产品声明
For Research Use Only research use only . They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.Plasmid Sequence and Experimental Performance reference/template plasmid . MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.Sequence Verification of Certain Vector Products key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.After-Sales Period for Small-Pack Plasmid Products three months from the date of receipt . Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.Research-Related Risks and Limitation of Liability Citation in Chinese Publications 产品名称（产品货号）由淼灵质粒平台提供。 Citation in English Publications “Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”
质粒序列
一键复制序列（有部分质粒是非全长序列）
AACAAAATATTAACGCTTACAATTTCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGCCAAGCTGATCTATACATTGAATCAATATTGGCAATTAGCCATATTAGTCATTGGTTATATAGCATAAATCAATATTGGCTATTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCCAATATGACCGCCATGTTGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACACCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCCCGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCATGTGCTCACAGGAAGAGTTCCAGGCACAGAGGAGCCAGCTGGTGGCACTGCTGATCTCAGGGTCCTTGGAGGGCTTTGAGAGCATCTTAGACTGGCTGCTGTCTTGGGATGTGCTCTCCAGGGAGGACTATGAGGGCCTCAGCCTCCCTGGCCAACCTCTCTCCCATTCTGCCAGGCGTCTGCTGGACACAGTCTGGAACAAGGGTGTTTGGGGCTGTCAGAAGCTCCTCGAAGCTGTGCAGGAGGCACAGGCCAACAGTCATACCTTTGAACTGTATGGGTCCTGGGACACTCACTCCCTTCATCCAACCAGAGACCTGCAGAGTCACCGGCCAGCCATTGTGAGGAGACTCTACAACCATGTAGAAGCCATGCTGGAGCTGGCAAGGGAGGGGGGGTTTCTGAGCCAGTACGAGTGTGAGGAGATCAGGCTGCCAATCTTCACGTCGTCTCAGAGGGCAAGAAGGCTGCTTGATCTCGCTGCGGTGAAGGCCAATGGACTGGCTGCCTTCCTTCTACAGCACGTCAGGGAACTACCAGCTCCACTGCCTCTGCCTTACGAGGCTGCTGAGTGTCAGAAGTTCATATCTAAGCTGAGGACCATGGTGTTGACTCAGTCTCGCTTCCTCAGTACTTACGATGGGTCGGAGAATCTTTGCCTGGAGGATATATACACGGAGAACATCTTGGAGCTGCAGACTGAAGTGGGCACAGCCGGGGCCTTGCAGAAGAGCCCTGCCATCCTGGGCCTGGAGGACCTCTTTGATACCCATGGTCACCTGAACAGAGATGCCGACACCATACTGGTGGTGGGCGAAGCAGGCAGTGGCAAGAGCACTCTTCTGCAGCGTTTGCACCTGCTGTGGGCAACAGGGAGGAGCTTCCAGGAGTTTCTCTTCATTTTCCCATTCAGCTGCCGACAGTTGCAATGCGTGGCCAAACCGCTGTCCCTGAGGACGCTGCTCTTTGAGCACTGCTGCTGGCCTGATGTCGCTCAGGACGATGTCTTCCAGTTCCTTCTTGACCATCCTGACCGTGTCCTGTTAACCTTTGATGGCTTGGACGAGTTCAAGTTCCGGTTCACCGACCGGGAGCGCCACTGCTCTCCAATTGACCCCACGTCAGTCCAGACTCTGCTCTTCAACCTTCTCCAGGGGAACCTGCTGAAGAATGCCTGCAAGGTGCTGACCAGCCGTCCGGATGCTGTGTCAGCGCTCCTCAGGAAGTTCGTTCGCACAGAGTTGCAACTGAAGGGCTTCTCTGAAGAGGGCATCCAACTGTACCTGAGAAAGCACCACCGGGAACCTGGGGTGGCAGACCGCCTCATCCAGCTGATCCAAGCCACCTCAGCCCTGCATGGTTTGTGCCACCTCCCTGTCTTCTCTTGGATGGTGTCCAGATGCCACCGGGAACTTCTGCTGCAGAACAGGGGCTTCCCAACAACCAGCACGGACATGTACCTCCTGATCCTGCAGCATTTCCTGCTGCATGCCTCCCCTCCGGACTCCTCCCCACTCGGTCTGGGACCTGGACTCCTCCAAAGCCGGCTCTCCACCCTCCTGCACCTTGGCCACCTGGCTCTCCGGGGCCTGGCCATGAGCTGCTATGTGTTCTCAGCCCAGCAGCTCCAGGCAGCTCAGGTTGACTCTGATGATATTTCTCTTGGTTTCCTGGTACGTGCCCAAAGTAGTGTGCCCGGGAGCAAGGCGCCCCTGGAGTTCCTGCACATTACCTTCCAGTGCTTTTTTGCCGCTTTCTACTTGGCTGTCAGTGCTGACACATCGGTGGCCTCTCTCAAGCACCTTTTCAGCTGTGGCCGGCTGGGCAGCTCACTGCTGGGAAGGCTGCTGCCCAACCTGTGTATCCAGGGCTCCAGAGTCAAGAAGGGCAGCGAAGCAGCCCTGCTGCAGAAGGCTGAGCCACACAACCTGCAAATCACAGCAGCCTTCCTAGCAGGTCTGTTGTCCCAGCAGCATCGGGACCTGTTGGCTGCATGCCAGGTCTCCGAGAGGGTACTGCTCCAGCGTCAGGCACGTGCCCGCTCGTGTCTGGCCCACAGCCTCCGCGAGCACTTCCATTCCATCCCGCCTGCCGTGCCCGGTGAGACCAAGAGCATGCATGCTATGCCGGGCTTCATTTGGCTCATCCGTAGCCTGTACGAGATGCAGGAGGAGCAGTTGGCCCAGGAGGCTGTCCGTCGCTTGGACATCGGGCACCTGAAGTTGACATTTTGCAGAGTGGGCCCTGCAGAGTGTGCTGCACTGGCCTTTGTACTGCAACATCTCCAGCGGCCTGTGGCCCTACAGCTGGATTACAACTCTGTGGGAGATGTTGGAGTGGAACAGCTGCGACCGTGCCTTGGGGTCTGCACAGCTCTGTATTTGCGAGATAACAATATCTCAGACCGAGGTGCCCGCACGCTGGTTGAGTGTGCTCTTCGCTGTGAGCAGCTGCAGAAACTAGCTCTCTTCAACAACAAACTCACGGATGCGTGCGCCTGCTCCATGGCCAAGCTCCTTGCACACAAGCAGAACTTCTTGTCCCTGAGGGTGGGGAACAATCACATCACAGCCGCTGGAGCCGAGGTGCTGGCCCAGGGACTCAAGAGCAACACCTCCCTGAAGTTCCTGGGGTTCTGGGGCAACAGCGTGGGTGATAAGGGCACCCAAGCCCTGGCTGAAGTTGTAGCCGACCACCAGAACCTAAAGTGGCTCAGCCTGGTAGGAAACAACATTGGCAGCATGGGTGCCGAAGCCCTAGCACTGATGCTGGAGAAGAACAAGTCACTAGAGGAACTCTGCCTAGAGGAAAACCATATCTGTGACGAAGGGGTATATTCTCTCGCAGAAGGACTCAAGAGAAATTCAACTTTGAAATTCTTGAAACTGTCCAACAATGGCATCACCTACCGGGGTGCAGAAGCCCTCCTGCAGGCCCTCAGCAGGAACAGTGCCATTCTGGAGGTTTGGCTTCGAGGGAACACATTCTCTTTGGAGGAAATCCAAACACTGAGCTCCAGGGACGCCAGACTCTTGTTGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAGGATGACGACGATAAGGTTTAAACGGCCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATTATGGGGTGGAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGGCCTGCGGGGTCTATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATCTCCGCCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGGCATGCATGACCAGGCTCAGCTAATTTTTGTTTTTTTGGTAGAGACGGGGTTTCACCATATTGGCCAGGCTGGTCTCCAACTCCTAATCTCAGGTGATCTACCCACCTTGGCCTCCCAAATTGCTGGGATTACAGGCGTGAACCACTGCTCCCTTCCCTGTCCTTCTGATTTTAAAATAACTATACCAGCAGGAGGACGTCCAGACACAGCATAGGCTACCTGGCCATGCCCAACCGGTGGGACATTTGAGTTGCTTGCTTGGCACTGTCCTCTCATGCGTTGGGTCCACTCAGTAGATGCCTGTTGAATTGGGTACGCGGCCAGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAACCTGAGGCTATGGCAGGGCCTGCCGCCCCGACGTTGGCTGCGAGCCCTGGGCCTTCACCCGAACTTGGGGGGTGGGGTGGGGAAAAGGAAGAAACGCGGGCGTATTGGCCCCAATGGGGTCTCGGTGGGGTATCGACAGAGTGCCAGCCCTGGGACCGAACCCCGCGTTTATGAACAAACGACCCAACACCGTGCGTTTTATTCTGTCTTTTTATTGCCGTCATAGCGCGGGTTCCTTCCGGTATTGTCTCCTTCCGTGTTTCAGTTAGCCTCCCCCTAGGGTGGGCGAAGAACTCCAGCATGAGATCCCCGCGCTGGAGGATCATCCAGCCGGCGTCCCGGAAAACGATTCCGAAGCCCAACCTTTCATAGAAGGCGGCGGTGGAATCGAAATCTCGTGATGGCAGGTTGGGCGTCGCTTGGTCGGTCATTTCGAACCCCAGAGTCCCGCTCAGAAGAACTCGTCAAGAAGGCGATAGAAGGCGATGCGCTGCGAATCGGGAGCGGCGATACCGTAAAGCACGAGGAAGCGGTCAGCCCATTCGCCGCCAAGCTCTTCAGCAATATCACGGGTAGCCAACGCTATGTCCTGATAGCGATCCGCCACACCCAGCCGGCCACAGTCGATGAATCCAGAAAAGCGGCCATTTTCCACCATGATATTCGGCAAGCAGGCATCGCCATGGGTCACGACGAGATCCTCGCCGTCGGGCATGCTCGCCTTGAGCCTGGCGAACAGTTCGGCTGGCGCGAGCCCCTGATGCTCTTCGTCCAGATCATCCTGATCGACAAGACCGGCTTCCATCCGAGTACGTGCTCGCTCGATGCGATGTTTCGCTTGGTGGTCGAATGGGCAGGTAGCCGGATCAAGCGTATGCAGCCGCCGCATTGCATCAGCCATGATGGATACTTTCTCGGCAGGAGCAAGGTGAGATGACAGGAGATCCTGCCCCGGCACTTCGCCCAATAGCAGCCAGTCCCTTCCCGCTTCAGTGACAACGTCGAGCACAGCTGCGCAAGGAACGCCCGTCGTGGCCAGCCACGATAGCCGCGCTGCCTCGTCTTGCAGTTCATTCAGGGCACCGGACAGGTCGGTCTTGACAAAAAGAACCGGGCGCCCCTGCGCTGACAGCCGGAACACGGCGGCATCAGAGCAGCCGATTGTCTGTTGTGCCCAGTCATAGCCGAATAGCCTCTCCACCCAAGCGGCCGGAGAACCTGCGTGCAATCCATCTTGTTCAATCATGCGAAACGATCCTCATCCTGTCTCTTGATCGATCTTTGCAAAAGCCTAGGCCTCCAAAAAAGCCTCCTCACTACTTCTGGAATAGCTCAGAGGCCGAGGCGGCCTCGGCCTCTGCATAAATAAAAAAAATTAGTCAGCCATGGGGCGGAGAATGGGCGGAACTGGGCGGAGTTAGGGGCGGGATGGGCGGAGTTAGGGGCGGGACTATGGTTGCTGACTAATTGAGATGCATGCTTTGCATACTTCTGCCTGCTGGGGAGCCTGGGGACTTTCCACACCTGGTTGCTGACTAATTGAGATGCATGCTTTGCATACTTCTGCCTGCTGGGGAGCCTGGGGACTTTCCACACCCTAACTGACACACATTCCACAGCTGGTTCTTTCCGCCTCAGGACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTT
质粒元件
primer_bind 166..182
/note="常见的测序引物，多种相似变体之一"
CMV enhancer 343..722
/note="人类巨细胞病毒即刻早期增强子"
CMV promoter 723..926
/note="人巨细胞病毒（CMV）即刻早期启动子"
T7 promoter 952..970
/note="噬菌体T7 RNA聚合酶启动子"
Myc 4086..4115
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/translation="EQKLISEEDL"
CDS 4134..4157
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/translation="DYKDDDDK"
primer_bind complement(4177..4193)
/note="常见的测序引物，多种相似变体之一"
hGH poly(A) signal 4204..4826
/note="人生长激素多聚腺苷酸化信号"
ori complement(4975..5563)
/direction=LEFT
/note="高拷贝数ColE1/pMB1/pBR322/pUC复制起点"
HSV TK poly(A) signal 5892..5939
/note="单纯疱疹病毒胸苷激酶多聚腺苷酸化信号（Cole和Stacy，1985）"
NeoR/KanR complement(6171..6965)
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="赋予对新霉素、卡那霉素和G418（遗传霉素（R））的抗性"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
SV40 promoter complement(7000..7357)
/note="SV40增强子和早期启动子"
SV40 ori 7014..7149
/note="SV40复制起点"
AmpR promoter complement(7359..7463)
/gene="bla"
f1 ori join(7490..7920,1..25)
/direction=RIGHT
/note="f1噬菌体复制起点；箭头表示（+）链合成的方向"
