pBARGPE1-EGFP-BenA载体质粒图谱、序列、抗性、价格、测序引物、大小等信息_淼灵质粒平台 - Plasmids in Stock

基本信息

产品简介

产品声明

For Research Use Only
All products available through the MiaoLing Plasmid Platform are intended for research use only. They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.
Plasmid Sequence and Experimental Performance
Plasmid products are provided and sold based on the corresponding reference/template plasmid. MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.
Sequence Verification of Certain Vector Products
For certain vector products, even the original manufacturer may not have fully verified the complete plasmid sequence. If the sequence of the key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.
After-Sales Period for Small-Pack Plasmid Products
The after-sales service period for small-pack plasmid products is three months from the date of receipt. Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.
Research-Related Risks and Limitation of Liability
Scientific research is inherently exploratory and involves a significant degree of uncertainty. To the maximum extent permitted by applicable law, MiaoLing shall not be liable for any additional economic losses, indirect losses, or other liabilities arising from the use of our products or from research outcomes.
Citation in Chinese Publications
For publications in Chinese, please cite the product as follows:
产品名称（产品货号）由淼灵质粒平台提供。
Citation in English Publications
For publications in English, please cite the product as follows:
“Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”

质粒序列

GGTACCGGGCCCCCCCTCGAGGTCGACGGTATCGATAAGCTTGATATCGAATTCTTACTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTCACGAACTCCAGCAGGACCATGTGATCGCGCTTCTCGTTGGGGTCTTTGCTCAGGGCGGACTGGGTGCTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGCTGGTAGTGGTCGGCGAGCTGCACGCTGCCGTCCTCGATGTTGTGGCGGATCTTGAAGTTCACCTTGATGCCGTTCTTCTGCTTGTCGGCCATGATATAGACGTTGTGGCTGTTGTAGTTGTACTCCAGCTTGTGCCCCAGGATGTTGCCGTCCTCCTTGAAGTCGATGCCCTTCAGCTCGATGCGGTTCACCAGGGTGTCGCCCTCGAACTTCACCTCGGCGCGGGTCTTGTAGTTGCCGTCGTCCTTGAAGAAGATGGTGCGCTCCTGGACGTAGCCTTCGGGCATGGCGGACTTGAAGAAGTCGTGCTGCTTCATGTGGTCGGGGTAGCGGCTGAAGCACTGCACGCCGTAGGTCAGGGTGGTCACGAGGGTGGGCCAGGGCACGGGCAGCTTGCCGGTGGTGCAGATGAACTTCAGGGTCAGCTTGCCGTAGGTGGCATCGCCCTCGCCCTCGCCGGACACGCTGAACTTGTGGCCGTTTACGTCGCCGTCCAGCTCGACCAGGATGGGCACCACCCCGGTGAACAGCTCCTCGCCCTTGCTCACCATGGATCCTCTAGAGTCGAGGTCGACGGTATCGATAAGCTTGATTAAAGGTTCTTGGATGGGAAGATGAATATACTGAAGATGGGAAAAGAAAGAGAAAAGAAAAGAGCAGCTGGTGGGGAGAGCAGGAAAATATGGCAACAAATGTTGGACTGACGCAACGACCTTGTCAACCCCGCCGACACACCGGGCGGACAGACGGGGCAAAGCTGCCTACCAGGGACTGAGGGACCTCAGCAGGTCGAGTGCAGAGCACCGGATGGGTCGACTGCCAGCTTGTGTTCCCGGTCTGCGCCGCTGGCCAGCTCCTGAGCGGCCTTTCCGGTTTCATACACCGGGCAAAGCAGGAGAGGCACGATATTTGGACGCCCTACAGATGCCGGATGGGCCAATTAGGGAGCTTACGCGCCGGGTACTCGCTCTACCTACTTCGGAGAAGGTACTATCTCGTGAATCTTTTACCAGATCGGAAGCAATTGGACTTCTGTACCTAGGTTAATGGCATGCTATTTCGCCGACGGCTATACACCCCTGGCTTCACATTCTCCTTCGCTTACTGCCGGTGATTCGATGAAGCTCCATATTCTCCGATGATGCAATAGATTCTTGGTCAACGAGGGGCACACCAGCCTTTCCACTTCGGGGCGGAGGGGCGGCCGGTCCCGGATTAATAATCATCCACTGCACCTCAGAGCCGCCAGAGCTGTCTGGCGCAGTGGCGCTTATTACTCAGCCCTTCTCTCTGCGTCCGTCCGTCTCTCCGCATGCTTTGCATACTTCTGCCTGCTGGGGAGCCTGGGGACTTTCCACACCCTAACTGACACACATTCCACAGAGATCTCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAATTAATTCGGTCGAAAAAAGAAAAGGAGAGGGCCAAGAGGGAGGGCATTGGTGACTATTGAGCACGTGAGTATACGTGATTAAGCACACAAAGGCAGCTTGGAGTATGTCTGTTATTAATTTCACAGGTAGTTCTGGTCCATTGGTGAAAGTTTGCGGCTTGCAGAGCACAGAGGCCGCAGAATGTGCTCTAGACTCGACAGAAGATGATATTGAAGGAGCACTTTTTGGGCTTGGCTGGAGCTAGTGGAGGTCAACAATGAATGCCTATTTTGGTTTAGTCGTCCAGGCGGTGAGCACAAAATTTGTGTCGTTTGACAAGATGGTTCATTTAGGCAACTGGTCAGATCAGCCCCACTTGTAGCAGTAGCGGCGGCGCTCGAAGTGTGACTCTTATTAGCAGACAGGAACGAGGACATTATTATCATCTGCTGCTTGGTGCACGATAACTTGGTGCGTTTGTCAAGCAAGGTAAGTGAACGACCCGGTCATACCTTCTTAAGTTCGCCCTTCCTCCCTTTATTTCAGATTCAATCTGACTTACCTATTCTACCCAAGCAAAGCTTCGATTAGGAAGTAACCATGCGTGAGATTGTCCATCTTCAAACCGGCCAATGTGGTAACCAAATTGGTGCTGCTTTCTGGCAAACTATCTCTGGCGAGCACGGTCTTGACGGTTCCGGTGTCTATAATGGTACATCCGATCTCCAACTTGAGCGTATGAACGTCTACTTCAACGAGGCTTCTGGCAACAAGTATGTTCCCCGTGCCGTCCTCGTCGATTTGGAGCCAGGTACCATGGATGCTGTCCGTGCCGGTCCTTTCGGTCAACTCTTCCGTCCCGACAACTTCGTTTTCGGTCAATCTGGTGCTGGTAACAACTGGGCTAAGGGTCATTACACTGAGGGTGCTGAGCTTGTCGACCAAGTTCTTGATGTTGTCCGTCGTGAAGCTGAAGGCTGTGACTGCCTTCAAGGATTCCAAATTACCCACTCTCTCGGTGGTGGAACTGGTGCCGGTATGGGTACGCTTTTGATCTCCAAGATCCGCGAGGAGTTCCCAGATCGTATGATGGCTACCTTCTCCGTCGTCCCATCGCCAAAGGTTTCCGATACCGTTGTCGAGCCATATAACGCAACTCTCTCTGTCCATCAATTGGTTGAGAACTCTGACGCGACCTTCTGTATCGATAACGAGGCTCTTTACGATATTTGCATGAGAACCTTGAAGCTCAGCAACCCATCTTACGGAGATCTTAACCACTTGGTTTCCGCCGTCATGTCCGGTGTTACCACCTGTCTCCGTTTCCCTGGTCAACTTAACTCAGATCTCCGAAAGTTGGCTGTTAACATGGTTCCATTCCCCCGTCTCCATTTCTTCATGGTTGGATTTGCTCCTTTGACCAGTCGTGGCGCACACTCTTTCCGTGCTGTCACCGTTCCAGAGTTGACTCAACAAATGTACGACCCTAAGAACATGATGGCCGCTTCCGATTTCCGTAACGGTCGTTACTTGACATGCTCTGCCATTTTCCGTGGTAAGGTTTCCATGAAGGAGGTTGAGGACCAAATGCGCAACGTCCAAAACAAGAACTCATCCTACTTCGTTGAGTGGATCCCTAACAACGTCCAAACCGCCCTTTGCTCCATTCCTCCCCGTGGTCTCAAGATGTCCTCCACCTTCGTTGGTAACTCGACATCCATCCAAGAACTTTTCAAGCGTGTCGGTGATCAATTCACTGCTATGTTCAGAAGAAAGGCTTTCTTGCATTGGTACACTGGTGAAGGTATGGACGAGATGGAGTTCACTGAGGCTGAGTCCAACATGAACGATTTGGTTTCCGAGTATCAACAATACCAGGATGCCTCGATCTCTGAGGGAGAGGAGGAGTACGAAGAGGAGGTCCCAATTGAGGGCGAGGAATAGTCCGTCGACCTGCAGATCGTTCAAACATTTGGCAATAAAGTTTCTTAAGATTGAATCCTGTTGCCGGTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATGTAATGCATAGTACCGAGAAACTAGTGGATCTAAACATAAAATCTGTAAAATAACAAGATGTAAAGATAATGCTAAATCATTTGGCTTTTTGATTGATTGTACAGGAAAATATACATCGCAGGGGGTTGACTTTTACCATTTCACCGCAATGGAATCAAACTTGTTGAAGAGAATGTTCACAGGCGCATACGCTACAATGACCCGATTCTTGCTAGCCTTTTCTCGGTCTTGCAAACAACCGCCGCGGCCGGTCGAGGGACCTAATAACTTCGTATAGCATACATTATACGAAGTTATATTAAGGGTTCCGGATCGCGGCCGCTCGACCTGCAGCCAAGCTAGCTTGGCTGGACGTAAACTCCTCTTCAGACCTAATAACTTCGTATAGCATACATTATACGAAGTTATATTAAGGGTTATTGAATATGATCGGAATTGGTCGATCAGGTATTGCTGTTATCTGATGAGTATACGTTGTCCTGGCCACGGCAGAAGCACGCTTATCGCTCCAATTTCCCACAACATTAGTCAACTCCGTTAGGCCCTTCATTGAAAGAAATGAGGTCATCAAATGTCTTCCAATGTGAGATTTTGGGCCATTTTTTATAGCAAAGATTGAATAAGGCGCATTTTTCTTCAAAGCTTGCAAATTAAAGCCTTCGAGCGTCCCAAAACCTTCTCAAGCAAGGTTTTCAGTATAATGTTACATGCGTACACGCGCTATATTTTGTTTTCTATCGCGTATTAAATGTATAATTGCGGGACTCTAATCATAAAAACCCATCTCATAAATAACGTCATGCATTGCAGATGAGCTGTATCTGGAAGAGGTAAACCCGAAACGCGTTTTATTCTTGTTGACATGGAGCTATTAAATCACTAGAAGGCACTCTTTGCTGCTTGGACAAATGAACGTATCTTATCGAGATCCTGAACACCATTTGTCTCAACTCCGGAGCTGACATCGACACCAACGATCTTATATCCAGATTCGTCAAGCTGTTTGATGATTTCAGTAACGTTAAGTGGATCTCGGTGACGGGCAGGACCGGACGGGGC

产品文件

P3476_测序报告.zip P3476_全序列图谱.dna

质粒元件

                                                            
     primer_bind     18..34
                     /note="常见的测序引物，多种相似变体之一"
     EGFP             complement(55..774)
                     /codon_start=1
                     /product="enhanced GFP"
                     /note="哺乳动物密码子优化"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     789..805
                     /note="常见的测序引物，多种相似变体之一"
     bom    1754..1894
                     /note="pBR322迁移区的基础"
     ori      complement(2080..2668)
                     /direction=LEFT
                     /note="高拷贝数ColE1/pMB1/pBR322/pUC复制起点"
     AmpR             complement(2839..3699)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="对氨苄青霉素、羧苄青霉素和相关抗生素产生耐药性"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     AmpR promoter        complement(3700..3804)
                     /gene="bla"
     TRP1 promoter        3910..4011
                     /gene="S. cerevisiae TRP1"
     BenA             4488..5831
                     /codon_start=1
                     /translation="MREIVHLQTGQCGNQIGAAFWQTISGEHGLDGSGVYNGTSDLQLE
                     RMNVYFNEASGNKYVPRAVLVDLEPGTMDAVRAGPFGQLFRPDNFVFGQSGAGNNWAKG
                     HYTEGAELVDQVLDVVRREAEGCDCLQGFQITHSLGGGTGAGMGTLLISKIREEFPDRM
                     MATFSVVPSPKVSDTVVEPYNATLSVHQLVENSDATFCIDNEALYDICMRTLKLSNPSY
                     GDLNHLVSAVMSGVTTCLRFPGQLNSDLRKLAVNMVPFPRLHFFMVGFAPLTSRGAHSF
                     RAVTVPELTQQMYDPKNMMAASDFRNGRYLTCSAIFRGKVSMKEVEDQMRNVQNKNSSY
                     FVEWIPNNVQTALCSIPPRGLKMSSTFVGNSTSIQELFKRVGDQFTAMFRRKAFLHWYT
                     GEGMDEMEFTEAESNMNDLVSEYQQYQDASISEGEEEYEEEVPIEGEE"
     loxP    6223..6256
                     /bound_moiety="Cre recombinase"
                     /note="Cre介导的重组发生在8-bp核心序列（GCATACAT）中。"
     loxP    6334..6367
                     /bound_moiety="Cre recombinase"
                     /note="Cre介导的重组发生在8-bp核心序列（GCATACAT）中。"                             
                        

pBARGPE1-EGFP-BenA

基本信息

产品简介

产品声明

质粒序列

相关论文

产品文件

质粒元件

质粒序列