产品声明
For Research Use Only research use only . They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.Plasmid Sequence and Experimental Performance reference/template plasmid . MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.Sequence Verification of Certain Vector Products key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.After-Sales Period for Small-Pack Plasmid Products three months from the date of receipt . Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.Research-Related Risks and Limitation of Liability Citation in Chinese Publications 产品名称（产品货号）由淼灵质粒平台提供。 Citation in English Publications “Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”
质粒序列
一键复制序列（有部分质粒是非全长序列）
ATGAATGCCACAAACACCGATGTTTTCGCCCAGGTAGGCGGCCTGGAGGCCCGAGGCGCGAAGATGAAGAAGCGGGGCACCCGCTTCCTCATCGCGGCGCTGGCAGTCCTTGCCATTGCCGGGATCGGGGCAGTAACGGGATGGGCGATCAGCCCGAGCGCGACGCCCGGAAGCATTGACGTGCCGCAGGTGCTGGCATCGACATTCAGCGACCAGGTGCCGGGCAGTGAGGGCGGCGGCCTGGGTGGCGGCCTGCCCTTCACTTCGGCCGTCGGGGCATTCACGGACTTCATGGCGGGGCCGGCAATTTTTACCTTGGGCATTCTTGGCATAGTGGTCGCGGGTGCCGTGCTCGTGTTCGGGGGTGAGTTCTGCGGGTTCGTGCGATCCGTCTGCATGATGGTGATAGCCGTCAGCATGATTTTCGTGTCGTCGAACTTGGTGAAGGGCATTCTCGGCGGCGATCACGACGCCGGCCCTGCGGAGCCTTCGCCGCGTGCGCGATTCATGGCGGCCGTGGAGGCCAAGGATTTCGCGCGAGTGCAAGAGCTGATCGAGGCGCGTGGAGCCAAGTCGGCGGCTGATTATGTCCTTGCGCAGCTCGCCGTGGCCGAAGGTCTGGACCGCAAGCCTGGTGCGCGCGTCGTGGTCGGGAAAGCGGCGGGCAGCATGGCAATGCCGCCTGCGGCGCTGGGTTTTACGCCAAGGGGAGAAGCGGCATACGCCATCGAGCGGTCAGCCTATGGTGAGCCGAGGTCCAGCATTGCGAAGCAGTACCAGCAGGAATGGAACCGGAAGGCGGCGACCTGGTGGGCGATGGCCGGTGTGGCCGGCATCATCGGCGCGATCCTGGCGGCGGCGGCAACCGGCTTTGTTGGGCTGGCAGTGTCGATCCGCAACCGAGTGAAGCGCGTGCGCGACCTGTTGGTGATGGAGCCGGGTGCAGAGCCATAAGGGATCGATCCCCCTCAAGTCAAAAGCCTCCGGTCGGAGGCTTTTGACTTTCTGCTCGAGGAGCTAATTCCATTGAGTAAGTTTTTAAGCACATCAGCTTCAAAAGCGCTCTGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGGAATAGGTACTTCAAGATCCCCAATTCGAGCTCGGTACCCGGGAAGATCTGGCTAGCGGGGCCCTGATATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTATTAATTAATCCTCAATCGCTCTTCGTTCGTCTGGAAGGCAGTACACCTTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGCGCTGCTTCCCTGCTGTTTTGTGGAATATCTACCGACTGGAAACAGGCAAATGCAGGAAATTACTGAACTGAGGGGACAGGCGAGAGACGATGCCAAAGAGCTACACCGACGAGCTGGCCGAGTGGGTTGAATCCCGCGCGGCCAAGAAGCGCCGGCGTGATGAGGCTGCGGTTGCGTTCCTCCGCGGCAGATGTCTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAATGTCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTTGGTCACTGATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGATACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAACATGCCCGGTTACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGGATGCGGCGGGACCAGAGAAAAATCACTCAGGGTCAATGCCAGCGCTTCGTTAATACAGATGTAGGTGTTCCACAGGGTAGCCAGCAGCATCCTGCGATGCAGATCCGGAACATAATGGTGCAGGGCGCTGACTTCCGCGTTTCCAGACTTTACGAAACACGGAAACCGAAGACCATTCATGTTGTTGCTCAGGTCGCAGACGTTTTGCAGCAGCAGTCGCTTCACGTTCGCTCGCGTATCGGTGATTCATTCTGCTAACCAGTAAGGCAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAGGAGCACGATCATGCGCACCCGTGGGGCCGCCATGCCGGCGATAATGGCCTGCTTCTCGCCGAAACGTTTGGTGGCGGGACCAGTGACGAAGGCTTGAGCGAGGGCGTGCAAGATTCCGAATACCGCAAGCGACAGGCCGATCATCGTCGCGCTCCAGCGAAAGCGGTCCTCGCCGAAAATGACCCAGAGCGCTGCCGGCACCTGTCCTACGAGTTGCATGATAAAGAAGACAGTCATAAGTGCGGCGACGATAGTCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCTCAAGGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGGAATTCTCAGATTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGAAAGCTACTATTATTTGTTAACTGTTAATTGTCCTTGTTCAAGGATGCTGTCTTTGACAACAGATGTTTTCTTGCCTTTGATGTTCAGCAGGAAGCTCGGCGCAAACGTTGATTGTTTGTCTGCGTAGAATCCTCTGTTTGTCATATAGCTTGTAATCACGACATTGTTTCCTTTCGCTTGAGGTACAGCGAAGTGTGAGTAAGTAAAGGTTACATCGTTAGGATCAAGATCCATTTTTAACACAAGGCCAGTTTTGTTCAGCGGCTTGTATGGGCCAGTTAAAGAATTAGAAACATAACCAAGCATGTAAATATCGTTAGACGTAATGCCGTCAATCGTCATTTTTGATCCGCGGGAGTCAGTGAACAGGTACCATTTGCCGTTCATTTTAAAGACGTTCGCGCGTTCAATTTCATCTGTTACTGTGTTAGATGCAATCAGCGGTTTCATCACTTTTTTCAGTGTGTAATCATCGTTTAGCTCAATCATACCGAGAGCGCCGTTTGCTAACTCAGCCGGGCGTTTTTTATCGCTTTGCAGAAGTTTTTGACTTTCTTGACGGAAGAATGATGTGCTTTTGCCATAGTATGCTTTGTTAAATAAAGATTCTTCGCCTTGGTAGCCATCTTCAGTTCCAGTGTTTGCTTCAAATACTAAGTATTTGTGGCCTTTATCTTCTACGTAGTGAGGATCTCTCAGCGTATGGTTGTCGCCTGAGCTGTAGTTGCCTTCATCGATGAACTGCTGTACATTTTGATACGTTTTTCCGTCACCGTCAAAGATTGATTTATAATCCTCTACACCGTTGATGTTCAAAGAGCTGTCTGATGCTGATACGTTAACTTGTGCAGTTGTCAGTGTTTGTTTGCCGTAATGTTTACCGGAGAAATCAGTGTAGAATAAACGGATTTTTCCGTCAGATGTAAATGTGGCTGAACCTGACCATTCTTGTGTTTGGTCTTTTAGGATAGAATCATTTGCATCGAATTTGTCGCTGTCTTTAAAGACGCGGCCAGCGTTTTTCCAGCTGTCAATAGAAGTTTCGCCGACTTTTTGATAGAACATGTAAATCGATGTGTCATCCGCATTTTTAGGATCTCCGGCTAATGCAAAGACGATGTGGTAGCCGTGATAGTTTGCGACAGTGCCGTCAGCGTTTTGTAATGGCCAGCTGTCCCAAACGTCCAGGCCTTTTGCAGAAGAGATATTTTTAATTGTGGACGAATCAAATTCAGAAACTTGATATTTTTCATTTTTTTGCTGTTCAGGGATTTGCAGCATATCATGGCGTGTAATATGGGAAATGCCGTATGTTTCCTTATATGGCTTTTGGTTCGTTTCTTCCGCAAACGCTTGAGTTGCGCCGTCGCTCTGCGCGTGCGCCGACATGCAAGCGGCTGCGGTGAGCGCGGCGGCGCTCAGGGCCAAACGTTTGTGTCTACGGGAAACGGCTTGTTTGGAGTACGTCATCGTTTCCTCCTAACGCTTAATTGTTATCCGCTCACAATTCCACACATTAAACTAGATTCGATGATTAATTGTCAACAGCTCACTAGTTTAATTAATCAGAAGAACTCGTCAAGAAGGCGATAGAAGGCGATGCGCTGCGAATCGGGAGCGGCGATACCGTAAAGCACGAGGAAGCGGTCAGCCCATTCGCCGCCAAGCTCTTCAGCAATATCACGGGTAGCCAACGCTATGTCCTGATAGCGGTCCGCCACACCCAGCCGGCCACAGTCGATGAATCCAGAAAAGCGGCCATTTTCCACCATGATATTCGGCAAGCAGGCATCGCCATGGGTCACGACGAGATCCTCGCCGTCGGGCATCCGCGCCTTGAGCCTGGCGAACAGTTCGGCTGGCGCGAGCCCCTGATGCTCTTCGTCCAGATCATCCTGATCGACAAGACCGGCTTCCATCCGAGTACGTGCTCGCTCGATGCGATGTTTCGCTTGGTGGTCGAATGGGCAGGTAGCCGGATCAAGCGTATGCAGCCGCCGCATTGCATCAGCCATGATGGATACTTTCTCGGCAGGAGCAAGGTGAGATGACAGGAGATCCTGCCCCGGCACTTCGCCCAATAGCAGCCAGTCCCTTCCCGCTTCAGTGACAACGTCGAGCACAGCTGCGCAAGGAACGCCCGTCGTGGCCAGCCACGATAGCCGCGCTGCCTCGTCTTGGAGTTCATTCAGGGCACCGGACAGGTCGGTCTTGACAAAAAGAACCGGGCGCCCCTGCGCTGACAGCCGGAACACGGCGGCATCAGAGCAGCCGATTGTCTGTTGTGCCCAGTCATAGCCGAATAGCCTCTCCACCCAAGCGGCCGGAGAACCTGCGTGCAATCCATCTTGTTCAATCATACTCTTCCTTTTTCAAATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCGCATGCATCTAGAGGGATCCGCTGCAGGGCGGCCGCTCAGGTCAGCACGGTCATGAATCGTTCGTTGAGAATGCGGTCGTGGTAAAAGATCGCCTTGCCCAGCTGGTCGGTGGTCCAGGTCACCGGTTTGTGGTCCGGGTAGTTGTAATCTCCCCCGCAGAACACTTCGTTGCGGTTACCGGACGGGTCGAAGAAGTAGATGGTCTTGCCGTGAGTGAGGCCGTGGCGGGTTGGGCCGATATCGATAGATGTGTCGGTCATGGAGATCAGGTCGGCGGCGCGAAGCAAGTCTTCCCAGGTTTCGAGGTGGAAGGACACATGATGGAGGCGGCCTTTTTCCGGATGGTGAATGAAGGCCACGTCGTGGGCCTTGGTCGACAGACTGAGAAACTGGGCGACGCGCGTGCCATTTTCGTCCAGCACCTGTTCGGCCAGATAGAAACCGAGCACCTTGGTGAACAGGTCATAGGTCGCCGGCAATTCGTCGCCATACATGAGGGCGTGGTCGAAACGCACAGCCGCCATACCTTTCAGATCGCGCGGCCATGCCTCGGGATTGACGTCATTCAAACCCCACTTTCCAGTATATTCCTTGTCTGCATACAACTCGAAGTGATGCCCGGAGGGGGCCTGGAAGCGCACGCGCCGGCCACAACTGTTCAGTTCACCTGCGGGTAGCTGCTCAACGGCACAGCCATATGCCATCAGATCCCGCTCCAGTTGCCGGAGAGCATCCTCATCCACAACCTTGAAACCCATAAAATCCATGCCCGGCTCGTCAGCCTCGCGTAGCACCAGGGAAAACTTATCCACTTCGGTCCAAGCCTTCAGATAGACACGGCCCTGGTCGTCACGGTCCATCTCGATCAGGCCCAGCAACTCGACGTAGTGTTCCAGGGCCTTGCTCATGTCCAGTACACGCAGCTGCACATGGCCCGGTCGCATTACACCTTTGTTCATAGTATTCTGTCTCCTTCTGTGATATTCCACACATTATACGAGCCGATGATTAATGGTCAACAGCTCAAGCTTCCCGGGTACCGAGCTCGAATTAGCTTCAAAAGCGCTCTGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGGAATAGGAACTTCAAGATCCCCTGATTCCCTTTGTCAACAGCAATGGATATCAGCCGTTGACTTAGTTGGTATTTCCGGAATATCATGCTGGGTTCCGAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGGAAGAACAAGCGTGAACATGGCGCGATTGAAGGGGGAGGTTTTGCCCGCCCTCTTCGCGTCGCCGGCGACGATTGGCGAGTACGGGGCCGGCATCGACGGGGCGGATTCCCTCAACGAGCTGTCGAATCTGATGGAGCACGGCGCAGTTGCCGCGCTGGCCGACAAAATCAGCCAGATCGTGGCGAAGCTGGTCGACGCGGACCCCCGCAAGATCGCGGAAAAGCCTACCTGGTTCGAGAAGATGCTTGGCCGTGAGGTTGAACGCCAGGTGAGGTATCAGGTCGCCCGCAAGACGCTCGACCAGTTGCTGGACGAAGCCGAGGGCGTAGCGCAGCGCGTGCGGGACACGTTGCGCGCCTTGGATGACATGCTCAATACGCATGAGGCCGAGGTAGACCGGCTCAGAGCCTACATTCAAGCCGGGCGCGAGTTCCTGGACGAGAACCCCGAGGCCGGCGCGGCCAAGGCCGGCGTGATCGAGTTCGACAAGCCGCGCGAACGCTTCGCGCGCAAGCTCGCCAACCTGGCAACCCTCATGGCGTCCCATGAAATGAGCGTCACTCAGATGAAGCTCACGCGGGCGCAGGCCGTGGACATGCTGGACCGCTTCTCTGAAACGGCATCCGTCCTGGTGCCCGTCTGGCGTCAGCACACCCTCGCGCTCATCACCACCAAGAACATGAATCCGGCAATGGTCGCCGAGGCGGCCAAAGCTCACCAGGCGCTCATGCGGAGCCTTTCGCAGAGCCTGGAAGGCATCAACCAATAACACGGCGGGAGAACCCTATGAACGCACTGAAAACGACGCACGACGCCAAGGCCCCTATCGTCGCCTTTGACATGACCCCGGCAACCCTGCGCGAGCTGGGCTTGCAGGAAAGCGACGTGCCGGAAGTCCATGCGGTCGCGCAGCGGATCGAGGTCGGCAGTCCGCAGACCGTTGCCGAGTTCGGCCGCGACGTGGCCGAGCACACGTCCCGCTACGCCGATAGCCTGCTGGACCAGGTGCGCAACAGCGACCTGGACGAAGCAGGCGAGAAACTGACCCAGGTTGTCGCCAAGGCCCGTTCCCTGAACGTCGGCCCTTTGTCCGACAACCGTTCCCGCCTGCCCCTGATTGGCCCGCTGATCGACCGCTCCCGCGTCCGTTCGACGGGCTTCATGGCGCGCTTCGACACGACCCGCGAGCAGATCGAACACCTGGTCAGCGAAGTGCAGACCACCCAGCAAGGCATCGCGCAGCGCAATGCCTCGCTCGACGAAATGTTCGCAGCCGTGCGCGAGGAACACCGCCTTCTTGGCGTCCACATCGCGGCCGGCAAGGTCCGCCTTGCCGAGCTGCGCGAGCAGGCCGAGGGTCTGCGCGGCAATGTCGGGAACGACCCTGGCCGCGTGCAGGAGCTGGCCGACCTCGATGCGATGGTTGCCAACCTGGACAAGCGCATCGGCGACCTGATCGCCTTGCAACATTCGGCCATGCAGAGCCTGCCGACCATCCGCATGATCCAGGCCAACAACCAGATGCTGGTCGATAAATTCCACACCATCCGCGAAATCACCGTGCCGGCGTGGAAGCGGCAATTCATGCTGGCCTTGAGCCTCAACGAGCAGAAGAACGCCGTCGAACTGGCCACGGCCATCGACGACACCACCAACGACCTGATGAAGCGCAATGCGGCCCTGCTGCATCGCACGTCCGTCGAGACGGCGAAGGAGAACCAACGCCTGGTGATCGACGTGGACACGCTCAAGCAGGTTCAGACGACGCTCATCAAGACCGTCGAGGACGTTATTCGCATCCAGCAGGAAGGCGTGCAGAAGCGCAAGGATGCCGAGAAGCAGATCGCCGCAATGCGTGGCGATCTTCAAGCCAAGCTGACCCGCCAGCCCGTGCGCGAGCTGGCCCAACAGGAGTCCGT
质粒元件
terminator 972..1003
/note="双向大肠杆菌tonB-P14转录终止子"
FRT complement(1067..1114)
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP介导的重组发生在8-bp核心序列TCTAGAAA中（Turan和Bode，2011）。"
ori complement(1293..1881)
/direction=LEFT
/note="高拷贝数ColE1/pMB1/pBR322/pUC复制起点"
oriT 2181..2290
/note="incP转让来源"
terminator 3541..3586
/note="大肠杆菌rrnB基因的转录终止子T1"
lac operator 5078..5094
/bound_moiety="lac repressor encoded by lacI"
/note="lac阻遏物与lac操作员结合，抑制大肠杆菌中的转录。这种抑制可以通过添加乳糖或异丙基β-D-硫代吡喃半乳糖苷（IPTG）来缓解。"
NeoR/KanR complement(5153..5947)
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="赋予对新霉素、卡那霉素和G418（遗传霉素（R））的抗性"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
AmpR promoter complement(5948..6051)
/gene="bla"
tac promoter complement(7240..7268)
/note="强大肠杆菌启动子；trp和lac UV5启动子的杂交"
FRT complement(7319..7366)
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP介导的重组发生在8-bp核心序列TCTAGAAA中（Turan和Bode，2011）。"
RBS 7458..7480
/note="噬菌体T7基因10的高效核糖体结合位点（Olins和Rangwala，1989）"
