产品声明
For Research Use Only research use only . They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.Plasmid Sequence and Experimental Performance reference/template plasmid . MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.Sequence Verification of Certain Vector Products key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.After-Sales Period for Small-Pack Plasmid Products three months from the date of receipt . Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.Research-Related Risks and Limitation of Liability Citation in Chinese Publications 产品名称（产品货号）由淼灵质粒平台提供。 Citation in English Publications “Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”
质粒序列
一键复制序列（有部分质粒是非全长序列）
CTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCGATAAACCCAGCGAACCATTTGAGGTGATAGGTAAGATTATACCGAGGTATGAAAACGAGAATTGGACCTTTACAGAATTACTCTATGAAGCGCCATATTTAAAAAGCTACCAAGACGAAGAGGATGAAGAGGATGAGGAGGCAGATTGCCTTGAATATATTGACAATACTGATAAGATAATATATCTTTTATATAGAAGATCGATTTTCGTTCGTGAATACATGTTATAATAACTATAACTAATAACGTAACGTGACTGGCAAGAGATATTTTTAAAACAATGAATAGGTTTACACTTACTTTAGTTTTATGGAAATGAAAGATCATATCATATATAATCTAGAATAAAATTAACTAAAATAATTATTATCTAGATAAAAAATTTAGAAGCCAATGAAATCTATAAATAAACTAAATTAAGTTTATTTAATTAACAACTATGGATATAAAATAGGTACTAATCAAAATAGTGAGGAGGATATATTTGAATACATACGAACAAATTAATAAAGTGAAAAAAATACTTCGGAAACATTTAAAAAATAACCTTATTGGTACTTACATGTTTGGATCAGGAGTTGAGAGTGGACTAAAACCAAATAGTGATCTTGACTTTTTAGTCGTCGTATCTGAACCATTGACAGATCAAAGTAAAGAAATACTTATACAAAAAATTAGACCTATTTCAAAAAAAATAGGAGATAAAAGCAACTTACGATATATTGAATTAACAATTATTATTCAGCAAGAAATGGTACCGTGGAATCATCCTCCCAAACAAGAATTTATTTATGGAGAATGGTTACAAGAGCTTTATGAACAAGGATACATTCCTCAGAAGGAATTAAATTCAGATTTAACCATAATGCTTTACCAAGCAAAACGAAAAAATAAAAGAATATACGGAAATTATGACTTAGAGGAATTACTACCTGATATTCCATTTTCTGATGTGAGAAGAGCCATTATGGATTCGTCAGAGGAATTAATAGATAATTATCAGGATGATGAAACCAACTCTATATTAACTTTATGCCGTATGATTTTAACTATGGACACGGGTAAAATCATACCAAAAGATATTGCGGGAAATGCAGTGGCTGAATCTTCTCCATTAGAACATAGGGAGAGAATTTTGTTAGCAGTTCGTAGTTATCTTGGAGAGAATATTGAATGGACTAATGAAAATGTAAATTTAACTATAAACTATTTAAATAACAGATTAAAAAAATTATAAAAAAATTGAAAAAATGGTGGAAACACTTTTTTCAATTTTTTTGTTTTATTATTTAATATTTGGGAAATATTCATTCTAATTGGTAATCAGATTTTAGAAAACAATAAACCCTTGCATAACTTTCTCGTCCATATCGGAAACTACTTTTTTATTCCCTTTTTTTCGACCGAGATTATTTTGCGATAAAATCCGATTAAGATACTGCCTACTAACTCCCAATTCTTGAGCTAATTCTGATACTGTTTTACTCATCAGCTACCTCACAAAATAATTCTTTCTATCCCAATTCCAAAAGGCAACAATTTCACGACCAGTACTTTCTGCAGATTCTTCTGCTCCGGTCTGAACAAGATTCCCTTCTTCGACATCATCAAGAGCTAACTCCGCCTTGATTTTCTTGAACAACTTTCCAAAACCAATCTGCCTTTTTCGGTGCAAACCATTCGTTAAATCTTCTGTTATCCGTTCTTTGACCATTTCAGAGAATAATGTCACATCTTCTGTATCTACATCAAAAGGCTTAACAGGATATTTAGCTGTTTCGATAATAGCTGACTTCAAGTTTTTACGTTTATGAGCTTTGACGGTTCGAATATCAACTACAGGTGTATAATCCAATTTCATCGCCTTAGCCCATAGTTTCGTCCATTCTTCTTGGCTAATGTAATTTGTATTATTTCCAGTAAAATAGCTAGATTTCACAAATAACAATACATGCAAATGAGGGTGATAATTCTCATGCTCAGTTGAATAAGTTACCTCAGTGGCTCGCAAATAGCCAATAACATTTTTATCGACTTTCTTGTATTTCATAAGACGATTAAAAGCTCGCCCTATTTCTGAAATTGATTTATTTAATTTTTCACCACTAATGTTCTTAATCGTCAAGGTCAAAAAGAGAAAGCGACCTTTTGGATAGCGTTTCATTGCTTCATTTACCACTAATTCAGCTTGATAAGCATACTTCATTGACCGTCTCCAGTTACAAATTGGGCAGAGCTTATTTTTGCAAAAATAAGACTGATAGAGTTTTTTCGTGCCGTCTTGTTGTTCGATAAACTTCAACACTTCGGCACATTGATAGACCCGTTCAAACGAACGATATCCTAAACGCTCTAAACGACCAGCAAGCTCAATATTTTTTAACTTGCGTTCTCGCCACTTTCTATCTTTGCCATTCTTAGAATAGTCCTTAAAGACTTGATTTTCAGTACCTGTCATGTTATAATTTTCTTGCAAAAAAATTTTTTTGAGTATATAAAAAAGTTCCTGCAATTCTCCTAAGTTTATTATTTTAAATTGGACACTTAAATTATAAACTTTATTTTATTAAATTGCAAGGGCTTTTTTGTTACCTAAAAACCCAGCAATATCAAGGGTTTGAGGTGCTTTAAAACAAGAAAATAAAAAAACTCATTATTTCTATATGTATCAAGATAAGAAAAAACCCTTGCTACGCAATTGGGTTTTTAAGGGCTTTTCAGCCCTCTTTTTCGTTTCACGAAAAATTCACCCCTAAAACCTCAAATTTTGACTTTCTAAATCCAAACGTGGTATAATAATTTTAACGCTAGAAGTTCTAGGAAACGAATAGCACAACGCAAAAAAAACAGTAGCTGGAACTACTGTCTTTCTTTTTTGTCCTCAAAAAGAACTCAGGTTAATTTTTGTCGCGTTTGTTTCGAACATACCGAATCAACCAATCAGCACTTACCTCTGCAGCAACGCCAACCACAAAAGGTATCAAAACAAGTTCTAGGAAAGTCTTCACAGCGCTCACCTCCAATCTAAGTTCGTAACTTAGCGAAAGAACGTTGCGACCTAGCTATTATACCATGTCCGCTTACTTTATTCCATGGTCTAATTGACAACAAGTAACCAAGGGCGGACGTCCTTTGTCCGTGTCGGCTCGAAACGCTAAAGCCTTTCGGCTCGTCACGTCCTAGCGTACTTTGCCCTTGCTTATTGTCAATTAGCTTTCACGGCATAAATCGCTCAAAGGCCTAGCCCTTTTTCAATCACTCGTTTAACTACCCTTACGATTGGCTGAATAGCTCTTAACTCTGATATAAAATTTTTTAAGGCTTTATCTGGCATAAACTCTTTCAGTTTTCCTAGCATTTTGTCATTTTGCTGTCTCAAAACTGAGTTTTCACTTCTAAGCGCTCTATTTTCGTTCCTAAGACGCTCTATGCTCTCTCCCTTACTTTTTTCTTTAGCTCGTTCCAACTCGCTTCAAACCGTTTAGAGCGTCTCAGAGGCACTATCCGCCTTAATTGCTCATTCTCTAGCTCTTTTTGATGAACAAGGTTTCTGCTTCGGTATATCAGCCCTTTAAATTCTTCAAACTGCTCTTTAGTCAATTCCACATATTCTTTACCGAACACGCCTTTTTTAGGGGTAATAGCCGCTAGTTCTTTCTCAGACATGATTTTTAGGTCAGCTATTTGTGTTTTTAGTTTTTCTAATTTCAGCCCCTCGCGCTCTAAACGCTCTATTTTCGCTTCTAAGCTCTTCGAATAATTTTCTAAGGTATTTATATATCCCTCAGCTTTAGATAGCTCTGAGAGCCTCTCAGAGGTCTCAGAATCGCCAGGACCCAACGCTGCCCGACTGCTTTCCTGATGCAAAAACGAGGCTAGTTTACCGTATCTGTGGGGGGATGGCTTGTAGATATGACGACAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAATTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACAGCTGCTTTCCTGATGCAAAAACGAGGCTAGTTTACCGTATCTGTGGGGGGATGGCTTGTAGATATGACGACAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAATTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACAGCCGAGATGCGCCGCGTGCGGCTGCTGGAGATGGCGGACGCGATGGATATGTTCTGCCAAGGGTTGGTTTGCGCATTCACAGTTCTCCGCAAGAATTGATTGGCTCCAATTCTTGGAGTGGTGAATAATTCCCGGCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGAGCTTGTTGTAACTGAAAAAGGAAAATTATTGTGCCAGGCAGTTGAAAGTCAGCACCTTTTAACGAGTGCTGAAATGACGGCTAAATGGGAAACGTATTT
质粒元件
ori complement(182..770)
/direction=LEFT
/note="高拷贝数ColE1/pMB1/pBR322/pUC复制起点"
terminator 5100..5127
/note="大肠杆菌rrnB基因的转录终止子T2"
terminator 5219..5305
/gene="Escherichia coli rrnB"
/note="大肠杆菌rrnB基因的转录终止子T1"
terminator 5598..5625
/note="大肠杆菌rrnB基因的转录终止子T2"
terminator 5717..5803
/gene="Escherichia coli rrnB"
/note="大肠杆菌rrnB基因的转录终止子T1"
lacZ-alpha complement(5969..6367)
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
LAAHPPFASWRNSEEARTDRPSQQLRSLNGECRELFTTPRIGANQFLRRTVNAQTNPWQ
NISIASAISSSRTRRISAVLADERRFSA"
primer_bind 6277..6293
/note="常见的测序引物，多种相似变体之一"
MCS 6294..6350
/note="pUC18/19多克隆位点"
primer_bind complement(6363..6379)
/note="常见的测序引物，多种相似变体之一"
lac operator 6387..6403
/bound_moiety="lac repressor encoded by lacI"
/note="lac阻遏物与lac操作员结合，抑制大肠杆菌中的转录。这种抑制可以通过添加乳糖或异丙基β-D-硫代吡喃半乳糖苷（IPTG）来缓解。"
lac promoter complement(6411..6441)
/note="大肠杆菌lac操纵子的启动子"
CAP binding site 6456..6477
/bound_moiety="E. coli catabolite activator protein"
/note="CAP结合在cAMP存在下激活转录。"
