产品声明
- For Research Use Only
All products available through the MiaoLing Plasmid Platform are intended for research use only. They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo. - Plasmid Sequence and Experimental Performance
Plasmid products are provided and sold based on the corresponding reference/template plasmid. MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes. - Sequence Verification of Certain Vector Products
For certain vector products, even the original manufacturer may not have fully verified the complete plasmid sequence. If the sequence of the key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes. - After-Sales Period for Small-Pack Plasmid Products
The after-sales service period for small-pack plasmid products is three months from the date of receipt. Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement. - Research-Related Risks and Limitation of Liability
Scientific research is inherently exploratory and involves a significant degree of uncertainty. To the maximum extent permitted by applicable law, MiaoLing shall not be liable for any additional economic losses, indirect losses, or other liabilities arising from the use of our products or from research outcomes. - Citation in Chinese Publications
For publications in Chinese, please cite the product as follows:
产品名称（产品货号）由淼灵质粒平台提供。 - Citation in English Publications
For publications in English, please cite the product as follows:
“Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”
质粒元件
HIV-1 Psi 488..613
/note="人类免疫缺陷病毒1型包装信号"
5' LTR (truncated) 660..840
/note="HIV-1截短5'长末端重复序列（LTR）"
CMV promoter complement(858..1056)
/note="人巨细胞病毒（CMV）即刻早期启动子"
CMV enhancer 1058..1437
/note="人类巨细胞病毒即刻早期增强子"
AmpR promoter 1703..1807
/gene="bla"
AmpR 1808..2668
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="对氨苄青霉素、羧苄青霉素和相关抗生素产生耐药性"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
ori 2839..3427
/direction=RIGHT
/note="高拷贝数ColE1/pMB1/pBR322/pUC复制起点"
SV40 poly(A) signal 3878..3999
/note="SV40聚腺苷酸化信号"
Zeo complement(4129..4503)
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/note="对博莱霉素、静脉霉素和Zeocin（TM）具有抗性"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
EM7 promoter complement(4522..4569)
/note="合成细菌促进剂"
SV40 promoter complement(4617..4946)
/note="SV40增强子和早期启动子"
f1 ori complement(4960..5388)
/direction=LEFT
/note="f1噬菌体复制起点；箭头表示（+）链合成的方向"
bGH poly(A) signal 5434..5658
/note="牛生长激素多聚腺苷酸化信号"
5' LTR (truncated) 5690..5870
/note="HIV-1截短5'长末端重复序列（LTR）"
EF-1-alpha promoter 6475..7653
/note="人伸长因子EF-1α的强组成型启动子"
regulatory 7660..7669
/regulatory_class="other"
/note="脊椎动物强启动翻译的共识序列（Kozak，1987）"
MS2-N55K 7669..8055
/codon_start=1
/product="bacteriophage MS2 coat protein"
/note="以高亲和力结合病毒RNA中的特定茎环结构（Lim等人，1994）"
/translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
VKAMQGLLKDGNPIPSAIAANSGIY"
SV40 NLS 8110..8130
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/translation="PKKKRKV"
p65 8146..8688
/codon_start=1
/product="C-terminal portion of the p65 subunit of mouse
NF-kappa-B"
/translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
FSSIADMDFSALLSQISS"
CDS 8713..9084
/codon_start=1
/product="C-terminal activation domain from the human heat
shock transcription factor HSF1"
/translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
PTISLLTGSEPPKAKDPTVS"
T2A 9100..9153
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/note="真核核糖体不能在Gly和Pro残基之间插入肽键，从而产生单独的多肽。"
Cleavage site after base 9150"
/translation="EGRGSLLTCGDVEENPGP"
HygR 9157..10176
/codon_start=1
/product="hygromycin B phosphotransferase"
/note="赋予对潮霉素的抗性"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
"
WPRE 10216..10804
/note="土拨鼠肝炎病毒转录后调控元件"
cPPT/CTS 10857..10974
/note="HIV-1的中央多泌尿道和中央终止序列"
RRE 11501..11734
/note="HIV-1的Rev反应元件（RRE）允许Rev依赖性mRNA从细胞核输出到细胞质。"
