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pHR_Gal4UAS_humanTRAIL _IRES_mCherry_PGK_TBfp

Catalog Number P1654
Alternative Names 等效质粒Plasmid#85432
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Product Disclaimer

  1. For Research Use Only
    All products available through the MiaoLing Plasmid Platform are intended for research use only. They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.
  2. Plasmid Sequence and Experimental Performance
    Plasmid products are provided and sold based on the corresponding reference/template plasmid. MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.
  3. Sequence Verification of Certain Vector Products
    For certain vector products, even the original manufacturer may not have fully verified the complete plasmid sequence. If the sequence of the key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.
  4. After-Sales Period for Small-Pack Plasmid Products
    The after-sales service period for small-pack plasmid products is three months from the date of receipt. Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.
  5. Research-Related Risks and Limitation of Liability
    Scientific research is inherently exploratory and involves a significant degree of uncertainty. To the maximum extent permitted by applicable law, MiaoLing shall not be liable for any additional economic losses, indirect losses, or other liabilities arising from the use of our products or from research outcomes.
  6. Citation in Chinese Publications
    For publications in Chinese, please cite the product as follows:
    Product names and IDs are provided by the MiaoLing Plasmid Platform.
  7. Citation in English Publications
    For publications in English, please cite the product as follows:
    “Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”

Related Publications

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Product Documents

Plasmid Elements

AmpR promoter 1126..1230 /gene="bla" AmpR 1231..2091 /codon_start=1 /gene="bla" /product="beta-lactamase" /note="对氨苄青霉素、羧苄青霉素和相关抗生素产生耐药性" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" ori 2262..2850 /direction=RIGHT /note="高拷贝数ColE1/pMB1/pBR322/pUC复制起点" SV40 promoter 3096..3425 /note="SV40增强子和早期启动子" SV40 ori 3276..3411 /note="SV40复制起点" intron 4559..4624 /note="SV40(猿猴病毒40)小t抗原内含子" SV40 NLS 4754..4774 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /translation="PKKKRKV" SV40 poly(A) signal 5199..5333 /note="SV40聚腺苷酸化信号" 3' LTR 5502..6135 /note="HIV-1的3'长末端重复序列(LTR)" HIV-1 Psi 6182..6307 /note="人类免疫缺陷病毒1型包装信号" RRE 6799..7032 /note="HIV-1的Rev反应元件(RRE)允许Rev依赖性mRNA从细胞核输出到细胞质。" cPPT/CTS 7544..7661 /note="HIV-1的中央多泌尿道和中央终止序列" human FL TRAIL 7992..8837 /codon_start=1 /translation="MAMMEVQGGPSLGQTCVLIVIFTVLLQSLCVAVTYVYFTNELKQM QDKYSKSGIACFLKEDDSYWDPNDEESMNSPCWQVKWQLRQLVRKMILRTSEETISTVQ EKQQNISPLVRERGPQRVAAHITGTRGRSNTLSSPNSKNEKALGRKINSWESSRSGHSF LSNLHLRNGELVIHEKGFYYIYSQTYFRFQEEIKENTKNDKQMVQYIYKYTSYPDPILL MKSARNSCWSKDAEYGLYSIYQGGIFELKENDRIFVSVTNEHLIDMDHEASFFGAFLVG " IRES 8856..9429 /note="脑心肌炎病毒(EMCV)的内部核糖体进入位点(IRES)" mCherry 9448..10158 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /note="哺乳动物密码子优化" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" PGK promoter 10243..10742 /note="小鼠磷酸甘油酸激酶1启动子" TagBFP 10763..11464 /codon_start=1 /product="monomeric blue fluorescent protein" /note="哺乳动物密码子优化" /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC DLPSKLGHKLN" WPRE 11500..12088 /note="土拨鼠肝炎病毒转录后调控元件"
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