产品声明
For Research Use Only research use only . They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.Plasmid Sequence and Experimental Performance reference/template plasmid . MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.Sequence Verification of Certain Vector Products key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.After-Sales Period for Small-Pack Plasmid Products three months from the date of receipt . Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.Research-Related Risks and Limitation of Liability Citation in Chinese Publications 产品名称（产品货号）由淼灵质粒平台提供。 Citation in English Publications “Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”
质粒序列
一键复制序列（有部分质粒是非全长序列）
GCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTTCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAGCGCGCGTAATACGACTCACTATAGGGCGAATTGGGTACAAGCTTGGATTTATTTTGTTATGTTATATGTATTATATGTCAGACATAAAGAAAAGGAACACATCAAATGTGATAACAAAGACTAAACAAGTAATTTTATTACACCAAAACGACAAAACAGTAGGCAGAACAAACAACGCATAGCCAAACATTGACGAATTGGATACCCTGCCGATTGTCAGACACTTTTGTTGATCAGTTTCTTGCGAATGGTCTCGTCCAGCGGTGGAATCGCCTCGCGGGGAATCAGAAAAGTGGACAGATTGAACAGATCCAGAAACACCTTGTACCGATCACTGAAACCAAAAAAAAACAAAGGGAGAACAGTTTGAGTTCATTGATCCCCGATATAATCACATCTGCGATGATCACCTGAGAGTGGAGCGCAGATATTGATATCCAGACGAGCCACCAGTGCCCAACTGTTGGGATCCAATCATGCGTTGCACCATGATCACGTGATTGTCTGCGGCGGGAATAGAAAGTATTTGGTTAGGAAAACCAGTCTTAAACATAAGATATATTTATAAAAGAGTATCAAAGAATGCAATACTTACATCTCCACTTGGTTATTAACGAGTCGATGTCCATGAGCAGGGTGAGCAACTGGTGTGGTTGGCTGAACCTGGGTTCATCCCTATAGAAGGTGATCATGATGGCTCCCTGAAGGGCACGATGGCTAAACCGGCGATCCCCACGACGCACCAGTGCATCGTGCACTGCCGGATCAAAGATGGAGCGATACACCTCGCGTCGCTTCTCAATGTCCATGAGGCGGTAGTTTTTCGCCTTCTCCACGGGCTCCTCCATGGCGCTCTGTACCTGCGCCTCCAGGAATCGATCGACGCTCTCCTGAAACTTGGCCCAGAAGTTGAAGCCACTCTCCTCCAGTCCGGGCGTCCTCTCCAGCCATCGCTGCACTAGCTCCAGTAGCGAGGGATCTTTCTCCGAGTTGCGAATCGAGTTCCGCGCCTCCTCGTCGCTAAAGACATCCGAGTACTTCTGGTTGTATCTCACCCGCTGCTCTGTCAGAACTCCCAGCTTGTTCTCGATCAAACGGAACTGCAGCGACTGAAAACCAGATGCGGGTGCCAGGTACTTGCGGAAGTCCATGAAGTCTAGCGGGGTCATGGTCTCCAGAATGGGCACTTGGTCCACCAGGAGCTGTACAAAGGAAGTTATAAACGGATTTTGGTAAGAGATTCAGAAAGCACTCACTTTTAGAATCAGAACCACTCGGTTCAGTCGCTTGACAATCTCCAGCGTCTTGGTTTCATCGATGACCTCTGCATCCAACATGTCTCGTATGGAGTCGAACTCAAAGATGATCTGCTTGAACCAAAGCTCGTAGGCTGTGGCGAAGGTACTTAAATGCCATTGAGTGTTGTCATCAAAGTTGTAAACCTACTCACCCTGGTGCGTGATGATGAACAGATGCTCATCGTGCACGGGTCGCTTGTCCTCCTCGGACAGCATACACTGGGCATCCAGCAGTTTGTCCAGCATCAGATACTCTCCATAGATTTTGCCCACTTCCGTGGTTAATGGCACCGCCGAATCATCGTGATCGTTTCTGTATGGGTTTGAATTGAATCGCAGAACTGAAGATCGATTGGCATTCCTGGACAGCACGTGCTGGTGCTCACCCGTTTCCTGCATAGGGACAGCTCATGGTGCACAGCTCAGATCAGATCGTGACTCCTCGAGCGGCGGATGCTGGCGAACTGATCTCCGCCAGCGGACCGGAGATGAGACCCCAGCGAACCGATAACAGAGCGAGAGAGCTCCAGTTCCGACTGATTGCACAGTCGGTGATCTGGGCGATGGGCACTGCCAGATAGGCTGGGAATTATCAATCACTTGAGGTGAAAGTGCGGCGCACACAAATCCAAGCTTGATATCATCGATCTCGAGGCTGCATCCAACGCGTTGGGAGCTCTCCGGATCAATTCGGCTTCAGGTACCGTCGACGATGTAGGTCACGGTCTCGAAGCCGCGGTGCGGGTGCCAGGGCGTGCCCTTGGGCTCCCCGGGCGCGTACTCCACCTCACCCATCTGGTCCATCATGATGAACGGGTCGAGGTGGCGGTAGTTGATCCCGGCGAACGCGCGGCGCACCGGGAAGCCCTCGCCCTCGAAACCGCTGGGCGCGGTGGTCACGGTGAGCACGGGACGTGCGACGGCGTCGGCTGGTGCGGATACGCGGGGCAGCGTCAGCGGGTTCTCGACGGTCACGGCGGGCATGTCGACAAGCCGAATTGATCCACTAGAAGGCCTAATTCGGTACACTAGTTGGCCACGTAATAAGTGTGCGTTGAATTTATTCGCAAAAACATTGCATATTTTCGGCAAAGTAAAATTTTGTTGCATACCTTATCAAAAAATAAGTGCTGCATACTTTTTAGAGAAACCAAATAATTTTTTATTGCATACCCGTTTTTAATAAAATACATTGCATACCCTCTTTTAATAAAAAATATTGCATACTTTGACGAAACAAATTTTCGTTGCATACCCAATAAAAGATTATTATATTGCATACCCGTTTTTAATAAAATACATTGCATACCCTCTTTTAATAAAGAATATTGCATACGTTGACGAAACAAATTTTCGTTGCATACCCAATAAAAGATTATTATATTGCATACCTTTTCTTGCCATACCATTTAGCCGATCAATTGAGATCTGAATTCTTTTTTGCTCACCTGTGATTGCTCCTACTCAAATACAAAAACATCAAATTTTCTGTCAATAAAGCATATTTATTTATATTTATTTTACAGGAAAGAATTCCTTTTAAAGTGTATTTTAACCTATAATGAAAAACGATTAAAAAAAATACATAAAATAATTCGAAAATTTTTGAATAGCCCAGGTTGATAAAAATTCATTTCATACGTTTTATAACTTATGCCCCTAAGTATTTTTTGACCATAGTGTTTCAATTCTACATTAATTTTACAGAGTAGAATGAAACGCCACCTACTCAGCCAAGAGGCGAAAAGGTTAGCTCGCCAAGCAGAGAGGGCGCCAGTGCTCACTACTTTTTATAATTCTCAACTTCTTTTTCCAGACTCAGTTCGTATATATAGACCTATTTTCAATTTAACGTCGGGGCTTTGAGTGTGTGTAGACATCAAGCATCGGTGGTTCAGTGGTAGAATGCTCGCCTGCCACGCGGGCGGCCCGGGTTCGATTCCCGGCCGATGCAGGGTCTTCGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGCTAACAAAGCACCAGTGGTCTAGTGGTAGAATAGTACCCTGCCACGGTACAGACCCGGGTTCGATTCCCGGCTGGTGCAGAAGACCTGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTGCCTACCTGGAGCCTGAGAGTTGTTCAATAAAATAAAAATGTTTCGTTTTTTTGCTTTCGCCAGTATTTATTATTTTTCATCAATATGTATTCAATTTGGTATGTATTTAGTAATTGTAATATATAGACAATGGTTTTCCGTTGACGTACATACATCTGACGTGTGTTTATTTAGACATAATAGTTATGTTTTCACATCTTTTTAATGTTCGCTTAATGCGTATGCATTCTAGACAATTGTGCTCGGCAACAGTATATTTGTGGTGTGCCAACCAACAACCTGCAGGAGCTCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTT
质粒元件
ori complement(196..784)
/direction=LEFT
/note="高拷贝数ColE1/pMB1/pBR322/pUC复制起点"
AmpR complement(955..1815)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="对氨苄青霉素、羧苄青霉素和相关抗生素产生耐药性"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
AmpR promoter complement(1816..1920)
/gene="bla"
f1 ori 1947..2403
/direction=RIGHT
/note="f1噬菌体复制起点；箭头表示（+）链合成的方向"
primer_bind 2544..2560
/note="常见的测序引物，多种相似变体之一"
T7 promoter 2570..2588
/note="噬菌体T7 RNA聚合酶启动子"
vermilion complement(join(2780..2897,2974..3067,3159..3765,3820..3953,
4014..4174,4248..4273))
/codon_start=1
/product="tryptophan oxygenase"
/note="果蝇棕眼色素合成所需的选择性标记"
/translation="MSCPYAGNGNDHDDSAVPLTTEVGKIYGEYLMLDKLLDAQCMLSE
EDKRPVHDEHLFIITHQAYELWFKQIIFEFDSIRDMLDAEVIDETKTLEIVKRLNRVVL
ILKLLVDQVPILETMTPLDFMDFRKYLAPASGFQSLQFRLIENKLGVLTEQRVRYNQKY
SDVFSDEEARNSIRNSEKDPSLLELVQRWLERTPGLEESGFNFWAKFQESVDRFLEAQV
QSAMEEPVEKAKNYRLMDIEKRREVYRSIFDPAVHDALVRRGDRRFSHRALQGAIMITF
YRDEPRFSQPHQLLTLLMDIDSLITKWRYNHVIMVQRMIGSQQLGTGGSSGYQYLRSTL
SDRYKVFLDLFNLSTFLIPREAIPPLDETIRKKLINKSV"
attB 4588..4657
/bound_moiety="phage phi-C31 integrase"
/note="phi-C31整合酶的attB位点（Groth等人，2000）"
gypsy insulator 4899..5284
/bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
/note="果蝇染色质绝缘体"
dU6-3 promoter 5298..5718
/note="果蝇U6-3 snRNA的RNA聚合酶III启动子（Port等人，2014）"
misc_RNA 5824..5899
/note="化脓性链球菌CRISPR/Cas9系统的RNA支架"
misc_RNA 5986..6061
/note="化脓性链球菌CRISPR/Cas9系统的RNA支架"
T3 promoter complement(6372..6390)
/note="噬菌体T3 RNA聚合酶启动子"
primer_bind complement(6411..6427)
/note="常见的测序引物，多种相似变体之一"
lac operator 6435..6451
/bound_moiety="lac repressor encoded by lacI"
/note="lac阻遏物与lac操作员结合，抑制大肠杆菌中的转录。这种抑制可以通过添加乳糖或异丙基β-D-硫代吡喃半乳糖苷（IPTG）来缓解。"
lac promoter complement(6459..6489)
/note="大肠杆菌lac操纵子的启动子"
CAP binding site 6504..6525
/bound_moiety="E. coli catabolite activator protein"
/note="CAP结合在cAMP存在下激活转录。"
