产品声明
- For Research Use Only
All products available through the MiaoLing Plasmid Platform are intended for research use only. They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo. - Plasmid Sequence and Experimental Performance
Plasmid products are provided and sold based on the corresponding reference/template plasmid. MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes. - Sequence Verification of Certain Vector Products
For certain vector products, even the original manufacturer may not have fully verified the complete plasmid sequence. If the sequence of the key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes. - After-Sales Period for Small-Pack Plasmid Products
The after-sales service period for small-pack plasmid products is three months from the date of receipt. Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement. - Research-Related Risks and Limitation of Liability
Scientific research is inherently exploratory and involves a significant degree of uncertainty. To the maximum extent permitted by applicable law, MiaoLing shall not be liable for any additional economic losses, indirect losses, or other liabilities arising from the use of our products or from research outcomes. - Citation in Chinese Publications
For publications in Chinese, please cite the product as follows:
产品名称（产品货号）由淼灵质粒平台提供。 - Citation in English Publications
For publications in English, please cite the product as follows:
“Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”
质粒元件
HIV-1 Psi 458..583
/note="人类免疫缺陷病毒1型包装信号"
RRE 1076..1309
/note="HIV-1的Rev反应元件（RRE）允许Rev依赖性mRNA从细胞核输出到细胞质。"
cPPT/CTS 1804..1920
/note="HIV-1的中央多泌尿道和中央终止序列"
CMV promoter 2010..2213
/note="人巨细胞病毒（CMV）即刻早期启动子"
regulatory 2284..2293
/regulatory_class="other"
/note="脊椎动物强启动翻译的共识序列（Kozak，1987）"
CDS 2293..2358
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
Cleavage site after base 2358"
/translation="DYKDHDGDYKDHDIDYKDDDDK"
EF-1-alpha core promoter 2422..2633
/note="人延长因子EF-1α的核心启动子"
5' LTR (truncated) 2646..2914
/note="人T细胞白血病病毒1型截短5'长末端重复序列"
PuroR 2941..3540
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/note="赋予对嘌呤霉素的抗性"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
WPRE 3547..4135
/note="土拨鼠肝炎病毒转录后调控元件"
Factor Xa site complement(4018..4029)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
Cleavage site after base 4017"
/translation="IEGR"
3' LTR (Delta-U3) 4209..4442
/note="HIV-1自失活3'长末端重复序列"
SV40 poly(A) signal 4514..4635
/note="SV40聚腺苷酸化信号"
SV40 ori 4654..4789
/note="SV40复制起点"
primer_bind complement(4827..4843)
/note="常见的测序引物，多种相似变体之一"
lac operator 4851..4867
/bound_moiety="lac repressor encoded by lacI"
/note="lac阻遏物与lac操作员结合，抑制大肠杆菌中的转录。这种抑制可以通过添加乳糖或异丙基β-D-硫代吡喃半乳糖苷（IPTG）来缓解。"
lac promoter complement(4875..4905)
/note="大肠杆菌lac操纵子的启动子"
CAP binding site 4920..4941
/bound_moiety="E. coli catabolite activator protein"
/note="CAP结合在cAMP存在下激活转录。"
ori complement(5229..5817)
/direction=LEFT
/note="高拷贝数ColE1/pMB1/pBR322/pUC复制起点"
AmpR complement(5988..6848)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="对氨苄青霉素、羧苄青霉素和相关抗生素产生耐药性"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
AmpR promoter complement(6849..6953)
/gene="bla"
primer_bind 7427..7443
/note="常见的测序引物，多种相似变体之一"
