pMonAID_nCas9-PmCDA_Hyg_ALS

产品货号 P6768

产品别称等效质粒Plasmid#91693

选择规格

少量干粉质粒（需转化提取）转染级质粒100μg（液体即用）转染级质粒200μg（液体即用）转染级质粒500μg（液体即用）

数　量

转染级质粒质检标准查看质粒手册

价格： ¥380.00

联系我们

基本信息
产品简介
产品声明
相关论文
产品文件
质粒元件

基本信息

产品简介

产品声明

For Research Use Only
All products available through the MiaoLing Plasmid Platform are intended for research use only. They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.
Plasmid Sequence and Experimental Performance
Plasmid products are provided and sold based on the corresponding reference/template plasmid. MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.
Sequence Verification of Certain Vector Products
For certain vector products, even the original manufacturer may not have fully verified the complete plasmid sequence. If the sequence of the key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.
After-Sales Period for Small-Pack Plasmid Products
The after-sales service period for small-pack plasmid products is three months from the date of receipt. Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.
Research-Related Risks and Limitation of Liability
Scientific research is inherently exploratory and involves a significant degree of uncertainty. To the maximum extent permitted by applicable law, MiaoLing shall not be liable for any additional economic losses, indirect losses, or other liabilities arising from the use of our products or from research outcomes.
Citation in Chinese Publications
For publications in Chinese, please cite the product as follows:
产品名称（产品货号）由淼灵质粒平台提供。
Citation in English Publications
For publications in English, please cite the product as follows:
“Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”

质粒序列

GATCCCGAGGGGAACCCTGTGGTTGGCATGCACATACAAATGGACGAACGGATAAACCTTTTCACGCCCTTTTAAATATCCGATTATTCTAATAAACGCTCTTTTCTCTTAGGTTTACCCGCCAATATATCCTGTCAAACACTGATAGTTTAAACTGAAGGCGGGAAACGACAATCTGATCCAAGCTCAAGCTCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACATGATTACGAATTTCAGGTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCGGCGCGTCCTGCAGGATAGGGATAACAGGGTAATGGCGCGCCAAGCTTATCGATACCGTCGACCTCGAGCGGCCGCGGGAATTCGATCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTCTTTTTTTCTTCTTCTTCGTTCATACAGTTTTTTTTTGTTTATCAGCTTACATTTTCTTGAACCGTAGCTTTCGTTTTCTTCTTTTTAACTTTCCATTCGGAGTTTTTGTATCTTGTTTCATAGTTTGTCCCAGGATTAGAATGATTAGGCATCGAACCTTCAAGAATTTGATTGAATAAAACATCTTCATTCTTAAGATATGAAGATAATCTTCAAAAGGCCCCTGGGAATCTGAAAGAAGAGAAGCAGGCCCATTTATATGGGAAAGAACAATAGTATTTCTTATATAGGCCCATTTAAGTTGAAAACAATCTTCAAAAGTCCCACATCGCTTAGATAAGAAAACGAAGCTGAGTTTATATACAGCTAGAGTCGAAGTAGTGATTGCGCGTCCATGGAGATCCACCGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTTCTAGACCCAGCTTTCTTGTACAAAGTTGGCATTAACCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGATCACTAGTGGATCCGAGCTCGGTACCTTAATTAAATTACCCTGTTATCCCTATCCTGCAGGATTAACACCCAACTTTTCTATCCAAGCTTGCCAACATGGTGGAGCACGACACTCTCGTCTACTCCAAGAATATCAAAGATACAGTCTCAGAAGACCAAAGGGCTATTGAGACTTTTCAACAAAGGGTAATATCGGGAAACCTCCTCGGATTCCATTGCCCAGCTATCTGTCACTTCATCAAAAGGACAGTAGAAAAGGAAGGTGGCACCTACAAATGCCATCATTGCGATAAAGGAAAGGCTATCGTTCAAGATGCCTCTGCCGACAGTGGTCCCAAAGATGGACCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGAACATGGTGGAGCACGACACTCTCGTCTACTCCAAGAATATCAAAGATACAGTCTCAGAAGACCAAAGGGCTATTGAGACTTTTCAACAAAGGGTAATATCGGGAAACCTCCTCGGATTCCATTGCCCAGCTATCTGTCACTTCATCAAAAGGACAGTAGAAAAGGAAGGTGGCACCTACAAATGCCATCATTGCGATAAAGGAAAGGCTATCGTTCAAGATGCCTCTGCCGACAGTGGTCCCAAAGATGGACCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGATATCTCCACTGACGTAAGGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGCCGGTCTAGAGAATTCCAAGCAACGAACTGCGAGTGATTCAAGAAAAAAGAAAACCTGAGCTTTCGATCTCTACGGAGTGGTTTCTTGTTCTTTGAAAAAGAGGGGGATTACATATGGCTCCTAAGAAGAAGCGGAAGGTTGGTATTCACGGGGTGCCTGCGGCTATGGACAAGAAGTACTCGATCGGGCTGGCCATCGGAACAAATTCTGTAGGCTGGGCTGTAATAACCGATGAGTACAAGGTGCCCTCTAAAAAATTTAAGGTCCTTGGCAATACGGATAGACATTCCATAAAGAAGAATCTTATCGGTGCGCTGCTCTTTGACAGCGGCGAGACCGCGGAGGCGACCCGGTTGAAACGCACCGCGAGACGCCGTTACACAAGGCGTAAGAATAGAATCTGTTATCTCCAGGAGATATTCTCTAATGAAATGGCGAAGGTAGACGATTCCTTCTTTCACCGTCTGGAGGAAAGTTTTCTCGTTGAGGAAGATAAGAAACATGAAAGACACCCGATCTTCGGAAACATTGTCGACGAGGTTGCTTATCATGAAAAGTACCCTACCATCTACCATCTTAGAAAGAAACTTGTTGACAGCACGGATAAGGCTGATCTCAGGCTGATATACCTGGCTCTGGCACATATGATTAAGTTCAGAGGGCATTTCCTTATCGAAGGCGACCTGAATCCAGATAATTCAGATGTAGACAAGCTCTTCATTCAACTTGTGCAGACTTATAATCAGCTCTTCGAAGAAAATCCAATAAACGCGTCGGGTGTAGACGCAAAGGCCATACTGTCCGCTAGGCTTTCTAAGTCACGTAGACTTGAGAATCTCATTGCCCAACTCCCCGGCGAGAAGAAGAACGGCTTGTTTGGAAATCTGATAGCGCTGTCCCTGGGTCTTACACCAAATTTCAAGAGTAATTTCGATTTGGCAGAAGATGCTAAGTTGCAGCTCAGTAAAGACACCTACGATGACGATCTTGATAATTTGTTGGCTCAGATTGGCGATCAGTATGCAGATCTTTTCTTGGCCGCTAAGAATTTGTCTGATGCAATTCTGCTTAGCGACATTTTGAGGGTTAATACAGAAATCACCAAGGCACCCTTGTCGGCGTCAATGATAAAGAGGTATGATGAGCACCACCAAGACCTGACGCTCCTCAAGGCTCTTGTTCGGCAGCAATTGCCGGAGAAGTACAAAGAGATCTTCTTCGACCAGTCTAAGAACGGATATGCGGGCTACATAGACGGTGGAGCGAGTCAGGAGGAATTCTACAAGTTCATAAAGCCCATTCTCGAGAAGATGGATGGTACGGAAGAACTGCTTGTGAAACTTAACAGAGAAGATCTTTTGCGGAAGCAGAGAACTTTCGACAACGGAAGTATACCACACCAGATACATCTCGGAGAGCTTCATGCTATTCTCAGAAGACAAGAGGATTTCTACCCTTTCTTGAAGGATAACAGAGAAAAGATAGAGAAGATCCTCACGTTTAGGATCCCTTACTACGTAGGTCCTCTTGCTCGCGGCAATAGTAGGTTCGCCTGGATGACCCGCAAGTCTGAAGAAACTATCACCCCTTGGAATTTCGAAGAGGTTGTAGACAAAGGTGCTTCAGCACAGAGTTTCATTGAGAGGATGACCAACTTCGACAAGAACCTCCCCAACGAAAAGGTCCTGCCTAAGCACAGCCTCCTCTACGAATACTTTACTGTCTATAATGAGCTTACAAAAGTTAAGTACGTGACAGAGGGAATGCGGAAGCCCGCATTCCTTTCCGGAGAACAAAAGAAGGCGATCGTGGATCTTCTCTTCAAGACGAACCGCAAGGTGACGGTTAAACAGTTGAAGGAAGATTACTTCAAGAAGATAGAATGTTTTGATAGCGTGGAAATCAGCGGCGTCGAAGATAGGTTCAACGCTTCCCTGGGAACGTACCACGATCTCCTCAAGATTATCAAAGATAAGGACTTTCTTGATAACGAAGAGAATGAGGACATCTTGGAAGACATTGTTCTGACGCTCACCCTGTTCGAAGATCGCGAGATGATTGAGGAACGCTTGAAGACCTACGCACACCTGTTCGATGACAAGGTTATGAAGCAACTTAAACGGCGCCGGTATACGGGCTGGGGACGGCTTTCGCGGAAGCTGATAAATGGAATCCGTGACAAGCAGTCTGGCAAGACAATACTCGACTTCTTGAAGTCGGATGGTTTTGCCAATAGAAATTTTATGCAACTCATTCATGATGACTCGCTTACTTTTAAGGAGGACATCCAGAAGGCCCAGGTATCAGGACAGGGTGACTCTTTGCACGAACACATCGCGAACCTGGCGGGCTCCCCCGCGATTAAGAAGGGAATTTTGCAGACTGTCAAGGTGGTCGATGAACTCGTGAAGGTTATGGGACGTCATAAGCCGGAAAATATTGTGATTGAGATGGCTCGCGAGAATCAAACAACACAGAAGGGCCAAAAGAACAGTAGAGAACGCATGAAGCGCATCGAAGAGGGCATCAAAGAGCTGGGCAGTCAGATCCTTAAAGAACATCCAGTCGAGAATACACAGCTTCAGAACGAAAAGCTGTACCTTTATTACCTTCAAAATGGGCGTGATATGTATGTGGATCAGGAACTCGATATCAATAGGCTGAGTGACTATGATGTCGACCATATCGTCCCGCAAAGTTTCCTCAAGGACGACAGTATAGACAACAAAGTTCTCACACGGTCAGATAAGAATCGCGGCAAGAGCGATAATGTACCGTCGGAGGAGGTAGTCAAGAAGATGAAGAATTACTGGCGCCAGTTGCTCAACGCCAAGCTCATCACTCAGAGGAAATTTGACAACCTTACGAAAGCCGAGCGGGGCGGACTCTCTGAACTGGACAAGGCCGGTTTCATAAAGCGCCAGCTCGTTGAGACACGTCAAATTACTAAGCACGTCGCTCAAATATTGGATTCCCGCATGAATACTAAGTACGATGAGAATGATAAGCTCATACGTGAAGTTAAGGTCATTACTCTCAAGTCCAAGCTTGTATCGGACTTCCGTAAGGACTTCCAATTCTACAAGGTCCGGGAAATCAATAATTATCACCATGCCCATGACGCTTATCTGAACGCGGTCGTGGGCACGGCACTCATTAAGAAATACCCAAAACTTGAGTCAGAATTTGTTTACGGGGACTATAAAGTTTATGACGTGCGGAAGATGATAGCGAAGTCGGAACAAGAGATAGGAAAGGCGACTGCAAAGTACTTTTTTTACTCCAACATAATGAATTTCTTTAAGACCGAAATAACCCTTGCAAACGGTGAAATCAGAAAGCGGCCTCTGATTGAAACAAATGGCGAGACGGGCGAGATCGTCTGGGACAAGGGGAGGGACTTCGCAACGGTTCGCAAGGTCCTTAGCATGCCGCAAGTAAATATAGTTAAGAAGACGGAAGTTCAGACCGGCGGCTTTAGTAAAGAAAGCATACTTCCTAAAAGGAATTCCGACAAACTGATAGCGCGCAAGAAGGACTGGGATCCAAAGAAGTATGGAGGATTTGACTCCCCAACCGTTGCTTATAGCGTGTTGGTAGTAGCCAAGGTGGAAAAGGGTAAGTCTAAGAAATTGAAGTCGGTGAAGGAGTTGTTGGGGATAACTATAATGGAGCGGAGTTCGTTCGAGAAGAACCCAATTGACTTTCTCGAAGCCAAAGGCTACAAGGAGGTCAAGAAGGACCTGATTATTAAGTTGCCAAAGTACTCGCTCTTCGAACTCGAGAACGGGAGAAAGCGTATGCTGGCGTCGGCGGGCGAGCTGCAGAAAGGAAACGAGCTGGCTTTGCCATCGAAATACGTAAATTTCCTGTACCTCGCCTCACATTATGAGAAGCTTAAAGGGTCTCCAGAAGACAATGAACAGAAGCAGCTGTTTGTTGAACAGCACAAGCACTACTTGGACGAGATTATAGAACAAATCTCCGAGTTCTCTAAACGGGTTATCCTTGCAGACGCCAATTTGGATAAGGTCCTCTCGGCTTATAATAAGCATAGAGATAAGCCAATCCGGGAGCAGGCTGAAAATATCATACACCTCTTTACGTTGACTAATTTGGGTGCGCCAGCGGCATTCAAGTACTTCGATACAACAATCGATCGTAAGCGCTACACAAGCACTAAGGAAGTCCTGGACGCGACGCTGATACACCAGTCCATTACTGGACTGTATGAAACCAGAATAGATCTTAGCCAGCTCGGCGGTGATGGAGGAGGGCCTAGGGCTGATCCTAAGAAGAAGAGGAAGGTTGGAGGAGGGCCAGGAGCAGAGTATGTTAGAGCGTTGTTTGATTTTAACGGTAACGATGAAGAGGATTTACCCTTTAAGAAAGGCGACATTCTCAGGATTAGGGATAAACCTGAAGAGCAATGGTGGAATGCTGAGGATAGTGAAGGCAAACGAGGAATGATTTTAGTGCCGTATGTGGAGAAATATTCGGGTGACTACAAAGATCATGATGGTGATTACAAAGACCATGACATCGACTACAAGGATGATGATGATAAGTCAGGGATGACAGATGCTGAATATGTCAGAATCCACGAAAAGTTGGACATTTACACGTTTAAGAAGCAGTTCTTCAACAACAAGAAATCTGTTTCGCATAGGTGCTATGTGCTTTTCGAACTAAAACGTCGTGGAGAAAGACGGGCTTGCTTTTGGGGTTACGCGGTTAACAAACCACAATCAGGTACTGAACGAGGAATACACGCTGAAATCTTTTCTATCCGAAAGGTTGAGGAATATCTACGTGACAATCCTGGACAGTTCACTATCAATTGGTATTCTAGCTGGTCACCATGTGCAGATTGTGCTGAGAAGATTCTCGAATGGTACAATCAAGAGCTTAGAGGCAATGGACATACATTGAAAATATGGGCATGCAAGCTCTACTACGAAAAGAATGCCAGAAACCAAATTGGGCTTTGGAACTTGAGGGATAATGGAGTTGGGCTTAATGTCATGGTTTCTGAGCACTATCAATGTTGTCGGAAGATCTTCATACAAAGTTCCCATAACCAGTTGAATGAGAACAGATGGTTAGAGAAAACCCTTAAAAGAGCCGAGAAGAGAAGATCCGAACTGAGCATTATGATACAGGTCAAAATTCTGCATACCACTAAGAGTCCAGCTGTAGGTCCTAAGAAGAAACGTAAAGTAGGGCCCTGATCCAGGCCTCCCAGCTTTCGTCCGTATCATCGGTTTCGACAACGTTCGTCAAGTTCAATGCATCAGTTTCATTGCCCACACACCAGAATCCTACTAAGTTTGAGTATTATGGCATTGGAAAAGCTGTTTTCTTCTATCATTTGTTCTGCTTGTAATTTACTGTGTTCTTTCAGTTTTTGTTTTCGGACATCAAAATGCAAATGGATGGATAAGAGTTAATAAATGATATGGTCCTTTTGTTCATTCTCAAATTATTATTATCTGTTGTTTTTACTTTAATGGGTTGAATTTAAGTAAGAAAGGAACTAACAGTGTGATATTAAGGTGCAATGTTAGACATATAAAACAGTCTTTCACCTCTCTTTGGTTATGTCTTGAATTGGTTTGTTTCTTCACTTATCTGTGTAATCAAGTTTACTATGAGTCTATGATCAAGTAATTATGCAATCAAGTTAAGTACAGTATAGGCTTGAGCTCCCTAGGCCCCTAGGTAATTCTTCGGACCCAAGAATGCTAAGCCAAGAGGAGCTGTTATCGCCGTCCTCCTGCTTGTTTCTCTCTTTTTGTTGCTGTTTCTTCATTAGCGTGGACAAAGTTTTCAACCGGCCTATCTGTTATCATTTTCTTCTATTCAAAGACTGTAATACCTATTGCTACCTGTGGTTCTCACTTGTGATTTTGGACACATATGTTCGGTTTATTCAAATTTAATCAGATGCCTGATGAGGGTACCAGAAAAAATACGTGTTCTGGTTGTTTTTGAGTTGCGATTATTCTATGAAATGAATAACATCGAAGTTATCATCCCAGTATTTTCGCATGAATGTTCTTTTCTTCTGTCTTGTGCATCAGTGATCTAGTGCATGGGAGTTTGTATTGTGATGTTCGACATCACGTAACTTCCACTTTGCCTTTGCTGTTCGATATTTTAATGACATGTCACACACACTTCTGATACTTTTCTTTCTTGGCTATTGTGCCAGCATGATGCAAGATGCATCACAGCATCAGATATATTCTCATCGTCAGGCTTTAGCAGCACACGAGCACGCTTTGCCGCTTAAAAGTTGTACGGCGCAGCTTAGACATCCCCTGTAGAAGTGATAATCTTTTCACTTTTCCTTAAACAAATTGAGAGGGGAAATGGAACCATGTGGATCAGAGAAGCTTTTGTTTCTTTACACAAGAATATTTGGTACAGTGGGGGTCCTATGTTCGTGGGTTCGTGGCTTGGCTGCCTGTCTTCAACCAAGTGTTTTCAGTTCAACATGTTAGCGTGTAGAAAGAGCACAATTCTGTTTATCTCCAAGGTAAAATGTGGCATTCTGTTAAAGAACATGATCCTGCCAATTTTTTAAGTTTCAATGGAAGAGGAATGTAAAGCTTTCTATGGTTTGTGTACACAACACAGTGGAAGAGGAGTGCAAGCTTTCTATGGTTTGTGTGCGCGTTGTGTGTCAGCACTTCAATTTTGTTAGAAAATGAAAGAAAAAAAAGGATGATCATGCTTATAGTAAATCACTCTTTTTCCTCGCCTTCTGTACGTTTTGACTTGACAAGATTTTAAAATCTGTACATGACCTTTGTTTTAAAATTACTTTATGTATTTCCATCTTTCAAGTTATGCAGATGTCATCACAAATTGTTACACCAATCACCAGGCTGGCTGTTTATATATTATCAGACCAGGCTATATAGAGTATACTATACTAACTGTTCATATTATCTGGAAATCTTGCTTGCTACTTGAGCGGTAAAAGGGTATAGATATGAGGGTCCCCAGATTAGCCTTTTCAATTTCAGAAAGAATGCTAACCCACAGATGGTTAGAGAGGCTTACGCAGCAGGTCTCATCAAGACGATCTACCCGAGCAATAATCTCCAGGAAATCAAATACCTTCCCAAGAAGGTTAAAGATGCAGTCAAAAGATTCAGGACTAACTGCATCAAGAACACAGAGAAAGATATATTTCTCAAGATCAGAAGTACTATTCCAGTATGGACGATTCAAGGCTTGCTTCACAAACCAAGGCAAGTAATAGAGATTGGAGTCTCTAAAAAGGTAGTTCCCACTGAATCAAAGGCCATGGAGTCAAAGATTCAAATAGAGGACCTAACAGAACTCGCCGTAAAGACTGGCGAACAGTTCATACAGAGTCTCTTACGACTCAATGACAAGAAGAAAATCTTCGTCAACATGGTGGAGCACGACACACTTGTCTACTCCAAAAATATCAAAGATACAGTCTCAGAAGACCAAAGGGCAATTGAGACTTTTCAACAAAGGGTAATATCCGGAAACCTCCTCGGATTCCATTGCCCAGCTATCTGTCACTTTATTGTGAAGATAGTGGAAAAGGAAGGTGGCTCCTACAAATGCCATCATTGCGATAAAGGAAAGGCCATCGTTGAAGATGCCTCTGCCGACAGTGGTCCCAAAGATGGACCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGATATCTCCACTGACGTAAGGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGAACACGGGGGACTCTAGAATGAAAAAGCCTGAACTCACCGCGACGTCTGTCGAGAAGTTTCTGATCGAAAAGTTCGACAGCGTCTCCGACCTGATGCAGCTCTCGGAGGGCGAAGAATCTCGTGCTTTCAGCTTCGATGTAGGAGGGCGTGGATATGTCCTGCGGGTAAATAGCTGCGCCGATGGTTTCTACAAAGATCGTTATGTTTATCGGCACTTTGCATCGGCCGCGCTCCCGATTCCGGAAGTGCTTGACATTGGGGAGTTTAGCGAGAGCCTGACCTATTGCATCTCCCGCCGTTCACAGGGTGTCACGTTGCAAGACCTGCCTGAAACCGAACTGCCCGCTGTTCTACAACCGGTCGCGGAGGCTATGGATGCGATCGCTGCGGCCGATCTTAGCCAGACGAGCGGGTTCGGCCCATTCGGACCGCAAGGAATCGGTCAATACACTACATGGCGTGATTTCATATGCGCGATTGCTGATCCCCATGTGTATCACTGGCAAACTGTGATGGACGACACCGTCAGTGCGTCCGTCGCGCAGGCTCTCGATGAGCTGATGCTTTGGGCCGAGGACTGCCCCGAAGTCCGGCACCTCGTGCACGCGGATTTCGGCTCCAACAATGTCCTGACGGACAATGGCCGCATAACAGCGGTCATTGACTGGAGCGAGGCGATGTTCGGGGATTCCCAATACGAGGTCGCCAACATCTTCTTCTGGAGGCCGTGGTTGGCTTGTATGGAGCAGCAGACGCGCTACTTCGAGCGGAGGCATCCGGAGCTTGCAGGATCGCCACGACTCCGGGCGTATATGCTCCGCATTGGTCTTGACCAACTCTATCAGAGCTTGGTTGACGGCAATTTCGATGATGCAGCTTGGGCGCAGGGTCGATGCGACGCAATCGTCCGATCCGGAGCCGGGACTGTCGGGCGTACACAAATCGCCCGCAGAAGCGCGGCCGTCTGGACCGATGGCTGTGTAGAAGTACTCGCCGATAGTGGAAACCGACGCCCCAGCACTCGTCCGAGGGCAAAGAAATAGGAGCTCTGGGCTAATCTAAAACGATTTATCTGTGGCTTCAAGTGTATCGATCACTTATGTGAGGTGTAATTACTGGTGTTTTTGGTGTGCTCTGGTTCCTTTCAAGTGTGTTGTTGCCGCTCGAACTACTCCGCTATGTAAAACGGTAAAACCTGTTGTCTCATTATGAAAGTGAACTATATTATGTTCTACTACTACTCTACTTAGTCAATTTTCTTCACCTTGATTAGTGTAAATATGAATCTTATATTCTTATGTCTTAAGAAATTAGCACATGTGAAGCCTCCAAGTGCATATTTTCTCGATCGCGAGACGCACAATGCGTGAGAAATTCAGCTGGTTATACTCAAATATATTAATATATCTAGCAGCAGCTCATGGAGATTCAGGAAACTTGGCATCCCTAATCCCTACCATTTCCATTCTTCCGAGATTGACAGTTCAATACAAGTACAGTAATCTCCTGGTAAGTTTCTTATTAACTTGACATGTAGTAGTAATAATTTGTACGTAGCATAGATACATAGACACAAAAATGTCCTCCCCATTGAGCTAGCCGATTGGAGCCGAACACCGCAGGAATGAATTTACATAATCTGCAAAGAATAAATGGAATGTGCCTCCACAGGAAAACCAGCGGCAGTGTGGCGTTTTCAAGAGCAGCCGTAAGTCGAAGCCTATTCTGAATCGTAGAAATCACTGGGGCATGGTGTAATTACATCCGACTCCAACATCTGTACCACCCTGTGCATTGTAGGCCGCTCCTCTGGCAAAGAACTTACACATTGTTTAGCAAGAGAAAGTAGAGCATCCAAGGTCTCAATCTGCACTCCCTCACAATATGGATCGACAATTTCCCTCTCCCGATTCTCACCGACCAGGAAATTCAACTGCCACAGAGCAAGTAGATTATTTCAAGAATACATTAAATCAATTGAAGGCATACGTAATTCATATCAGAAAACTGTGGATATGAAATGGAATGACATAAAGGTCATACATACCCATCCAACAATGTTCAATCCCTTTTCAATAAATGATGCATCAGTAGGTCGTTTTCCGCTTAGTATTTCAAGTAGCAAAACTCCAAAACTGTAGACGTCAGTCTTTTCGGTGGCTCTGCCACTTTGCATATACTCCTGCAGGTCGACCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGTGATATCCCGCGGAAATACGTAGAATTCGGGAAGCTTGGCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGCTAGAGCAATTCGGCGTTAATTCAGTACATTAAAAACGTCCGCAATGTGTTATTAAGTTGTCTAAGCGTCAATTTGTTTACACCACAATATATCCTGCCACCAGCCAGCCAACAGCTCCCCGACCGGCAGCTCGGCACAAAATCACCACTCGATACAGGCAGCCCATCAGTCCGGGACGGCGTCAGCGGGAGAGCCGTTGTAAGGCGGCAGACTTTGCTCATGTTACCGATGCTATTCGGAAGAACGGCAACTAAGCTGCCGGGTTTGAAACACGGATGATCTCGCGGAGGGTAGCATGTTGATTGTAACGATGACAGAGCGTTGCTGCCTGTGATCAATTCGGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGGGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAATGTCTAGCTAGAAATTCGTTCAAGCCGACGCCGCTTCGCGGCGCGGCTTAACTCAAGCGTTAGATGCACTAAGCACATAATTGCTCACAGCCAAACTATCAGGTCAAGTCTGCTTTTATTATTTTTAAGCGTGCATAATAAGCCCTACACAAATTGGGAGATATATCATGCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGGGTGCCTTGATGTGGGCGCCGGCGGTCGAGTGGCGACGGCGCGGCTTGTCCGCGCCCTGGTAGATTGCCTGGCCGTAGGCCAGCCATTTTTGAGCGGCCAGCGGCCGCGATAGGCCGACGCGAAGCGGCGGGGCGTAGGGAGCGCAGCGACCGAAGGGTAGGCGCTTTTTGCAGCTCTTCGGCTGTGCGCTGGCCAGACAGTTATGCACAGGCCAGGCGGGTTTTAAGAGTTTTAATAAGTTTTAAAGAGTTTTAGGCGGAAAAATCGCCTTTTTTCTCTTTTATATCAGTCACTTACATGTGTGACCGGTTCCCAATGTACGGCTTTGGGTTCCCAATGTACGGGTTCCGGTTCCCAATGTACGGCTTTGGGTTCCCAATGTACGTGCTATCCACAGGAAAGAGACCTTTTCGACCTTTTTCCCCTGCTAGGGCAATTTGCCCTAGCATCTGCTCCGTACATTAGGAACCGGCGGATGCTTCGCCCTCGATCAGGTTGCGGTAGCGCATGACTAGGATCGGGCCAGCCTGCCCCGCCTCCTCCTTCAAATCGTACTCCGGCAGGTCATTTGACCCGATCAGCTTGCGCACGGTGAAACAGAACTTCTTGAACTCTCCGGCGCTGCCACTGCGTTCGTAGATCGTCTTGAACAACCATCTGGCTTCTGCCTTGCCTGCGGCGCGGCGTGCCAGGCGGTAGAGAAAACGGCCGATGCCGGGATCGATCAAAAAGTAATCGGGGTGAACCGTCAGCACGTCCGGGTTCTTGCCTTCTGTGATCTCGCGGTACATCCAATCAGCTAGCTCGATCTCGATGTACTCCGGCCGCCCGGTTTCGCTCTTTACGATCTTGTAGCGGCTAATCAAGGCTTCACCCTCGGATACCGTCACCAGGCGGCCGTTCTTGGCCTTCTTCGTACGCTGCATGGCAACGTGCGTGGTGTTTAACCGAATGCAGGTTTCTACCAGGTCGTCTTTCTGCTTTCCGCCATCGGCTCGCCGGCAGAACTTGAGTACGTCCGCAACGTGTGGACGGAACACGCGGCCGGGCTTGTCTCCCTTCCCTTCCCGGTATCGGTTCATGGATTCGGTTAGATGGGAAACCGCCATCAGTACCAGGTCGTAATCCCACACACTGGCCATGCCGGCCGGCCCTGCGGAAACCTCTACGTGCCCGTCTGGAAGCTCGTAGCGGATCACCTCGCCAGCTCGTCGGTCACGCTTCGACAGACGGAAAACGGCCACGTCCATGATGCTGCGACTATCGCGGGTGCCCACGTCATAGAGCATCGGAACGAAAAAATCTGGTTGCTCGTCGCCCTTGGGCGGCTTCCTAATCGACGGCGCACCGGCTGCCGGCGGTTGCCGGGATTCTTTGCGGATTCGATCAGCGGCCGCTTGCCACGATTCACCGGGGCGTGCTTCTGCCTCGATGCGTTGCCGCTGGGCGGCCTGCGCGGCCTTCAACTTCTCCACCAGGTCATCACCCAGCGCCGCGCCGATTTGTACCGGGCCGGATGGTTTGCGACCGCTCACGCCGATTCCTCGGGCTTGGGGGTTCCAGTGCCATTGCAGGGCCGGCAGACAACCCAGCCGCTTACGCCTGGCCAACCGCCCGTTCCTCCACACATGGGGCATTCCACGGCGTCGGTGCCTGGTTGTTCTTGATTTTCCATGCCGCCTCCTTTAGCCGCTAAAATTCATCTACTCATTTATTCATTTGCTCATTTACTCTGGTAGCTGCGCGATGTATTCAGATAGCAGCTCGGTAATGGTCTTGCCTTGGCGTACCGCGTACATCTTCAGCTTGGTGTGATCCTCCGCCGGCAACTGAAAGTTGACCCGCTTCATGGCTGGCGTGTCTGCCAGGCTGGCCAACGTTGCAGCCTTGCTGCTGCGTGCGCTCGGACGGCCGGCACTTAGCGTGTTTGTGCTTTTGCTCATTTTCTCTTTACCTCATTAACTCAAATGAGTTTTGATTTAATTTCAGCGGCCAGCGCCTGGACCTCGCGGGCAGCGTCGCCCTCGGGTTCTGATTCAAGAACGGTTGTGCCGGCGGCGGCAGTGCCTGGGTAGCTCACGCGCTGCGTGATACGGGACTCAAGAATGGGCAGCTCGTACCCGGCCAGCGCCTCGGCAACCTCACCGCCGATGCGCGTGCCTTTGATCGCCCGCGACACGACAAAGGCCGCTTGTAGCCTTCCATCCGTGACCTCAATGCGCTGCTTAACCAGCTCCACCAGGTCGGCGGTGGCCCATATGTCGTAAGGGCTTGGCTGCACCGGAATCAGCACGAAGTCGGCTGCCTTGATCGCGGACACAGCCAAGTCCGCCGCCTGGGGCGCTCCGTCGATCACTACGAAGTCGCGCCGGCCGATGGCCTTCACGTCGCGGTCAATCGTCGGGCGGTCGATGCCGACAACGGTTAGCGGTTGATCTTCCCGCACGGCCGCCCAATCGCGGGCACTGCCCTGGGGATCGGAATCGACTAACAGAACATCGGCCCCGGCGAGTTGCAGGGCGCGGGCTAGATGGGTTGCGATGGTCGTCTTGCCTGACCCGCCTTTCTGGTTAAGTACAGCGATAACCTTCATGCGTTCCCCTTGCGTATTTGTTTATTTACTCATCGCATCATATACGCAGCGACCGCATGACGCAAGCTGTTTTACTCAAATACACATCACCTTTTTAGACGGCGGCGCTCGGTTTCTTCAGCGGCCAAGCTGGCCGGCCAGGCCGCCAGCTTGGCATCAGACAAACCGGCCAGGATTTCATGCAGCCGCACGGTTGAGACGTGCGCGGGCGGCTCGAACACGTACCCGGCCGCGATCATCTCCGCCTCGATCTCTTCGGTAATGAAAAACGGTTCGTCCTGGCCGTCCTGGTGCGGTTTCATGCTTGTTCCTCTTGGCGTTCATTCTCGGCGGCCGCCAGGGCGTCGGCCTCGGTCAATGCGTCCTCACGGAAGGCACCGCGCCGCCTGGCCTCGGTGGGCGTCACTTCCTCGCTGCGCTCAAGTGCGCGGTACAGGGTCGAGCGATGCACGCCAAGCAGTGCAGCCGCCTCTTTCACGGTGCGGCCTTCCTGGTCGATCAGCTCGCGGGCGTGCGCGATCTGTGCCGGGGTGAGGGTAGGGCGGGGGCCAAACTTCACGCCTCGGGCCTTGGCGGCCTCGCGCCCGCTCCGGGTGCGGTCGATGATTAGGGAACGCTCGAACTCGGCAATGCCGGCGAACACGGTCAACACCATGCGGCCGGCCGGCGTGGTGGTGTCGGCCCACGGCTCTGCCAGGCTACGCAGGCCCGCGCCGGCCTCCTGGATGCGCTCGGCAATGTCCAGTAGGTCGCGGGTGCTGCGGGCCAGGCGGTCTAGCCTGGTCACTGTCACAACGTCGCCAGGGCGTAGGTGGTCAAGCATCCTGGCCAGCTCCGGGCGGTCGCGCCTGGTGCCGGTGATCTTCTCGGAAAACAGCTTGGTGCAGCCGGCCGCGTGCAGTTCGGCCCGTTGGTTGGTCAAGTCCTGGTCGTCGGTGCTGACGCGGGCATAGCCCAGCAGGCCAGCGGCGGCGCTCTTGTTCATGGCGTAATGTCTCCGGTTCTAGTCGCAAGTATTCTACTTTATGCGACTAAAACACGCGACAAGAAAACGCCAGGAAAAGGGCAGGGCGGCAGCCTGTCGCGTAACTTAGGACTTGTGCGACATGTCGTTTTCAGAAGACGGCTGCACTGAACGTCAGAAGCCGACTGCACTATAGCAGCGGAGGGGTTGGATCAAAGTACTTT

产品文件

P6768_全序列图谱.dna

质粒元件

                                                            
     RB T-DNA repeat    113..137
                     /note="胭脂碱C58 T-DNA的右边界重复序列"
     CAP binding site    295..316
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP结合在cAMP存在下激活转录。"
     lac promoter        331..361
                     /note="大肠杆菌lac操纵子的启动子"
                     /note="此正向特征有3个分段：1:331。.336/#ffff/-35 2:337。.354/#fffff 3:355。.361/#ffff/-10"
     lac operator    369..385
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac阻遏物与lac操作员结合，抑制大肠杆菌中的转录。这种抑制可以通过添加乳糖或异丙基β-D-硫代吡喃半乳糖苷（IPTG）来缓解。"
     primer_bind     393..409
                     /note="常见的测序引物，多种相似变体之一"
     attB1    485..509
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /note="Gateway（R）BP反应的重组位点"
     primer_bind     complement(559..575)
                     /note="常见的测序引物，多种相似变体之一"
     attL1    596..695
                     /gene="mutant version of attL"
                     /bound_moiety="LR Clonase(TM)"
                     /note="Gateway（R）LR反应的重组位点"
     misc_RNA        1104..1179
                     /note="化脓性链球菌CRISPR/Cas9系统的RNA支架"
     attB2    complement(1191..1215)
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /note="Gateway（R）BP反应的重组位点"
     attL2    complement(1221..1320)
                     /gene="mutant version of attL"
                     /bound_moiety="LR Clonase(TM)"
                     /note="Gateway（R）LR反应的重组位点"
     OsADH 5'-UTR        2111..2156
                     /note="花椰菜花叶病毒最小35S启动子"
     5'UTR           2167..2267
                     /note="水稻醇脱氢酶基因5'-UTR的翻译增强子（Sugio等人，2008）"
     SV40 NLS             2277..2297
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             2322..6425
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /note="由于RuvC催化结构域中的D10A突变，在DNA中产生RNA引导的单链缺口"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     SV40 NLS             6447..6467
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             6660..6725
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     Cleavage site after base 6725"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6732..7355
                     /codon_start=1
                     /product="cytidine deaminase from the sea lamprey 
                     Petromyzon marinus (Rogozin et al., 2007)"
                     /translation="MTDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGER
                     RACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAEKI
                     LEWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCRKIF
                     IQSSHNQLNENRWLEKTLKRAEKRRSELSIMIQVKILHTTKSPAV"
     SV40 NLS             7359..7379
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CaMV 35S promoter        9657..10002
                     /note="花椰菜花叶病毒强组成型启动子"
     HygR             10017..11042
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /note="赋予对潮霉素的抗性"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     attR2    complement(12194..12318)
                     /gene="mutant version of attR"
                     /bound_moiety="LR Clonase(TM)"
                     /note="Gateway（R）LR反应的重组位点"
     primer_bind     complement(12358..12374)
                     /note="常见的测序引物，多种相似变体之一"
     LB T-DNA repeat    12589..12613
                     /note="胭脂碱C58 T-DNA的左边界重复序列"
     SmR             13134..13925
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /note="赋予对壮观霉素和链霉素的抗性"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     ori      14171..14759
                     /direction=RIGHT
                     /note="高拷贝数ColE1/pMB1/pBR322/pUC复制起点"
     bom    14945..15085
                     /note="pBR322迁移区的基础"
     pVS1 oriV      15429..15623
                     /note="假单胞菌质粒pVS1的复制起点（Heeb等人，2000）"
     CDS             complement(15689..16762)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(17191..17820)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"                             
                        

pMonAID_nCas9-PmCDA_Hyg_ALS

基本信息

产品简介

产品声明

质粒序列

相关论文

产品文件

质粒元件

质粒序列