产品声明
- For Research Use Only
All products available through the MiaoLing Plasmid Platform are intended for research use only. They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo. - Plasmid Sequence and Experimental Performance
Plasmid products are provided and sold based on the corresponding reference/template plasmid. MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes. - Sequence Verification of Certain Vector Products
For certain vector products, even the original manufacturer may not have fully verified the complete plasmid sequence. If the sequence of the key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes. - After-Sales Period for Small-Pack Plasmid Products
The after-sales service period for small-pack plasmid products is three months from the date of receipt. Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement. - Research-Related Risks and Limitation of Liability
Scientific research is inherently exploratory and involves a significant degree of uncertainty. To the maximum extent permitted by applicable law, MiaoLing shall not be liable for any additional economic losses, indirect losses, or other liabilities arising from the use of our products or from research outcomes. - Citation in Chinese Publications
For publications in Chinese, please cite the product as follows:
产品名称（产品货号）由淼灵质粒平台提供。 - Citation in English Publications
For publications in English, please cite the product as follows:
“Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”
质粒元件
AmpR promoter 222..326
/gene="bla"
AmpR 327..1187
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="对氨苄青霉素、羧苄青霉素和相关抗生素产生耐药性"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
ori 1358..1946
/direction=RIGHT
/note="高拷贝数ColE1/pMB1/pBR322/pUC复制起点"
CAP binding site 2234..2255
/bound_moiety="E. coli catabolite activator protein"
/note="CAP结合在cAMP存在下激活转录。"
lac promoter 2270..2300
/note="大肠杆菌lac操纵子的启动子"
lac operator 2308..2324
/bound_moiety="lac repressor encoded by lacI"
/note="lac阻遏物与lac操作员结合，抑制大肠杆菌中的转录。这种抑制可以通过添加乳糖或异丙基β-D-硫代吡喃半乳糖苷（IPTG）来缓解。"
primer_bind 2332..2348
/note="常见的测序引物，多种相似变体之一"
SV40 poly(A) signal 2397..2518
/note="SV40聚腺苷酸化信号"
BleoR complement(2648..3022)
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/note="对博莱霉素、静脉霉素和Zeocin（TM）具有抗性"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
EM7 promoter complement(3041..3088)
/note="合成细菌促进剂"
SV40 promoter complement(3136..3465)
/note="SV40增强子和早期启动子"
SV40 ori 3150..3285
/note="SV40复制起点"
f1 ori complement(3479..3907)
/direction=LEFT
/note="f1噬菌体复制起点；箭头表示（+）链合成的方向"
bGH poly(A) signal 3953..4177
/note="牛生长激素多聚腺苷酸化信号"
5' LTR (truncated) 4209..4389
/note="HIV-1截短5'长末端重复序列（LTR）"
primer_bind 4895..4911
/note="常见的测序引物，多种相似变体之一"
WPRE 4914..5502
/note="土拨鼠肝炎病毒转录后调控元件"
Factor Xa site 5020..5031
/codon_start=1
/product="Factor Xa recognition and cleavage site"
Cleavage site after base 5031"
/translation="IEGR"
E2A complement(6909..6968)
/codon_start=1
/product="2A peptide from equine rhinitis A virus
polyprotein"
/note="真核核糖体不能在Gly和Pro残基之间插入肽键，从而产生单独的多肽。"
Cleavage site after base 6911"
/translation="QCTNYALLKLAGDVESNPGP"
T2A complement(8439..8492)
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/note="真核核糖体不能在Gly和Pro残基之间插入肽键，从而产生单独的多肽。"
Cleavage site after base 8441"
/translation="EGRGSLLTCGDVEENPGP"
P2A complement(9471..9527)
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/note="真核核糖体不能在Gly和Pro残基之间插入肽键，从而产生单独的多肽。"
Cleavage site after base 9473"
/translation="ATNFSLLKQAGDVEENPGP"
minimal CMV promoter complement(10701..10739)
/note="人巨细胞病毒（CMV）即刻早期启动子"
tetracycline response element 10772..11042
/bound_moiety="tetracycline repressor TetR"
/note="包含四环素算子tetO的七个拷贝"
cPPT/CTS 11099..11216
/note="HIV-1的中央多泌尿道和中央终止序列"
RRE 11743..11976
/note="HIV-1的Rev反应元件（RRE）允许Rev依赖性mRNA从细胞核输出到细胞质。"
HIV-1 Psi 12469..12594
/note="人类免疫缺陷病毒1型包装信号"
5' LTR (truncated) 12641..12821
/note="HIV-1截短5'长末端重复序列（LTR）"
CMV promoter complement(12839..13037)
/note="人巨细胞病毒（CMV）即刻早期启动子"
CMV enhancer 13039..13418
/note="人类巨细胞病毒即刻早期增强子"
