCAATCCAGCGGACCTTCCTTCCCGCGGCCTGCTGCCGGCTCTGCGGCCTCTTCCGCGTCTTCGCCTTCGCCCTCAGACGAGTCGGATCTCCCTTTGGGCCGCCTCCCCGCCTGGAAACGGGGGAGGCTAACTGAAACACGGAAGGAGACAATACCGGAAGGAACCCGCGCTATGACGGCAATAAAAAGACAGAATAAAACGCACGGGTGTTGGGTCGTTTGTTCATAAACGCGGGGTTCGGTCCCAGGGCTGGCACTCTGTCGATACCCCACCGAGACCCCATTGGGGCCAATACGCCCGCGTTTCTTCCTTTTCCCCACCCCACCCCCCAAGTTCGGGTGAAGGCCCAGGGCTCGCAGCCAACGTCGGGGCGGCAGGCCCTGCCATAGCAGATCTGCGCAGCTGGGGCTCTAGGGGGTATCCCCACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGCATCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGGGGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTAATTCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTCCCGGGAGCTTGTATATCCATTTTCGGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGCGAAATGACCGACCAAGCGACGCCCAACCTGCCATCACGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGTTTTCCGGGACGCCGGCTGGATGATCCTCCAGCGCGGGGATCTCATGCTGGAGTTCTTCGCCCACCCCAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGTATACCGTCGACCTCTAGCTAGAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCGACGGATCGGGAGATCTCCCGATCCCCTATGGTCGACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCGCGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTGCTTAGG
质粒元件
CMV enhancer 50..429
/note="人巨细胞病毒即刻早期增强子"
CMV promoter 430..633
/note="人巨细胞病毒（CMV）即刻早期启动子"
primer_bind 694..729
SLC9A3-p 706..3213
/codon_start=1
/translation="MRAQGSGLCALVLLAWLAGTVYPWALASWDPGLAPMTPRNSSGVP
GASLPWLSVRVPFLLTLWTLVALSALLGFHLSHKVTSVVPESALLIVLGLVLGGIVWAA
DHIASFTLTPTLFFFYLLPPIVLDAGYFMPNRLFFGNLGTILLYAVIGTVWNAAATGLS
LYGVFLSGLMGDLDIGLLDFLLFGSLIAAVDPVAVLAVFEEVHVNEVLFIIVFGESLLN
DAVTVVLYNVFESFVTLGGDKVTGVDCVKGLVSFFVVSLGGTLVGVVFAFLLSLVTRFT
KHVRTIEPGFVFVLSYLSYLTSEMLSLSAILAITFCGICCQKYVKANISEQSATTVRYT
MKMLASGAETIIFMFLGISAVDPLIWKWNTAFVLLTLVFISVYRVIGVVLQTWILNRYR
MVQLEIIDQVVMSYGGLRGAVAFALVVLLDENKVKEKNLFVSTTLIVIFFTVIVQGLTI
KPLVQWLKVKRSEQREPKLNEKLHGRAFDHILSAIEDISGQIGHNYLRDKWSNFDRKFL
SKILMRRSAQKSRDRILNVFHELNLKDAISYVAEGERRGSLAFIRSPSTDNMVNVDFST
PRPSTVEASISYLLRENVSAVRLDMQSLEQRRQSIRDTEDMVTHHTLQQYLYKPRQEYK
HLYSRHELTADEHEKQDKEVFHRTMRKRLESFKSAKLGLHPHKKGAKLYKRERAQKRRN
SSIPNGKLPMGSSLQDFTIKERDLELLDPEEAASYDAEDMSGGIEFLANITQDTATDSP
SGIDNPVFSPDEDPSILSRAPPWLSPGEAVVPSQRARVQIPHSPGNFHRLAPFRLSCKS
VDSFVLADGSEEQPRAAPAESTHM"
primer_bind complement(3189..3219)
CDS 3220..3285
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
Cleavage site after base 3285"
/translation="DYKDHDGDYKDHDIDYKDDDDK"
WPRE 3309..3897
/note="土拨鼠肝炎病毒转录后调控元件"
Factor Xa site complement(3780..3791)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
Cleavage site after base 3779"
/translation="IEGR"
HSV TK poly(A) signal 3963..4011
/note="单纯疱疹病毒胸苷激酶多聚腺苷酸化信号（Cole和Stacy，1985）"
f1 ori 4213..4641
/direction=RIGHT
/note="f1噬菌体复制起点；箭头表示（+）链合成的方向"
SV40 promoter 4655..4984
/note="SV40增强子和早期启动子"
SV40 ori 4835..4970
/note="SV40复制起点"
NeoR/KanR 5051..5845
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="赋予对新霉素、卡那霉素和G418（遗传霉素（R））的抗性"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
SV40 poly(A) signal 6021..6142
/note="SV40聚腺苷酸化信号"
primer_bind complement(6191..6207)
/note="常见的测序引物，多种相似变体之一"
lac operator 6215..6231
/bound_moiety="lac repressor encoded by lacI"
/note="lac阻遏物与lac操作员结合，抑制大肠杆菌中的转录。这种抑制可以通过添加乳糖或异丙基β-D-硫代吡喃半乳糖苷（IPTG）来缓解。"
lac promoter complement(6239..6269)
/note="大肠杆菌lac操纵子的启动子"
CAP binding site 6284..6305
/bound_moiety="E. coli catabolite activator protein"
/note="CAP结合在cAMP存在下激活转录。"
ori complement(6593..7181)
/direction=LEFT
/note="高拷贝数ColE1/pMB1/pBR322/pUC复制起点"
AmpR complement(7352..8212)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="对氨苄青霉素、羧苄青霉素和相关抗生素产生耐药性"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
AmpR promoter complement(8213..8317)
/gene="bla"
