产品声明
- For Research Use Only
All products available through the MiaoLing Plasmid Platform are intended for research use only. They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo. - Plasmid Sequence and Experimental Performance
Plasmid products are provided and sold based on the corresponding reference/template plasmid. MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes. - Sequence Verification of Certain Vector Products
For certain vector products, even the original manufacturer may not have fully verified the complete plasmid sequence. If the sequence of the key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes. - After-Sales Period for Small-Pack Plasmid Products
The after-sales service period for small-pack plasmid products is three months from the date of receipt. Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement. - Research-Related Risks and Limitation of Liability
Scientific research is inherently exploratory and involves a significant degree of uncertainty. To the maximum extent permitted by applicable law, MiaoLing shall not be liable for any additional economic losses, indirect losses, or other liabilities arising from the use of our products or from research outcomes. - Citation in Chinese Publications
For publications in Chinese, please cite the product as follows:
产品名称（产品货号）由淼灵质粒平台提供。 - Citation in English Publications
For publications in English, please cite the product as follows:
“Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”
质粒元件
LTR 28..767
RDV114 1222..2814
/codon_start=1
/translation="MKLPTGMVILCSLIIVRAGFDDPRKAIALVQKQHGKPCECSGGQV
SEAPPNSIQQVTCPGKTAYLMTNQKWKCRVTPKNLTPSGGELQNCPCNTFQDSMHSSCY
TEYRQCRANNKTYYTATLLKIRSGSLNEVQILQNPNQLLQSPCRGSINQPVCWSATAPI
HISDGGGPLDTKRVWTVQKRLEQIHKAMHPELQYHPLALPKVRDDLSLDARTFDILNTT
FRLLQMSNFSLAQDCWLCLKLGTPTPLAIPTPSLTYSLADSLANASCQIIPPLLVQPMQ
FSNSSCLSSPFINDTEQIDLGAVTFTNCTSVANVSSPLCALNGSVFLCGNNMAYTYLPQ
NWTGLCVQASLLPDIDIIPGDEPVPIPAIDHYIHRPKRAVQFIPLLAGLGITAAFTTGA
TGLGVSVTQYTKLSHQLISDVQVLSGTIQDLQDQVDSLAEVVLQNRRGLDLLTAEQGGI
CLALQEKCCFYANKSGIVRNKIRTLQEELQKRRESLASNPLWTGLQGFLPYLLPLLGPL
LTLLLILTIGPCVF"
4070A 2815..2916
/codon_start=1
/translation="NRLVQFVKDRISVVQALVLTQQYHQLKPIEYEP"
SV40 poly(A) signal 3046..3167
/note="SV40聚腺苷酸化信号"
f1 ori complement(3174..3629)
/direction=LEFT
/note="f1噬菌体复制起点；箭头表示（+）链合成的方向"
AmpR promoter 3656..3760
/gene="bla"
SV40 promoter 3762..4119
/note="SV40增强子和早期启动子"
SV40 ori 3970..4105
/note="SV40复制起点"
NeoR/KanR 4154..4948
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="赋予对新霉素、卡那霉素和G418（遗传霉素（R））的抗性"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
HSV TK poly(A) signal 5180..5227
/note="单纯疱疹病毒胸苷激酶多聚腺苷酸化信号（Cole和Stacy，1985）"
ori 5556..6144
/direction=RIGHT
/note="高拷贝数ColE1/pMB1/pBR322/pUC复制起点"
