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pLVX-AcGFP1-N1-FOXO3

Catalog Number P4188
Alternative Names NM_001455.3;AF6q21;FKHRL1;FKHRL1P2;FOXO2;FOXO3A
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Product Information

Product Overview

Instructions Ⅰ. Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature) 1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid; 2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min; 3. 42 degrees heat shock for 60s, ice bath for 2min, add 900μl of antibiotic-free LB medium, and culture at 180rpm shaking for 45min; 4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate; 5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation; 6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed. Ⅱ. Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation). 1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall; 2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer. Ⅲ. Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation). 1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall; 2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance; 3. Inverted culture in constant temperature incubator for 12~16h; 4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance; 5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.

Product Disclaimer

  1. For Research Use Only
    All products available through the MiaoLing Plasmid Platform are intended for research use only. They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.
  2. Plasmid Sequence and Experimental Performance
    Plasmid products are provided and sold based on the corresponding reference/template plasmid. MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.
  3. Sequence Verification of Certain Vector Products
    For certain vector products, even the original manufacturer may not have fully verified the complete plasmid sequence. If the sequence of the key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.
  4. After-Sales Period for Small-Pack Plasmid Products
    The after-sales service period for small-pack plasmid products is three months from the date of receipt. Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.
  5. Research-Related Risks and Limitation of Liability
    Scientific research is inherently exploratory and involves a significant degree of uncertainty. To the maximum extent permitted by applicable law, MiaoLing shall not be liable for any additional economic losses, indirect losses, or other liabilities arising from the use of our products or from research outcomes.
  6. Citation in Chinese Publications
    For publications in Chinese, please cite the product as follows:
    Product names and IDs are provided by the MiaoLing Plasmid Platform.
  7. Citation in English Publications
    For publications in English, please cite the product as follows:
    “Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”

Related Publications

1、 Beini Sun;Hongce Chen;Xiaoping Wang;Tongsheng Chen,Regorafenib induces Bim-mediated intrinsic apoptosis by blocking AKT-mediated FOXO3a nuclear export,2023-1-1,DOI:10.1038/s41420-023-01338-9,(IF:6.1)
2、 Beini Sun;Hongce Chen;Xiaoping Wang;Tongsheng Chen,Regorafenib induces Bim-mediated intrinsic apoptosis by blocking AKT-mediated FOXO3a nuclear export,2023-1-1,DOI:10.1038/s41420-023-01338-9,(IF:6.1)

Product Documents

P4188_Full Sequence Map.dna P4188_Sequencing report.zip

Plasmid Elements

HIV-1 Psi 681..806 /note="人类免疫缺陷病毒1型包装信号" RRE 1303..1536 /note="HIV-1的Rev反应元件（RRE）允许Rev依赖性mRNA从细胞核输出到细胞质。" cPPT/CTS 2028..2143 /note="HIV-1的中央多泌尿道和中央终止序列（缺少第一个T）" CMV enhancer 2201..2504 /note="人类巨细胞病毒即刻早期增强子" CMV promoter 2505..2708 /note="人巨细胞病毒（CMV）即刻早期启动子" FOXO3 2826..4844 /codon_start=1 /translation="MAEAPASPAPLSPLEVELDPEFEPQSRPRSCTWPLQRPELQASPA KPSGETAADSMIPEEEDDEDDEDGGGRAGSAMAIGGGGGSGTLGSGLLLEDSARVLAPG GQDPGSGPATAAGGLSGGTQALLQPQQPLPPPQPGAAGGSGQPRKCSSRRNAWGNLSYA DLITRAIESSPDKRLTLSQIYEWMVRCVPYFKDKGDSNSSAGWKNSIRHNLSLHSRFMR VQNEGTGKSSWWIINPDGGKSGKAPRRRAVSMDNSNKYTKSRGRAAKKKAALQTAPESA DDSPSQLSKWPGSPTSRSSDELDAWTDFRSRTNSNASTVSGRLSPIMASTELDEVQDDD APLSPMLYSSSASLSPSVSKPCTVELPRLTDMAGTMNLNDGLTENLMDDLLDNITLPPS QPSPTGGLMQRSSSFPYTTKGSGLGSPTSSFNSTVFGPSSLNSLRQSPMQTIQENKPAT FSSMSHYGNQTLQDLLTSDSLSHSDVMMTQSDPLMSQASTAVSAQNSRRNVMLRNDPMM SFAAQPNQGSLVNQNLLHHQHQTQGALGGSRALSNSVSNMGLSESSSLGSAKHQQQSPV SQSMQTLSDSLSGSSLYSTSANLPVMGHEKFPSDLDLDMFNGSLECDMESIIRSELMDA DGLDFNFDSLISTQNVVGLNVGNFTGAKQASSQSWVPG" AcGFP1 4860..5579 /codon_start=1 /product="Aequorea coerulescens GFP" /note="哺乳动物密码子优化" /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF GFVTAAAITHGMDELYK" PGK promoter 5607..6106 /note="小鼠磷酸甘油酸激酶1启动子" primer_bind complement(5698..5718) PuroR 6127..6726 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /note="赋予对嘌呤霉素的抗性" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" WPRE 6740..7328 /note="土拨鼠肝炎病毒转录后调控元件" 3' LTR 7535..8168 /note="HIV-1的3'长末端重复序列（LTR）" ori complement(8699..9287) /direction=LEFT /note="高拷贝数ColE1/pMB1/pBR322/pUC复制起点" AmpR complement(9458..10318) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="对氨苄青霉素、羧苄青霉素和相关抗生素产生耐药性" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" AmpR promoter complement(10319..10423) /gene="bla" SV40 poly(A) signal 10471..10605 /note="SV40聚腺苷酸化信号"
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