中文 / EN
首页 / 产品与服务 / 全部现货质粒

pLV3-EnCMV-Macc1(mouse)-EF1a-Hyg

产品货号 P106374
产品别称 NM_001163136.1;4732474O15Rik; Gm267;
选择规格
数　量
转染级质粒质检标准 查看质粒手册
价格： ¥380.00
联系我们

基本信息

产品简介

产品声明

  1. For Research Use Only
    All products available through the MiaoLing Plasmid Platform are intended for research use only. They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.
  2. Plasmid Sequence and Experimental Performance
    Plasmid products are provided and sold based on the corresponding reference/template plasmid. MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.
  3. Sequence Verification of Certain Vector Products
    For certain vector products, even the original manufacturer may not have fully verified the complete plasmid sequence. If the sequence of the key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.
  4. After-Sales Period for Small-Pack Plasmid Products
    The after-sales service period for small-pack plasmid products is three months from the date of receipt. Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.
  5. Research-Related Risks and Limitation of Liability
    Scientific research is inherently exploratory and involves a significant degree of uncertainty. To the maximum extent permitted by applicable law, MiaoLing shall not be liable for any additional economic losses, indirect losses, or other liabilities arising from the use of our products or from research outcomes.
  6. Citation in Chinese Publications
    For publications in Chinese, please cite the product as follows:
    产品名称（产品货号）由淼灵质粒平台提供。
  7. Citation in English Publications
    For publications in English, please cite the product as follows:
    “Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”

相关论文

暂无相关文献

产品文件

P106374_测序报告.zip P106374_全序列图谱.dna

质粒元件

    5' LTR (truncated)             234..414
                     /note="来自HIV-1的截短型5'长末端重复序列（LTR）"
     HIV-1 Psi    458..583
                     /note="人类免疫缺陷病毒 1 型（HIV-1）的包装信号”
    RRE    1076..1309
                     /note="HIV-1 的 Rev 反应元件（RRE）能够介导 Rev 依赖性的 mRNA 从细胞核输出到细胞质。"
     cPPT/CTS    1804..1920
                     /note="HIV-1 的中央多嘌呤区（cPPT）和中央终止序列（CTS）"
     CMV enhancer        1933..2236
                     /note="人巨细胞病毒的立即早期增强子（HCMV IE 增强子）"
     CMV promoter        2237..2440
                     /note="人巨细胞病毒（CMV）立即早期启动子"
     Macc1-m             2491..5046
                     /codon_start=1
                     /translation="MLISERAYCWPREIGRSRSEGNLIDMEASKRSKNQNITARQGLGL
                     PHNWPDAPTLHRDSDAKATNPFWKELSASNPFLEDITQLRNNKKRDNMSILKEDPFLFL
                     RQIETENSFDSSGDELDMDQGFKSSSSRKSGRSKSVSELLDVLGDRTHTHQSIHSSDQI
                     LIQDLEWLQNDREAYKMAWLSQRQLARSCLDLNTINQSPGWAQTQIAETIAVCKLNHQG
                     GSVQLPDSDITIHIPQGHIAVGEFQEVSLRAFLDPPPKLNHDLSCTVSPLLEIRLSNLN
                     TMEAILLEMKTGAEVKTDPLSQVMTKMVCLYSLDREGPFQALNTCYIYKDTIQAKLLDL
                     SHVMYIVIAVQATIIHPPAATIWDYIHKTTSIGIYGPKYLHPSFTAVFTVCGHNYVPGK
                     FTVSDIRKGGKNTSPVVFHLWGKHSFSLDKPKDLIISAFSCDPDFEVKEEGERKVIAQR
                     QLEAGVVVHQQVFFSSIDHREMHLFVFRVQVKTPNEKPVTQFFVTSPDPSPNLKRFSYL
                     PGFFQKREDKRVPLLSTGLVKYPTFQDKKLNFTNYGVALKTVLRQNKIDYLLEYLKGDT
                     IALLGEGTVRAIGQSKVKEWYVGVLRGKIGLVHCKNVKVISKEQVMSMSDGILTTKNLL
                     EQIALPFKSLTYIYSVVLTLVSEKVHDWKVLANVLGYSHLAQEDFNRIQADKEPERVSY
                     VVKKLKEDCHEDRNTRKFLYELIVALLKMDCQELVAHLIQEAVILTSAVKLGRSWRELA
                     EKSAGLTKHQMQAYEIPHRGKSGDVSAEMMWKPAYDFLYAWSFHYGNSYRDVLQDLQSA
                     LDRMKNPVTKRWRDLTGTLILINSLEILRVTAFSTSQEI"
     EF-1-alpha core promoter        5093..5304
                     /note="人延伸因子 EF-1α 的核心启动子"
     5' LTR (truncated)             5317..5585
                     /note="来自人T细胞白血病病毒1型（HTLV-1）的截短型5′长末端重复序列（LTR）"
     HygR             5613..6638
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /note="（该元件/基因）赋予对潮霉素的抗性"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     WPRE      6645..7233
                     /note="土拨鼠肝炎病毒转录后调控元件"
     Factor Xa site            complement(7116..7127)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     Cleavage site after base 7115"
                     /translation="IEGR"
    3' LTR (Delta-U3)             7307..7540
                     /note="来自 HIV-1 的自失活型 3′ 长末端重复序列（LTR）"
     SV40 poly(A) signal    7612..7733
                     /note="SV40 多聚腺苷酸化信号"
    SV40 ori      7752..7887
                     /note="SV40 复制起点"
    ori      complement(8327..8915)
                     /direction=LEFT
                     /note="高拷贝数 ColE1/pMB1/pBR322/pUC 复制起点"
     AmpR             complement(9086..9946)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="赋予对氨苄青霉素、羧苄青霉素及相关抗生素的抗性"
                     /note="This feature has 2 segments:
                        1: 9086 .. 9877 / #ccffcc
                        2: 9878 .. 9946 / #ccffcc / signal sequence
                     Cleavage site after base 9877"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     AmpR promoter        complement(9947..10051)
                     /gene="bla”
×

质粒序列

×
（有部分质粒是非全长序列）
收藏本站×

请按 Ctrl + D 键将本页加入收藏夹。

Mac 用户请按 ⌘ + D 键。