| Plasmid Name | pLVX-IRES-Puro-TGFBR2 | Alternative Names | NM_003242;AAT3;FAA3;LDS1B;LDS2;LDS2B;MFS2;RIIC; TAAD2;TBR-ii;TBRII;TGFbeta-RII;TGFR-2 |
|---|---|---|---|
| Promoter | CMV | Replicon | pUC |
| Copy Number | high | Competent Cell | Stbl3 |
| Culture Temp | 37°C | Storage Temp | 37°C |
1.For Research Use Only
All products available through the MiaoLing Plasmid Platform are intended for research use only. They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.
2.Plasmid Sequence and Experimental Performance
Plasmid products are provided and sold based on the corresponding reference/template plasmid. MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.
3.Sequence Verification of Certain Vector Products
For certain vector products, even the original manufacturer may not have fully verified the complete plasmid sequence. If the sequence of the key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.
4.After-Sales Period for Small-Pack Plasmid Products
The after-sales service period for small-pack plasmid products is three months from the date of receipt. Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.
5.Research-Related Risks and Limitation of Liability
Scientific research is inherently exploratory and involves a significant degree of uncertainty. To the maximum extent permitted by applicable law, MiaoLing shall not be liable for any additional economic losses, indirect losses, or other liabilities arising from the use of our products or from research outcomes.
6.Citation in Chinese Publications
For publications in Chinese, please cite the product as follows:
7.Product Name (Product Item Number) was provided by the MiaoLing Plasmid Platform.
Citation in English Publications
For publications in English, please cite the product as follows:
“Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”
HIV-1 Psi 681..806
/note="人类免疫缺陷病毒1型包装信号"
RRE 1303..1536
/note="HIV-1的Rev反应元件（RRE）允许Rev依赖性mRNA从细胞核输出到细胞质。"
cPPT/CTS 2028..2143
/note="HIV-1的中央多泌尿道和中央终止序列（缺少第一个T）"
CMV enhancer 2201..2504
/note="人类巨细胞病毒即刻早期增强子"
CMV promoter 2505..2708
/note="人巨细胞病毒（CMV）即刻早期启动子"
primer_bind 2803..2841
regulatory 2809..2818
/regulatory_class="other"
/note="脊椎动物强启动翻译的共识序列（Kozak，1987）"
TGFBR2 2815..4518
/codon_start=1
/translation="MGRGLLRGLWPLHIVLWTRIASTIPPHVQKSVNNDMIVTDNNGAV
KFPQLCKFCDVRFSTCDNQKSCMSNCSITSICEKPQEVCVAVWRKNDENITLETVCHDP
KLPYHDFILEDAASPKCIMKEKKKPGETFFMCSCSSDECNDNIIFSEEYNTSNPDLLLV
IFQVTGISLLPPLGVAISVIIIFYCYRVNRQQKLSSTWETGKTRKLMEFSEHCAIILED
DRSDISSTCANNINHNTELLPIELDTLVGKGRFAEVYKAKLKQNTSEQFETVAVKIFPY
EEYASWKTEKDIFSDINLKHENILQFLTAEERKTELGKQYWLITAFHAKGNLQEYLTRH
VISWEDLRKLGSSLARGIAHLHSDHTPCGRPKMPIVHRDLKSSNILVKNDLTCCLCDFG
LSLRLDPTLSVDDLANSGQVGTARYMAPEVLESRMNLENVESFKQTDVYSMALVLWEMT
SRCNAVGEVKDYEPPFGSKVREHPCVESMKDNVLRDRGRPEIPSFWLNHQGIQMVCETL
TECWDHDPEARLTAQCVAERFSELEHLDRLSGRSCSEEKIPEDGSLNTTK"
WELQut site 4070..4081
/codon_start=1
/product="WELQut protease recognition and cleavage site"
Cleavage site after base 4081"
/translation="WELQ"
primer_bind complement(4486..4524)
IRES 4527..5100
/note="脑心肌炎病毒（EMCV）的内部核糖体进入位点（IRES）"
PuroR 5133..5732
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/note="赋予对嘌呤霉素的抗性"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
WPRE 5746..6334
/note="土拨鼠肝炎病毒转录后调控元件"
Factor Xa site complement(6217..6228)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
Cleavage site after base 6216"
/translation="IEGR"
3' LTR 6541..7174
/note="HIV-1的3'长末端重复序列（LTR）"
primer_bind complement(7302..7318)
/note="常见的测序引物，多种相似变体之一"
lac operator 7326..7342
/bound_moiety="lac repressor encoded by lacI"
/note="lac阻遏物与lac操作员结合，抑制大肠杆菌中的转录。这种抑制可以通过添加乳糖或异丙基β-D-硫代吡喃半乳糖苷（IPTG）来缓解。"
lac promoter complement(7350..7380)
/note="大肠杆菌lac操纵子的启动子"
CAP binding site 7395..7416
/bound_moiety="E. coli catabolite activator protein"
/note="CAP结合在cAMP存在下激活转录。"
ori complement(7704..8292)
/direction=LEFT
/note="高拷贝数ColE1/pMB1/pBR322/pUC复制起点"
AmpR complement(8463..9323)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="对氨苄青霉素、羧苄青霉素和相关抗生素产生耐药性"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
AmpR promoter complement(9324..9428)
/gene="bla"
SV40 poly(A) signal 9476..9610
/note="SV40聚腺苷酸化信号" 