For Research Use Only All products available through the MiaoLing Plasmid Platform are intended for research use only. They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.
Plasmid Sequence and Experimental Performance Plasmid products are provided and sold based on the corresponding reference/template plasmid. MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.
Sequence Verification of Certain Vector Products For certain vector products, even the original manufacturer may not have fully verified the complete plasmid sequence. If the sequence of the key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.
After-Sales Period for Small-Pack Plasmid Products The after-sales service period for small-pack plasmid products is three months from the date of receipt. Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.
Research-Related Risks and Limitation of Liability Scientific research is inherently exploratory and involves a significant degree of uncertainty. To the maximum extent permitted by applicable law, MiaoLing shall not be liable for any additional economic losses, indirect losses, or other liabilities arising from the use of our products or from research outcomes.
Citation in Chinese Publications For publications in Chinese, please cite the product as follows: 产品名称（产品货号）由淼灵质粒平台提供。
Citation in English Publications For publications in English, please cite the product as follows: “Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”
EF-1-alpha core promoter 31..242
/note="人延长因子EF-1α的核心启动子"
5' LTR (truncated) 255..523
/note="人T细胞白血病病毒1型截短5'长末端重复序列"
sig_peptide 573..632
/product="human interleukin-2 signal sequence"
Cleavage site after base 632"
CDS 654..1349
/codon_start=1
/product="CH2 and CH3 domains of the mouse IgG2a heavy
chain plus the hinge region"
/translation="PRGPTIKPCPPCKCPAPNLLGGPSVFIFPPKIKDVLMISLSPIVT
CVVVDVSEDDPDVQISWFVNNVEVHTAQTQTHREDYNSTLRVVSALPIQHQDWMSGKEF
KCKVNNKDLPAPIERTISKPKGSVRAPQVYVLPPPEEEMTKKQVTLTCMVTDFMPEDIY
VEWTNNGKTELNYKNTEPVLDSDGSYFMYSKLRVEKKNWVERNSYSCSVVHEGLHNHHT
TKSFSRTPGK"
SV40 poly(A) signal 1377..1498
/note="SV40聚腺苷酸化信号"
polyA_site 1602..1996
/note="人β-珠蛋白多聚腺苷酸化信号"
BleoR complement(2010..2384)
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/note="对博莱霉素、静脉霉素和Zeocin（TM）具有抗性"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
CMV enhancer 2977..3280
/note="人类巨细胞病毒即刻早期增强子"
ori complement(3360..3948)
/direction=LEFT
/note="高拷贝数ColE1/pMB1/pBR322/pUC复制起点"
polyA_signal 4056..4104
/note="合成聚腺苷酸化信号"
pause site 4115..4206
/note="人α2珠蛋白基因的RNA聚合酶II转录暂停信号"
