产品声明
For Research Use Only research use only . They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.Plasmid Sequence and Experimental Performance reference/template plasmid . MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.Sequence Verification of Certain Vector Products key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.After-Sales Period for Small-Pack Plasmid Products three months from the date of receipt . Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.Research-Related Risks and Limitation of Liability Citation in Chinese Publications 产品名称（产品货号）由淼灵质粒平台提供。 Citation in English Publications “Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”
质粒序列
一键复制序列（有部分质粒是非全长序列）
GAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTTTTTATTACCTACAACTTCTTTAGCACTTAAACGACCATCATCAGTTATTGGAACAACGCCATAATGCATATGTGGTGTTTTTTCGTCCATGTGAACTGTTGCATATAATAAATTATCTTTACCGTATTCTTGTTCTAAAAACTCTTTAGCATATTCAAAAAACTGCTTTGTATCTTCTGGCGTTTGATTATCAAAGAAATCATTGTCTGATGTAATTAAACCATCAATGTGTTTAATCGCGTCTGTTCTAATTTTTCTTTTGCCTGTATAATTCTGTTCGATTTTTTCATCAATCAAGTTATTAAAATTCTGTTTATTAGCATTTACCAAATCATAATTTAAGTAAGTTTTACTATGGTCTATATCTTCATTTTCATAATTATTATTTTCTCTTTGAACATGTTTTTGTATGCCCGTTGTATTTGTTCCAGATTTAACTTTTGAAACTCTAACAATGGAATAAGACATATAAAAATCACTCCTAAGCACAAAGGTTTTATGTGTATTTAATAGTTTAAGTCTAACACACTAGACTTATTTTTAATAAGTCGTTAATACGTGTGCTCTGCGAGGCTTAAACCTGTTTTTGCTAACGCAAAAAATGAGTGGCAAAATGCTAGCCACTCATAGTTCTAAACCAAAATATAATATAACTATTCAAACTGCTTTTCAGAACGTTTAAATACAATAATCGTCAAAAGACAACATAAAATAATAAGGATAGCCATGGCTACAAGAATATTACTATACACTCCAGAAGAATAATTTATAAATTCCAGAACTAGTTTACGATTAATCAATTGTAGTGACAATAAACCTCCTACAATTGCTATACCTGTTCCCTCTGATAAAAAACTTGTGAAATTTAGCAAACTCATTCCAGAAGCAACTTCTTCTTCAGAAAGACTACTTGATACTATTTTTGATATAACTGTTTTAGTAAAAGATAATCCGCCCATAACAAATATAAACATAAAAGTAGTCAACCACATACTAAACTCAACAAAAAATGCAATAGTTAAAAAACTTATAGAGATAGACAATGATCCTAAAATAAAAACAAATAATGATCCTTTTCTATCCACTAAAAAACCACCAAAATAACCAAAAACAATAACACTCATGGTTCCAGGAAAAATAACACTATTACCTATTGTCGCTACATTTACATGATAAATAGTTTTCATCATATAAGGCACCATTGATATAAAACCAGCTACTATAGAAAATATTAGCCCACCAGAAAACAAACCAAGCATAAACGGAATGTTTTTCCCTAGTTTAGGATTAATAAAAGGGTTAGAAACTCTTGAAATATGTTTAATAAAAATCACAAAAAAGATTGTGAAGAGTATTAAAAAAGTCCAATTATAATTTGTCGTAAATAACATAAAACATATAATACTTATAGACATTAAAACAATACCTACGATATCTAATGTATTTTTTGTTGATTTACCAGGTACCATTACTTTAATAAGAAAAGGTATAGTTACTATTGTAATCATAGGAAGTATAAGTAGGTAAGACCAATGAATATAATGTGCTATTATTCCCCCTATTGAAGGACCTAACCCTTCACCTAAAGCTACAATTGATCCTATAAAACCAAAGGCTTTGCCTTGTTTTTTTCTTGTAATATTTCTAGCTACAACCACCATAATCAGTGAAGGGAATGCAGCAGATCCTACTCCTTGTACTAACCTACCAAAAATCAAAATAAAAAAGTGATTGTGACCAATAAAAGCAATCAATGAACCAAGACAGCTCAAACTAATACCAATAATTAACAATTTTTTTATATTTATATAATCAGATAATTTTCCATATACTGCTGTTCCTATCGAAAAAGTTAACATATATGCAGTGTTTACCCAGTTTGTAATTCCAGGAGTAGTATTAAAATGATTTGCAATATCAGGTAAAGAAACATTTAAAACCATTTCATTTAATACACTAAAAAATGAAAGAATACAAAGCCAAAATAAAACGCTATAAAACAAACCTTTAAATTTTTTATATAAACTAAACAATTTTACCTCTTTACTTGAGGTGACTAAAGTTTATAGGGGTAAATTATTCTTTCACCTCATATAAATTCCCCAAATTTTTAAATAGACACTTCATAAAAAAATCCTCCTAAATTAGAATAGTTTAAATATATCATTTTTGTTCAGTAATATTAATATGTACACTATTTCCAAAATTTAAATTCATGTTGCCAAAAATCGATTATGTCTTTTGCGGGGCAAAAAAAGGGCAGATTATTTGAAAAAGGGCAAACGCTTGTGGAAAAGCTAAAAGGTTAAAAATGACAAAAACCTTGATACAACAGTGTTTTTGGACGCTCGTGTACGTTAGAGAATGACCGGTTTACCATCATACAAGGATGGGATTAACTTGTGTTAAAAAGCCTTTAATATCAGTTGTTACAAAGGATTTGTAGCGTCTTTAAAAATAAAAAAGGGCAGAAAAAGGGCAGATACCTTTTAGTACACAAGTTTTTCTAATTTTTGCTCTAACTCTCTGTCCATTTTCTCTGTTACATGTGTATACACCTTTATAGTCGTTTTTTCCAGATCTGGAATTAATGAGGCATTCTAACATTACAGAAACAATTCAAACGTACTCTCATTTATATACTGATAAAAAACATCAAGCTATGAGCATATTTGATTAAACGGTATCAAATTGGTATCAAATAACAATTAAGGAGTTTATAAAATGCGTAATAACAAGCCTAAAATAAGTATTCAAAACGACCCATGGGAAGTGAAATTTATATACATTTAAATTTCATGAGACAATAAACGTTGATTTAATGCGTTTTTTTGCCTTTTTTATTTTCCTTATTTTTTCTGTTTTACAACAAAATGGTATCAAAAATGGTATCATTTGTAGTTATTTTAGCTTCACATATTAAAACAACCACACTCCTAAATTAATAGGTGGTGTGGTTTTGTTGGTTGTGTGGGGATCGATAGATCTGGATCTGGATTTTAATGCGGATGTTGCGATTACTTCGCCAACTATTGCGATAACAAGAAAAAGCCAGCCTTTCATGATATATCTCCCAATTTGTGTAGGGCTTATTATGCACGCTTAAAAATAATAAAAGCAGACTTGACCTGATAGTTTGGCTGTGAGCAATTATGTGCTTAGTGCATCTAACGCTTGAGTTAAGCCGCGCCGCGAAGCGGCGTCGGCTTGAACGAATTGTTAGACATTATTTGCCGACTACCTTGGTGATCTCGCCTTTCACGTAGTGGACAAATTCTTCCAACTGATCTGCGCGCGAGGCCAAGCGATCTTCTTCTTGTCCAAGATAAGCCTGTCTAGCTTCAAGTATGACGGGCTGATACTGGGCCGGCAGGCGCTCCATTGCCCAGTCGGCAGCGACATCCTTCGGCGCGATTTTGCCGGTTACTGCGCTGTACCAAATGCGGGACAACGTAAGCACTACATTTCGCTCATCGCCAGCCCAGTCGGGCGGCGAGTTCCATAGCGTTAAGGTTTCATTTAGCGCCTCAAATAGATCCTGTTCAGGAACCGGATCAAAGAGTTCCTCCGCCGCTGGACCTACCAAGGCAACGCTATGTTCTCTTGCTTTTGTCAGCAAGATAGCCAGATCAATGTCGATCGTGGCTGGCTCGAAGATACCTGCAAGAATGTCATTGCGCTGCCATTCTCCAAATTGCAGTTCGCGCTTAGCTGGATAACGCCACGGAATGATGTCGTCGTGCACAACAATGGTGACTTCTACAGCGCGGAGAATCTCGCTCTCTCCAGGGGAAGCCGAAGTTTCCAAAAGGTCGTTGATCAAAGCTCGCCGCGTTGTTTCATCAAGCCTTACGGTCACCGTAACCAGCAAATCAATATCACTGTGTGGCTTCAGGCCGCCATCCACTGCGGAGCCGTACAAATGTACGGCCAGCAACGTCGGTTCGAGATGGCGCTCGATGACGCCAACTACCTCTGATAGTTGAGTCGATACTTCGGCGATCACCGCTTCCCTCATGATGTTTAACTTTGTTTTAGGGCGACTGCCCTGCTGCGTAACATCGTTGCTGCTCCATAACATCAAACATCGACCCACGGCGTAACGCGCTTGCTGCTTGGATGCCCGAGGCATAGACTGTACCCCAAAAAAACAGTCATAACAAGCCATGAAAACCGCCACTGCGCCGTTACCACCGCTGCGTTCGGTCAAGGTTCTGGACCAGTTGCGTGAGCGCATACGCTACTTGCATTACAGCTTACGAACCGAACAGGCTTATGTCCACTGGGTTCGTGCCTTCATCCGTTTCCACGGTGTGCGTCACCCGGCAACCTTGGGCAGCAGCGAAGTCGAGGCATTTCTGTCCTGGCTGGCGAACGAGCGCAAGGTTTCGGTCTCCACGCATCGTCAGGCATTGGCGGCCTTGCTGTTCTTCTACGGCAAGGTGCTGTGCACGGATCTGCCCTGGCTTCAGGAGATCGGAAGACCTCGGCCGTCGCGGCGCTTGCCGGTGGTGCTGACCCCGGATGAAGTGGTTCGCATCCTCGGTTTTCTGGAAGGCGAGCATCGTTTGTTCGCCCAGCTTCTGTATGGAACGGGCATGCGGATCAGTGAGGGTTTGCAACTGCGGGTCAAGGATCTGGATTTCGATCACGGCACGATCATCGTGCGGGAGGGCAAGGGCTCCAAGGATCGGGCCTTGATGTTACCCGAGAGCTTGGCACCCAGCCTGCGCGAGCAGGGGAATTAATTCCCACGGGTTTTGCTGCCCGCAAACGGGCTGTTCTGGTGTTGCTAGTTTGTTATCAGAATCGCAGATCCGGCTTCAGCCGGTTTGCCGGCTGAAAGCGCTATTTCTTCCAGAATTGCCATGATTTTTTCCCCACGGGAGGCGTCACTGGCTCCCGTGTTGTCGGCAGCTTTGATTCGATAAGCAGCATCGCCTGTTTCAGGCTGTCTATGTGTGACTGTTGAGCTGTAACAAGTTGTCTCAGGTGTTCAATTTCATGTTCTAGTTGCTTTGTTTTACTGGTTTCACCTGTTCTATTAGGTGTTACATGCTGTTCATCTGTTACATTGTCGATCTGTTCATGGTGAACAGCTTTGAATGCACCAAAAACTCGTAAAAGCTCTGATGTATCTATCTTTTTTACACCGTTTTCATCTGTGCATATGGACAGTTTTCCCTTTGATATGTAACGGTGAACAGTTGTTCTACTTTTGTTTGTTAGTCTTGATGCTTCACTGATAGATACAAGAGCCATAAGAACCTCAGATCCTTCCGTATTTAGCCAGTATGTTCTCTAGTGTGGTTCGTTGTTTTTGCGTGAGCCATGAGAACGAACCATTGAGATCATACTTACTTTGCATGTCACTCAAAAATTTTGCCTCAAAACTGGTGAGCTGAATTTTTGCAGTTAAAGCATCGTGTAGTGTTTTTCTTAGTCCGTTATGTAGGTAGGAATCTGATGTAATGGTTGTTGGTATTTTGTCACCATTCATTTTTATCTGGTTGTTCTCAAGTTCGGTTACGAGATCCATTTGTCTATCTAGTTCAACTTGGAAAATCAACGTATCAGTCGGGCGGCCTCGCTTATCAACCACCAATTTCATATTGCTGTAAGTGTTTAAATCTTTACTTATTGGTTTCAAAACCCATTGGTTAAGCCTTTTAAACTCATGGTAGTTATTTTCAAGCATTAACATGAACTTAAATTCATCAAGGCTAATCTCTATATTTGCCTTGTGAGTTTTCTTTTGTGTTAGTTCTTTTAATAACCACTCATAAATCCTCATAGAGTATTTGTTTTCAAAAGACTTAACATGTTCCAGATTATATTTTATGAATTTTTTTAACTGGAAAAGATAAGGCAATATCTCTTCACTAAAAACTAATTCTAATTTTTCGCTTGAGAACTTGGCATAGTTTGTCCACTGGAAAATCTCAAAGCCTTTAACCAAAGGATTCCTGATTTCCACAGTTCTCGTCATCAGCTCTCTGGTTGCTTTAGCTAATACACCATAAGCATTTTCCCTACTGATGTTCATCATCTGAGCGTATTGGTTATAAGTGAACGATACCGTCCGTTCTTTCCTTGTAGGGTTTTCAATCGTGGGGTTGAGTAGTGCCACACAGCATAAAATTAGCTTGGTTTCATGCTCCGTTAAGTCATAGCGACTAATCGCTAGTTCATTTGCTTTGAAAACAACTAATTCAGACATACATCTCAATTGGTCTAGGTGATTTTAATCACTATACCAATTGAGATGGGCTAGTCAATGATAATTACTAGTCCTTTTCCTTTGAGTTGTGGGTATCTGTAAATTCTGCTAGACCTTTGCTGGAAAACTTGTAAATTCTGCTAGACCCTCTGTAAATTCCGGTAGACCTTTGTGTGTTTTTTTTGTTTATATTCAAGTGGTTATAATTTATAGAATAAAGAAAGAATAAAAAAAGATAAAAAGAATAGATCCCAGCCCTGTGTATAACTCACTACTTTAGTCAGTTCCGCAGTATTACAAAAGGATGTCGCAAACGCTGTTTGCTCCTCTACAAAACAGACCTTAAAACCCTAAAGGCTTAAGTAGCACCCTCGCAAGCTCGGGCAAATCGCTGAATATTCCTTTTGTCTCCGACCATCAGGCACCTGAGTCGCTGTCTTTTTCGTGACATTCAGTTCGCTGCGCTCACGGCTCTGGCAGTGAATGGGGGTAAATGGCACTACAGGCGCCTTTTATGGATTCATGCAAGGAAACTACCCATAATACAAGAAAAGCCCGTCACGGGCTTCTCAGGGCGTTTTATGGCGGGTCTGCTATGTGGTGCTATCTGACTTTTTGCTGTTCAGCAGTTCCTGCCCTCTGATTTTCCAGTCTGACCACTTCGGATTATCCCGTGACAGGTCATTCAGACTGGCTAATGCACCCAGTAAGGCAGCGGTATCATCAACAGGCTTACCCGTCTTACTGTCGGGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTGATCCGCTGTAATGGGCCCAATCACTAGTGAATTCCCGAAGCTTAGATCTAATC
质粒元件
MCS 1..57
/note="pUC18/19多克隆位点"
primer_bind complement(533..554)
SmR complement(3285..4076)
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/note="赋予对壮观霉素和链霉素的抗性"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
CDS complement(5328..6278)
/codon_start=1
/gene="rep101"
/product="RepA protein needed for replication with the
pSC101 origin"
/translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
pSC101 ori 6326..6548
/note="需要Rep101蛋白的低拷贝复制起点"
primer_bind 6527..6547
terminator 7126..7212
/gene="Escherichia coli rrnB"
/note="大肠杆菌rrnB基因的转录终止子T1"
terminator 7309..7395
/gene="Escherichia coli rrnB"
/note="大肠杆菌rrnB基因的转录终止子T1"
terminator 7492..7578
/gene="Escherichia coli rrnB"
/note="大肠杆菌rrnB基因的转录终止子T1"
terminator 7675..7761
/gene="Escherichia coli rrnB"
/note="大肠杆菌rrnB基因的转录终止子T1"
terminator 7858..7944
/gene="Escherichia coli rrnB"
/note="大肠杆菌rrnB基因的转录终止子T1"
