产品声明
For Research Use Only research use only . They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.Plasmid Sequence and Experimental Performance reference/template plasmid . MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.Sequence Verification of Certain Vector Products key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.After-Sales Period for Small-Pack Plasmid Products three months from the date of receipt . Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.Research-Related Risks and Limitation of Liability Citation in Chinese Publications 产品名称（产品货号）由淼灵质粒平台提供。 Citation in English Publications “Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”
质粒序列
一键复制序列（有部分质粒是非全长序列）
GGCAGAGGAGTAGGTAGAGCAACGAATCCTACTATTTATCCAAATTAGTCTAGGAACTCTTTTTCTAGATTTTTTAGATTTGAGGGCAAGCGCTGTTAACGACTCAGAAATGTAAGCACTACGGAGTAGAACGAGAAATCCGCCATAGGTGGAAATCCTAGCAAAATCTTGCTTACCCTAGCTAGCCTCAGGTAAGCTAGCCTTAGCCTGTCAAATTTTTTTCAAAATTTGGTAAGTTTCTACTAGCAAAGCAAACACGGTTCAACAAACCGAAAACTCCACTCATTATACGTGGAAACCGAAACAAAAAAACAAAAACCAAAATACTCGCCAATGAGAAAGTTGCTGCGTTTCTACTTTCGAGGAAGAGGAACTGAGAGGATTGACTACGAAAGGGGCAAAAACGAGTCGTATTCTCCCATTATTGTCTGCTACCACGCGGTCTAGTAGAATAAGCAACCAGTCAACGCTAAGACAGGTAATCAAAATACCAGTCTGCTGGCTACGGGCTAGTTTTTACCTCTTTTAGAACCCACTGTAAAAGTCCGTTGTAAAGCCCGTTCTCACTGTTGGCGTTTTTTTTTTTTTGGTTTAGTTTCTTATTTTTCATTTTTTTCTTTCATGACCAAAAACAAACAAATCTCGCGATTTGTACTGCGGCCACTGGGGCGTGGCCAAAAAAATGACAAATTTAGAAACCTTAGTTTCTGATTTTTCCTGTTATGAGGAGATATGATAAAAAATATTACTGCTTTATTGTTTTTTTTTTATCTACTGAAATAGAGAAACTTACCCAAGGAGGAGGCAAAAAAAAGAGTATATATACAGCAGCTACCATTCAGATTTTAATATATTCTTTTCTCTTCTTCTACACTATTATTATAATAATTTTACTATATTCATTTTTAGCTTAAAACCTCATAGAATATTATTCTTCAGTCACTCGCTTAAATACTTATCAAAAATGTCTAAGTTGGAAAAATTCACCAACTGTTACTCTTTGTCTAAGACTTTGAGATTCAAGGCCATCCCAGTTGGTAAGACCCAAGAAAACATCGACAACAAGAGACTATTAGTTGAAGATGAAAAGAGAGCTGAAGACTACAAGGGTGTCAAGAAATTGTTGGACAGATACTACTTGTCTTTTATCAACGACGTTTTGCATTCCATCAAGCTAAAGAACTTGAATAACTACATCTCTTTGTTCAGAAAGAAGACTAGAACTGAAAAGGAAAATAAGGAATTGGAAAACTTGGAAATCAACTTGAGAAAGGAAATTGCTAAGGCTTTCAAGGGTAATGAAGGTTACAAGTCTTTATTCAAGAAAGACATCATTGAAACCATTTTGCCAGAATTTTTGGATGATAAGGATGAAATTGCTTTGGTTAACTCTTTCAACGGTTTCACCACTGCTTTCACTGGTTTCTTCGACAACAGAGAAAACATGTTCTCCGAGGAAGCTAAATCCACTTCTATTGCTTTCAGATGTATCAACGAAAACTTGACCCGTTACATCTCTAACATGGACATTTTTGAAAAGGTCGACGCCATCTTTGACAAGCACGAAGTCCAAGAAATCAAGGAAAAGATCTTAAACTCCGACTACGATGTCGAAGATTTCTTCGAAGGTGAATTCTTCAACTTTGTTTTAACCCAAGAAGGTATCGATGTCTACAACGCCATTATCGGTGGTTTTGTCACTGAATCTGGTGAAAAGATCAAGGGTTTGAACGAATACATTAACTTGTACAACCAAAAGACCAAACAAAAATTGCCAAAGTTCAAGCCATTGTACAAGCAAGTTTTGTCTGACAGAGAATCTTTGTCTTTTTACGGTGAAGGGTACACCTCTGACGAAGAAGTCTTGGAAGTCTTCAGAAACACTTTGAACAAGAACTCTGAAATCTTCTCCTCCATCAAGAAGTTAGAAAAGTTGTTCAAGAACTTCGATGAATACTCTTCTGCTGGTATCTTCGTTAAGAACGGTCCAGCCATCTCTACCATTTCTAAGGATATCTTTGGTGAATGGAACGTCATTAGAGACAAATGGAACGCTGAATACGATGACATCCATTTGAAGAAAAAGGCTGTTGTCACCGAAAAGTACGAAGACGACAGAAGAAAATCCTTCAAGAAGATCGGTTCCTTCTCCTTGGAACAATTACAAGAATACGCCGATGCCGATTTGTCCGTTGTCGAAAAATTGAAGGAAATTATTATTCAAAAGGTTGATGAAATTTACAAAGTTTACGGTTCCTCTGAAAAGTTATTCGATGCTGATTTCGTCTTGGAAAAGTCTTTGAAGAAGAACGACGCTGTTGTCGCTATCATGAAGGACTTGTTGGACTCTGTCAAATCTTTCGAAAACTATATCAAGGCCTTCTTCGGTGAAGGTAAGGAAACTAACAGAGATGAATCCTTCTACGGTGACTTTGTCTTGGCTTACGATATTTTGTTGAAGGTTGACCACATCTACGATGCCATCAGAAACTACGTTACTCAAAAGCCATACTCTAAGGACAAATTCAAGTTGTACTTCCAAAACCCACAATTCATGGGTGGTTGGGATAAGGACAAGGAAACTGACTACAGAGCTACCATTTTGAGATACGGTTCCAAGTACTACTTGGCCATCATGGACAAGAAGTACGCCAAGTGTTTGCAAAAGATTGACAAGGACGATGTCAACGGTAACTACGAAAAGATTAACTACAAGTTGTTGCCAGGTCCAAACAAGATGTTGCCAAAGGTTTTCTTCTCCAAAAAGTGGATGGCTTACTACAACCCATCTGAAGACATCCAAAAGATCTACAAGAACGGTACTTTCAAAAAGGGTGACATGTTCAACTTAAACGACTGTCACAAGTTGATCGACTTCTTCAAGGACTCCATCTCTAGATACCCGAAATGGTCCAACGCTTACGATTTCAACTTCTCTGAAACTGAAAAATACAAGGATATTGCTGGTTTCTACCGTGAAGTCGAGGAACAAGGTTATAAGGTTTCTTTCGAATCCGCTTCTAAGAAAGAAGTTGACAAATTAGTCGAAGAAGGTAAGTTGTACATGTTCCAAATCTACAACAAAGATTTCTCCGACAAGTCTCACGGTACTCCAAACTTGCACACCATGTACTTCAAGTTGCTATTCGATGAAAACAACCACGGTCAAATCAGATTGTCTGGTGGTGCTGAATTGTTCATGAGACGTGCTTCTCTAAAGAAGGAAGAATTAGTCGTCCACCCAGCTAACTCTCCAATTGCCAACAAGAACCCAGACAACCCTAAGAAGACCACCACTTTGTCCTACGACGTTTACAAGGACAAGAGATTCTCCGAAGACCAATACGAATTGCACATTCCAATTGCTATCAACAAGTGTCCAAAGAACATCTTCAAGATCAACACTGAAGTCAGAGTTTTGTTAAAGCACGATGACAACCCTTACGTTATTGGTATCGACCGTGGTGAAAGAAATTTGTTGTACATTGTTGTTGTTGACGGTAAGGGTAACATCGTTGAACAATACTCCTTGAACGAAATCATCAACAACTTCAACGGTATTAGAATCAAGACTGATTACCACTCTTTGTTGGATAAGAAGGAAAAGGAACGTTTTGAAGCTCGTCAAAACTGGACCTCTATTGAAAACATCAAAGAATTGAAGGCTGGTTACATCAGTCAAGTTGTCCACAAGATCTGTGAATTGGTCGAGAAGTACGATGCCGTTATTGCCTTGGAAGATTTGAACTCTGGTTTTAAGAACTCTCGTGTCAAGGTTGAAAAGCAAGTCTACCAAAAGTTCGAAAAGATGTTAATCGACAAATTGAACTACATGGTTGACAAGAAATCCAACCCATGTGCTACCGGTGGTGCTTTGAAAGGTTACCAAATCACCAACAAATTCGAATCTTTCAAATCTATGTCCACTCAAAACGGGTTCATCTTCTACATTCCAGCTTGGTTGACCTCCAAGATCGACCCATCTACCGGTTTCGTTAACTTGTTGAAGACCAAGTACACTTCCATTGCTGATTCCAAGAAGTTCATCTCTTCTTTCGACAGAATCATGTACGTTCCAGAAGAAGACTTGTTCGAATTCGCCTTGGACTATAAGAACTTCTCCAGAACCGATGCTGACTACATTAAGAAATGGAAATTGTACTCCTACGGTAACAGAATCAGAATTTTCAGAAACCCAAAGAAAAACAACGTTTTCGATTGGGAAGAAGTTTGTTTGACTTCTGCCTACAAGGAATTATTCAACAAATACGGTATCAACTACCAACAAGGTGATATCAGAGCTTTGTTGTGTGAACAATCTGACAAGGCTTTCTACTCTTCCTTCATGGCTTTGATGTCCTTGATGTTGCAAATGAGAAACTCCATCACTGGTAGAACTGATGTCGACTTCCTCATTTCTCCAGTTAAGAATTCTGACGGTATTTTCTACGACTCTAGAAATTACGAAGCTCAAGAAAACGCTATTTTGCCAAAGAACGCTGATGCTAACGGTGCTTACAATATTGCTAGAAAGGTTTTGTGGGCTATCGGTCAATTCAAGAAGGCTGAAGACGAAAAGCTAGACAAGGTCAAGATTGCTATTTCTAACAAGGAATGGTTGGAATACGCTCAAACCTCCGTCAAGCACTCCAGAGCTGATCCAAAGAAGAAGAGAAAGGTATAAAGGAGTTAAAGGCAAAGTTTTCTTTTCTAGAGCCGTTCCCACAAATAATTATACGTATATGCTTCTTTTCGTTTACTATATATCTATATTTACAAGCCTTTATTCACTGATGCAATTTGTTTCCAAATACTTTTTTGGAGATCTCATAACTAGATATCATGATGGCGCAACTTGGCGCTATCTTAATTACTCTGGCTGCCAGGCCCGTGTAGAGGGCCGCAAGACCTTCTGTACGCCATATAGTCTCTAAGAACTTGAACAAGTTTCTAGACCTATTGCCGCCTTTCGGATCGCTATTGTTGCGGCCGCCAGCTGAAGCTTCGTACGCTGCAGGTCGACGAATTCTACCGTTCGTATAATGTATGCTATACGAAGTTATAGATCTGTTTAGCTTGCCTCGTCCCCGCCGGGTCACCCGGCCAGCGACATGGAGGCCCAGAATACCCTCCTTGACAGTCTTGACGTGCGCAGCTCAGGGGCATGATGTGACTGTCGCCCGTACATTTAGCCCATACATCCCCATGTATAATCATTTGCATCCATACATTTTGATGGCCGCACGGCGCGAAGCAAAAATTACGGCTCCTCGCTGCAGACCTGCGAGCAGGGAAACGCTCCCCTCACAGACGCGTTGAATTGTCCCCACGCCGCGCCCCTGTAGAGAAATATAAAAGGTTAGGATTTGCCACTGAGGTTCTTCTTTCATATACTTCCTTTTAAAATCTTGCTAGGATACAGTTCTCACATCACATCCGAACATAAACAACCATGGGTAAGGAAAAGACTCACGTTTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGCAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAGCTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATCAGTACTGACAATAAAAAGATTCTTGTTTTCAAGAACTTGTCATTTGTATAGTTTTTTTATATTGTAGTTGTTCTATTTTAATCAAATGTTAGCGTGATTTATATTTTTTTTCGCCTCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAATATCATGCGTCAATCGTATGTGAATGCTGGTCGCTATACTGCTGTCGATTCGATACTAACGCCGCCATCCAGTGTCGAAAACGAGCTCATAACTTCGTATAATGTATGCTATACGAACGGTAGAATTCGATATCAGATCCACTAGTGGCCTATGCGGCCGCCACCGCGGTGGAGCTCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGGGTCCTTTTCATCACGTGCTATAAAAATAATTATAATTTAAATTTTTTAATATAAATATATAAATTAAAAATAGAAAGTAAAAAAAGAAATTAAAGAAAAAATAGTTTTTGTTTTCCGAAGATGTAAAAGACTCTAGGGGGATCGCCAACAAATACTACCTTTTATCTTGCTCTTCCTGCTCTCAGGTATTAATGCCGAATTGTTTCATCTTGTCTGTGTAGAAGACCACACACGAAAATCCTGTGATTTTACATTTTACTTATCGTTAATCGAATGTATATCTATTTAATCTGCTTTTCTTGTCTAATAAATATATATGTAAAGTACGCTTTTTGTTGAAATTTTTTAAACCTTTGTTTATTTTTTTTTCTTCATTCCGTAACTCTTCTACCTTCTTTATTTACTTTCTAAAATCCAAATACAAAACATAAAAATAAATAAACACAGAGTAAATTCCCAAATTATTCCATCATTAAAAGATACGAGGCGCGTGTAAGTTACAGGCAAGCGATCCGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATAGATCACGACATTACTATATATATAATATAGGAAGCATTTAATAGAACAGCATCGTAATATATGTGTACTTTGCAGTTATGACGCCAGATGGCAGTAGTGGAAGATATTCTTTATTGAAAAATAGCTTGTCACCTTACGTACAATCTTGATCCGGAGCTTTTCTTTTTTTGCCGATTAAGAATTAATTCGGTCGAAAAAAGAAAAGGAGAGGGCCAAGAGGGAGGGCATTGGTGACTATTGAGCACGTGAGTATACGTGATTAAGCACACAAAGGCAGCTTGGAGTATGTCTGTTATTAATTTCACAGGTAGTTCTGGTCCATTGGTGAAAGTTTGCGGCTTGCAGAGCACAGAGGCCGCAGAATGTGCTCTAGATTCCGATGCTGACTTGCTGGGTATTATATGTGTGCCCAATAGAAAGAGAACAATTGACCCGGTTATTGCAAGGAAAATTTCAAGTCTTGTAAAAGCATATAAAAATAGTTCAGGCACTCCGAAATACTTGGTTGGCGTGTTTCGTAATCAACCTAAGGAGGATGTTTTGGCTCTGGTCAATGATTACGGCATTGATATCGTCCAACTGCATGGAGATGAGTCGTGGCAAGAATACCAAGAGTTCCTCGGTTTGCCAGTTATTAAAAGACTCGTATTTCCAAAAGACTGCAACATACTACTCAGTGCAGCTTCACAGAAACCTCATTCGTTTATTCCCTTGTTTGATTCAGAAGCAGGTGGGACAGGTGAACTTTTGGATTGGAACTCGATTTCTGACTGGGTTGGAAGGCAAGAGAGCCCCGAAAGCTTACATTTTATGTTAGCTGGTGGACTGACGCCAGAAAATGTTGGTGATGCGCTTAGATTAAATGGCGTTATTGGTGTTGATGTAAGCGGAGGTGTGGAGACAAATGGTGTAAAAGACTCTAACAAAATAGCAAATTTCGTCAAAAATGCTAAGAAATAGGTTATTACTGAGTAGTATTTATTTAAGTATTGTTTGTGCACTTGCCGATCTATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAGCGCGCGTAATACGACTCACTATAGGGCGAATTGGGTACCTTTTCTTTTTTTGCGGTCACCCCCATGTGGCGGGGA
质粒元件
CDS 965..4648
/codon_start=1
/product="CRISPR-associated protein from Lachnospiraceae
bacterium ND2006 (Zetsche et al., 2015)"
/translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
NTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
VKH"
SV40 NLS 4661..4681
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/translation="PKKKRKV"
lox66 complement(5030..5063)
/bound_moiety="Cre recombinase"
/note="loxP的右元件（RE）突变体（Araki等人，2010）。Cre介导的重组发生在8-bp核心序列（GCATACAT）中。"
gene 5109..6465
/note="赋予卡那霉素抗性的酵母选择性标记（Wach等人，1994）"
TEF promoter 5109..5452
/note="棉蚜TEF启动子"
KanR 5453..6262
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/note="赋予对卡那霉素的抗性"
/translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
terminator 6268..6465
/note="Ashbya棉蚜TEF终结者"
lox71 complement(6513..6546)
/bound_moiety="Cre recombinase"
/note="loxP的左元件（LE）突变体（Araki等人，2010）。Cre介导的重组发生在8-bp核心序列（GCATACAT）中。"
T3 promoter complement(6614..6632)
/note="噬菌体T3 RNA聚合酶启动子"
primer_bind complement(6653..6669)
/note="常见的测序引物，多种相似变体之一"
lac operator 6677..6693
/bound_moiety="lac repressor encoded by lacI"
/note="lac阻遏物与lac操作员结合，抑制大肠杆菌中的转录。这种抑制可以通过添加乳糖或异丙基β-D-硫代吡喃半乳糖苷（IPTG）来缓解。"
lac promoter complement(6701..6731)
/note="大肠杆菌lac操纵子的启动子"
CAP binding site 6746..6767
/bound_moiety="E. coli catabolite activator protein"
/note="CAP结合在cAMP存在下激活转录。"
ori complement(7055..7643)
/direction=LEFT
/note="高拷贝数ColE1/pMB1/pBR322/pUC复制起点"
AmpR complement(7814..8674)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="对氨苄青霉素、羧苄青霉素和相关抗生素产生耐药性"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
AmpR promoter complement(8675..8779)
/gene="bla"
CEN/ARS 8816..9319
/note="酿酒酵母CEN6着丝粒与自主复制序列融合"
TRP1 promoter 9578..9858
/gene="S. cerevisiae TRP1"
CDS 9859..10533
/codon_start=1
/gene="S. cerevisiae TRP1"
/product="phosphoribosylanthranilate isomerase, required
for tryptophan biosynthesis"
/note="酵母营养缺陷标志物"
/translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
KK"
f1 ori complement(10636..11091)
/direction=LEFT
/note="f1噬菌体复制起点；箭头表示（+）链合成的方向"
primer_bind 11232..11248
/note="常见的测序引物，多种相似变体之一"
T7 promoter 11258..11276
/note="噬菌体T7 RNA聚合酶启动子"
