| Plasmid Name | pCAGGS-HA-TRIM25 | Alternative Names | NM_005082;EFP;RNF147;Z147;ZNF147 |
|---|---|---|---|
| Promoter | CAG | Replicon | pUC |
| Copy Number | high | Competent Cell | DH5a |
| Culture Temp | 37°C | Forward Primer | pcaggs-F：GTTCGGCTTCTGGCGTGT |
| Reverse Primer | pcaggs-R：TATGTCCTTCCGAGTGAGAG | Storage Temp | 37°C |
1.For Research Use Only
All products available through the MiaoLing Plasmid Platform are intended for research use only. They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.
2.Plasmid Sequence and Experimental Performance
Plasmid products are provided and sold based on the corresponding reference/template plasmid. MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.
3.Sequence Verification of Certain Vector Products
For certain vector products, even the original manufacturer may not have fully verified the complete plasmid sequence. If the sequence of the key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.
4.After-Sales Period for Small-Pack Plasmid Products
The after-sales service period for small-pack plasmid products is three months from the date of receipt. Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.
5.Research-Related Risks and Limitation of Liability
Scientific research is inherently exploratory and involves a significant degree of uncertainty. To the maximum extent permitted by applicable law, MiaoLing shall not be liable for any additional economic losses, indirect losses, or other liabilities arising from the use of our products or from research outcomes.
6.Citation in Chinese Publications
For publications in Chinese, please cite the product as follows:
7.Product Name (Product Item Number) was provided by the MiaoLing Plasmid Platform.
Citation in English Publications
For publications in English, please cite the product as follows:
“Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”
CMV enhancer 4..383
/note="人类巨细胞病毒即刻早期增强子"
chicken beta-actin promoter 385..662
intron 663..1680
/note="鸡β肌动蛋白内含子与兔β珠蛋白嵌合体"
KOZAKA 1729..1734
HA 1738..1764
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/translation="YPYDVPDYA"
primer_bind 1741..1800
TRIM25 1771..3663
primer_bind complement(3634..3693)
polyA_site 3760..3815
/note="兔β-珠蛋白多聚腺苷酸化信号"
primer_bind complement(4176..4192)
/note="常见的测序引物，多种相似变体之一"
lac operator 4200..4216
/bound_moiety="lac repressor encoded by lacI"
/note="lac阻遏物与lac操作员结合，抑制大肠杆菌中的转录。这种抑制可以通过添加乳糖或异丙基β-D-硫代吡喃半乳糖苷（IPTG）来缓解。"
lac promoter complement(4224..4254)
/note="大肠杆菌lac操纵子的启动子"
CAP binding site 4269..4290
/bound_moiety="E. coli catabolite activator protein"
/note="CAP结合在cAMP存在下激活转录。"
SV40 promoter 4349..4544
/note="sv40早期启动子"
SV40 ori 4395..4530
/note="SV40复制起点"
SV40 poly(A) signal 4550..4684
/note="SV40聚腺苷酸化信号"
ori complement(4922..5510)
/direction=LEFT
/note="高拷贝数ColE1/pMB1/pBR322/pUC复制起点"
AmpR complement(5681..6541)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="对氨苄青霉素、羧苄青霉素和相关抗生素产生耐药性"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
AmpR promoter complement(6542..6646)
/gene="bla" 