pLL39

Catalog Number P10318

Alternative Names 等效质粒Plasmid#15458

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Custom Scale / Special Requirements / Custom ProjectsSmall-Scale Dry Plasmid (Requires Transformation & Preparation)

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Transfection-Grade Plasmid QC Standards View Plasmid Manual

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Plasmid Elements

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Product Overview

Product Disclaimer

For Research Use Only
All products available through the MiaoLing Plasmid Platform are intended for research use only. They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.
Plasmid Sequence and Experimental Performance
Plasmid products are provided and sold based on the corresponding reference/template plasmid. MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.
Sequence Verification of Certain Vector Products
For certain vector products, even the original manufacturer may not have fully verified the complete plasmid sequence. If the sequence of the key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.
After-Sales Period for Small-Pack Plasmid Products
The after-sales service period for small-pack plasmid products is three months from the date of receipt. Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.
Research-Related Risks and Limitation of Liability
Scientific research is inherently exploratory and involves a significant degree of uncertainty. To the maximum extent permitted by applicable law, MiaoLing shall not be liable for any additional economic losses, indirect losses, or other liabilities arising from the use of our products or from research outcomes.
Citation in Chinese Publications
For publications in Chinese, please cite the product as follows:
Product names and IDs are provided by the MiaoLing Plasmid Platform.
Citation in English Publications
For publications in English, please cite the product as follows:
“Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”

质粒序列

GAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTTTTTATTACCTACAACTTCTTTAGCACTTAAACGACCATCATCAGTTATTGGAACAACGCCATAATGCATATGTGGTGTTTTTTCGTCCATGTGAACTGTTGCATATAATAAATTATCTTTACCGTATTCTTGTTCTAAAAACTCTTTAGCATATTCAAAAAACTGCTTTGTATCTTCTGGCGTTTGATTATCAAAGAAATCATTGTCTGATGTAATTAAACCATCAATGTGTTTAATCGCGTCTGTTCTAATTTTTCTTTTGCCTGTATAATTCTGTTCGATTTTTTCATCAATCAAGTTATTAAAATTCTGTTTATTAGCATTTACCAAATCATAATTTAAGTAAGTTTTACTATGGTCTATATCTTCATTTTCATAATTATTATTTTCTCTTTGAACATGTTTTTGTATGCCCGTTGTATTTGTTCCAGATTTAACTTTTGAAACTCTAACAATGGAATAAGACATATAAAAATCACTCCTAAGCACAAAGGTTTTATGTGTATTTAATAGTTTAAGTCTAACACACTAGACTTATTTTTAATAAGTCGTTAATACGTGTGCTCTGCGAGGCTTAAACCTGTTTTTGCTAACGCAAAAAATGAGTGGCAAAATGCTAGCCACTCATAGTTCTAAACCAAAATATAATATAACTATTCAAACTGCTTTTCAGAACGTTTAAATACAATAATCGTCAAAAGACAACATAAAATAATAAGGATAGCCATGGCTACAAGAATATTACTATACACTCCAGAAGAATAATTTATAAATTCCAGAACTAGTTTACGATTAATCAATTGTAGTGACAATAAACCTCCTACAATTGCTATACCTGTTCCCTCTGATAAAAAACTTGTGAAATTTAGCAAACTCATTCCAGAAGCAACTTCTTCTTCAGAAAGACTACTTGATACTATTTTTGATATAACTGTTTTAGTAAAAGATAATCCGCCCATAACAAATATAAACATAAAAGTAGTCAACCACATACTAAACTCAACAAAAAATGCAATAGTTAAAAAACTTATAGAGATAGACAATGATCCTAAAATAAAAACAAATAATGATCCTTTTCTATCCACTAAAAAACCACCAAAATAACCAAAAACAATAACACTCATGGTTCCAGGAAAAATAACACTATTACCTATTGTCGCTACATTTACATGATAAATAGTTTTCATCATATAAGGCACCATTGATATAAAACCAGCTACTATAGAAAATATTAGCCCACCAGAAAACAAACCAAGCATAAACGGAATGTTTTTCCCTAGTTTAGGATTAATAAAAGGGTTAGAAACTCTTGAAATATGTTTAATAAAAATCACAAAAAAGATTGTGAAGAGTATTAAAAAAGTCCAATTATAATTTGTCGTAAATAACATAAAACATATAATACTTATAGACATTAAAACAATACCTACGATATCTAATGTATTTTTTGTTGATTTACCAGGTACCATTACTTTAATAAGAAAAGGTATAGTTACTATTGTAATCATAGGAAGTATAAGTAGGTAAGACCAATGAATATAATGTGCTATTATTCCCCCTATTGAAGGACCTAACCCTTCACCTAAAGCTACAATTGATCCTATAAAACCAAAGGCTTTGCCTTGTTTTTTTCTTGTAATATTTCTAGCTACAACCACCATAATCAGTGAAGGGAATGCAGCAGATCCTACTCCTTGTACTAACCTACCAAAAATCAAAATAAAAAAGTGATTGTGACCAATAAAAGCAATCAATGAACCAAGACAGCTCAAACTAATACCAATAATTAACAATTTTTTTATATTTATATAATCAGATAATTTTCCATATACTGCTGTTCCTATCGAAAAAGTTAACATATATGCAGTGTTTACCCAGTTTGTAATTCCAGGAGTAGTATTAAAATGATTTGCAATATCAGGTAAAGAAACATTTAAAACCATTTCATTTAATACACTAAAAAATGAAAGAATACAAAGCCAAAATAAAACGCTATAAAACAAACCTTTAAATTTTTTATATAAACTAAACAATTTTACCTCTTTACTTGAGGTGACTAAAGTTTATAGGGGTAAATTATTCTTTCACCTCATATAAATTCCCCAAATTTTTAAATAGACACTTCATAAAAAAATCCTCCTAAATTAGAATAGTTTAAATATATCATTTTTGTTCAGTAATATTAATATGTACACTATTTCCAAAATTTAAATTCATGTTGCCAAAAATCGATTATGTCTTTTGCGGGGCAAAAAAAGGGCAGATTATTTGAAAAAGGGCAAACGCTTGTGGAAAAGCTAAAAGGTTAAAAATGACAAAAACCTTGATACAACAGTGTTTTTGGACGCTCGTGTACGTTAGAGAATGACCGGTTTACCATCATACAAGGATGGGATTAACTTGTGTTAAAAAGCCTTTAATATCAGTTGTTACAAAGGATTTGTAGCGTCTTTAAAAATAAAAAAGGGCAGAAAAAGGGCAGATACCTTTTAGTACACAAGTTTTTCTAATTTTTGCTCTAACTCTCTGTCCATTTTCTCTGTTACATGTGTATACACCTTTATAGTCGTTTTTTCCAGATCTGGAATTAATGAGGCATTCTAACATTACAGAAACAATTCAAACGTACTCTCATTTATATACTGATAAAAAACATCAAGCTATGAGCATATTTGATTAAACGGTATCAAATTGGTATCAAATAACAATTAAGGAGTTTATAAAATGCGTAATAACAAGCCTAAAATAAGTATTCAAAACGACCCATGGGAAGTGAAATTTATATACATTTAAATTTCATGAGACAATAAACGTTGATTTAATGCGTTTTTTTGCCTTTTTTATTTTCCTTATTTTTTCTGTTTTACAACAAAATGGTATCAAAAATGGTATCATTTGTAGTTATTTTAGCTTCACATATTAAAACAACCACACTCCTAAATTAATAGGTGGTGTGGTTTTGTTGGTTGTGTGGGGATCGATAGATCTGGATCTGGATTTTAATGCGGATGTTGCGATTACTTCGCCAACTATTGCGATAACAAGAAAAAGCCAGCCTTTCATGATATATCTCCCAATTTGTGTAGGGCTTATTATGCACGCTTAAAAATAATAAAAGCAGACTTGACCTGATAGTTTGGCTGTGAGCAATTATGTGCTTAGTGCATCTAACGCTTGAGTTAAGCCGCGCCGCGAAGCGGCGTCGGCTTGAACGAATTGTTAGACATTATTTGCCGACTACCTTGGTGATCTCGCCTTTCACGTAGTGGACAAATTCTTCCAACTGATCTGCGCGCGAGGCCAAGCGATCTTCTTCTTGTCCAAGATAAGCCTGTCTAGCTTCAAGTATGACGGGCTGATACTGGGCCGGCAGGCGCTCCATTGCCCAGTCGGCAGCGACATCCTTCGGCGCGATTTTGCCGGTTACTGCGCTGTACCAAATGCGGGACAACGTAAGCACTACATTTCGCTCATCGCCAGCCCAGTCGGGCGGCGAGTTCCATAGCGTTAAGGTTTCATTTAGCGCCTCAAATAGATCCTGTTCAGGAACCGGATCAAAGAGTTCCTCCGCCGCTGGACCTACCAAGGCAACGCTATGTTCTCTTGCTTTTGTCAGCAAGATAGCCAGATCAATGTCGATCGTGGCTGGCTCGAAGATACCTGCAAGAATGTCATTGCGCTGCCATTCTCCAAATTGCAGTTCGCGCTTAGCTGGATAACGCCACGGAATGATGTCGTCGTGCACAACAATGGTGACTTCTACAGCGCGGAGAATCTCGCTCTCTCCAGGGGAAGCCGAAGTTTCCAAAAGGTCGTTGATCAAAGCTCGCCGCGTTGTTTCATCAAGCCTTACGGTCACCGTAACCAGCAAATCAATATCACTGTGTGGCTTCAGGCCGCCATCCACTGCGGAGCCGTACAAATGTACGGCCAGCAACGTCGGTTCGAGATGGCGCTCGATGACGCCAACTACCTCTGATAGTTGAGTCGATACTTCGGCGATCACCGCTTCCCTCATGATGTTTAACTTTGTTTTAGGGCGACTGCCCTGCTGCGTAACATCGTTGCTGCTCCATAACATCAAACATCGACCCACGGCGTAACGCGCTTGCTGCTTGGATGCCCGAGGCATAGACTGTACCCCAAAAAAACAGTCATAACAAGCCATGAAAACCGCCACTGCGCCGTTACCACCGCTGCGTTCGGTCAAGGTTCTGGACCAGTTGCGTGAGCGCATACGCTACTTGCATTACAGCTTACGAACCGAACAGGCTTATGTCCACTGGGTTCGTGCCTTCATCCGTTTCCACGGTGTGCGTCACCCGGCAACCTTGGGCAGCAGCGAAGTCGAGGCATTTCTGTCCTGGCTGGCGAACGAGCGCAAGGTTTCGGTCTCCACGCATCGTCAGGCATTGGCGGCCTTGCTGTTCTTCTACGGCAAGGTGCTGTGCACGGATCTGCCCTGGCTTCAGGAGATCGGAAGACCTCGGCCGTCGCGGCGCTTGCCGGTGGTGCTGACCCCGGATGAAGTGGTTCGCATCCTCGGTTTTCTGGAAGGCGAGCATCGTTTGTTCGCCCAGCTTCTGTATGGAACGGGCATGCGGATCAGTGAGGGTTTGCAACTGCGGGTCAAGGATCTGGATTTCGATCACGGCACGATCATCGTGCGGGAGGGCAAGGGCTCCAAGGATCGGGCCTTGATGTTACCCGAGAGCTTGGCACCCAGCCTGCGCGAGCAGGGGAATTAATTCCCACGGGTTTTGCTGCCCGCAAACGGGCTGTTCTGGTGTTGCTAGTTTGTTATCAGAATCGCAGATCCGGCTTCAGCCGGTTTGCCGGCTGAAAGCGCTATTTCTTCCAGAATTGCCATGATTTTTTCCCCACGGGAGGCGTCACTGGCTCCCGTGTTGTCGGCAGCTTTGATTCGATAAGCAGCATCGCCTGTTTCAGGCTGTCTATGTGTGACTGTTGAGCTGTAACAAGTTGTCTCAGGTGTTCAATTTCATGTTCTAGTTGCTTTGTTTTACTGGTTTCACCTGTTCTATTAGGTGTTACATGCTGTTCATCTGTTACATTGTCGATCTGTTCATGGTGAACAGCTTTGAATGCACCAAAAACTCGTAAAAGCTCTGATGTATCTATCTTTTTTACACCGTTTTCATCTGTGCATATGGACAGTTTTCCCTTTGATATGTAACGGTGAACAGTTGTTCTACTTTTGTTTGTTAGTCTTGATGCTTCACTGATAGATACAAGAGCCATAAGAACCTCAGATCCTTCCGTATTTAGCCAGTATGTTCTCTAGTGTGGTTCGTTGTTTTTGCGTGAGCCATGAGAACGAACCATTGAGATCATACTTACTTTGCATGTCACTCAAAAATTTTGCCTCAAAACTGGTGAGCTGAATTTTTGCAGTTAAAGCATCGTGTAGTGTTTTTCTTAGTCCGTTATGTAGGTAGGAATCTGATGTAATGGTTGTTGGTATTTTGTCACCATTCATTTTTATCTGGTTGTTCTCAAGTTCGGTTACGAGATCCATTTGTCTATCTAGTTCAACTTGGAAAATCAACGTATCAGTCGGGCGGCCTCGCTTATCAACCACCAATTTCATATTGCTGTAAGTGTTTAAATCTTTACTTATTGGTTTCAAAACCCATTGGTTAAGCCTTTTAAACTCATGGTAGTTATTTTCAAGCATTAACATGAACTTAAATTCATCAAGGCTAATCTCTATATTTGCCTTGTGAGTTTTCTTTTGTGTTAGTTCTTTTAATAACCACTCATAAATCCTCATAGAGTATTTGTTTTCAAAAGACTTAACATGTTCCAGATTATATTTTATGAATTTTTTTAACTGGAAAAGATAAGGCAATATCTCTTCACTAAAAACTAATTCTAATTTTTCGCTTGAGAACTTGGCATAGTTTGTCCACTGGAAAATCTCAAAGCCTTTAACCAAAGGATTCCTGATTTCCACAGTTCTCGTCATCAGCTCTCTGGTTGCTTTAGCTAATACACCATAAGCATTTTCCCTACTGATGTTCATCATCTGAGCGTATTGGTTATAAGTGAACGATACCGTCCGTTCTTTCCTTGTAGGGTTTTCAATCGTGGGGTTGAGTAGTGCCACACAGCATAAAATTAGCTTGGTTTCATGCTCCGTTAAGTCATAGCGACTAATCGCTAGTTCATTTGCTTTGAAAACAACTAATTCAGACATACATCTCAATTGGTCTAGGTGATTTTAATCACTATACCAATTGAGATGGGCTAGTCAATGATAATTACTAGTCCTTTTCCTTTGAGTTGTGGGTATCTGTAAATTCTGCTAGACCTTTGCTGGAAAACTTGTAAATTCTGCTAGACCCTCTGTAAATTCCGGTAGACCTTTGTGTGTTTTTTTTGTTTATATTCAAGTGGTTATAATTTATAGAATAAAGAAAGAATAAAAAAAGATAAAAAGAATAGATCCCAGCCCTGTGTATAACTCACTACTTTAGTCAGTTCCGCAGTATTACAAAAGGATGTCGCAAACGCTGTTTGCTCCTCTACAAAACAGACCTTAAAACCCTAAAGGCTTAAGTAGCACCCTCGCAAGCTCGGGCAAATCGCTGAATATTCCTTTTGTCTCCGACCATCAGGCACCTGAGTCGCTGTCTTTTTCGTGACATTCAGTTCGCTGCGCTCACGGCTCTGGCAGTGAATGGGGGTAAATGGCACTACAGGCGCCTTTTATGGATTCATGCAAGGAAACTACCCATAATACAAGAAAAGCCCGTCACGGGCTTCTCAGGGCGTTTTATGGCGGGTCTGCTATGTGGTGCTATCTGACTTTTTGCTGTTCAGCAGTTCCTGCCCTCTGATTTTCCAGTCTGACCACTTCGGATTATCCCGTGACAGGTCATTCAGACTGGCTAATGCACCCAGTAAGGCAGCGGTATCATCAACAGGCTTACCCGTCTTACTGTCGGGAATTCCGATCCCCAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTAATTCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGGGAATTGGGAATTGATCCGCTGTAATGGGCCCAATCACTAGTGAATTCCCGAAGCTTAGATCTAATC

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Product Documents

P10318_Full Sequence Map.dna

Plasmid Elements

                                                            
     MCS    1..57
                     /note="pUC18/19多克隆位点"
     primer_bind     complement(533..554)
     SmR             complement(3285..4076)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /note="赋予对壮观霉素和链霉素的抗性"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(5328..6278)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     pSC101 ori      6326..6548
                     /note="需要Rep101蛋白的低拷贝复制起点"
     primer_bind     6527..6547
     terminator      7126..7212
                     /gene="Escherichia coli rrnB"
                     /note="大肠杆菌rrnB基因的转录终止子T1"
     terminator      7309..7395
                     /gene="Escherichia coli rrnB"
                     /note="大肠杆菌rrnB基因的转录终止子T1"
     terminator      7492..7578
                     /gene="Escherichia coli rrnB"
                     /note="大肠杆菌rrnB基因的转录终止子T1"
     terminator      7675..7761
                     /gene="Escherichia coli rrnB"
                     /note="大肠杆菌rrnB基因的转录终止子T1"
     terminator      7858..7944
                     /gene="Escherichia coli rrnB"
                     /note="大肠杆菌rrnB基因的转录终止子T1"                             
                        