产品声明
For Research Use Only research use only . They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.Plasmid Sequence and Experimental Performance reference/template plasmid . MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.Sequence Verification of Certain Vector Products key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.After-Sales Period for Small-Pack Plasmid Products three months from the date of receipt . Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.Research-Related Risks and Limitation of Liability Citation in Chinese Publications 产品名称（产品货号）由淼灵质粒平台提供。 Citation in English Publications “Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”
质粒序列
一键复制序列（有部分质粒是非全长序列）
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCCTCCAAAAAAGCCTCCTCACTACTTCTGGAATAGCTCAGAGGCCGAGGCGGCCTCGGCCTCTGCATAAATAAAAAAAATTAGTCAGCCATGGGGCGGAGAATGGGCGGAACTGGGCGGAGTTAGGGGCGGGATGGGCGGAGTAAGACCCGCACCCGTTCCCAAACTGCTTATTAAAGGGGGTATGGAGGTGCTGGACCTTGTGACAGGGCCAGACAGTGTGACAGAAATAGAAGCAGTCCTGTTTTGACAAGTTGCCTCTGGAAGCCTCTACAATGCCTCTCTTCTTTTTCTCCAGAGTAAGCGGAGGCCAGGGGCCCCCGGCCTCTGCTTAATACTAAAAAAAACAGCTGTTGTCATAGTAATGATTGGGTGGAAACATTCTAGGCCTGGGTGGAGAGGCTTTTTGCTTCCTCTTGCAAAACCACACTGCCCTCTGGAGGGCAGTTGCCTAGCAACTAATTAAAAGAGGATGTCGCACGGCCAGCTGCGGTCAGTTAGTCACTTCCTGCTTAACTGACTTGACATTTTCTATTTTAAGAGTCGGGAGGAAAATTACTGTGTTGGAGGCCCTCCGCCATCTTCTGAAGCTGATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTATGGCTGATTATGATCTCTAGTCAAGGCACTATACATCAAATATTTTTCCATAATTTTCTTGTATAGCAGTGCAGCTTTTTCCTTTGTGGTGTAAATAGCAAAGCAAGCAAGAGTTCTATTACTAAACACAGCATGACTCAAAAAACTTAGCAATTCTGAAGGAAAGTCCTTGGGGTCTTCTACCTTTCTCTTCTTTTTTGGAGGAGTAGAATGTTGAGAGTCAGCAGTAGCCTCATCATCACTAGATGGCATTTCTTCTGAGCAAAACAGGTTTTCCTCATTAAAGGCATTCCACCACTGCTCCCATTCATCAGTTCCATAGGTTGGAATCTAAAATACACAAACAATTAGAATCAGTAGTTTAACACATTATACACTTAAAAATTTTATATTTACCTTAGAGCTTTAAATCTCTGTAGGTAGTTTGTCCAATTATGTCACACCACAGAAGTAAGGTTCCTTCACAAAGATCCTCTAGCTACCTAGCTAGATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCCAGCTTACCTCCCGGTGGTGGGTCGGTGGTCCCTGGGCAGGGGTCTCCCGATCCCGGACGAGCCCCCAAATGAAAGACCCCCGCTGACGGGTAGTCAATCACTCAGAGGAGACCCTCCCAAGGAACAGCGAGACCACAAGTCGGATGCAACTGCAAGAGGGTTTATTGGATACACGGGTACCCGGGCGACTCAGTCAATCGGAGGACTGGCGCCCCGAGTGAGGGGTTGTGGGCTCTTTTATTGAGCTCGGGGAGCAGAAGCGCGCGAACAGAAGCGAGAAGCGAACTGATTGGTTAGTTCAAATAAGGCACAGGGTCATTTCAGGTCCTTGGGGCACCCTGGAAACATCTGATGGTTCTCTAGCTTGCCAAACCTACAGGTGGGGTCTTTCATTCCCCCCTTTTTCTGGAGACTAAATAAAATCTTTTATTTTATCTATGGCTCGTACTCTATAGGCTTCAGCTGGTGATATTGTTGAGTCAAAACTAGAGCCTGGACCACTGATATCCTGTCTTTAACAAATTGGACTAATCGATTGAGAATGCAAGGTCCAAAGAGTAAGATCAGTAAGAGTACTATTAGAGGTCCCATGATGGTGGAGATTAAGGTGGTAAACCAGGGGGATCTATTAAACAGCCCTTCGAACCATCCTTGGCCTGTCTCAAATAGTTTTTGTCTCTGATTAAGCCTTTCTCTTAATTTGGCCATGCTGTCTCTCACTAGCCCCGTGTGGTCTGCATAAAAACAACATTCTTCTTTTAGGGCTGCGCAGAGACCTCCCTCCTTTAGGAATAGCAAATCTAGGCCTCTGCGGTTCTGTAGGACTACTTCAGACAACGAGGTCAGTGACTTTTCTAGGTTGGTAATTGACTTTTCGACTTCGTTGAGGTCTGTCTGGATAGCGGCATGAAGCTGCTCAAACTGCTGGGTTTTAATTAAGGCAGTGGTCCCCGTCCCTATTCCAGCTGCAATCCCTCCCATGGTTAATCCTCCTAGTAGAAGGGCCAGGGTCAATGATACTGGCTCTCTTTTATATTTGGTACGCTGTTCAAGCTGACCATACATATAATCGGGGGAGTGGTAAATTACTCTGGGCCAGAGTTCAACTAATACACAATAATCTGTGGTTAGATTGAGCACCGTGGTGGACAAGCAGGGAGTCAATCCAGTGCTGCAAGCCCACATTGTTCCGGCGGGTGCTGCAAGGTAGTAGGATCCTGAGCCGGCGCTTTGGGTGGTGTTACATAAGGCCTGGTGAGTTTTAGGTACTGCCCCCATGCATAGGCCCTGTCCTGTCACTTCAGATAGGGTAAGCTTATGTTGGGAAGTGGCCGTACAGTTGGCCGGAGCGGTGGAATGATTGGTATAAGTGCCCACGACCGCTACTCCTTCGTAATAAGGAGGTCCCGACACTAAGCACAGCCAACATTCTTGGGTCTTGTCGGGATTGGTGAGGTTAAGCGCCTGATAGGCTCCTTTGACTAGAGCTAGTAGTCTATCTCCAGTTCCTGGGGGTGGCTGTGGGACACTTGGACTTGTAGGGGAGGTTGAGGGTGTACTGGTAGTGGAAGGGGGGTAACTGGTATTGAGGGGGCTAGGTGGCTGTGGAGCCGGTACAATCTCTATTGGTGAGGAAGGGAGTCTTTGGTCGGGTAATACTGGGTTGGGCCCTATGGGGACTCGGGGTCCCACATTAAGGACCTGCCGGGTCAGGGAGAACATGGTAATAGGATCTGTTCCTGTCCGGTACAGTCTCAGTCCCCACGATTTGGGCCCGTCCCAGTTAGCCTTTTTTCCTGCATCAGTGAATTCTAGGACTAGAGGGTTGCATCTGCCCCCTCGAGTAGCCCCTTGGAAGGAATTGGATACTTTGGAGAGGTCGTAGCAGGGGCCACAGGCAACTTTAGAGCATCCCGTGTCCCAGGGGGTGTTACCGCGCTTAAGGGAGATTAGGTCCCACGATGATGTGGGCTTCCAGTAAGCCTGTCCGGTGGTTTCACACCCCCATTTACCACAGTAGCCCTCTCCTGGTCCCCCACACCCCGACTTTACGGTATGCCCAGGGCACACGTAAAAGTCAAAAGTCCGGGTCCGCTGTCTCCCTGCGGGGTACTTGCAGCCATACCCGACATACGGTTCCTGGTCTGAAGGGTCCCACTCCTCTCCGACCAGATCACATAGATCAAAATATAATTTTGGGAAGGCATCTTGTACAGTTCCCAGGAGGGAGGTGGCATTGGCGGTACGCCCAGTCATCAGGTTGGTGACTCTCCAGGTTACATTAAAGACCTGATGGGGGCTCTCTGCCATCCCTACTCCTAACAGGACTCCCATGACTATTAAGGGCTTCCACGGGTTAATCTTATCTTGAGGGGGTTTTGAGAGCGTTGAACGCGCCATGTCCGTCCAGAGGATGGTCCACTCTCGGTGTCGGCCGCCTTTACGTGGGCTGCGTGTATCCAAGCTGCGATACCGTCTACTTTGAGGGCGGTGGGGGTGGTCAGCAGGACTGTGTAAGGTCCTTTCCAGCGAGGTTCTAGGTTCTTGGTTTGATGTCGGCGGACCCACACTGTGTCGCCGACCCGGTAAGGGTGAGGCACCACCGGCCGGTCCAGTTGTTCTTGGTAAGCTGCCGCCAGTGGTCTCCAAACTTCGTGCTGGACTAAGTAGAGAGCCTGTAAGTGACCAGTGGTCTCCAAACTTCGTGCTGGACTAAGTAGAGAGCCTGTAAGTGAGCTTGGAGAGAGGGGCTGTTAGTAACTCTGGTCATGTCAGGGTCAGGGAAGTTTACAAGGGGCGGGGGTGCCCCATATAAGATCTCATATGGGGTGAGGCCATGGGGGCCCGGCGTGTTGCGGGCTCGGTACAGGGCTAAGGGGAGTAGGAGCACCCAGTCTCTAGAGCCAGTTGCAAGCGTTAATTTAGTTAAAGTCTCCTTGATGGTCCTATTCATTCTTTCTACCTGACCTGAGCTTTGGGGTCTGTATGCACAATGTAATTTCCAATCAATCCCCAACAGATCGGCCACTGTCTGACTCACCTTGGAGACGAAGGCAGGCCCATTGTCAGTTCCCAATACCTGCGGCATGCCGAACCTAGGGAAGATCTCTTCTAGCAGTTTCTTGGTCACGACCTTGGCGGTTTCTTTCTTAGTTGGGAAAGCTTCTATCCAGCCAGAAAAAGTATCTACAAAAACTAAAAGATACTTATAGCCATACAATCCAGGTTTTACCTCGGTGAAATCGATCTCCCAGTGTGTGCCAGGCCGATGCCCGCGGACCCTAGTTCCTTGCTTAACGGCAGACTTGCTGGCATTGACTTGTGCACAAGCTTTGCAGGTCTCAGTGATATTTTTGAGTGTTCGATCCCGGTTCAGCATGTAGTAGGGACTGGGGCTTCTCTCTAGGAGAGCCTTTGTTTTTGAGAAGCTGAGGTGGGTCAATTGGTGAAGAAAGTCTAGTAACTCAAAGGTGAATTGATCAGGCATAACAGGCTTTCCTTGATAGACCCAATATTTCTTCGCACTGTCATAAGTGGCTCCTAGTTTGGTCAAATCCTTTGTGTCAGTTACTGTATAGTGAAAGTGTTCATGGGTATAGGGGGTTGAGTTTTCTATCAGAAGTGTGGAAGTTCCTGGAGTTTCTCTAGTGGCTACTTCTCGGGCCGCTTGGTCGGCCATCCGGTTGCCCCTGGCCTCTGCGCTGTTTCCTTTTTGATGTCCCGGGCAATGAATTATGCTAAGTCTTTTGGGCAAGAAGAGAGCCTTTAGTAGGGCTAAGATCTCGTCCTTGTTCTTGATCTCTTTTCCTTCTGATGTGAGCAACCCGCGCCTTCTGTATATTTCTCCATGAATATGGGCGGTGGCAAAAGCGTAACGGCTATCAGTATAAACATTTAGCTTCTTACCTTCTGCCATCTTTAGGGCTTGGGTGAGCGCTATCAGTTCAGCTCTTTGGGCCGATGTCCCGGCTGGCAATGCCCTGGCCCAGATTACCTCAGTCTCAGTGGTCACCGCTGCTCCGGCCTTACGCTGCCCTTCTTGCAGGAAGCTGCTCCCATCCGTGTACCAGGTGTGGTCGGCGTCTGGGAGGGGCTGGTCCGTAAGATCTGATCTAGTTCCGTGGGCTTCAGCCAAGATGTCAAGGCAGTCATGTTGCAGCCCCTCCTCAGGCAGAGGGAGCAGCGTAGCTGGATTTAGGGCCACTACTGGCCCGAACTGGACCCGGTCCGTGTCCAGGAGCAGGGCTTGGTAATGGGTCATCCGGGCATTGGAGAGCCAGCGATCAGGGGGTTGCTTAACTAGTGCCTCTACGGCATGGGGGGCCAGAATGACCAACGGCTGTCCCATAGTGAGCTTGCCAGCATCTTTTGTCAGAACTGCAATGGCTGCCACCATCCGTAGGCAGGGGGGCCAGCCAGCTGCCACTGGGTCTAGCTTTTTAGACAGGTAGGCCACCGGCCGACGCCAAGGTCCCAGCTTTTGCGTTAGGACGCCTTTGGCGTAGCCCTGCTTCTCGTCGACAAAGAGTTCAAAGGGCTTAGTCAAATCTGGCAACCCCAGGGCTGGGGCAGTTAGAAGAGCTTGCTTGATTTCTTGATAGGCCTTTTGTTGGTCTGGGCCCCAATTAAACAGAGTCCCCGTTTTGGTGAGAGGGTACAAGGGGGCTGCCATTTCTGCAAACCCAGGGATCCAGAGGCGACAGAAGCCTGCCGTCCCTAGGAACTCCCTTAGTTGTCGAGGGGTCTTCGGAGTAGGCTGCCCCATCACAGTCTCTTTTCTGGCCTCAGTCAGCCATCTCTGACCCTCTTTTAGAAGATACCCCAGATACTTGACCTGTTTCTGGCAAATTTGGGCTTTCTTGGCCGAGGCCCGATACCCGAGGTTCCCTAGGGTTTGTAACAGGGCCCGAGTACCTTGTTGGCAGTCTAGCTCAGAAGTGGCGGCCAGCAGTAAGTCATCCACGTACTGTAGCAGGATCAAGTCTGGGTGCTGGATCCGGAAGTCTGCTAGGTCTCTGTGCAGTGCCTCATCAAACAGGGTGGGACTGTTTTTGAAACCCTGTGGGAGTCTGGTCCAGGTCAATTGTCCTGAGATTCCCATCTCTGGATCTCTCCACTCAAAGGCGAAGAGAGGCTGACTGGTGGGGTGGAGTCTCAGGCAGAAAAAGGCATCCTTTAAATCAAGCACAGTGTACCACTGGTGGGACGGTGGGAGCCCGCTCAAGAGGTTGTAAGGGTTGGGCACGGTGGGGTGGATGTCTTCCACCCGCTTGTTGACTTCTCTCAGATCCTGGACAGGCCTATAATCATTAGTCCCTGGTTTCTTAACGGGTAGCAGGGGCGTGTTCCAGGGGGACTGGCAGGGTACCAGTATTCCCTGGTCCAACAGTCTCTGTATGTGGGGCTTGATCCCCAGTCTGGCTTCTTGTGACATGGGGTATTGTTTTATGGACACGGGGGTAGAGGTTGCTTTCAGAGGTATGATCAGAGGAGCTTGGCGAACTGCCAGTCCCATGCCCCCGGTTTCCGCCCAGGCCTGAGGAAAATCAGACAGCCATGTGGACCCTAGAGAAACATCTGGCTCTTTTGAGGTCTCATGTAGCCGATACTCATCTTCTATATTTAGGGTCAACACTTGCAGGGGCTGCCCCATTGGTCCCATAACCTGAGCTCCTGATCCCTCAAAGTGGATTTGGGCTTTTAGTTTAGTCAGCAAATCTCTTCCTAACAGAGGATAGGGACAGTCTGGTACATGGAGGAAAGAGTGGGTGACCTTACCGGTAGCTAGATGTACTTTGCGATCCGTGGTCCAGCGATACCGCTTTCCTCCAGTAGCCCCTTGGACCCAGGCAGACTTATCACTTAGGGGTCCAGGATTTTGGGTCAGCACGGAGTGTTGGGCCCCAGTATCTACCAGGAAGGTGACGGGTTGCCCCCCGACTTTGAGGGTTATCCTGGGTTCAGGGGGGGGCTCCTGACCCTGACCTCCCTAGTCATCTAGGGTCAGGAGGGAGGTCTGGGGTCTTGGTCCCCGAGGTCCTCGTGGTTTCTTGGGACAATCTTTAGCCCAGTGCCCCTTTTCTTTGCAGTAGGCACACTGGTCGCGATCGAGTTGGGACCTCCTTCGTTCTCCTCCCTGTCTATCCTGTTTCTGTCCACTAACGACAGTGGCCAATAGCTTGCTCATCTCTCTATGTCTCCTACGATCTCTTTCTTTCTCTTTCTGCTCATCCTCTGTCCTACGGCGTTCTTCTTTTTCCTCTGTTTCTCTCCTGATACGTTCCTCTCTTTCTTCCGGGGTTTCTCGTTTATTAAAGATCTTTTCTGCCTCTCTAACCAAATCTCCAAGCGTCTTGTTTTTTAAATCTTCTAACCTCTCTAACTTTCTCCCAATGTCTGGGGCAGACTGCCAAATGAAAGACATAGACACATTAGTTTCTTGCCCTGGGTCCTCAGGGTCATAAGGAGTGTACCTGCGATAGGCTTCCTTAAGTCTCTCTAGGAAGGCCGAGGGAGACTCATTGGGCCCTTGTGTTATTCCTTTTACCTTGGCCAAATTGGTGGGGCTTCTGCCCGCGTTTTGGAGACCCGCTAGGAGCAACTGGCGATAGTGGACTAGGTGGTTCCTACCTGCCTGGGTGGTGTAATCCCAGTCTGGGCGCTCGAGGGGAAAAGCGGCATCGACTTCATTGGGCAGTTGAGTGGGGCGCCCATCATCGCCCCGCACCGCCTTTCTAGCCTCTAAGAGCACCCGTTGTTTTTCTTCTCCGGTCAGCAGAGTCCCCAACAGCTGCTGACAGTCGTCCCAGGTGGGCTGATGGGTGATGAGGACAGACTCGATCAGAGCTGTCAGTTTACCTGGATCTTCAGAAAAAGAAGGGTTATTATTTTTCCAGTTGTAAAGGTCAGAAGAGGAGAACGGCCAGTATTGAAGCTGTCCGTTTCCTCCTGCGCGGAGGGGGAATGCCTGCGAGGTAGTGGAGTCGGCCACAGGGGGCTCCCGTCTCCCACGTAGGCGAGATGCCATTGGGGAGGGGTCCGGTGCCTCTCCCGCAGGGGTCGCTTCTCCACCATTTCCGTCCCTGTCGGAAGGGGGTGGTCTTGGGTCCCTATAAGGCGGGGGGTCTTCTGTAAGTAGGTCGATGAGCGGCCCCCCACTGTCAGAAAGAACTTGAGGTTTAGGTTTGGCGCCTAGAGAAGGAGTGAGGGCTGGATAAAGGGAGGATCGAGGCGGGGTCGAACGAGGAGGTTCAAGGGGGAGAGACGGGGCGGATGGAGGAAGAGGAGGCGGAGGCTTAGGGTGTACAAAGGGCTTGACCCAGGGAGGGGGGTCAAAAGCCAAGGCTTCCCAGGTCACGATGTAGGGGACCTGGTCTGGGTGTCCATGCGGGCCAGGTGAAAAGACCTTGATCTTAACCTGGGTGATGAGGTCTCGGTTAAAGGTGCCGTCTCGCGGCCATCCGACGTTAAAGGTTGGCCATTCTGCAGAGCAGAAGGTAACCCAACGTCTCTTCTTGACATCTACCGACTGGTTGTGAGCGATCCGCTCGACATCTTTCCAGTGACCTAAGGTCAAACTTAAGGGAGTGGTAACAGTCTGGCCCATATTTTCAGACAAATACAGAAACACAGTCAGACAGAGACAACACAGAACGATGCCCAAGCTTGGCCAGCTTACCTCCCGGGACAGAGACAACACAGAACGATGCCCAAGCTTGGCCAGCTTACCTCCCGGTGGTGGGTCGGTGGTCCCTGGGCAGGGGTCTCCCGATCCCGGACGAGCCCCCAAATGAAAGACCCCCGCTGACGGGTAGTCAATCACTCAGAGGAGACCCTCCCAAGGAACAGCGAGACCACAAGTCGGATGCAACTGCAAGAGGGTTTATTGGATACACGGGTACCCGGGCGACTCAGTCAATCGGAGGACTGGCGCGCCGAGTGAGGGGTTGTGGGCTCTTTTATTGAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCATTTGCGTCAATGGGGCGGAGTTGTTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGTGAGTCAAACCGCTATCCACGCCCATTGATGTACTGCCAAAACCGCATCACCATGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCCCATAAGGTCATGTACTGGGCATAATGCCAGGCGGGCCATTTACCGTCATTGACGTCAATAGGGGGCGTACTTGGCATATGATACACTTGATGTACTGCCAAGTGGGCAGTTTACCGTAAATACTCCACCCATTGACGTCAATGGAAAGTCCCTATTGGCGTTACTATGGGAACATACGTCATTATTGACGTCAATGGGCGGGGGTCGTTGGGCGGTCAGCCAGGCGGGCCATTTACCGTAAGTTATGTAACGCGGAACTCCATATATGGGCTATGAACTAATGACCCCGTAATTGATTACTATTAATAACTAGTCAATAATCAATGTCAACGCGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGCAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTC
质粒元件
ori 1..589
/direction=RIGHT
/note="高拷贝数ColE1/pMB1/pBR322/pUC复制起点"
SV40 ori 824..959
/note="SV40复制起点"
SV40 poly(A) signal 1417..1551
/note="SV40聚腺苷酸化信号"
SV40 NLS complement(1823..1843)
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/translation="PKKKRKV"
intron 1973..2038
/note="SV40（猿猴病毒40）小t抗原内含子"
env complement(2751..4715)
/codon_start=1
/gene="env"
/product="murine leukemia virus gPr80 envelope polyprotein
precursor"
/note="蛋白水解切割成gp70SU和p15TM亚基"
/translation="MARSTLSKPPQDKINPWKPLIVMGVLLGVGMAESPHQVFNVTWRV
TNLMTGRTANATSLLGTVQDAFPKLYFDLCDLVGEEWDPSDQEPYVGYGCKYPAGRQRT
RTFDFYVCPGHTVKSGCGGPGEGYCGKWGCETTGQAYWKPTSSWDLISLKRGNTPWDTG
CSKVACGPCYDLSKVSNSFQGATRGGRCNPLVLEFTDAGKKANWDGPKSWGLRLYRTGT
DPITMFSLTRQVLNVGPRVPIGPNPVLPDQRLPSSPIEIVPAPQPPSPLNTSYPPSTTS
TPSTSPTSPSVPQPPPGTGDRLLALVKGAYQALNLTNPDKTQECWLCLVSGPPYYEGVA
VVGTYTNHSTAPANCTATSQHKLTLSEVTGQGLCMGAVPKTHQALCNTTQSAGSGSYYL
AAPAGTMWACSTGLTPCLSTTVLNLTTDYCVLVELWPRVIYHSPDYMYGQLEQRTKYKR
EPVSLTLALLLGGLTMGGIAAGIGTGTTALIKTQQFEQLHAAIQTDLNEVEKSITNLEK
SLTSLSEVVLQNRRGLDLLFLKEGGLCAALKEECCFYADHTGLVRDSMAKLRERLNQRQ
KLFETGQGWFEGLFNRSPWFTTLISTIMGPLIVLLLILLFGPCILNRLVQFVKDRISVV
QALVLTQQYHQLKPIEYEP"
RBS 3712..3720
/note="大肠杆菌起始密码子上游核糖体结合位点的完整一致序列；与16S rRNA 3'末端的区域互补（Chen等人，1994）"
gag (truncated) complement(9502..9918)
/note="缺失起始密码子的截短型莫洛尼小鼠白血病病毒gag基因"
CMV promoter complement(10280..10483)
/note="人巨细胞病毒（CMV）即刻早期启动子"
CMV enhancer 10484..10863
/note="人类巨细胞病毒即刻早期增强子"
AmpR promoter 11273..11377
/gene="bla"
AmpR 11378..12238
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="对氨苄青霉素、羧苄青霉素和相关抗生素产生耐药性"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
