pJK-caCas9-NatMX-Neut5L-Ade2 drive
产品声明
For Research Use Only research use only . They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.Plasmid Sequence and Experimental Performance reference/template plasmid . MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.Sequence Verification of Certain Vector Products key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.After-Sales Period for Small-Pack Plasmid Products three months from the date of receipt . Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.Research-Related Risks and Limitation of Liability Citation in Chinese Publications 产品名称（产品货号）由淼灵质粒平台提供。 Citation in English Publications “Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”
质粒序列
一键复制序列（有部分质粒是非全长序列）
ATGGATAAAAAATACTCCATAGGTTTGGACATTGGAACCAATTCAGTTGGATGGGCTGTAATCACTGATGAATATAAGGTTCCTTCTAAAAAGTTCAAAGTTTTAGGAAATACTGATAGACACTCCATTAAAAAGAATTTAATTGGTGCCTTGTTGTTTGACAGTGGTGAAACAGCTGAAGCAACTCGTTTGAAAAGAACAGCTAGAAGAAGATATACCAGAAGAAAGAACAGAATTTGTTACTTGCAAGAAATCTTTTCTAATGAAATGGCAAAGGTTGATGATTCTTTTTTTCATAGATTGGAAGAAAGTTTCTTAGTTGAAGAAGATAAGAAACATGAAAGACATCCAATCTTTGGTAATATTGTAGATGAAGTGGCTTACCACGAAAAATATCCTACAATTTATCATTTGAGAAAAAAATTAGTTGACTCCACTGATAAAGCTGACTTAAGACTTATATATTTGGCATTGGCTCACATGATCAAGTTCAGAGGTCACTTCTTAATTGAAGGTGATTTGAATCCAGATAATTCCGATGTCGACAAGTTGTTTATTCAATTGGTTCAAACTTACAACCAACTTTTCGAGGAAAATCCAATCAATGCCTCAGGTGTGGATGCTAAAGCTATTTTATCTGCTAGATTGTCAAAAAGTAGACGTTTGGAAAATCTTATTGCCCAATTGCCAGGTGAAAAAAAAAATGGATTGTTTGGAAACTTAATTGCATTATCTTTGGGTTTGACCCCAAATTTCAAATCAAACTTTGATTTGGCCGAGGATGCTAAACTTCAATTGTCTAAGGATACATACGATGACGATTTAGATAATCTTCTTGCCCAAATTGGTGATCAATATGCAGATTTATTCTTGGCAGCTAAGAACTTGAGTGATGCAATTTTGTTAAGTGACATTTTGAGAGTCAACACAGAAATTACAAAAGCTCCTTTATCCGCCTCTATGATTAAAAGATATGATGAACACCATCAAGATTTAACCTTGTTGAAAGCTCTTGTACGTCAACAATTACCAGAAAAGTATAAAGAAATATTCTTTGATCAATCTAAAAATGGTTACGCTGGTTATATTGATGGTGGTGCTAGTCAAGAAGAATTTTATAAGTTTATCAAGCCAATTCTTGAAAAAATGGATGGTACTGAGGAATTGTTGGTTAAGTTGAATCGAGAGGATTTACTTCGTAAGCAAAGAACATTTGATAACGGTTCTATCCCTCATCAAATCCACTTAGGTGAGTTACACGCTATCTTGAGACGTCAGGAAGATTTTTACCCTTTTTTGAAAGACAATCGTGAAAAAATTGAAAAAATTTTGACTTTCAGAATTCCTTATTACGTCGGGCCATTGGCTAGAGGTAATTCTAGATTTGCATGGATGACTCGTAAATCTGAAGAGACTATCACCCCATGGAATTTTGAGGAAGTTGTTGATAAGGGAGCCTCCGCTCAATCATTCATCGAGAGAATGACCAATTTCGACAAAAACTTGCCAAATGAGAAGGTGTTACCTAAACATTCTTTGTTATATGAATATTTCACTGTTTATAATGAATTAACAAAAGTCAAATACGTCACTGAAGGTATGAGAAAACCAGCATTCTTAAGTGGTGAACAAAAGAAAGCTATCGTTGACTTGTTATTCAAAACTAATCGTAAAGTCACTGTCAAACAACTTAAAGAGGACTATTTTAAGAAAATAGAGTGTTTCGATTCCGTTGAAATCAGTGGGGTTGAGGATAGATTTAATGCTAGTTTAGGAACTTATCACGATTTGTTGAAAATTATCAAAGACAAAGATTTTTTGGATAATGAAGAAAACGAAGATATCTTGGAGGACATTGTATTGACTTTGACTTTGTTTGAGGATAGAGAAATGATCGAAGAACGTTTAAAAACTTACGCTCACTTATTCGACGATAAAGTCATGAAACAATTAAAAAGAAGAAGATATACCGGTTGGGGAAGATTATCTAGAAAGTTGATTAATGGTATTAGAGATAAGCAAAGTGGTAAAACCATCTTAGATTTCCTTAAAAGTGATGGTTTTGCAAACAGAAACTTTATGCAGTTGATTCATGATGATAGTTTGACCTTTAAGGAAGATATTCAAAAAGCTCAGGTTTCTGGACAGGGTGATTCATTGCATGAACATATTGCCAACTTAGCAGGGTCTCCAGCTATCAAAAAGGGAATTTTACAAACTGTTAAAGTTGTTGATGAGTTGGTGAAAGTTATGGGACGTCATAAGCCTGAAAATATAGTTATAGAAATGGCTAGAGAAAATCAAACAACTCAGAAAGGACAAAAGAATTCTCGTGAAAGAATGAAAAGAATAGAAGAAGGAATTAAAGAGTTGGGTTCTCAAATCTTAAAAGAACACCCAGTCGAAAACACCCAGTTACAGAACGAGAAGCTTTACTTGTACTATTTGCAAAACGGGCGTGACATGTATGTAGATCAAGAGTTGGATATCAATAGACTTTCAGATTACGATGTCGATCACATAGTTCCTCAGTCATTTTTAAAAGATGATTCCATTGACAACAAAGTTTTAACAAGATCTGATAAGAATAGAGGTAAATCAGACAATGTGCCATCAGAAGAAGTGGTTAAGAAAATGAAGAACTATTGGAGACAATTGTTAAATGCAAAGTTGATTACTCAGAGAAAATTTGACAACTTAACTAAAGCTGAAAGAGGAGGATTATCCGAATTAGATAAAGCTGGATTCATAAAACGTCAGTTGGTTGAAACAAGACAAATTACTAAACATGTAGCCCAAATTTTAGATTCCCGAATGAATACTAAATATGATGAAAATGACAAATTAATCAGAGAGGTCAAAGTGATCACATTAAAGTCAAAGTTGGTGTCAGATTTCCGAAAGGATTTTCAATTCTACAAGGTGAGAGAGATCAACAATTATCATCATGCTCACGACGCATATTTGAATGCAGTGGTAGGAACTGCCTTAATAAAGAAGTATCCTAAGTTAGAATCCGAATTCGTGTATGGTGATTATAAAGTCTACGATGTCAGAAAGATGATAGCAAAATCAGAACAAGAAATTGGGAAAGCCACCGCAAAGTATTTCTTTTACTCCAACATCATGAATTTCTTTAAGACCGAAATCACCTTGGCTAACGGGGAAATCAGAAAACGTCCTTTGATTGAAACAAATGGAGAAACCGGTGAAATTGTGTGGGATAAGGGGAGAGATTTTGCTACTGTTAGAAAAGTTTTATCAATGCCTCAAGTTAATATTGTAAAGAAGACTGAAGTTCAAACTGGTGGTTTCTCTAAGGAATCCATTCTTCCAAAACGAAACTCAGACAAGTTGATCGCCCGAAAAAAAGATTGGGATCCTAAGAAATACGGAGGTTTTGATTCTCCTACTGTCGCATATTCTGTCTTGGTCGTGGCTAAAGTTGAAAAGGGTAAATCCAAAAAATTGAAATCTGTGAAAGAATTGTTAGGTATTACCATCATGGAGCGTTCATCCTTTGAGAAAAACCCAATTGACTTTCTCGAAGCGAAGGGCTATAAAGAAGTGAAGAAAGATTTGATTATTAAGTTGCCAAAGTATTCTCTCTTTGAACTTGAAAATGGGAGAAAGAGAATGTTGGCTTCAGCTGGAGAATTGCAAAAAGGAAACGAGTTAGCTTTGCCTTCAAAGTACGTTAATTTCTTATACTTAGCATCTCATTATGAAAAGTTGAAGGGGTCCCCTGAAGACAATGAGCAAAAGCAATTATTCGTAGAACAACATAAACACTATTTGGATGAGATAATTGAACAAATCTCCGAGTTTTCTAAAAGAGTTATTTTGGCTGATGCAAATTTGGATAAAGTTTTATCTGCTTATAATAAGCATCGTGACAAACCAATTCGAGAACAAGCTGAAAATATTATCCACTTGTTCACATTGACTAACTTGGGAGCTCCAGCCGCGTTTAAGTACTTTGACACCACCATTGACCGTAAGAGATATACTTCAACAAAAGAAGTGTTAGACGCCACATTGATTCATCAGTCCATAACTGGTTTATATGAAACTAGAATCGATTTGTCACAATTGGGGGGTGATAGCCGAGCGGATCCTAAGAAAAAACGAAAAGTCTGACTCGAGGTCGACGGTATCGATAAGCTTGATATCGAATTCCTGCAGCCCGGGGGATCCGAGTGAAATTCTGGAAATCTGGAAATCTGGTTTTGTATTCTTGTTATTCTTCTTTTTGTTATTACATATATAACTTGTTACTTTTTTAAAAAAATCTTTGTTTATTTTATAAATATATAAAACTAAATTTAAGAAAAAGAGAAAAATGTTTTATTTGAGAGATTGAAATTTTACTTGAATTTAGCTTAGCTTTTATAAAGTATTATTATGTAAAAAAACAAAACAAATATACATTAAAAAGTTAAGACTATAAAATAGCCACCCAAGGCATTTCTATATCTTGTTGTTGTTGTTTTCATCTTCTGTATCAGAGGAACTTATTTTATTATTTTCGTCACGGGTATTTTCTCTTGTTTGATGATTCATCCCATTCATTCCATCATAAAATTCTAGAAGGACCACCTTTGATTGTAAATAGTAATAATTACCACCCTTATTTAATTATTTATTTAACTTATTTATTTATTTATTATACATATATACAAATCTAATAAAGTGAAAATCTCCCCCTTCACATTTATAATTGGCCAGTTTTTTTCAAATAAGCATTCCAACCAGCATCTCTATACCTTTTACCTTCAATATCTGGATCTCTTCCTTTACCAAACAATCCTCTACCAACAATGATAATATCAGTTCCAGTGCTAACAACTTCATCAACAGTTCTATATTGTTGTCCTAATCCATCACCTTTATCATCTAATCCAACTCCAGGTGTCATAATAAGCCAATCAAATCCTTCTTCTTGTCCACCCATATCACGTTGGGCAATAAATCCAATAACAAATTCCTTATCGGATTTAGCAATTTCAACAGTTTTTTGAGAATATTCTCCATACGAATTCGAGCTCGTTTAAACGTCGACACTGGATGGCGGCGTTAGTATCGAATCGACAGCAGTATAGCGACCAGCATTCACATACGATTGACGCATGATATTACTTTCTGCGCACTTAACTTCGCATCTGGGCAGATGATGTCGAGGCGAAAAAAAATATAAATCACGCTAACATTTGATTAAAATAGAACAACTACAATATAAAAAAACTATACAAATGACAAGTTCTTGAAAACAAGAATCTTTTTATTGTCAGTACTGACTCGAGTTATTATGGACATGGCATAGACATATACAAAGCTTGTTCACCATCGGAAGCAGTACCATCGTATAAAGCAGTATCCAAACCACACAAAGTGAAACCCATTCTTCTATAAGCATGAATAGCTGGAGCATTAACATTGGTAACTTCCAACCACAAATGACCAGCACCTCTTTCTCTGGCGAATTCAGTAGCCAAACCCATCAAAGCTCTACCAACACCATGACCTCTATGTTCTGGAGCAACTTCAATATCTTCAACAGTCAATCTTCTGTTCCAACCAGAATAAGAAACAACAACGAAACCAGCCAAATCACCATCATCACCATAAGCAACGAAAGTTCTAGAATCTGGATCACCATCTTCACCAGCATCGGATTCATCATCGGATTCATCATCTGGGAAAACCTTAGTCAATGGTGGATCAACTGGAACTTCTCTCAAAGTGAAACCATCACCAGTAGCAGTAACTCTAAAAACAGTATCGGTAGTGAAAGAACCATCCAAAGCTTCAATAGCTTCAGCATCACCTGGAACAGAAGTTCTGTATCTATAAGCAGTATCATCCAAAGTAGTAGACATAATTGTAGGATCCGGTTGTTTATGTTCGGATGTGATGTGAGAACTGTATCCTAGCAAGATTTTAAAAGGAAGTATATGAAAGAAGAACCTCAGTGGCAAATCCTAACCTTTTATATTTCTCTACAGGGGCGCGGCGTGGGGACAATTCAACGCGTCTGTGAGGGGAGCGTTTCCCTGCTCGCAGGTCTGCAGCGAGGAGCCGTAATTTTTGCTTCGCGCCGTGCGGCCATCAAAATGTATGGATGCAAATGATTATACATGGGGATGTATGGGCTAAATGTACGGGCGACAGTCACATCATGCCCCTGAGCTGCGCACGTCAAGACTGTCAAGGAGGGTATTCTGGGCCTCCATGTCGCTGGCCGGGTGACCCGGCGGGGACGAGGCAAGCTTGATATGCTAATGATCCCACTGATGATAATTCAGCTAACATCAATAACCCTCTTGGCTCTTGGTTGGTGGTGGTTTCTTTAGCTCCCTGTTTTAATCCTTCAACTACTCCATTCCCAGTGACACCATGAGCATTAGTAATATCTGCCCAACTACTAATTTTATAAACTCCACCAATATATTGTTTCTTCACGGTATTACCAATATCAGCAAATTTTCTATCTTCAAAAATCATAAATTGATGTTTACGTGAAAGTTCTAATAATGGTTCAATAGTGGATTCATAGGAAAAATCATTGATTATATCAATATGAGTCTTGATTAAGCATACATAAGGACCCAATTTATCAATTAATTCAAGGAATTCCTTAGTGGTATCAACATCAATTGATGCACATAAATTGGTTTTCTTCAGTTCCATTAATCGAAATAATCGTTGTGCTACTGGTGAGGCATGAGTTTCTGCTCTCTCACTATAGGTCTTAGTGTTGACTGTCATATCTTTTGTTTGCTATTTCTATTTCTATTTCTTTCTTCAACTGTAAAAGGGGCGATATATAAACCAGTGTGTATGGGGTTGTTGCTCAGGAATTGATCAATGTTTAAAAGAATGGTTGAAATCATCATTGAAGAACAAAAAAAAAGATTTTTTCTTTCATTATGTTATCTGTATAATATGCAGGAAATAAGATTGCAAAAAAATAGCCGTTTTTTTGGATACTATCAAACAAGAGGAATGACTAATACAGAATTAGTGTTACGAATCAATGGCACTACAGCAACTTTCAACAAAATAATCTACGTTCAAAATATAATATATACTATTAAATAAAATCAAATAATAAAATAATTTTAAAGAAAGTAGTACCAGCTCTTTTTTTTGTTTCCGTTTATACCATCCAAATCAATTCCTATTAGTCTCCAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCTTTAAATGATAATTATAGTATTCACGGATAGATCTGTTAGAGATGTGGTGGATTGGTATTTCTTTCTGTGATTCACCGAAAGTATTATCAACTAAGAAGGGAAAGGCACCACATTTTCTAATGTCTCATATATTAGTTCAGTTCGATGTATTTCTTTTTATATATTAATTATAATTTTATTGCACCATAACGTTTACTTGTTTAATATGCTATTGATATCTATATGCGGCCAGACATAAAAAACAAAAAAAGCACCACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAACATTAGGTCGTATGATTGTTGCGCACCACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAACATTTCTTCTAAAGTTTCTGCCAAATTAAAAATAGTTTACGCAAGTCTCGAACTTCCACCACAATTTAATTGTAAGAACGCTGCGAAAACATATTCTCCAATCCTATTGGGCCGAAGTTTCAGCTATGCCAAAGGCATCAAATTTTTTTTTATAAAATTTTCCACTTTTCCATACCAGTGAAATTATCACGTGATGGAGTGAGTATCTATAATATTTGATTGTTTCTTTGATGAGGCAATACTCGAGTATAGTTATTGACCAATGACTTTTAGCTATGTGTAGTGCTTGTATATGCGTGTGTGATGAGAATAAGATGAATAGATTAGTAATGTATAAGTTGATTCTTTTTGGTGGGGGGTGTTACTTTTATTTTTTCTTTCCATCTCATTGACAAGATTGTACCGAGTCAAAATGTTTGGGGTCTTTTTATTGCTGCGATGGATTGATTTTTCACTCTAAGCCTAAAATTAAATGGCTAAACAAGTGGTATTCAAGCACAATTCAGTTTTCTGGATCTCTAATAATTGCAATTGCAATTGCTTCACATACTCTGTTTATAATTCAGTTTCTTTCTCTTGATGTTACTGATATATTAAGTTAATAAATACCAATAACTTTTTTTCAAAAATCTTCTATTTTCCTTTCAATTGATTATTTAAACACGAATCGTTAATAAGCTGTGATTGCTTTTTCTCTTTCTTTTTCTTTTCTTTGTTCAAGTTTGAAAAAAAAAAAAAAAGGTTTCTCTTGTTCGATGTAGAATTCAGTGGGTTATAGTTGTAATGATATATGGAATAAAAAGAAAGAAAGAGAAGAGAAAGAGAAGAAGAGAAGAATTAAGTAAACGTGTATTAGAATTTAAAAATTAAAATTCATGATAATCTTTAATAATAATTAACTAAGAATCACAAAATTGAAAATTTGAAAGAAAAAAGATCACTCCAAGGTCGTGTCAAGTGACTAGAAATTCTTAATTAAAATAGTTATAACAAAAAAAGAAATTTGTATATAACAATTAAGTAAAAGAAAAAAAAGCAATTCGCTTTGTATAATAATACCCAGAAAAATATATTCATATATTCATAAATCAAACATGAAATGAAGAATGCTGAATCACTTGATAGGATTTAGTTCCATTATGGTACTAAGAAGTAATATTACTTCTATGAAAAGGTTGAATCCGAGATCAAAATTCATCTTTTCATATACTCTGGAGAAAAAAAAAAAAGTAATTGTAGTAAGAATGACAAGTATCAGTTGAGAAAAAAAGTAACAAAGAGAAAGCTCGGAGGAGGCTCCCCAAAGGTTTTATCACCAATGGTGGTACCACTAACCCAAGAACAGAAAAAGCATTGATATTTTGAAAAATACAATACTCATCTTTTAACCCAAACCTTTTCTTGTCCTTTAAAACTTTTATCTCATGTCTGTCCAAAGTCATATTGTCTTACTACTTTCTCTCCTTCAACGTCCTTCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTTAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTCGAAATTAACCCTCACTAAAGGGAACAAAAGCTGGTACCAGAGCTATTAAGATCACCAGCCTCGTTTATAATAAACTTAGTCTAATATTTCTTTTGGACCAAAATAGAAACTGTTCTCAGGTGGCAAAAAACAGCTTAAAGTCTGTGTTAAAAAAAGATCTCGGCTATGCCAATCAAAAAGGAGTGACATTATTATGTAAAAGAAGAAATGTTGTAATTCCGCGAAACAACAATTTTAATGCTTACTGTGCCGCCACCAATATCCTATGGCCAAGGGGCCTTTAAACATGCCACGGAAATATACAGTCAAAGTAAACCTACTATGTCTTTAGAGCCTTCAGGATCAATTGTTTTTAAATGAATGACTGCAAAACTAGAAAAGTAGCCTCTTACATAAGCAAGTCATTTGTACACCGAGAGTTTAGTAACCAGTGAAGCAAGTATATCTGGCAAACCTGAAACTTATCTGTGAATTATAAAGCTCATAAGTAAAAAGTGTAGAAAAGAAGATAAGGCAGACCAAATACAAATTTGCAACAGTACAACGAATATTTCAGCGTCAAAACTAGAGAATAATAAAGAAAACGATCTTTTCAAAAAGAAAAAACCTTTTAGTTTTCCTTTGTTGTTGTTGTGGGTGTGTGCTATTTATATTATATAGTTTACTCATAATACCATAAAATATTCGGTTTGATTAGGTTATTTTAATAAGCTAATTTGTTTCTAATCGTGTAATTTATGCTGTGTATATTAAGTAGTGTGTGCACTGCCCAAAAATGTTTGTTGTTTATAGTCGGTTAAAGAGAAAAAAGAAAAAAAGATCCATACACACACGTTAATTAGTTGTTCAACGTAATACACTCATATTTTGTTCTTATTTGCTTTCGGTCGCTGTTCTCACCAAGATTTATTGCCAACGAAACAATTTTTTTTTATATATTTTCAGATTTTTCTTTTTTTCCTTTCCTTTCCTTTTCTAATTTTCACTCCTGGTTTTCTTTCTTTCTTAGAAACATTATCTCGATATTAATATTAAAAAAATATAATCATTCAAA
质粒元件
CDS 1..4104
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/note="在DNA中产生RNA引导的双链断裂"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
SV40 NLS 4117..4137
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/translation="PKKKRKV"
primer_bind 4143..4159
/note="常见的测序引物，多种相似变体之一"
terminator 5095..5292
/note="Ashbya棉蚜TEF终结者"
TEF promoter complement(5893..6236)
/note="棉蚜TEF启动子"
T7 promoter complement(7200..7218)
/note="噬菌体T7 RNA聚合酶启动子"
primer_bind complement(7225..7241)
/note="常见的测序引物，多种相似变体之一"
terminator 7742..7761
/note="酿酒酵母SUP4-tRNA基因的转录终止子"
misc_RNA complement(7765..7840)
/note="化脓性链球菌CRISPR/Cas9系统的RNA支架"
misc_RNA complement(7865..7940)
/note="化脓性链球菌CRISPR/Cas9系统的RNA支架"
primer_bind complement(8222..8241)
AmpR promoter 9771..9875
/gene="bla"
AmpR 9876..10736
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="对氨苄青霉素、羧苄青霉素和相关抗生素产生耐药性"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
ori 10907..11495
/direction=RIGHT
/note="高拷贝数ColE1/pMB1/pBR322/pUC复制起点"
CAP binding site 11783..11804
/bound_moiety="E. coli catabolite activator protein"
/note="CAP结合在cAMP存在下激活转录。"
lac promoter 11819..11849
/note="大肠杆菌lac操纵子的启动子"
lac operator 11857..11873
/bound_moiety="lac repressor encoded by lacI"
/note="lac阻遏物与lac操作员结合，抑制大肠杆菌中的转录。这种抑制可以通过添加乳糖或异丙基β-D-硫代吡喃半乳糖苷（IPTG）来缓解。"
primer_bind 11881..11897
/note="常见的测序引物，多种相似变体之一"
T3 promoter 11918..11936
/note="噬菌体T3 RNA聚合酶启动子"
