产品声明
For Research Use Only research use only . They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.Plasmid Sequence and Experimental Performance reference/template plasmid . MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.Sequence Verification of Certain Vector Products key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.After-Sales Period for Small-Pack Plasmid Products three months from the date of receipt . Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.Research-Related Risks and Limitation of Liability Citation in Chinese Publications 产品名称（产品货号）由淼灵质粒平台提供。 Citation in English Publications “Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”
质粒序列
一键复制序列（有部分质粒是非全长序列）
TGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATTCGACGCTCTCCCTTATGCGACTCCTGCATTAGGAAGCAGCCCAGTAGTAGGTTGAGGCCGTTGAGCACCGCCGCCGCAAGGAATGGTGCATGCAAGGAGATGGCGCCCAACAGTCCCCCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCTCATGAGCCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGGCGCCAGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCGGCGTAGAGGATCTGGCTAGCGATGACCCTGCTGATTGGTTCGCTGACCATTTCCGGGTGCGGGACGGCGTTACCAGAAACTCAGAAGGTTCGTCCAACCAAACCGACTCTGACGGCAGTTTACGAGAGAGATGATAGGGTCTGCTTCAGTAAGCCAGATGCTACACAATTAGGCTTGTACATATTGTCGTTAGAACGCGGCTACAATTAATACATAACCTTATGTATCATACACATACGATTTAGGTGACACTATAGAATACACGGAATTAATTCTAGACTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAATTCTCATGTTTGACAGCTTATCATCGCCACTCCAGCCAGCCCCACTCATGTTCCCCCCGCACCACATATGCACATGCACGCATGTACACACAACTTGTAGTGCTCTTTCAAAGGTTTTATTCATTGCAGAGGGGTGGACAGGGTAGTGGTGGGCCTAGTTGCAGTAGTTCTCCAGTTGGTAGAGAGAGCAGATGCTGGTGCAGCACTGATCCACGATGCCGCGCTTCTGCCGGGCCACCTCCAGTGCCAAGGTCTGAAGGTCACCGGGGCGGCCGCTCAGCAGTTCTTGAAGTACTCCGGATGCAGCTCTCGGGCCATGTGATGAAATGCTAGGCGGCTGTCAAACCTCCACTCTAATACTTCTCTCTCCGGGTCATCCATCCCATGCAGGCTCACAGGGTGTAACAAGCGGGTGTTCTCTCCTTCATTGGCTTCTTCTACCTTCTCTTGCTCAACTGGTACTAGCTTGTAGCACCATCCAAAGGTCAGTGGATATCTGATCCCTGGCCCTGGTGTGTAGTTCTGCCAATCAGGGAAGTAGCCTTGTGTGTGGTAGATCCACAGATCAAGGATATCTTGTCTTCGTTGGGAGTGAATTAGCCCTTCCAGTCCCCCCTTTTCTTTTAAAAAGTGGCTAAGATCTACAGCTGCCTTGTAAGTCATTGGTCTTAAAGGTACCTGAGGTGTGACTGGAAAACCCACCTCCTCCTCCTCTTGTGCTTCTAGCCAGGCACAATCAGCATTGGTAGCTGCTGTATTGCTACTTGTGATTGCTCCATGTTTTTCTAGGTCTCGAGATACTGCTCCCACCCCATCTGCTGCTGGCTCAGCTCGTCTCATTCTTTCCCTTACAGTAGGCCATCCAATCACACTACTTTTTGACCACTTGCCACCCATCTTATAGCAAAATCCTTTCCAAGCCCTGTCTTATTCTTCTAGGTATGTGGCGAATAGCTCTACAAGCTCCTTGTACTACTTCTATAACCCTATCTGTCCCCTCAGCTACTGCTATGGCTGTGGCATTGAGCAAGCTAACAGCACTATTCTTTAGCTCCTGACTCCAATATTGTAGGAGATTCCACCAATATTTGAGGGCTTCCCACCCCCTGCGTCCCAGAAGTTCCACAATCCTCGTTACAATCAAGAGTAAGTCTCTCAAGCGGTGGTAGCTGAAGAGGCACAGGCTCCGCAGATCGTCCCAGATAAGTGCCAAGGATCCGTTCACTAATCGAATGGATCTGTCTCTGTCTCTCTCTCCACCTTCTTCTTCTATTCCTTCGGGCCTGTCGGGTCCCCTCGGGGTTGGGAGGTGGGTCTGAAACGATAATGGTGAATATCCCTGCCTAACTCTATTCACTATAGAAAGTACAGCAAAAACTATTCTTAAACCTACCAAGCCTCCTACTATCATTATGAATAATTTTATATACCACAGCCAATTTGTTATGTTAAACCAATTCCACAAACTTGCCCATTTATCTAATTCCAATAATTCTTGTTCATTCTTTTCTTGCTGGTTTTGCGATTCTTCAATTAAGGAGTGTATTAAGCTTGTGTAATTGTTAATTTCTCTGTCCCACTCCATCCAGGTCGTGTGATTCCAAATCTGTTCCAGAGATTTATTACTCCAACTAGCATTCCAAGGCACAGCAGTGGTGCAAATGAGTTTTCCAGAGCAACCCCAAATCCCCAGGAGCTGTTGATCCTTTAGGTATCTTTCCACAGCCAGGATTCTTGCCTGGAGCTGCTTGATGCCCCAGACTGTGAGTTGCAACAGATGCTGTTGCGCCTCAATAGCCCTCAGCAAATTGTTCTGCTGCTGCACTATACCAGACAATAATTGTCTGGCCTGTACCGTCAGCGTCATTGACGCTGCGCCCATAGTGCTTCCTGCTGCTCCCAAGAACCCAAGGAACAAAGCTCCTATTCCCACTGCTCTTTTTTCTCTCTGCACCACTCTTCTCTTTGCCTTGGTGGGTGCTACTCCTAATGGTTCAATTTTTACTACTTTATATTTATATAATTCACTTCTCCAATTGTCCCTCATATCTCCTCCTCCAGGTCTGAAGATCAGCGGCCGCTCAACTACAGATCATCAATATCCCAAGGAGCATGGTGCCCCATTTCCACCCCCATCTCCACAAGTGCTGATACTTCTCCTTCACTCTCATTGCCACTGTCTTCTGCTCTTTCTATTAGTCTATCAATTAACCTGTCTATTTTTCTTTGTCTTAATATTTTCCTATATTCTATGATTACTATGGACCACACAACTATTGCTATTATTATTGCTACTACTAATGCTACTATTGCTACTATTATAGGTTGCATTACATGTACTACTTACTGCTTTGATAGAGAAGCTTGATGAGTCTGACTGTTCTGATGAGCTCTTCGTCGCTGTCTCCGCTTCTTCCTGCCATAGGAGATGCCTAAGGCTTTTGTCATGAAACAAACTTGGCAATGAAAGCAACACTTTTTACAATAGCAATTGGTACAAGCAGTTTTAGGCTGACTTCCTGGATGCTTCCAGGGCTCTAGTCTAGGATCTACTGGCTCCATTTCTTGCTCTCCTCTGTCGAGTAACGCCTATTCTGCTATGTCGACACCCAATTCTGAAATGGATAAACAGCAGTTGTTGCAGAATTCTTATTATGGCTTCCACTTCGGTCCGCCCCGGGTTCGGGCTACTAAAGCTCTAGTGTCCATTCATTGTATGGCTCCCTCTGTGGCCCTTGGTCTTCTGGGGCTTGTTCCATCTGTCCTCTGTCAGTTTCCTAACACTAGGCAAAGGTGGCTTTATCTGTTTTGGTTTTATTAATGCTGCTAGTGCCAAGTACTGTAGAGATCCTACCTTGTTATGTCCTGCTTGATATTCACACCTAGGACTAACTATACGTCCTAATATGGTATTTCTTATAGCAGATTCTGAAAAACAATCAAAATAGTGCAGATGAATTAGTTGGTCTGCTAGGTCAGGGTCTACTTGTGTGCTATATCTCTTTTTCCTCCATTCTATGGAGACTCCCTGACCCAAATGCCAGTCTCTTTCTCCTGTATGCAGACCCCAATATGTTGTTATTACTAATTTAGCATCCCCTAGTGGGATGTGTACTTCTGAACTTATTTTTGGATTAGTACTTTCATAGTGATGTCTATAAAACCAGTCCTTAGCTTTCCTTGAAATATACATATGGTGTTTTACTAATCTTTTCCATGTGTTAATCCTCATCCTGTCTACTTGCCACACAATCATCACCTGCCATCTGTTTTCCATAATCCCTGATGATCTTTGCTTTTCTTCTTGGCACTACTTTTATGTCACTATTATCTTGTATTACTACTGCCCCTTCACCTTTCCAGAGGAGCTTTGCTGGTCCTTTCCAAACTGGATCTCTGCTGTCCCTGTAATAAACCCGAAAATTTTGAATTTTTGTAATTTGTTTTTGTAATTCTTTAGTTTGTATGTCTGTTGCTATTATGTCTACTATTCTTTCCCCTGCACTGTACCCCCCAATCCCCCCTTTTCTTTTAAAATTGTGGATGAATACTGCCATTTGTACTGCTGTCTTAAGATGTTCAGCCTGATCTCTTACCTGTCCTATAATTTTCTTTAATTCTTTATTCATAGATTCTATTACTCCTTGACTTTGGGGATTGTAGGGAATGCCAAATTCCTGCTTGATCCCCGCCCACCAACAGGCGGCCTTAACTGTAGTACTGGTGAAATTGCTGCCATTGTCTGTATGTACTGTTTTTACTGGCCATCTTCCTGCTAATTTTAAGAGGAAGTATGCTGTTTCTTGCCCTGTCTCTGCTGGAATTACTTCTGCTTCTATATATCCACTGGCTACATGAACTGCTACCAAGATAACTTTTCCTTCTAAATGTGTACAATCTAGCTGCCATATTCCTGGGCTACAGTCTACTTGTCCATGCATGGCTTCCCCTTTTAGCTGACATTTATCACAGCTGGCTACTATTTCTTTTGCTACTACAGGTGGTAGGTTAAAATCACTAGCCATTGCTCTCCAATTACTGTGATATTTCTCATGTTCTTCTTGGGCCTTATCTATTCCATCTAAAAATAGTACTTTCCTGATTCCAGCACTGACCAATTTATCTACTTGTTCATTTCCTCCAATTCCTTTGTGTGCTGGTACCCATGCCAGGTAGACTTTTTCCTTTTTTATTAACTGCTCTATTATTTGACTGACTAACTCTGATTCACTCTTATCTGGTTGTGCTTGAATGATTCCCAATGCATATTGTGAGTCTGTCACTATGTTTACTTCTAATCCCGAATCCTGCAAAGCTAGATGAATTGCTTGTAACTCAGTCTTCTGATTTGTTGTGTCCGTTAGGGGGACAACTTTTTGTCTTCCTCTGTCAGTTACATATCCTGCTTTTCCTAATTTAGTTTCCCTATTGGCTGCCCCATCTACATAGAAAGTTTCTGCTCCTATTATGGGTTCTTTCTCTAACTGGTACCATAACTTCACTAAGGGAGGGGTATTGACAAACTCCCACTCAGGAATCCAGGTGGCTTGCCAATACTCTGTCCACCATGCTTCCCATGTTTCCTTTTGTATGGGTAATTTAAATTTAGGAGTCTTTCCCCATATTACTATGCTTTCTGTGGCTATTTTTTGTACTGCCTCTGTTAATTGTTTCACATCATTAGTGTGGGCACCCTTCATTCTTGCATACTTTCCTGTTTTCAGATTTTTAAATGGCTCTTGATAAATTTGATATGTCCATTGGCCTTGCCCCTGCTTCTGTATTTCTGCTATTAAGTCTTTTGATGGGTCATAATACACTCCATGTACCGGTTCTTTTAGAATCTCCCTGTTTTCTGCCAGTTCTAGCTCTGCTTCTTCTGTTAGTGGTACTACTTCTGTTAGTGCTTTGGTTCCCCTAAGAAGTTTACATAATTGCCTTACTTTAATCCCTGCATAAATCTGACTTGCCCAATTCAATTTTCCCACTAATTTCTGTATGTCATTGACAGTCCAGCTGTCCTTTTCTGGCAGCACTATAGGCTGTACTGTCCATTTATCAGGATGGAGTTCATAACCCATCCAAAGGAATGGAGGTTCTTTCTGATGTTTTTTGTCTGGTGTGGTAAATCCCCACCTCAACAGATGTTGTCTCAGTTCCTCTATTTTTGTTCTATGCTGCCCTATTTCTAAGTCAGATCCTACATACAAATCATCCATGTATTGATAGATGACTATGTCTGGATTTTGTTTTCTAAAAGGCTCTAAGATTTTTGTCATGCTACACTGGAATATTGCTGGTGATCCTTTCCATCCCTGTGGAAGCACATTGTACTGATATCTAATCCCTGGTGTCTCATTGTTTATACTAGGTATGGTAAATGCAGTATACTTCCTGAAGTCTTTATCTAAGGGAACTGAAAAATATGCATCGCCCACATCCAGTACTGTTACTGATTTTTTCTGTTTTAACCCTGCAGGATGTGGTATTCCTAATTGAACTTCCCAGAAATCTTGAGTTCTCTTATTAAGTTCTCTGAAATCTACTAATTTTCTCCATTTAGTACTGTCTTTTTTCTTTATGGCAAATACTGGAGTATTGTATGGATTTTCAGGCCCAATTTTTGAAATTTTTCCTTCCTTTTCCATTTCTGTACAAATTTCTACTAATGCTTTTATTTTTTCTTCTGTCAATGGCCATTGTTTAACTTTTGGGCCATCCATTCCTGGCTTTAATTTTACTGGTACAGTCTCAATAGGACTAATGGGAAAATTTAAAGTGCAGCCAATCTGAGTCAACAGATTTCTTCCAATTATGTTGACAGGTGTAGGTCCTACTAATACTGTACCTATAGCTTTATGTCCGCAGATTTCTATGAGTATCTGATCATACTGTCTTACTTTGATAAAACCTCCAATTCCCCCTATCATTTTTGGTTTCCATCTTCCTGGCAAATTCATTTCTTCTAATACTGTATCATCTGCTCCTGTATCTAATAGAGCTTCCTTTAATTGCCCCCCTATCTTTATTGTGACGAGGGGTCGCTGCCAAAGAGTGATCTGAGGGAAGCTAAAGGATACAGTTCCTTGTCTATCGGCTCCTGCTTCTGAGAGGGAGTTGTTGTCTCTTCCCCAAACCTGAAGCTCTCTTCTGGTGGGGCTGTTGGCTCTGGTCTGCTCTGAAGAAAATTCCCTGGCCTTCCCTTGTGGGAAGGCCAGATCTTCCCTAAAAAATTAGCCTGTCTCTCAGTACAATCTTTCATTTGGTGTCCTTCCTTTCCACATTTCCAACAGCCCTTTTTCCTAGGGGCCCTGCAATTTTTGGCTATGTGCCCTTCTTTGCCACAATTGAAACACTTAACAGTCTTTCTTTGGTTCCTAAAATTGCCTTTCTGTATCATTATGGTAGCTGGATTTGTTACTTGGCTCATTGCTTCAGCCAAAACTCTTGCTTTATGGCCGGGTCCCCCCACTCCCTGACATGCTGTCATCATTTCTTCTAGTGTCGCTCCTGGTCCCAATGCTTTTAAAATAGTCTTACAATCTGGGTTCGCATTTTGGACCAACAAGGTTTCTGTCATCCAATTTTTTACCTCTTGTGAAGCTTGCTCGGCTCTTAGAGTTTTATAGAATCGGTCTACATAGTCTCTAAAGGGTTCCTTTGGTCCTTGTCTTATGTCCAGAATGCTGGTAGGGCTATACATTCTTACTATTTTATTTAATCCCAGGATTATCCATCTTTTATAGATTTCTCCTACTGGGATAGGTGGATTATGTGTCATCCATCCTATTTGTTCCTGAAGGGTACTAGTAGTTCCTGCTATGTCACTTCCCCTTGGTTCTCTCATCTGGCCTGGTGCAATAGGCCCTGCATGCACTGGATGCACTCTATCCCATTCTGCAGCTTCCTCATTGATGGTCTCTTTTAACATTTGCATGGCTGCTTGATGTCCCCCCACTGTGTTTAGCATGGTGTTTAAATCTTGTGGGGTGGCTCCTTCTGATAATGCTGAAAACATGGGTATCACTTCTGGGCTGAAAGCCTTCTCTTCTACTACTTTTACCCATGCATTTAAAGTTCTAGGTGATATGGCCTGATGTACCATTTGCCCCTGGATGTTCTGCACTATAGGGTAATTTTGGCTGACCTGATTGCTGTGTCCTGTGTCAGCTGCTGCTTGCTGTGCTTTTTTCTTACTTTTGTTTTGCTCTTCCTCTATCTTGTCTAAAGCTTCCTTGGTGTCTTTTATCTCTATCCTTTGATGCACACAATAGAGGGTTGCTACTGTATTATATAATGATCTAAGTTCTTCTGATCCTGTCTGAAGGGATGGTTGTAGCTGTCCCAGTATTTGTCTACAGCCTTCTGATGTTTCTAACAGGCCAGGATTAACTGCGAATCGTTCTAGCTCCCTGCTTGCCCATACTATATGTTTTAATTTATATTTTTTCTTTCCCCCTGGCCTTAACCGAATTTTTTCCCATCGATCTAATTCTCCCCCGCTTAATACTGACGCTCTCGCACCCATCTCTCACCAGTCGCCGCCCCTCGCCTCTTGCCGTGCGCGTCAACGCGGCCGCGGACCTCGAAGCTTATTACGGGATCGATCCGTCGCATAAGAAGCCAAGGGGGTGGGCCTATAGACTCTATAGGCGGTACTTACGTCACTCTTGGCACGGGGAATCCGCGTTCCAATGCACCGTTCCCGGCCGCGGAGGCTGGATCGGTCCCGGTGTCTTCTATGGAGGTCAAAACAGCGTGGATGGCGTCTCCAGGCGATCTGACGGTTCACTAAACGAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCATTTGCGTCAATGGGGCGGAGTTGTTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGTGGAGACTTGGAAATCCCCGTGAGTCAAACCGCTATCCACGCCCATTGATGTACTGCCAAAACCGCATCACCATGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCCCATAAGGTCATGTACTGGGCATAATGCCAGGCGGGCCATTTACCGTCATTGACGTCAATAGGGGGCGTACTTGGCATATGATACACTTGATGTACTGCCAAGTGGGCAGTTTACCGTAAATACTCCACCCATTGACGTCAATGGAAAGTCCCTATTGGCGTTACTATGGGAACATACGTCATTATTGACGTCAATGGGCGGGGGTCGTTGGGCGGTCAGCCAGGCGGGCCATTTACCGTAAGTTATGTAACGCGGAACTCCATATATGGGCTATGAACTAATGACCCCGTAATTGATTACTATTAATAACTAGTCAATAATCAATGTCGGGCGAGCTCGAATTCCTGCAGCCCAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTATGGCTGATTATGATCTCTAGTCAAGGCACTATACATCAAATATTCCTTATTAACCCCTTTACAAATTAAAAAGCTAAAGGTACACAATTTTTGAGCATAGTTATTAATAGCAGACACTCTATGCCTGTGTGGAGTAAGAAAAAACAGTATGTTATGATTATAACTGTTATGCCTACTTATAAAGGTTACAGAATATTTTTCCATAATTTTCTTGTATAGCAGTGCAGCTTTTTCCTTTGTGGTGTAAATAGCAAAGCAAGCAAGAGTTCTATTACTAAACACAGCATGACTCAAAAAACTTAGCAATTCTGAAGGAAAGTCCTTGGGGTCTTCTACCTTTCTCTTCTTTTTTGGAGGAGTAGAATGTTGAGAGTCAGCAGTAGCCTCATCATCACTAGATGGCATTTCTTCTGAGCAAAACAGGTTTTCCTCATTAAAGGCATTCCACCACTGCTCCCATTCATCAGTTCCATAGGTTGGAATCTAAAATACACAAACAATTAGAATCAGTAGTTTAACACATTATACACTTAAAAATTTTATATTTACCTTAGAGCTTTAAATCTCTGTAGGTAGTTTGTCCAATTATGTCACACCACAGAAGTAAGGTTCCTTCACAAAGATCCGGGGCCCACTCATAAATCCAGTTGCCGCCACGGTAGCCAATCACCGTATCGTATAAATCATCGTCGGTACGTTCGGCATCGCTCATCACAATACGTGCCTGGACGTCGAGGATTTCGCGTGGGTCAATGCCGCGCCAGATCCACATCAGACGGTTAATCATGCGATACCAGTGAGGGATGGTTTTACCATCAAGGGCCGACTGCACAGGCGGTTGTGCGCCGTGATTAAAGCGGCGGACTAGCGTCGAGGTTTCAGGATGTTTAAAGCGGGGTTTGAACAGGGTTTCGCTCAGGTTTGCCTGTGTCATGGATGCAGCCTCCAGAATACTTACTGGAAACTATTGTAACCCGCCTGAAGTTAAAAAGAACAACGCCCGGCAGTGCCAGGCGTTGAAAAGATTAGCGACCGGAGATTGGCGGGACGAATACGACGCCCATATCCCACGGCTGTTCAATCCAGGTATCTTGCGGGATATCAACAACATAGTCATCAACCAGCGGACGACCAGCCGGTTTTGCGAAGATGGTGACAAAGTGCGCTTTTGGATACATTTCACGAATCGCAACCGCAGTACCACCGGTATCCACCAGGTCATCAATAACGATGAAGCCTTCGCCATCGCCTTCTGCGCGTTTCAGCACTTTAAGCTCGCGCTGGTTGTCGTGATCGTAGCTGGAAATACAAACGGTATCGACATGACGAATACCCAGTTCACGCGCCAGTAACGCACCCGGTACAGACCGCCACGGCTTACGGCAATAATGCCTTTCCATTGTTCAGAAGGCATCAGTCGGCTTGCGAGTTTACGTGCATGGATCTGCAACATGTCCCAGGTGACGATGTATTTTTCGCTCATGTGAAGTGTCCCAGCCTGTTTATCTACGGCTTAAAAAGTGTTCGAGGGGAAAATAGGTTGCGCGAGATTATAGAGATCTGGCGCACTAAAAACCAGTATTTCACATGAGTCCGCGTCTTTTTACGCACTGCCTCTCCCTGACGCGGGATAAAGTGGTATTCTCAAACATATCTCGCAAGCCTGTCTTGTGTCCAAGCTTTTTGCAAAAGCCTAGGCCTCCAAAAAAGCCTCCTCACTACTTCTGGAATAGCTCAGAGGCCGAGGCGGCCTCGGCCTCTGCATAAATAAAAAAAATTAGTCAGCCATGGGGCGGAGAATGGGCGGAACTGGGCGGAGTTAGGGGCGGGATGGGCGGAGTTAGGGGCGGGACTATGGTTGCTGACTAATTGAGATGCATGCTTTGCATACTTCTGCCTGCTGGGGAGCCTGGGGACTTTCCACACCTGGTTGCTGACTAATTGAGATGCATGCTTTGCATACTTCTGCCTGCTGGGGAGCCTGGGGACTTTCCACACCCTAACTGACACACATTCCACAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTT
质粒元件
ori complement(197..785)
/direction=LEFT
/note="高拷贝数ColE1/pMB1/pBR322/pUC复制起点"
AmpR complement(956..1816)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="对氨苄青霉素、羧苄青霉素和相关抗生素产生耐药性"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
AmpR promoter complement(1817..1921)
/gene="bla"
SP6 promoter 2709..2727
/note="噬菌体SP6 RNA聚合酶启动子"
NES complement(3964..3990)
/codon_start=1
/product="nuclear export signal from the HIV Rev protein
(Fischer et al., 1995)"
/translation="LPPLERLTL"
RRE 4513..4746
/note="HIV-1的Rev反应元件（RRE）允许Rev依赖性mRNA从细胞核输出到细胞质。"
HIV-1 pol complement(6043..9054)
/codon_start=1
/gene="pol"
/product="pol protein from human immunodeficiency virus 1"
/translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
MAGDDCVASRQDED"
cPPT/CTS 6241..6358
/note="HIV-1的中央多泌尿道和中央终止序列"
HIV-1 gag complement(8847..10349)
/codon_start=1
/gene="gag"
/product="gag protein from human immunodeficiency virus 1"
/translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFA
VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKI
EEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVEEKA
FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGP
IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
CMV promoter complement(10620..10823)
/note="人巨细胞病毒（CMV）即刻早期启动子"
CMV enhancer 10824..11203
/note="人类巨细胞病毒即刻早期增强子"
SV40 NLS complement(11653..11673)
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/translation="PKKKRKV"
intron 11803..11868
/note="SV40（猿猴病毒40）小t抗原内含子"
SV40 promoter complement(13002..13331)
/note="SV40增强子和早期启动子"
SV40 ori 13016..13151
/note="SV40复制起点"
