产品声明
For Research Use Only research use only . They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.Plasmid Sequence and Experimental Performance reference/template plasmid . MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.Sequence Verification of Certain Vector Products key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.After-Sales Period for Small-Pack Plasmid Products three months from the date of receipt . Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.Research-Related Risks and Limitation of Liability Citation in Chinese Publications 产品名称（产品货号）由淼灵质粒平台提供。 Citation in English Publications “Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”
质粒序列
一键复制序列（有部分质粒是非全长序列）
GACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCGAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTTGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTCCGAATTCGCCCTTCACCATGGCTCCTAAGAAGAAGCGGAAGGTTGGTATTCACGGGGTGCCTGCGGCTTCAAAGCTCGAGAAATTCACCAACTGTTATTCGTTGAGCAAAACACTGCGGTTTAAAGCGATTCCAGTCGGCAAGACTCAAGAGAATATAGACAATAAGCGGCTGTTGGTGGAAGATGAAAAGCGCGCGGAAGACTACAAAGGGGTGAAGAAGTTGTTGGACAGATACTACCTCTCTTTTATCAATGATGTCTTGCACTCAATCAAATTGAAGAATCTGAACAACTACATCTCCCTCTTCAGAAAGAAAACAAGGACAGAAAAGGAGAATAAGGAACTTGAAAATTTGGAGATCAATCTGAGGAAAGAGATCGCGAAAGCCTTTAAAGGCAACGAAGGATACAAAAGTCTGTTCAAGAAGGATATAATTGAGACAATTTTGCCAGAGTTCCTCGATGACAAGGACGAGATTGCGCTGGTCAATTCGTTCAACGGATTCACAACAGCATTCACAGGCTTCTTTGATAATCGGGAAAATATGTTCTCTGAGGAGGCAAAGTCCACTTCTATTGCGTTCAGGTGTATCAATGAGAATCTCACTAGGTACATTTCCAACATGGATATCTTTGAGAAGGTTGACGCAATTTTTGACAAGCACGAAGTTCAGGAGATTAAGGAGAAGATCCTCAATTCCGATTATGACGTTGAGGACTTCTTCGAAGGTGAGTTTTTTAATTTCGTGCTCACTCAAGAGGGTATCGACGTGTATAATGCGATCATCGGTGGGTTCGTGACTGAGTCCGGTGAAAAGATTAAGGGATTGAACGAGTATATCAACCTTTACAACCAAAAGACGAAACAGAAGCTGCCAAAGTTCAAGCCTCTTTACAAACAGGTTCTTTCAGACCGCGAGTCACTCTCGTTCTATGGGGAGGGCTACACTTCGGATGAGGAAGTCCTGGAGGTGTTCAGGAATACTCTCAATAAGAATTCGGAGATTTTCTCTTCTATAAAAAAACTGGAAAAGTTGTTTAAGAATTTTGACGAATACTCTAGCGCCGGCATATTTGTGAAAAACGGCCCGGCCATATCAACGATAAGTAAAGATATCTTCGGCGAATGGAACGTGATCAGAGACAAATGGAACGCGGAGTATGACGATATTCACCTGAAGAAGAAGGCTGTCGTAACGGAGAAGTACGAGGATGATCGCAGGAAAAGCTTCAAAAAGATCGGAAGTTTCAGCCTGGAACAGTTGCAGGAGTATGCTGACGCCGATCTTAGCGTCGTCGAGAAGTTGAAGGAGATAATCATCCAAAAGGTCGACGAGATATATAAAGTCTATGGATCAAGTGAAAAACTGTTCGACGCCGACTTCGTTTTGGAGAAGTCCCTGAAGAAGAACGACGCTGTTGTTGCCATTATGAAGGATCTGCTCGACAGCGTGAAGAGTTTCGAGAACTATATTAAGGCTTTTTTCGGGGAGGGGAAGGAGACTAACAGAGATGAGTCCTTCTACGGAGACTTCGTCCTCGCGTACGATATACTCCTTAAGGTAGACCACATCTACGACGCAATCAGAAATTACGTGACACAAAAGCCGTACAGCAAGGACAAGTTCAAACTCTACTTCCAGAACCCCCAGTTCATGGGCGGCTGGGACAAGGACAAGGAAACGGATTACAGGGCTACGATCCTGAGGTATGGTTCAAAATACTACTTGGCGATTATGGACAAGAAGTACGCCAAGTGTCTCCAGAAGATTGACAAAGACGATGTCAATGGCAATTATGAGAAGATCAACTACAAGCTGCTTCCGGGTCCGAACAAGATGCTCCCAAAGGTTTTCTTCAGCAAGAAATGGATGGCCTACTATAACCCAAGCGAGGACATCCAGAAGATTTATAAGAACGGTACGTTCAAGAAGGGCGACATGTTCAATCTTAACGACTGTCACAAGCTGATCGACTTCTTCAAAGACTCAATTAGCCGGTACCCAAAGTGGTCTAACGCCTATGACTTCAACTTTTCGGAAACCGAGAAGTACAAGGATATAGCCGGATTTTATAGAGAGGTGGAAGAGCAGGGCTACAAGGTGTCATTCGAGTCCGCCAGCAAGAAGGAAGTGGACAAGCTCGTGGAAGAGGGTAAGCTCTACATGTTCCAGATTTATAATAAAGACTTTAGCGATAAGAGCCACGGGACACCTAATCTCCACACAATGTATTTCAAGCTGCTCTTCGACGAGAATAACCACGGCCAAATCAGGTTGTCAGGAGGGGCTGAACTCTTCATGCGGCGCGCTAGCCTTAAGAAGGAGGAGCTTGTAGTCCACCCTGCGAATAGTCCAATTGCGAATAAGAACCCGGACAATCCTAAAAAGACTACAACATTGAGCTACGACGTGTACAAGGATAAGAGGTTTTCCGAGGATCAGTACGAGCTCCACATCCCGATTGCGATCAACAAGTGCCCAAAGAATATTTTCAAGATAAACACAGAGGTGCGTGTACTCCTGAAGCATGACGACAATCCTTACGTCATTGGGATTGATCGGGGCGAGAGGAACCTCCTCTATATTGTGGTGGTGGACGGGAAGGGGAACATAGTCGAACAGTACTCCCTTAACGAAATAATTAACAATTTCAACGGCATCCGTATCAAGACCGACTACCATTCGTTGCTGGACAAGAAGGAGAAGGAGAGATTTGAGGCGCGGCAAAATTGGACAAGTATCGAGAACATCAAGGAACTCAAAGCAGGTTATATCTCTCAAGTTGTGCATAAGATATGCGAGCTGGTTGAGAAGTATGACGCAGTGATCGCTCTTGAGGACCTCAACTCGGGCTTTAAGAATTCTAGAGTTAAAGTGGAGAAGCAGGTCTATCAAAAGTTCGAGAAGATGCTTATAGATAAGCTCAACTACATGGTCGATAAGAAATCGAACCCATGTGCCACCGGCGGCGCACTCAAAGGTTACCAAATAACAAACAAATTCGAGTCCTTCAAATCGATGAGTACTCAGAATGGGTTCATATTTTATATACCGGCGTGGCTTACGTCTAAGATCGACCCGTCAACTGGTTTTGTCAACCTGTTGAAGACGAAATACACGTCCATTGCCGATTCGAAAAAGTTCATATCTAGTTTTGATCGTATTATGTACGTCCCAGAGGAAGATCTTTTCGAGTTTGCTCTCGACTACAAAAACTTTTCGCGGACCGATGCGGATTACATTAAAAAATGGAAACTCTATTCGTACGGCAACAGAATCAGGATTTTTCGCAACCCTAAGAAGAATAACGTCTTTGATTGGGAGGAAGTTTGCTTGACTAGCGCGTACAAGGAGCTCTTTAATAAGTATGGCATTAACTACCAACAGGGTGATATCAGAGCACTGCTTTGCGAACAATCTGACAAGGCTTTCTACTCATCCTTCATGGCTTTGATGAGCCTGATGCTCCAGATGAGAAATTCAATTACAGGCAGAACCGACGTGGATTTCTTGATCTCCCCGGTTAAAAATTCTGATGGCATCTTTTACGATAGCAGGAACTATGAAGCGCAAGAGAATGCGATTCTGCCAAAAAATGCAGACGCCAACGGTGCCTATAACATCGCCAGGAAAGTCCTGTGGGCGATCGGCCAGTTCAAAAAGGCCGAAGACGAAAAATTGGACAAGGTCAAAATCGCTATCAGCAACAAAGAGTGGCTGGAGTATGCTCAGACATCCGTAAAGCATAAGCGTCCTGCTGCCACCAAAAAGGCCGGACAGGCTAAGAAAAAGAAGTGAGACGACTAGTGGCGGCCGCCGACGTCCGATCGTTCAAACATTTGGCAATAAAGTTTCTTAAGATTGAATCCTGTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATGTAATGCATGACGTTATTTATGAGATGGGTTTTTATGATTAGAGTCCCGCAATTATACATTTAATACGCGATAGAAAACAAAATATAGCGCGCAAACTAGGATAAATTATCGCGCGCGGTGTCATCTATGTTACTAGATCGGGAATTGATCCCCCCTCGACAGCTTCCGGAAAGGGCGAATTCGCAACTTTGTATACAAAAGTTGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCACTATGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTGGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATTATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAAC
质粒元件
terminator 562..589
/note="大肠杆菌rrnB基因的转录终止子T2"
terminator 681..767
/gene="Escherichia coli rrnB"
/note="大肠杆菌rrnB基因的转录终止子T1"
primer_bind 831..847
/note="常见的测序引物，多种相似变体之一"
attL1 863..962
/gene="mutant version of attL"
/bound_moiety="LR Clonase(TM)"
/note="Gateway（R）LR反应的重组位点"
SV40 NLS 987..1007
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/translation="PKKKRKV"
CDS 1032..4712
/codon_start=1
/product="CRISPR-associated protein from Lachnospiraceae
bacterium ND2006 (Zetsche et al., 2015)"
/translation="SKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAED
YKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKEI
AKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFSE
EAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEGE
FFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLS
DRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKNG
PAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQL
QEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKDL
LDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYSK
DKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNGN
YEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLI
DFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLVE
EGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKKE
ELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKIN
TEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTDY
HSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSGF
KNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQN
GFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDY
KNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQQ
GDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRNY
EAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTSV
KH"
nucleoplasmin NLS 4713..4760
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/translation="KRPAATKKAGQAKKKK"
terminator 4791..5043
/note="胭脂碱合酶终止子和poly（A）信号"
attR5 5088..5211
/gene="mutant version of attR"
/bound_moiety="LR Clonase(TM)"
/note="Gateway（R）LR反应的重组位点"
T7 promoter complement(5229..5247)
/note="噬菌体T7 RNA聚合酶启动子"
primer_bind complement(5252..5268)
/note="常见的测序引物，多种相似变体之一"
SmR 5699..6490
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/note="赋予对壮观霉素和链霉素的抗性"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ori join(6583..6939,1..232)
/direction=RIGHT
/note="高拷贝数ColE1/pMB1/pBR322/pUC复制起点"
