产品声明
- For Research Use Only
All products available through the MiaoLing Plasmid Platform are intended for research use only. They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo. - Plasmid Sequence and Experimental Performance
Plasmid products are provided and sold based on the corresponding reference/template plasmid. MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes. - Sequence Verification of Certain Vector Products
For certain vector products, even the original manufacturer may not have fully verified the complete plasmid sequence. If the sequence of the key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes. - After-Sales Period for Small-Pack Plasmid Products
The after-sales service period for small-pack plasmid products is three months from the date of receipt. Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement. - Research-Related Risks and Limitation of Liability
Scientific research is inherently exploratory and involves a significant degree of uncertainty. To the maximum extent permitted by applicable law, MiaoLing shall not be liable for any additional economic losses, indirect losses, or other liabilities arising from the use of our products or from research outcomes. - Citation in Chinese Publications
For publications in Chinese, please cite the product as follows:
产品名称（产品货号）由淼灵质粒平台提供。 - Citation in English Publications
For publications in English, please cite the product as follows:
“Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”
质粒元件
traJ complement(145..516)
/codon_start=1
/gene="traJ"
/product="oriT-recognizing protein"
/translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
KQDELGKVMMGVVRPRAEP"
oriT 549..658
/note="incP转让来源"
TetR complement(1339..1989)
/codon_start=1
/gene="tetR"
/product="tetracycline resistance regulatory protein"
/translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
RB T-DNA repeat 2454..2478
/note="胭脂碱C58 T-DNA的右边界重复序列"
NOS promoter 2602..2781
/note="胭脂碱合酶启动子"
NOS promoter 2634..2817
/function="NOS promoter"
/note="胭脂碱合酶启动子"
NeoR/KanR 2838..3632
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="赋予对新霉素、卡那霉素和G418（遗传霉素（R））的抗性"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
terminator 4022..4274
/note="胭脂碱合酶终止子和poly（A）信号"
CAP binding site 4823..4844
/bound_moiety="E. coli catabolite activator protein"
/note="CAP结合在cAMP存在下激活转录。"
lac promoter 4859..4889
/note="大肠杆菌lac操纵子的启动子"
/note="此正向特征有3个分段：1:4859。.4864/#fffff/-35 2:4865。.4882/#fffff 3:4883。.4889/#fffff/-10"
lac operator 4897..4913
/bound_moiety="lac repressor encoded by lacI"
/note="lac阻遏物与lac操作员结合，抑制大肠杆菌中的转录。这种抑制可以通过添加乳糖或异丙基β-D-硫代吡喃半乳糖苷（IPTG）来缓解。"
primer_bind 4921..4937
/note="常见的测序引物，多种相似变体之一"
CaMV 35S promoter 5461..5806
/note="花椰菜花叶病毒强组成型启动子"
Myc 5842..5871
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/translation="EQKLISEEDL"
Myc 5878..5907
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/translation="EQKLISEEDL"
Myc 5923..5952
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/translation="EQKLISEEDL"
Myc 5959..5988
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/translation="EQKLISEEDL"
Myc 5995..6024
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/translation="EQKLISEEDL"
Myc 6040..6069
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/translation="EQKLISEEDL"
Myc 6076..6105
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/translation="EQKLISEEDL"
Myc 6112..6141
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/translation="EQKLISEEDL"
Myc 6157..6186
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/translation="EQKLISEEDL"
Myc 6193..6222
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/translation="EQKLISEEDL"
attR1 6241..6365
/gene="mutant version of attR"
/bound_moiety="LR Clonase(TM)"
/note="Gateway（R）LR反应的重组位点"
lac UV5 promoter 6402..6432
/note="大肠杆菌lac启动子""up"" mutation"
CmR 6486..7145
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/note="赋予对氯霉素的抗性"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ccdB 7487..7792
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/note="含有ccdB基因的质粒不能在标准大肠杆菌菌株中繁殖。"
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
attR2 complement(7833..7957)
/gene="mutant version of attR"
/bound_moiety="LR Clonase(TM)"
/note="Gateway（R）LR反应的重组位点"
terminator 7983..8235
/note="胭脂碱合酶终止子和poly（A）信号"
terminator 8664..8916
/note="胭脂碱合酶终止子和poly（A）信号"
HygR complement(9384..10409)
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/note="赋予对潮霉素的抗性"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
E"
CaMV 35S promoter complement(10419..10763)
/note="花椰菜花叶病毒强组成型启动子"
primer_bind complement(10873..10889)
/note="常见的测序引物，多种相似变体之一"
LB T-DNA repeat 11507..11531
/note="胭脂碱C58 T-DNA的左边界重复序列"
CDS complement(13127..14275)
/codon_start=1
/product="trans-acting replication protein that binds to
and activates oriV"
/translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
KanR complement(14574..15368)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/note="赋予对卡那霉素的抗性"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
mobile_element 15560..16327
/mobile_element_type="insertion sequence:IS1"
/note="原核转座因子"
oriV join(16950..17643,1..18)
/note="incP复制起源"
