产品声明
For Research Use Only research use only . They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.Plasmid Sequence and Experimental Performance reference/template plasmid . MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.Sequence Verification of Certain Vector Products key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.After-Sales Period for Small-Pack Plasmid Products three months from the date of receipt . Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.Research-Related Risks and Limitation of Liability Citation in Chinese Publications 产品名称（产品货号）由淼灵质粒平台提供。 Citation in English Publications “Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”
质粒序列
一键复制序列（有部分质粒是非全长序列）
GAATTCCTCGAGTACGTAGGATCCATTTAAATTCTAGAGGCGCGCCGATATCCTCTCTTAAGGTAGCGAGCTCTTAATTAATAGGGATAACAGGGTAATTAACTATAACGGTCCTAAGGTAGCGATGGCAAACAGCTATTATGGGTATTATGGGTGGTTCTTTATGCGGACACTGACGGCTTTATGCCTGCAGGTCGCGAGCGATCGCGGTACCGCCCGGGCGTCGACAGGCCTGATCTAGTAACATAGATGACACCGCGCGCGATAATTTATCCTAGTTTGCGCGCTATATTTTGTTTTCTATCGCGTATTAAATGTATAATTGCGGGACTCTAATCATAAAAACCCATCTCATAAATAACGTCATGCATTACATGTTAATTATTACATGCTTAACGTAATTCAACAGAAATTATATGATAATCATCGCAAGACCGGCAACAGGATTCAATCTTAAGAAACTTTATTGCCAAATGTTTGAACGATCTGCAGTCACTTCTTCTTCTTAGCCTGTCCAGCCTTCTTGGTAGCAGCTGGTCTCTTATCTCCTCCAAGCTGAGAAAGATCGATTCTGGTCTCGTAAAGTCCGGTGATAGACTGGTGGATAAGGGTAGCATCAAGAACCTCCTTGGTAGAGGTGTATCTTTTTCTATCGATGGTGGTATCGAAGTACTTGAAAGCAGCTGGAGCACCAAGGTTGGTAAGGGTGAAAAGGTGGATGATGTTCTCAGCCTGCTCTCTGATTGGCTTATCTCTGTGCTTGTTGTAAGCAGAAAGAACCTTATCAAGGTTAGCATCAGCAAGGATAACTCTCTTAGAGAACTCAGAGATTTGCTCGATGATCTCATCAAGGTAGTGCTTGTGCTGCTCAACGAAAAGCTGCTTCTGCTCGTTATCCTCTGGAGATCCCTTCAACTTCTCGTAGTGAGAAGCAAGGTAAAGGAAGTTAACGTACTTAGATGGAAGAGCAAGCTCGTTTCCCTTCTGAAGCTCTCCAGCAGAAGCAAGCATTCTCTTTCTTCCGTTCTCAAGCTCGAAAAGAGAGTACTTTGGCAACTTGATGATAAGATCCTTCTTAACCTCCTTGTATCCCTTAGCCTCAAGGAAATCGATTGGGTTCTTCTCGAAAGAAGAACGCTCCATGATGGTGATTCCAAGAAGCTCCTTAACAGACTTCAACTTCTTAGACTTTCCCTTCTCAACCTTAGCAACAACAAGAACAGAGTAAGCAACGGTTGGAGAATCGAATCCTCCGTACTTCTTTGGGTCCCAATCCTTCTTTCTAGCGATCAACTTATCAGAGTTTCTCTTTGGAAGGATAGACTCCTTAGAGAATCCTCCGGTCTGAACCTCGGTTTTCTTAACGATGTTAACCTGTGGCATAGAAAGAACCTTTCTAACGGTAGCGAAATCTCTTCCCTTATCCCAAACGATCTCTCCGGTCTCTCCGTTGGTCTCGATAAGTGGTCTCTTTCTGATCTCTCCGTTAGCAAGGGTGATCTCGGTCTTGAAGAAGTTCATGATGTTAGAGTAGAAGAAGTACTTAGCGGTAGCCTTTCCGATCTCCTGCTCAGACTTAGCGATCATCTTTCTAACATCGTAAACCTTGTAATCTCCGTAAACGAACTCAGACTCCAACTTTGGGTACTTCTTGATAAGAGCGGTTCCAACAACAGCGTTAAGGTAAGCATCGTGAGCGTGGTGGTAGTTGTTGATCTCTCTAACCTTGTAGAACTGGAAATCCTTTCTGAAATCAGAAACCAACTTAGACTTCAAGGTGATAACCTTAACCTCTCTGATCAACTTATCGTTCTCATCGTACTTGGTGTTCATACGAGAATCAAGGATCTGAGCAACGTGCTTGGTGATCTGTCTGGTCTCAACAAGCTGTCTCTTGATGAATCCAGCCTTATCAAGCTCAGAAAGTCCTCCTCTCTCAGCCTTGGTAAGGTTATCGAACTTTCTCTGGGTGATCAACTTAGCGTTAAGAAGCTGTCTCCAGTAGTTCTTCATCTTCTTAACAACCTCCTCAGATGGAACGTTATCAGACTTTCCTCTGTTCTTATCAGAACGGGTAAGAACCTTGTTATCGATAGAATCATCCTTCAAGAAAGACTGTGGAACGATGTGATCAACATCGTAATCAGAAAGTCTGTTGATGTCAAGCTCCTGATCAACGTACATATCTCTTCCGTTCTGAAGGTAGTAAAGGTACAACTTCTCGTTCTGAAGCTGGGTGTTCTCAACTGGGTGCTCCTTCAAGATTTGAGATCCAAGCTCCTTGATTCCCTCCTCGATTCTCTTCATTCTCTCACGAGAGTTCTTCTGTCCCTTCTGGGTGGTCTGGTTCTCTCTAGCCATCTCGATAACGATGTTCTCTGGCTTGTGTCTACCCATAACCTTAACAAGCTCATCAACAACCTTAACGGTCTGAAGGATTCCCTTCTTGATAGCTGGAGATCCAGCAAGGTTAGCGATGTGCTCGTGAAGAGAATCTCCCTGTCCAGAAACCTGAGCCTTCTGGATGTCCTCCTTGAAGGTAAGAGAATCATCGTGGATAAGCTGCATGAAGTTTCTGTTAGCGAATCCATCAGACTTCAAGAAATCAAGGATGGTCTTTCCAGACTGCTTATCTCTGATTCCGTTGATCAACTTACGAGAAAGTCTACCCCATCCGGTGTATCTTCTTCTCTTCAACTGCTTCATAACCTTATCATCGAAAAGGTGAGCGTAGGTCTTGAGTCTCTCCTCGATCATCTCTCTATCCTCGAAAAGGGTAAGGGTAAGAACGATGTCCTCAAGGATGTCCTCGTTCTCCTCGTTATCAAGGAAATCCTTATCCTTGATGATCTTCAAAAGATCGTGGTAGGTTCCAAGAGAAGCGTTGAATCTATCCTCAACTCCAGAGATTTCAACAGAATCGAAGCACTCGATCTTCTTGAAGTAATCCTCCTTCAACTGCTTAACGGTAACCTTTCTGTTGGTCTTGAAAAGAAGATCAACGATAGCCTTCTTCTGCTCTCCAGAAAGGAAAGCTGGCTTTCTCATTCCCTCGGTAACGTACTTAACCTTGGTAAGCTCGTTGTAAACGGTGAAGTACTCGTAAAGAAGAGAGTGCTTTGGAAGAACCTTCTCGTTTGGAAGGTTCTTATCGAAGTTGGTCATTCTCTCGATGAAAGACTGAGCAGAAGCTCCCTTATCAACAACCTGCACATCAACAAATTTTGGTCATATATTAGAAAAGTTATAAATTAAAATATACACACTTATAAACTACAGAAAAGCAATTGCTATATACTACATTCTTTTATTTTGAAAAAAATATTTGAAATATTATATTACTACTAATTAATGATAATTATTATATATATATCAAAGGTAGAAGCAGAAACTTACCTCCTCGAAGTTCCAAGGGGTGATGGTCTCCTCAGACTTTCTGGTCATCCAAGCGAAACGAGAGTTTCCTCTAGCAAGTGGTCCAACGTAGTATGGGATTCTGAAGGTAAGGATCTTCTCGATCTTCTCTCTGTTATCCTTCAAGAATGGGTAGAAATCCTCCTGTCTACGAAGGATAGCGTGAAGCTCTCCAAGGTGGATCTGGTGTGGGATAGATCCGTTATCGAAGGTTCTCTGCTTTCTAAGAAGATCCTCTCTGTTCAACTTAACAAGAAGCTCCTCGGTTCCATCCATCTTCTCAAGGATTGGCTTGATGAACTTGTAGAACTCCTCCTGAGAAGCTCCTCCATCGATGTATCCAGCGTATCCGTTCTTAGACTGATCGAAGAAGATTTCCTTGTACTTCTCTGGAAGCTGCTGTCTAACAAGAGCCTTCAAAAGGGTAAGATCCTGGTGGTGCTCATCGTATCTCTTGATCATAGAAGCAGAAAGTGGAGCCTTGGTGATCTCGGTGTTAACTCTAAGGATGTCAGAAAGAAGGATAGCATCAGAAAGGTTCTTAGCAGCAAGGAAAAGATCAGCGTACTGATCTCCGATCTGAGCAAGAAGGTTATCAAGATCATCATCGTAGGTATCCTTAGAAAGCTGCAACTTAGCATCCTCAGCAAGATCGAAGTTAGACTTGAAGTTTGGGGTAAGTCCAAGAGAAAGAGCGATAAGGTTTCCGAAAAGTCCGTTCTTCTTCTCTCCTGGAAGCTGAGCGATAAGGTTCTCAAGTCTACGAGACTTAGAAAGTCTAGCAGAAAGGATAGCCTTAGCATCAACTCCAGAAGCGTTGATTGGGTTCTCCTCGAAAAGCTGGTTGTAGGTCTGAACAAGCTGGATGAACAACTTATCAACATCAGAGTTATCTGGGTTAAGGTCTCCCTCGATAAGGAAGTGTCCTCTGAACTTGATCATGTGAGCAAGAGCAAGGTAGATAAGTCTAAGATCAGCCTTATCGGTAGAATCAACCAACTTCTTTCTAAGGTGGTAGATGGTTGGGTACTTCTCGTGGTAAGCAACCTCATCAACGATGTTTCCGAAGATTGGGTGTCTCTCGTGCTTCTTATCCTCCTCAACAAGGAAAGACTCCTCAAGTCTGTGGAAGAAAGAATCATCAACCTTAGCCATCTCGTTAGAGAAGATTTCCTGAAGGTAGCAGATTCTGTTCTTTCTTCTGGTGTATCTTCTTCTAGCGGTTCTCTTCAATCTGGTAGCCTCAGCGGTCTCTCCAGAATCGAAAAGAAGAGCACCGATAAGGTTCTTCTTGATAGAGTGTCTATCGGTGTTTCCAAGAACCTTGAACTTCTTAGATGGAACCTTGTACTCATCGGTGATAACAGCCCATCCAACAGAGTTGGTTCCGATGTCAAGTCCGATAGAGTACTTCTTATCAGCAGCTGGAACTCCGTGGATTCCAACCTTTCTCTTCTTCTTTGGAGCCATCTTATCATCATCATCCTTGTAATCCTTATCATCATCATCCTTGTAATCCATGGATCCACGGAGCAAGGGGAGAGGGAGCCGAGAGCTGCTTACTACGTGACCGCCCCGCGCCTGCTGCTGTCCGGAGCTCCAACGGAGCTGGAGAGGGGGTGTGTGTGGCGAGAGAGTACAGCGAAAAGGTGAGAGGCGAACGGGTGAATCCTGCACGCGGAATGCCTTTATTCTTATACCATCCGTGGGGCGCGACCCAAGCTTGGGCCCAAGCTTGGGGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTTGTGGGTGGGGGTCCATCTTTGGGACCACTGTCGGCAGAGGCATCTTGAACGATAGCCTTTCCTTTATCGCAATGATGGCATTTGTAGGTGCCACCTTCCTTTTCTACTGTCCTTTTGATGAAGTGACAGATAGCTGGGCAATGGAATCCGAGGAGGTTTCCCGATATTACCCTTTGTTGAAAAGTCTCAATAGCCCTTTGGTCTTCTGAGACTGTATCTTTGATATTCTTGGAGTAAGGCCTAAGCTTGGCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGCTAGAGCAGCTTGAGCTTGGATCAGATTGTCGTTTCCCGCCTTCAGTTTAAACTATCAGTGTTTGACAGGATATATTGGCGGGTAAACCTAAGAGAAAAGAGCGTTTATTAGAATAATCGGATATTTAAAAGGGCGTGAAAAGGTTTATCCGTTCGTCCATTTGTATGTGCATGCCAACCACAGGGTTCCCCTCGGGATCAAAGTACTTTGATCCAACCCCTCCGCTGCTATAGTGCAGTCGGCTTCTGACGTTCAGTGCAGCCGTCTTCTGAAAACGACATGTCGCACAAGTCCTAAGTTACGCGACAGGCTGCCGCCCTGCCCTTTTCCTGGCGTTTTCTTGTCGCGTGTTTTAGTCGCATAAAGTAGAATACTTGCGACTAGAACCGGAGACATTACGCCATGAACAAGAGCGCCGCCGCTGGCCTGCTGGGCTATGCCCGCGTCAGCACCGACGACCAGGACTTGACCAACCAACGGGCCGAACTGCACGCGGCCGGCTGCACCAAGCTGTTTTCCGAGAAGATCACCGGCACCAGGCGCGACCGCCCGGAGCTGGCCAGGATGCTTGACCACCTACGCCCTGGCGACGTTGTGACAGTGACCAGGCTAGACCGCCTGGCCCGCAGCACCCGCGACCTACTGGACATTGCCGAGCGCATCCAGGAGGCCGGCGCGGGCCTGCGTAGCCTGGCAGAGCCGTGGGCCGACACCACCACGCCGGCCGGCCGCATGGTGTTGACCGTGTTCGCCGGCATTGCCGAGTTCGAGCGTTCCCTAATCATCGACCGCACCCGGAGCGGGCGCGAGGCCGCCAAGGCCCGAGGCGTGAAGTTTGGCCCCCGCCCTACCCTCACCCCGGCACAGATCGCGCACGCCCGCGAGCTGATCGACCAGGAAGGCCGCACCGTGAAAGAGGCGGCTGCACTGCTTGGCGTGCATCGCTCGACCCTGTACCGCGCACTTGAGCGCAGCGAGGAAGTGACGCCCACCGAGGCCAGGCGGCGCGGTGCCTTCCGTGAGGACGCATTGACCGAGGCCGACGCCCTGGCGGCCGCCGAGAATGAACGCCAAGAGGAACAAGCATGAAACCGCACCAGGACGGCCAGGACGAACCGTTTTTCATTACCGAAGAGATCGAGGCGGAGATGATCGCGGCCGGGTACGTGTTCGAGCCGCCCGCGCACGTCTCAACCGTGCGGCTGCATGAAATCCTGGCCGGTTTGTCTGATGCCAAGCTGGCGGCCTGGCCGGCCAGCTTGGCCGCTGAAGAAACCGAGCGCCGCCGTCTAAAAAGGTGATGTGTATTTGAGTAAAACAGCTTGCGTCATGCGGTCGCTGCGTATATGATGCGATGAGTAAATAAACAAATACGCAAGGGGAACGCATGAAGGTTATCGCTGTACTTAACCAGAAAGGCGGGTCAGGCAAGACGACCATCGCAACCCATCTAGCCCGCGCCCTGCAACTCGCCGGGGCCGATGTTCTGTTAGTCGATTCCGATCCCCAGGGCAGTGCCCGCGATTGGGCGGCCGTGCGGGAAGATCAACCGCTAACCGTTGTCGGCATCGACCGCCCGACGATTGACCGCGACGTGAAGGCCATCGGCCGGCGCGACTTCGTAGTGATCGACGGAGCGCCCCAGGCGGCGGACTTGGCTGTGTCCGCGATCAAGGCAGCCGACTTCGTGCTGATTCCGGTGCAGCCAAGCCCTTACGACATATGGGCCACCGCCGACCTGGTGGAGCTGGTTAAGCAGCGCATTGAGGTCACGGATGGAAGGCTACAAGCGGCCTTTGTCGTGTCGCGGGCGATCAAAGGCACGCGCATCGGCGGTGAGGTTGCCGAGGCGCTGGCCGGGTACGAGCTGCCCATTCTTGAGTCCCGTATCACGCAGCGCGTGAGCTACCCAGGCACTGCCGCCGCCGGCACAACCGTTCTTGAATCAGAACCCGAGGGCGACGCTGCCCGCGAGGTCCAGGCGCTGGCCGCTGAAATTAAATCAAAACTCATTTGAGTTAATGAGGTAAAGAGAAAATGAGCAAAAGCACAAACACGCTAAGTGCCGGCCGTCCGAGCGCACGCAGCAGCAAGGCTGCAACGTTGGCCAGCCTGGCAGACACGCCAGCCATGAAGCGGGTCAACTTTCAGTTGCCGGCGGAGGATCACACCAAGCTGAAGATGTACGCGGTACGCCAAGGCAAGACCATTACCGAGCTGCTATCTGAATACATCGCGCAGCTACCAGAGTAAATGAGCAAATGAATAAATGAGTAGATGAATTTTAGCGGCTAAAGGAGGCGGCATGGAAAATCAAGAACAACCAGGCACCGACGCCGTGGAATGCCCCATGTGTGGAGGAACGGGCGGTTGGCCAGGCGTAAGCGGCTGGGTTGCCTGCCGGCCCTGCAATGGCACTGGAACCCCCAAGCCCGAGGAATCGGCGTGAGCGGTCGCAAACCATCCGGCCCGGTACAAATCGGCGCGGCGCTGGGTGATGACCTGGTGGAGAAGTTGAAGGCCGCGCAGGCCGCCCAGCGGCAACGCATCGAGGCAGAAGCACGCCCCGGTGAATCGTGGCAAGCGGCCGCTGATCGAATCCGCAAAGAATCCCGGCAACCGCCGGCAGCCGGTGCGCCGTCGATTAGGAAGCCGCCCAAGGGCGACGAGCAACCAGATTTTTTCGTTCCGATGCTCTATGACGTGGGCACCCGCGATAGTCGCAGCATCATGGACGTGGCCGTTTTCCGTCTGTCGAAGCGTGACCGACGAGCTGGCGAGGTGATCCGCTACGAGCTTCCAGACGGGCACGTAGAGGTTTCCGCAGGGCCGGCCGGCATGGCCAGTGTGTGGGATTACGACCTGGTACTGATGGCGGTTTCCCATCTAACCGAATCCATGAACCGATACCGGGAAGGGAAGGGAGACAAGCCCGGCCGCGTGTTCCGTCCACACGTTGCGGACGTACTCAAGTTCTGCCGGCGAGCCGATGGCGGAAAGCAGAAAGACGACCTGGTAGAAACCTGCATTCGGTTAAACACCACGCACGTTGCCATGCAGCGTACGAAGAAGGCCAAGAACGGCCGCCTGGTGACGGTATCCGAGGGTGAAGCCTTGATTAGCCGCTACAAGATCGTAAAGAGCGAAACCGGGCGGCCGGAGTACATCGAGATCGAGCTAGCTGATTGGATGTACCGCGAGATCACAGAAGGCAAGAACCCGGACGTGCTGACGGTTCACCCCGATTACTTTTTGATCGATCCCGGCATCGGCCGTTTTCTCTACCGCCTGGCACGCCGCGCCGCAGGCAAGGCAGAAGCCAGATGGTTGTTCAAGACGATCTACGAACGCAGTGGCAGCGCCGGAGAGTTCAAGAAGTTCTGTTTCACCGTGCGCAAGCTGATCGGGTCAAATGACCTGCCGGAGTACGATTTGAAGGAGGAGGCGGGGCAGGCTGGCCCGATCCTAGTCATGCGCTACCGCAACCTGATCGAGGGCGAAGCATCCGCCGGTTCCTAATGTACGGAGCAGATGCTAGGGCAAATTGCCCTAGCAGGGGAAAAAGGTCGAAAAGGTCTCTTTCCTGTGGATAGCACGTACATTGGGAACCCAAAGCCGTACATTGGGAACCGGAACCCGTACATTGGGAACCCAAAGCCGTACATTGGGAACCGGTCACACATGTAAGTGACTGATATAAAAGAGAAAAAAGGCGATTTTTCCGCCTAAAACTCTTTAAAACTTATTAAAACTCTTAAAACCCGCCTGGCCTGTGCATAACTGTCTGGCCAGCGCACAGCCGAAGAGCTGCAAAAAGCGCCTACCCTTCGGTCGCTGCGCTCCCTACGCCCCGCCGCTTCGCGTCGGCCTATCGCGGCCGCTGGCCGCTCAAAAATGGCTGGCCTACGGCCAGGCAATCTACCAGGGCGCGGACAAGCCGCGCCGTCGCCACTCGACCGCCGGCGCCCACATCAAGGCACCCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGCATTCTAGGTACTAAAACAATTCATCCAGTAAAATATAATATTTTATTTTCTCCCAATCAGGCTTGATCCCCAGTAAGTCAAAAAATAGCTCGACATACTGTTCTTCCCCGATATCCTCCCTGATCGACCGGACGCAGAAGGCAATGTCATACCACTTGTCCGCCCTGCCGCTTCTCCCAAGATCAATAAAGCCACTTACTTTGCCATCTTTCACAAAGATGTTGCTGTCTCCCAGGTCGCCGTGGGAAAAGACAAGTTCCTCTTCGGGCTTTTCCGTCTTTAAAAAATCATACAGCTCGCGCGGATCTTTAAATGGAGTGTCTTCTTCCCAGTTTTCGCAATCCACATCGGCCAGATCGTTATTCAGTAAGTAATCCAATTCGGCTAAGCGGCTGTCTAAGCTATTCGTATAGGGACAATCCGATATGTCGATGGAGTGAAAGAGCCTGATGCACTCCGCATACAGCTCGATAATCTTTTCAGGGCTTTGTTCATCTTCATACTCTTCCGAGCAAAGGACGCCATCGGCCTCACTCATGAGCAGATTGCTCCAGCCATCATGCCGTTCAAAGTGCAGGACCTTTGGAACAGGCAGCTTTCCTTCCAGCCATAGCATCATGTCCTTTTCCCGTTCCACATCATAGGTGGTCCCTTTATACCGGCTGTCCGTCATTTTTAAATATAGGTTTTCATTTTCTCCCACCAGCTTATATACCTTAGCAGGAGACATTCCTTCCGTATCTTTTACGCAGCGGTATTTTTCGATCAGTTTTTTCAATTCCGGTGATATTCTCATTTTAGCCATTTATTATTTCCTTCCTCTTTTCTACAGTATTTAAAGATACCCCAAGAAGCTAATTATAACAAGACGAACTCCAATTCACTGTTCCTTGCATTCTAAAACCTTAAATACCAGAAAACAGCTTTTTCAAAGTTGTTTTCAAAGTTGGCGTATAACATAGTATCGACGGAGCCGATTTTGAAACCGCGGTGATCACAGGCAGCAACGCTCTGTCATCGTTACAATCAACATGCTACCCTCCGCGAGATCATCCGTGTTTCAAACCCGGCAGCTTAGTTGCCGTTCTTCCGAATAGCATCGGTAACATGAGCAAAGTCTGCCGCCTTACAACGGCTCTCCCGCTGACGCCGTCCCGGACTGATGGGCTGCCTGTATCGAGTGGTGATTTTGTGCCGAGCTGCCGGTCGGGGAGCTGTTGGCTGGCTGGTGGCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGAATTAACGCCGAATTAATTCGAGCTCGGATCTGATAATTTATTTGAAAATTCATAAGAAAAGCAAACGTTACATGAATTGATGAAACAATACAAAGACAGATAAAGCCACGCACATTTAGGATATTGGCCGAGATTACTGAATATTGAGTAAGATCACGGAATTTCTGACAGGAGCATGTCTTCAATTCAGCCCAAATGGCAGTTGAAATACTCAAACCGCCCCATATGCAGGAGCGGATCATTCATTGTTTGTTTGGTTGCCTTTGCCAACATGGGAGTCCAAGATTCTGCAGTCAAATCTCGGTGACGGGCAGGACCGGACGGGGCGGTACCGGCAGGCTGAAGTCCAGCTGCCAGAAACCCACGTCATGCCAGTTCCCGTGCTTGAAGCCGGCCGCCCGCAGCATGCCGCGGGGGGCATATCCGAGCGCCTCGTGCATGCGCACGCTCGGGTCGTTGGGCAGCCCGATGACAGCGACCACGCTCTTGAAGCCCTGTGCCTCCAGGGACTTCAGCAGGTGGGTGTAGAGCGTGGAGCCCAGTCCCGTCCGCTGGTGGCGGGGGGAGACGTACACGGTCGACTCGGCCGTCCAGTCGTAGGCGTTGCGTGCCTTCCAGGGGCCCGCGTAGGCGATGCCGGCGACCTCGCCGTCCACCTCGGCGACGAGCCAGGGATAGCGCTCCCGCAGACGGACGAGGTCGTCCGTCCACTCCTGCGGTTCCTGCGGCTCGGTACGGAAGTTGACCGTGCTTGTCTCGATGTAGTGGTTGACGATGGTGCAGACCGCCGGCATGTCCGCCTCGGTGGCACGGCGGATGTCGGCCGGGCGTCGTTCTGGGCTCATCGATTCGATTTGGTGTATCGAGATTGGTTATGAAATTCAGATGCTAGTGTAATGTATTGGTAATTTGGGAAGATATAATAGGAAGCAAGGCTATTTATCCATTTCTGAAAAGGCGAAATGGCGTCACCGCGAGCGTCACGCGCATTCCGTTCTTGCTGTAAAGCGTTGTTTGGTACACTTTTGACTAGCGAGGCTTGGCGTGTCAGCGTATCTATTCAAAAGTCGTTAATGGCTGCGGATCAAGAAAAAGTTGGAATAGAAACAGAATACCCGCGAAATTCAGGCCCGGTTGCCATGTCCTACACGCCGAAATAAACGACCAAATTAGTAGAAAAATAAAAACTGACTCGGATACTTACGTCACGTCTTGCGCACTGATTTGAAAAATCTCA
质粒元件
terminator 235..487
/note="胭脂碱合酶终止子和poly（A）信号"
nucleoplasmin NLS complement(496..543)
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/translation="KRPAATKKAGQAKKKK"
CDS complement(join(544..3207,3397..4833))
/codon_start=1
/product="Cas9 endonuclease from the Streptococcus pyogenes
Type II CRISPR/Cas system"
/note="含有马铃薯IV2内含子的植物密码子优化Cas9基因"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
intron 3208..3396
/note="马铃薯ST-LS1基因第二内含子的修饰（Vananneyt等人，1990）"
SV40 NLS complement(4858..4878)
/codon_start=1
/product="nuclear localization signal of SV40 large T
antigen"
/translation="PKKKRKV"
2xFLAG complement(4885..4932)
/codon_start=1
/product="two tandem FLAG(R) epitope tags"
/translation="DYKDDDDKDYKDDDDK"
35SPPDK promoter complement(4944..5453)
/note="由花椰菜花叶病毒35S增强子与玉米C4PPDK基础启动子融合而成的杂交启动子（Yoo等人，2007）"
primer_bind complement(5516..5532)
/note="常见的测序引物，多种相似变体之一"
RB T-DNA repeat 5735..5759
/note="胭脂碱C58 T-DNA的右边界重复序列"
CDS 7060..7689
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 8118..9191
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
pVS1 oriV 9257..9451
/note="假单胞菌质粒pVS1的复制起点（Heeb等人，2000）"
bom 9795..9935
/note="pBR322迁移区的基础"
ori complement(10121..10709)
/direction=LEFT
/note="高拷贝数ColE1/pMB1/pBR322/pUC复制起点"
KanR complement(10796..11590)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/note="赋予对卡那霉素的抗性"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
LB T-DNA repeat 12015..12039
/note="胭脂碱C58 T-DNA的左边界重复序列"
terminator 12125..12377
/note="甘露碱合酶终止子"
BlpR complement(12387..12938)
/codon_start=1
/gene="Streptomyces hygroscopicus bar"
/product="phosphinothricin acetyltransferase"
/note="赋予对双硫磷或草丁膦的抗性"
/translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
WQLDFSLPVPPRPVLPVTEI"
MAS promoter complement(12944..13324)
/note="甘露聚糖合酶启动子（Velten等人，1984）"
