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pLVX-aMHC-eGFP-Rex-Neo

Catalog Number P1015
Alternative Names 等效质粒Plasmid#21229
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Product Overview

Product Disclaimer

  1. For Research Use Only
    All products available through the MiaoLing Plasmid Platform are intended for research use only. They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.
  2. Plasmid Sequence and Experimental Performance
    Plasmid products are provided and sold based on the corresponding reference/template plasmid. MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.
  3. Sequence Verification of Certain Vector Products
    For certain vector products, even the original manufacturer may not have fully verified the complete plasmid sequence. If the sequence of the key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.
  4. After-Sales Period for Small-Pack Plasmid Products
    The after-sales service period for small-pack plasmid products is three months from the date of receipt. Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.
  5. Research-Related Risks and Limitation of Liability
    Scientific research is inherently exploratory and involves a significant degree of uncertainty. To the maximum extent permitted by applicable law, MiaoLing shall not be liable for any additional economic losses, indirect losses, or other liabilities arising from the use of our products or from research outcomes.
  6. Citation in Chinese Publications
    For publications in Chinese, please cite the product as follows:
    Product names and IDs are provided by the MiaoLing Plasmid Platform.
  7. Citation in English Publications
    For publications in English, please cite the product as follows:
    “Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”

Related Publications

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Plasmid Elements

AmpR promoter 27..131 /gene="bla" AmpR 132..992 /codon_start=1 /gene="bla" /product="beta-lactamase" /note="对氨苄青霉素、羧苄青霉素和相关抗生素产生耐药性" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" ori 1163..1751 /direction=RIGHT /note="高拷贝数ColE1/pMB1/pBR322/pUC复制起点" CAP binding site 2039..2060 /bound_moiety="E. coli catabolite activator protein" /note="CAP结合在cAMP存在下激活转录。" lac promoter 2075..2105 /note="大肠杆菌lac操纵子的启动子" lac operator 2113..2129 /bound_moiety="lac repressor encoded by lacI" /note="lac阻遏物与lac操作员结合,抑制大肠杆菌中的转录。这种抑制可以通过添加乳糖或异丙基β-D-硫代吡喃半乳糖苷(IPTG)来缓解。" primer_bind 2137..2153 /note="常见的测序引物,多种相似变体之一" T3 promoter 2174..2192 /note="噬菌体T3 RNA聚合酶启动子" 5' LTR (truncated) 2448..2628 /note="HIV-1截短5'长末端重复序列(LTR)" HIV-1 Psi 2672..2797 /note="人类免疫缺陷病毒1型包装信号" RRE 3290..3523 /note="HIV-1的Rev反应元件(RRE)允许Rev依赖性mRNA从细胞核输出到细胞质。" cPPT/CTS 4019..4135 /note="HIV-1的中央多泌尿道和中央终止序列" aMHC promoter 4142..9656 primer_bind 4181..4197 /note="常见的测序引物,多种相似变体之一" EGFP 9670..10389 /codon_start=1 /product="enhanced GFP" /note="哺乳动物密码子优化" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" WPRE 10405..10993 /note="土拨鼠肝炎病毒转录后调控元件" Factor Xa site complement(10876..10887) /codon_start=1 /product="Factor Xa recognition and cleavage site" Cleavage site after base 10875" /translation="IEGR" NeoR/KanR 11735..12538 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="赋予对新霉素、卡那霉素和G418(遗传霉素(R))的抗性" /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" 3' LTR (Delta-U3) 12611..12844 /note="HIV-1自失活3'长末端重复序列" SV40 poly(A) signal 12916..13037 /note="SV40聚腺苷酸化信号" SV40 ori 13056..13191 /note="SV40复制起点" T7 promoter complement(13224..13242) /note="噬菌体T7 RNA聚合酶启动子" primer_bind complement(13252..13268) /note="常见的测序引物,多种相似变体之一" f1 ori 13413..13868 /direction=RIGHT /note="f1噬菌体复制起点;箭头表示(+)链合成的方向"
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