产品声明
- For Research Use Only
All products available through the MiaoLing Plasmid Platform are intended for research use only. They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo. - Plasmid Sequence and Experimental Performance
Plasmid products are provided and sold based on the corresponding reference/template plasmid. MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes. - Sequence Verification of Certain Vector Products
For certain vector products, even the original manufacturer may not have fully verified the complete plasmid sequence. If the sequence of the key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes. - After-Sales Period for Small-Pack Plasmid Products
The after-sales service period for small-pack plasmid products is three months from the date of receipt. Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement. - Research-Related Risks and Limitation of Liability
Scientific research is inherently exploratory and involves a significant degree of uncertainty. To the maximum extent permitted by applicable law, MiaoLing shall not be liable for any additional economic losses, indirect losses, or other liabilities arising from the use of our products or from research outcomes. - Citation in Chinese Publications
For publications in Chinese, please cite the product as follows:
产品名称（产品货号）由淼灵质粒平台提供。 - Citation in English Publications
For publications in English, please cite the product as follows:
“Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”
质粒元件
2u ori 69..1411
/note="酵母2u质粒复制起点"
FRT 401..448
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP介导的重组发生在8-bp核心序列TCTAGAAA中（Turan和Bode，2011）。"
AmpR promoter 1438..1542
/gene="bla"
AmpR 1543..2403
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="对氨苄青霉素、羧苄青霉素和相关抗生素产生耐药性"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
ori 2574..3162
/direction=RIGHT
/note="高拷贝数ColE1/pMB1/pBR322/pUC复制起点"
terminator 3643..3807
/gene="S. cerevisiae ADH1"
/note="酿酒酵母乙醇脱氢酶1（ADH1）基因的转录终止子"
MCS1 3915..3982
TEF1 promoter complement(3998..4396)
/gene="S. cerevisiae TEF1"
/note="EF-1α启动子"
primer_bind complement(4321..4339)
GAP promoter 4452..5095
/gene="S. cerevisiae TDH3"
/note="甘油醛-3-磷酸脱氢酶启动子"
MCS2 5107..5169
terminator 5170..5417
/gene="S. cerevisiae CYC1"
/note="CYC1的转录终止子"
f1 ori 5625..6080
/direction=RIGHT
/note="f1噬菌体复制起点；箭头表示（+）链合成的方向"
CDS complement(6211..7014)
/codon_start=1
/gene="S. cerevisiae URA3"
/product="orotidine-5'-phosphate decarboxylase, required
for uracil biosynthesis"
/note="酵母营养缺陷型标记物，可与5-氟乳清酸（5-FOA）进行复选"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
URA3 promoter complement(7015..7230)
/gene="S. cerevisiae URA3"
