pUC18-mini-Tn7T-Plac-dCas9 (Plac)
产品声明
For Research Use Only research use only . They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.Plasmid Sequence and Experimental Performance reference/template plasmid . MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.Sequence Verification of Certain Vector Products key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.After-Sales Period for Small-Pack Plasmid Products three months from the date of receipt . Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.Research-Related Risks and Limitation of Liability Citation in Chinese Publications 产品名称（产品货号）由淼灵质粒平台提供。 Citation in English Publications “Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”
质粒序列
一键复制序列（有部分质粒是非全长序列）
AGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTAGAGGACCAGCCGCGTAACCTGGCAAAATCGGTTACGGTTGAGTAATAAATGGATGCCCTGCGTAAGCGGGTGTGGGCGGACAATAAAGTCTTAAACTGAACAAAATAGATCTAAACTATGACAATAAAGTCTTAAACTAGACAGAATAGTTGTAAACTGAAATCAGTCCAGTTATGCTGTGAAAAAGCATACTGGACTTTTGTTATGGCTAAAGCAAACTCTTCATTTTCTGAAGTGCAAATTGCCCGTCGTATTAAAGAGGGGCGTGGGGTTCGAGGTCGACGGTATCGATAAGCTAGCTTAATTAGCTGAGCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAAGCTAGACTGCGATGAGTGGCAGGGCGGGGCGTAATTTTTTTAAGGCAGTTATTGGTGCCCTTAAACGCCTGGGGTAATGACTCTCTAGCTTGAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGCTAGGAGCTTGCGGCCCGGACGATCGAATTGGGGATCTTGAAGTACCTATTCCGAAGTTCCTATTCTCTAGAAAGTATAGGAACTTCAGAGCGCTTTTGAAGCTGATGTGCTTAAAAACTTACTCAATGGAATTCTAGATCGAATTGGCCGCGGCGTTGTGACAATTTACCGAACAACTCCGCGGCCGGGAAGCCGATCTCGGCTTGAACGAATTGTTAGGTGGCGGTACTTGGGTCGATATCAAAGTGCATCACTTCTTCCCGTATGCCCAACTTTGTATAGAGAGCCACTGCGGGATCGTCACCGTAATCTGCTTGCACGTAGATCACATAAGCACCAAGCGCGTTGGCCTCATGCTTGAGGAGATTGATGAGCGCGGTGGCAATGCCCTGCCTCCGGTGCTCGCCGGAGACTGCGAGATCATAGATATAGATCTCACTACGCGGCTGCTCAAACTTGGGCAGAACGTAAGCCGCGAGAGCGCCAACAACCGCTTCTTGGTCGAAGGCAGCAAGCGCGATGAATGTCTTACTACGGAGCAAGTTCCCGAGGTAATCGGAGTCCGGCTGATGTTGGGAGTAGGTGGCTACGTCTCCGAACTCACGACCGAAAAGATCAAGAGCAGCCCGCATGGATTTGACTTGGTCAGGGCCGAGCCTACATGTGCGAATGATGCCCATACTTGAGCCACCTAACTTTGTTTTAGGGCGACTGCCCTGCTGCGTAACATCGTTGCTGCTGCGTAACATCGTTGCTGCTCCATAACATCAAACATCGACCCACGGCGTAACGCGCTTGCTGCTTGGATGCCCGAGGCATAGACTGTACAAAAAAACAGTCATAACAAGCCATGAAAACCGCCACTGCGCCGTTACCACCGCTGCGTTCGGTCAAGGTTCTGGACCAGTTGCGTGAGCGCATACGCTACTTGCATTACAGTTTACGAACCGAACAGGCTTATGTCAATTCGATCCATTGCTGTTGACAAAGGGAATCAGGGGATCTTGAAGTTCCTATTCCGAAGTTCCTATTCTCTAGAAAGTATAGGAACTTCAGAGCGCTTTTGAAGCTAATTCGATCATGCATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTAGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCGCGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCACCATTCGATGGTGTCAACGTAAATGCCGCTTCGCCTTCGCGCGCGAATTGCAAGCTGATCCGGGCTTATCGACTGCACGGTGCACCAATGCTTCTGGCGTCAGGCAGCCATCGGAAGCTGTGGTATGGCTGTGCAGGTCGTAAATCACTGCATAATTCGTGTCGCTCAAGGCGCACTCCCGTTCTGGATAATGTTTTTTGCGCCGACATCATAACGGTTCTGGCAAATATTCTGAAATGAGCTGTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGAATTCGAGCTCCTGACTCAGGAGGTATCGATATGTACCCATACGATGTTCCAGATTACGCTATGACCAACGGCAAGATCCTGGGCCTGGCCATCGGCATCGCCTCGGTGGGCGTGGGCATCATCGAGGCCAAGACCGGCAAGGTGGTGCACGCCAACTCGCGCCTGTTCTCGGCCGCCAACGCCGAGAACAACGCCGAGCGCCGCGGCTTCCGCGGCTCGCGCCGCCTGAACCGCCGCAAGAAGCACCGCGTGAAGCGCGTGCGCGACCTGTTCGAGAAGTACGGCATCGTGACCGACTTCCGCAACCTGAACCTGAACCCGTACGAGCTGCGCGTGAAGGGCCTGACCGAGCAGCTGAAGAACGAGGAGCTGTTCGCCGCCCTGCGCACCATCTCGAAGCGCCGCGGCATCTCGTACCTGGACGACGCCGAGGACGACTCGACCGGCTCGACCGACTACGCCAAGTCGATCGACGAGAACCGCCGCCTGCTGAAGAACAAGACCCCGGGCCAGATCCAGCTGGAGCGCCTGGAGAAGTACGGCCAGCTGCGCGGCAACTTCACCGTGTACGACGAGAACGGCGAGGCCCACCGCCTGATCAACGTGTTCTCGACCTCGGACTACGAGAAGGAGGCCCGCAAGATCCTGGAGACCCAGGCCGACTACAACAAGAAGATCACCGCCGAGTTCATCGACGACTACGTGGAGATCCTGACCCAGAAGCGCAAGTACTACCACGGCCCGGGCAACGAGAAGTCGCGCACCGACTACGGCCGCTTCCGCACCGACGGCACCACCCTGGAGAACATCTTCGGCATCCTGATCGGCAAGTGCAACTTCTACCCGGACGAGTACCGCGCCTCGAAGGCCTCGTACACCGCCCAGGAGTACAACTTCCTGAACGACCTGAACAACCTGAAGGTGTCGACCGAGACCGGCAAGCTGTCGACCGAGCAGAAGGAGTCGCTGGTGGAGTTCGCCAAGAACACCGCCACCCTGGGCCCGGCCAAGCTGCTGAAGGAGATCGCCAAGATCCTGGACTGCAAGGTGGACGAGATCAAGGGCTACCGCGAGGACGACAAGGGCAAGCCGGACCTGCACACCTTCGAGCCGTACCGCAAGCTGAAGTTCAACCTGGAGTCGATCAACATCGACGACCTGTCGCGCGAGGTGATCGACAAGCTGGCCGACATCCTGACCCTGAACACCGAGCGCGAGGGCATCGAGGACGCCATCAAGCGCAACCTGCCGAACCAGTTCACCGAGGAGCAGATCTCGGAGATCATCAAGGTGCGCAAGTCGCAGTCGACCGCCTTCAACAAGGGCTGGCACTCGTTCTCGGCCAAGCTGATGAACGAGCTGATCCCGGAGCTGTACGCCACCTCGGACGAGCAGATGACCATCCTGACCCGCCTGGAGAAGTTCAAGGTGAACAAGAAGTCGTCGAAGAACACCAAGACCATCGACGAGAAGGAGGTGACCGACGAGATCTACAACCCGGTGGTGGCCAAGTCGGTGCGCCAGACCATCAAGATCATCAACGCCGCCGTGAAGAAGTACGGCGACTTCGACAAGATCGTGATCGAGATGCCGCGCGACAAGAACGCCGACGACGAGAAGAAGTTCATCGACAAGCGCAACAAGGAGAACAAGAAGGAGAAGGACGACGCCCTGAAGCGCGCCGCCTACCTGTACAACTCGTCGGACAAGCTGCCGGACGAGGTGTTCCACGGCAACAAGCAGCTGGAGACCAAGATCCGCCTGTGGTACCAGCAGGGCGAGCGCTGCCTGTACTCGGGCAAGCCGATCTCGATCCAGGAGCTGGTGCACAACTCGAACAACTTCGAGATCGACGCCATCCTGCCGCTGTCGCTGTCGTTCGACGACTCGCTGGCCAACAAGGTGCTGGTGTACGCCTGGACCAACCAGGAGAAGGGCCAAAAGACCCCGTACCAGGTGATCGACTCGATGGACGCCGCCTGGTCGTTCCGCGAGATGAAGGACTACGTGCTGAAGCAGAAGGGCCTGGGCAAGAAGAAGCGCGACTACCTGCTGACCACCGAGAACATCGACAAGATCGAGGTGAAGAAGAAGTTCATCGAGCGCAACCTGGTGGACACCCGCTACGCCTCGCGCGTGGTGCTGAACTCGCTGCAGTCGGCCCTGCGCGAGCTGGGCAAGGACACCAAGGTGTCGGTGGTGCGCGGCCAGTTCACCTCGCAGCTGCGCCGCAAGTGGAAGATCGACAAGTCGCGCGAGACCTACCACCACCACGCCGTGGACGCCCTGATCATCGCCGCCTCGTCGCAGCTGAAGCTGTGGGAGAAGCAGGACAACCCGATGTTCGTGGACTACGGCAAGAACCAGGTGGTGGACAAGCAGACCGGCGAGATCCTGTCGGTGTCGGACGACGAGTACAAGGAGCTGGTGTTCCAGCCGCCGTACCAGGGCTTCGTGAACACCATCTCGTCGAAGGGCTTCGAGGACGAGATCCTGTTCTCGTACCAGGTGGACTCGAAGTACAACCGCAAGGTGTCGGACGCCACCATCTACTCGACCCGCAAGGCCAAGATCGGCAAGGACAAGAAGGAGGAGACCTACGTGCTGGGCAAGATCAAGGACATCTACTCGCAGAACGGCTTCGACACCTTCATCAAGAAGTACAACAAGGACAAGACCCAGTTCCTGATGTACCAGAAGGACTCGCTGACCTGGGAGAACGTGATCGAGGTGATCCTGCGCGACTACCCGACCACCAAGAAGTCGGAGGACGGCAAGAACGACGTGAAGTGCAACCCGTTCGAGGAGTACCGCCGCGAGAACGGCCTGATCTGCAAGTACTCGAAGAAGGGCAAGGGCACCCCGATCAAGTCGCTGAAGTACTACGACAAGAAGCTGGGCAACTGCATCGACATCACCCCGGAGGAGTCGCGCAACAAGGTGATCCTGCAGTCGATCAACCCGTGGCGCGCCGACGTGTACTTCAACCCGGAGACCCTGAAGTACGAGCTGATGGGCCTGAAGTACTCGGACCTGTCGTTCGAGAAGGGCACCGGCAACTACCACATCTCGCAGGAGAAGTACGACGCCATCAAGGAGAAGGAGGGCATCGGCAAGAAGTCGGAGTTCAAGTTCACCCTGTACCGCAACGACCTGATCCTGATCAAGGACATCGCCTCGGGCGAGCAGGAGATCTACCGCTTCCTGTCGCGCACCATGCCGAACGTGAACCACTACGTGGAGCTGAAGCCGTACGACAAGGAGAAGTTCGACAACGTGCAGGAGCTGGTGGAGGCCCTGGGCGAGGCCGACAAGGTGGGCCGCTGCATCAAGGGCCTGAACAAGCCGAACATCTCGATCTACAAGGTGCGCACCGACGTGCTGGGCAACAAGTACTTCGTGAAGAAGAAGGGCGACAAGCCGAAGCTGGACTTCAAGAACAACAAGAAGTAAATACTCTGGCCATTTTTCTTTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAACGCGTGCGGCCGCTGATTAGCTAAGCAGAAGGCCATCCTGACGGATGCCTTTTTCGCTTGCTAGCTGCAGCCCGGGGGATCCCCCGGGCTGCAGGAATTCCTCGAGAAGCTTGGGCCCGGTACCTCGCGAAGGCCTTGCAGGCCAACCAGATAAGTGAAATCTAGTTCCAAACTATTTTGTCATTTTTAATTTTCGTATTAGCTTACGACGCTACACCCAGTTCCCATCTATTTTGTCACTCTTCCCTAAATAATCCTTAAAAACTCCATTTCCACCCCTCCCAGTTCCCAACTATTTTGTCCGCCCACAGCGGGGCATTTTTCTTCCTGTTATGTTTGGGCGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACCCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGG
质粒元件
primer_bind 534..550
/note="常见的测序引物，多种相似变体之一"
terminator 578..672
/note="λ噬菌体的转录终止子"
terminator 775..861
/gene="Escherichia coli rrnB"
/note="大肠杆菌rrnB基因的转录终止子T1"
FRT 907..954
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP介导的重组发生在8-bp核心序列TCTAGAAA中（Turan和Bode，2011）。"
GmR complement(1084..1617)
/codon_start=1
/gene="aacC1"
/product="gentamycin acetyltransferase"
/note="赋予对庆大霉素的抗性"
/translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
EEVMHFDIDPSTAT"
Pc promoter complement(1806..1834)
/gene="intI1 (promoter lies within the coding sequence)"
/note="1类整合子启动子"
FRT 1875..1922
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP介导的重组发生在8-bp核心序列TCTAGAAA中（Turan和Bode，2011）。"
lacI complement(1965..3047)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/note="lac阻遏物与lac操作员结合，抑制大肠杆菌中的转录。这种抑制可以通过添加乳糖或异丙基β-D-硫代吡喃半乳糖苷（IPTG）来缓解。"
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
lacIq promoter complement(3048..3125)
/gene="lacI (mutant)"
/note="在lacIq等位基因中，启动子中的单个碱基变化将lacI基因的表达提高了约10倍。"
lac promoter 3355..3385
/note="大肠杆菌lac操纵子的启动子"
lac operator 3393..3409
/bound_moiety="lac repressor encoded by lacI"
/note="lac阻遏物与lac操作员结合，抑制大肠杆菌中的转录。这种抑制可以通过添加乳糖或异丙基β-D-硫代吡喃半乳糖苷（IPTG）来缓解。"
HA 3461..3487
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/translation="YPYDVPDYA"
mobile_element 7099..7264
/mobile_element_type="transposon:Tn7"
/note="mini-Tn7元件（Tn7转座子的左端）"
ori complement(7534..8122)
/direction=LEFT
/note="高拷贝数ColE1/pMB1/pBR322/pUC复制起点"
AmpR complement(8293..9153)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="对氨苄青霉素、羧苄青霉素和相关抗生素产生耐药性"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
AmpR promoter complement(9154..9258)
/gene="bla"
