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pTRV2

产品货号 P1181
产品别称 DB00097;
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价格： ¥480.00
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基本信息

产品简介

产品声明

  1. For Research Use Only
    All products available through the MiaoLing Plasmid Platform are intended for research use only. They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.
  2. Plasmid Sequence and Experimental Performance
    Plasmid products are provided and sold based on the corresponding reference/template plasmid. MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.
  3. Sequence Verification of Certain Vector Products
    For certain vector products, even the original manufacturer may not have fully verified the complete plasmid sequence. If the sequence of the key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.
  4. After-Sales Period for Small-Pack Plasmid Products
    The after-sales service period for small-pack plasmid products is three months from the date of receipt. Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.
  5. Research-Related Risks and Limitation of Liability
    Scientific research is inherently exploratory and involves a significant degree of uncertainty. To the maximum extent permitted by applicable law, MiaoLing shall not be liable for any additional economic losses, indirect losses, or other liabilities arising from the use of our products or from research outcomes.
  6. Citation in Chinese Publications
    For publications in Chinese, please cite the product as follows:
    产品名称（产品货号）由淼灵质粒平台提供。
  7. Citation in English Publications
    For publications in English, please cite the product as follows:
    “Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”

相关论文

1、 Gaofei Fu; Yulu Ma; Lin Li; Yue Wang; Shengdan Wu; Haiyang Guo; Linjing Zhang,Transcriptome analysis revealed water balance controlled by ZxOSCA1. 1 and lateral root development are crucial strategies for Zygophyllum xanthoxylon,2023-7-20,DOI:10.1016/j.sajb.2023.07.010,(IF:2.7)
2、 Yaoguang Sun;Chunxin Liu;Zengbing Liu;Tingting Zhao;Jingbin Jiang;Jingfu Li;Xiangyang Xu;Huanhuan Yang,Genome-wide identification, characterization and expression analysis of the JAZ gene family in resistance to gray leaf spots in tomato,2021-9-15,DOI:10.3390/ijms22189974,(IF:4.9)

产品文件

P1181_测序报告.zip

质粒元件

TRV strain ppk20 RNA2 5'-sequence 1..1639 TRV strain ppk20 RNA2 3'-sequence 1709..2103 6xHis 2164..2181 /codon_start=1 /product="6xHis affinity tag" /translation="HHHHHH" terminator 2216..2468 /note="胭脂碱合酶终止子和poly（A）信号" RB T-DNA repeat 2490..2514 /note="胭脂碱C58 T-DNA的右边界重复序列" CDS 3814..4443 /codon_start=1 /product="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" CDS 4877..5944 /codon_start=1 /product="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV MRYRNLIEGEASAGS" pVS1 oriV 6010..6204 /note="假单胞菌质粒pVS1的复制起点（Heeb等人，2000）" bom 6548..6688 /note="pBR322迁移区的基础" ori complement(6874..7462) /direction=LEFT /note="高拷贝数ColE1/pMB1/pBR322/pUC复制起点" KanR complement(7549..8343) /codon_start=1 /gene="aphA-3" /product="aminoglycoside phosphotransferase" /note="赋予对卡那霉素的抗性" /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" LB T-DNA repeat 8768..8792 /note="胭脂碱C58 T-DNA的左边界重复序列" CaMV 35S promoter from pCASS2 8895..9663
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