产品声明
For Research Use Only research use only . They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.Plasmid Sequence and Experimental Performance reference/template plasmid . MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.Sequence Verification of Certain Vector Products key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.After-Sales Period for Small-Pack Plasmid Products three months from the date of receipt . Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.Research-Related Risks and Limitation of Liability Citation in Chinese Publications 产品名称（产品货号）由淼灵质粒平台提供。 Citation in English Publications “Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”
质粒序列
一键复制序列（有部分质粒是非全长序列）
TAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTGTAGAAATAATTTTGTTTAACTTTAATAAGGAGATATACCATGGCAAGCTGGTCACATCCGCAGTTTGAAAAAGGTGCACTGGAAGTTCTGTTTCAGGGTCCTGGGTACCAGATGAAAGCAAAAGTTGTTATTAACCTGGTGAAAATCAACAAAAAAATCATCCCGGATAAAATCTACGTGTACCGCCTGTTTAATGATCCGGAAGAAGAACTGCAGAAAGAAGGCTATAGCATTTATCGTCTGGCCTATGAAAATGTGGGCATTGTTATCGATCCGGAAAATCTGATTATTGCCACCACCAAAGAACTGGAATATGAAGGTGAATTTATTCCGGAAGGCGAAATCAGCTTTAGCGAACTGCGTAATGATTATCAGAGCAAACTGGTTCTGCGTCTGCTGAAAGAAAATGGTATTGGTGAATATGAACTGTCCAAACTGCTGCGCAAATTTCGTAAACCGAAAACCTTTGGCGATTACAAAGTTATTCCGAGCGTTGAAATGAGCGTGATCAAACACGATGAAGATTTCTATCTGGTGATCCATATCATCCATCAGATCCAGAGCATGAAAACCCTGTGGGAACTGGTTAATAAAGATCCGAAAGAGCTGGAAGAATTTCTGATGACCCATAAAGAAAACCTGATGCTGAAAGATATTGCCAGTCCGCTGAAAACCGTGTATAAACCGTGTTTTGAAGAATATACCAAAAAACCGAAACTGGACCACAACCAAGAGATCGTGAAATATTGGTATAACTATCACATCGAGCGCTATTGGAATACACCGGAAGCAAAACTGGAATTCTATCGCAAATTTGGTCAGGTGGATCTGAAACAGCCTGCAATTCTGGCAAAATTTGCAAGCAAAATTAAAAAAAATAAAAACTACAAAATCTATCTGCTGCCGCAGCTGGTTGTTCCGACCTATAATGCAGAACAGCTGGAAAGTGATGTGGCCAAAGAAATTCTGGAATATACAAAACTGATGCCTGAGGAACGTAAAGAGCTGCTGGAAAATATTCTGGCAGAAGTGGATAGCGATATCATCGATAAAAGCCTGAGCGAAATCGAGGTTGAAAAAATTGCACAAGAACTGGAAAACAAAATCCGCGTGCGTGATGATAAAGGTAATAGCGTTCCGATTAGCCAGCTGAATGTTCAGAAAAGCCAGCTGCTGCTGTGGACCAATTATTCACGTAAATATCCGGTTATCCTGCCGTATGAAGTGCCGGAAAAATTTCGCAAAATTCGTGAAATCCCGATGTTCATTATTCTGGATAGCGGTCTGCTGGCAGATATTCAGAACTTTGCAACCAATGAATTTCGCGAGCTGGTCAAAAGCATGTATTATAGCCTGGCCAAAAAATACAACTCCCTGGCAAAAAAAGCACGCAGCACCAATGAAATTGGTCTGCCGTTTCTGGATTTTCGCGGTAAAGAAAAAGTGATCACCGAAGATCTGAATAGCGATAAAGGCATTATTGAAGTTGTTGAACAGGTGAGCAGCTTTATGAAAGGTAAAGAACTGGGTCTGGCATTTATTGCAGCACGTAATAAACTGAGCAGCGAGAAATTTGAAGAAATCAAACGCCGTCTGTTTAACCTGAATGTTATTAGCCAGGTGGTGAATGAAGATACCCTGAAAAACAAACGCGATAAATATGATCGTAATCGCCTGGACCTGTTTGTTCGTCATAATCTGCTGTTCCAGGTTCTGAGTAAACTGGGTGTTAAATACTATGTGCTGGACTATCGCTTCAACTACGATTATATCATTGGCATTGATGTGGCACCGATGAAACGTAGCGAAGGTTATATTGGTGGTAGCGCAGTTATGTTTGATAGCCAGGGTTATATTCGTAAAATCGTGCCGATTAAAATCGGTGAACAGCGTGGTGAAAGCGTTGATATGAACGAATTTTTCAAAGAAATGGTGGACAAATTCAAAGAGTTCAACATCAAACTGGATAACAAAAAAATCCTGCTGCTGCGTGATGGTCGCATTACCAATAATGAAGAAGAAGGCCTGAAATATATCAGCGAGATGTTCGATATTGAAGTGGTGACAATGGATGTGATCAAAAACCATCCGGTTCGTGCATTTGCAAACATGAAAATGTATTTTAACCTGGGTGGTGCCATTTATCTGATTCCGCATAAACTGAAACAGGCAAAAGGCACCCCGATTCCGATTAAACTGGCGAAAAAACGCATTATCAAAAACGGCAAAGTGGAAAAACAGAGCATTACCCGTCAGGATGTTCTGGATATCTTTATTCTGACCCGTCTGAATTATGGTAGCATTAGCGCAGATATGCGTCTGCCTGCACCGGTTCATTATGCACATAAATTTGCCAATGCCATTCGCAACGAGTGGAAAATCAAAGAAGAATTCCTGGCCGAAGGCTTTCTGTATTTTGTGTAATAACCTAGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAACCTCAGGCATTTGAGAAGCACACGGTCACACTGCTTCCGGTAGTCAATAAACCGGTAAACCAGCAATAGACATAAGCGGCTATTTAACGACCCTGCCCTGAACCGACGACCGGGTCATCGTGGCCGGATCTTGCGGCCCCTCGGCTTGAACGAATTGTTAGACATTATTTGCCGACTACCTTGGTGATCTCGCCTTTCACGTAGTGGACAAATTCTTCCAACTGATCTGCGCGCGAGGCCAAGCGATCTTCTTCTTGTCCAAGATAAGCCTGTCTAGCTTCAAGTATGACGGGCTGATACTGGGCCGGCAGGCGCTCCATTGCCCAGTCGGCAGCGACATCCTTCGGCGCGATTTTGCCGGTTACTGCGCTGTACCAAATGCGGGACAACGTAAGCACTACATTTCGCTCATCGCCAGCCCAGTCGGGCGGCGAGTTCCATAGCGTTAAGGTTTCATTTAGCGCCTCAAATAGATCCTGTTCAGGAACCGGATCAAAGAGTTCCTCCGCCGCTGGACCTACCAAGGCAACGCTATGTTCTCTTGCTTTTGTCAGCAAGATAGCCAGATCAATGTCGATCGTGGCTGGCTCGAAGATACCTGCAAGAATGTCATTGCGCTGCCATTCTCCAAATTGCAGTTCGCGCTTAGCTGGATAACGCCACGGAATGATGTCGTCGTGCACAACAATGGTGACTTCTACAGCGCGGAGAATCTCGCTCTCTCCAGGGGAAGCCGAAGTTTCCAAAAGGTCGTTGATCAAAGCTCGCCGCGTTGTTTCATCAAGCCTTACGGTCACCGTAACCAGCAAATCAATATCACTGTGTGGCTTCAGGCCGCCATCCACTGCGGAGCCGTACAAATGTACGGCCAGCAACGTCGGTTCGAGATGGCGCTCGATGACGCCAACTACCTCTGATAGTTGAGTCGATACTTCGGCGATCACCGCTTCCCTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGCTAGCTCACTCGGTCGCTACGCTCCGGGCGTGAGACTGCGGCGGGCGCTGCGGACACATACAAAGTTACCCACAGATTCCGTGGATAAGCAGGGGACTAACATGTGAGGCAAAACAGCAGGGCCGCGCCGGTGGCGTTTTTCCATAGGCTCCGCCCTCCTGCCAGAGTTCACATAAACAGACGCTTTTCCGGTGCATCTGTGGGAGCCGTGAGGCTCAACCATGAATCTGACAGTACGGGCGAAACCCGACAGGACTTAAAGATCCCCACCGTTTCCGGCGGGTCGCTCCCTCTTGCGCTCTCCTGTTCCGACCCTGCCGTTTACCGGATACCTGTTCCGCCTTTCTCCCTTACGGGAAGTGTGGCGCTTTCTCATAGCTCACACACTGGTATCTCGGCTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTAAGCAAGAACTCCCCGTTCAGCCCGACTGCTGCGCCTTATCCGGTAACTGTTCACTTGAGTCCAACCCGGAAAAGCACGGTAAAACGCCACTGGCAGCAGCCATTGGTAACTGGGAGTTCGCAGAGGATTTGTTTAGCTAAACACGCGGTTGCTCTTGAAGTGTGCGCCAAAGTCCGGCTACACTGGAAGGACAGATTTGGTTGCTGTGCTCTGCGAAAGCCAGTTACCACGGTTAAGCAGTTCCCCAACTGACTTAACCTTCGATCAAACCACCTCCCCAGGTGGTTTTTTCGAAGGTTAAGTCAGTTGGGGAACTGCTTAACCTTCGATCAAACCACCTCCCCAGGTGGTTTTTTCGTTTACAGGGCAAAAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACTGAACCGCTCTAGATTTCAGTGCAATTTATCTCTTCAAATGTAGCACCTGAAGTCAGCCCCATACGATATAAGTTGTAATTCTCATGTTAGTCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCTCAAGGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATGTCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCGCGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTATGCGACTCCTGCATTAGGAAAT
质粒元件
T7 promoter 1..19
/note="噬菌体T7 RNA聚合酶启动子"
lac operator 20..44
/bound_moiety="lac repressor encoded by lacI"
/note="lac阻遏物与lac操作员结合，抑制大肠杆菌中的转录。这种抑制可以通过添加乳糖或异丙基β-D-硫代吡喃半乳糖苷（IPTG）来缓解。"
CDS 97..120
/codon_start=1
/product="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
/translation="WSHPQFEK"
CDS 127..150
/codon_start=1
/product="recognition and cleavage site for human
rhinovirus 3C and PreScission proteases"
Cleavage site after base 144"
/translation="LEVLFQGP"
PfAgo 160..2472
/codon_start=1
/product="Argonaute protein from the archaeon Pyrococcus
furiosus"
/note="DNA引导核酸酶（Swarts等人，2015）"
/translation="MKAKVVINLVKINKKIIPDKIYVYRLFNDPEEELQKEGYSIYRLA
YENVGIVIDPENLIIATTKELEYEGEFIPEGEISFSELRNDYQSKLVLRLLKENGIGEY
ELSKLLRKFRKPKTFGDYKVIPSVEMSVIKHDEDFYLVIHIIHQIQSMKTLWELVNKDP
KELEEFLMTHKENLMLKDIASPLKTVYKPCFEEYTKKPKLDHNQEIVKYWYNYHIERYW
NTPEAKLEFYRKFGQVDLKQPAILAKFASKIKKNKNYKIYLLPQLVVPTYNAEQLESDV
AKEILEYTKLMPEERKELLENILAEVDSDIIDKSLSEIEVEKIAQELENKIRVRDDKGN
SVPISQLNVQKSQLLLWTNYSRKYPVILPYEVPEKFRKIREIPMFIILDSGLLADIQNF
ATNEFRELVKSMYYSLAKKYNSLAKKARSTNEIGLPFLDFRGKEKVITEDLNSDKGIIE
VVEQVSSFMKGKELGLAFIAARNKLSSEKFEEIKRRLFNLNVISQVVNEDTLKNKRDKY
DRNRLDLFVRHNLLFQVLSKLGVKYYVLDYRFNYDYIIGIDVAPMKRSEGYIGGSAVMF
DSQGYIRKIVPIKIGEQRGESVDMNEFFKEMVDKFKEFNIKLDNKKILLLRDGRITNNE
EEGLKYISEMFDIEVVTMDVIKNHPVRAFANMKMYFNLGGAIYLIPHKLKQAKGTPIPI
KLAKKRIIKNGKVEKQSITRQDVLDIFILTRLNYGSISADMRLPAPVHYAHKFANAIRN
EWKIKEEFLAEGFLYFV"
terminator 2505..2552
/note="噬菌体T7 RNA聚合酶的转录终止子"
SmR complement(2723..3514)
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/note="赋予对壮观霉素和链霉素的抗性"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
AmpR promoter complement(3515..3606)
/gene="bla"
lacI complement(4667..5749)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/note="lac阻遏物与lac操作员结合，抑制大肠杆菌中的转录。这种抑制可以通过添加乳糖或异丙基β-D-硫代吡喃半乳糖苷（IPTG）来缓解。"
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
lacI promoter complement(5750..5827)
/gene="lacI"
