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pMSCV-FLIP-Puro-dsRed-GFP-miRNA

产品货号 P2287
产品别称 等效质粒Plasmid#32704
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价格: ¥580.00
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产品简介

产品声明

  1. For Research Use Only
    All products available through the MiaoLing Plasmid Platform are intended for research use only. They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.
  2. Plasmid Sequence and Experimental Performance
    Plasmid products are provided and sold based on the corresponding reference/template plasmid. MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.
  3. Sequence Verification of Certain Vector Products
    For certain vector products, even the original manufacturer may not have fully verified the complete plasmid sequence. If the sequence of the key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.
  4. After-Sales Period for Small-Pack Plasmid Products
    The after-sales service period for small-pack plasmid products is three months from the date of receipt. Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.
  5. Research-Related Risks and Limitation of Liability
    Scientific research is inherently exploratory and involves a significant degree of uncertainty. To the maximum extent permitted by applicable law, MiaoLing shall not be liable for any additional economic losses, indirect losses, or other liabilities arising from the use of our products or from research outcomes.
  6. Citation in Chinese Publications
    For publications in Chinese, please cite the product as follows:
    产品名称(产品货号)由淼灵质粒平台提供。
  7. Citation in English Publications
    For publications in English, please cite the product as follows:
    “Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”

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质粒元件

MESV Psi 580..921 /note="小鼠胚胎干细胞病毒的包装信号" gag (truncated) 988..1404 /note="缺失起始密码子的截短型莫洛尼小鼠白血病病毒gag基因" lox2272 1417..1450 /bound_moiety="Cre recombinase" /note="Cre介导的重组发生在8-bp核心序列(GGATACT)中。lox2272位点彼此兼容,但与loxP或loxN位点不兼容。" loxN 1417..1450 /bound_moiety="Cre recombinase" /note="Cre介导的重组发生在8-bp核心序列(GTATACCT)中。loxN位点彼此兼容,但与loxP或lox2272位点不兼容。" regulatory 1461..1470 /regulatory_class="other" /note="脊椎动物强启动翻译的共识序列(Kozak,1987)" PuroR 1467..2063 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /note="赋予对嘌呤霉素的抗性" /translation="MVEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" F2A 2094..2159 /codon_start=1 /product="2A peptide from foot-and-mouth disease virus polyprotein" /note="真核核糖体不能在Gly和Pro残基之间插入肽键,从而产生单独的多肽。" Cleavage site after base 2156" /translation="VKQTLNFDLLKLAGDVESNPGP" regulatory 2169..2178 /regulatory_class="other" /note="脊椎动物强启动翻译的共识序列(Kozak,1987)" CDS 2175..2852 /codon_start=1 /product="noncytotoxic tetrameric variant of DsRed fluorescent protein (Strack et al., 2008)" /note="哺乳动物密码子优化" /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH LFQ" loxP511 2863..2896 /bound_moiety="Cre recombinase" /note="Cre介导的重组发生在8-bp核心序列(GTATACAT)中。loxP511位点彼此兼容,但与野生型loxP位点不兼容。" ncRNA complement(2961..3002) /note="小鼠miR-155 microRNA前体下游的序列(Uva等人,2013)" /ncRNA_class="miRNA" ncRNA complement(3066..3092) /note="小鼠miR-155 microRNA前体上游的序列(Uva等人,2013)" /ncRNA_class="miRNA" EmGFP complement(3152..3871) /codon_start=1 /product="Emerald GFP" /note="哺乳动物密码子优化" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" regulatory 3868..3877 /regulatory_class="other" /note="脊椎动物强启动翻译的共识序列(Kozak,1987)" lox2272 complement(3885..3918) /bound_moiety="Cre recombinase" /note="Cre介导的重组发生在8-bp核心序列(GGATACT)中。lox2272位点彼此兼容,但与loxP或loxN位点不兼容。" loxN complement(3885..3918) /bound_moiety="Cre recombinase" /note="Cre介导的重组发生在8-bp核心序列(GTATACCT)中。loxN位点彼此兼容,但与loxP或lox2272位点不兼容。" loxP511 complement(3965..3998) /bound_moiety="Cre recombinase" /note="Cre介导的重组发生在8-bp核心序列(GTATACAT)中。loxP511位点彼此兼容,但与野生型loxP位点不兼容。" WPRE 4044..4632 /note="土拨鼠肝炎病毒转录后调控元件" Factor Xa site complement(4515..4526) /codon_start=1 /product="Factor Xa recognition and cleavage site" Cleavage site after base 4514" /translation="IEGR" 3' LTR 4689..5204 /note="小鼠胚胎干细胞病毒3'长末端重复序列" primer_bind complement(5373..5389) /note="常见的测序引物,多种相似变体之一" lac operator 5397..5413 /bound_moiety="lac repressor encoded by lacI" /note="lac阻遏物与lac操作员结合,抑制大肠杆菌中的转录。这种抑制可以通过添加乳糖或异丙基β-D-硫代吡喃半乳糖苷(IPTG)来缓解。" lac promoter complement(5421..5451) /note="大肠杆菌lac操纵子的启动子" /note="此反向特征有3个分段:1:5421。.5427/#fffff/-10 2:5428。.545/#fffff 3:5446。.5451/#fffff/-35" CAP binding site 5466..5487 /bound_moiety="E. coli catabolite activator protein" /note="CAP结合在cAMP存在下激活转录。" ori complement(5775..6363) /direction=LEFT /note="高拷贝数ColE1/pMB1/pBR322/pUC复制起点" AmpR complement(6534..7394) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="对氨苄青霉素、羧苄青霉素和相关抗生素产生耐药性" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" AmpR promoter complement(7395..7499) /gene="bla"
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