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pHR_PGK_antiHer24D5-7_synNotch_Gal4VP64

产品货号 P1652
产品别称 等效质粒Plasmid#85424
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价格: ¥480.00
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基本信息

产品简介

产品声明

  1. For Research Use Only
    All products available through the MiaoLing Plasmid Platform are intended for research use only. They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.
  2. Plasmid Sequence and Experimental Performance
    Plasmid products are provided and sold based on the corresponding reference/template plasmid. MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.
  3. Sequence Verification of Certain Vector Products
    For certain vector products, even the original manufacturer may not have fully verified the complete plasmid sequence. If the sequence of the key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.
  4. After-Sales Period for Small-Pack Plasmid Products
    The after-sales service period for small-pack plasmid products is three months from the date of receipt. Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.
  5. Research-Related Risks and Limitation of Liability
    Scientific research is inherently exploratory and involves a significant degree of uncertainty. To the maximum extent permitted by applicable law, MiaoLing shall not be liable for any additional economic losses, indirect losses, or other liabilities arising from the use of our products or from research outcomes.
  6. Citation in Chinese Publications
    For publications in Chinese, please cite the product as follows:
    产品名称(产品货号)由淼灵质粒平台提供。
  7. Citation in English Publications
    For publications in English, please cite the product as follows:
    “Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”

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产品文件

质粒元件

signal peptide 1..63 Myc 64..93 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /translation="EQKLISEEDL" Her2scFv_humAb4D5-7 94..831 NL domains through cytoplasmic 832..1809 Gal4VP64 1810..2445 /codon_start=1 /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSAAAGGSGGSGGSDALD DFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGS" 3' LTR (Delta-U3) 3206..3439 /note="HIV-1自失活3'长末端重复序列" AmpR promoter 4270..4374 /gene="bla" AmpR 4375..5235 /codon_start=1 /gene="bla" /product="beta-lactamase" /note="对氨苄青霉素、羧苄青霉素和相关抗生素产生耐药性" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" ori 5406..5994 /direction=RIGHT /note="高拷贝数ColE1/pMB1/pBR322/pUC复制起点" SV40 promoter 6240..6569 /note="SV40增强子和早期启动子" SV40 ori 6420..6555 /note="SV40复制起点" intron 7703..7768 /note="SV40(猿猴病毒40)小t抗原内含子" SV40 NLS 7898..7918 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /translation="PKKKRKV" SV40 poly(A) signal 8343..8477 /note="SV40聚腺苷酸化信号" 3' LTR 8645..9278 /note="HIV-1的3'长末端重复序列(LTR)" HIV-1 Psi 9325..9450 /note="人类免疫缺陷病毒1型包装信号" RRE 9947..10180 /note="HIV-1的Rev反应元件(RRE)允许Rev依赖性mRNA从细胞核输出到细胞质。" cPPT/CTS 10694..10811 /note="HIV-1的中央多泌尿道和中央终止序列" PGK promoter 10871..11370 /note="小鼠磷酸甘油酸激酶1启动子"
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