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LSL-Cas9-Rosa26TV

产品货号 P10108
产品别称 等效质粒Plasmid#61408
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价格： ¥480.00
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基本信息

产品简介

产品声明

  1. For Research Use Only
    All products available through the MiaoLing Plasmid Platform are intended for research use only. They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.
  2. Plasmid Sequence and Experimental Performance
    Plasmid products are provided and sold based on the corresponding reference/template plasmid. MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.
  3. Sequence Verification of Certain Vector Products
    For certain vector products, even the original manufacturer may not have fully verified the complete plasmid sequence. If the sequence of the key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.
  4. After-Sales Period for Small-Pack Plasmid Products
    The after-sales service period for small-pack plasmid products is three months from the date of receipt. Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.
  5. Research-Related Risks and Limitation of Liability
    Scientific research is inherently exploratory and involves a significant degree of uncertainty. To the maximum extent permitted by applicable law, MiaoLing shall not be liable for any additional economic losses, indirect losses, or other liabilities arising from the use of our products or from research outcomes.
  6. Citation in Chinese Publications
    For publications in Chinese, please cite the product as follows:
    产品名称（产品货号）由淼灵质粒平台提供。
  7. Citation in English Publications
    For publications in English, please cite the product as follows:
    “Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”

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产品文件

P10108_全序列图谱.dna

质粒元件

Rosa26 left arm 3..1085 /note="小鼠Rosa26基因内含子1的左同源臂（Soriano，1999）" CMV enhancer 1181..1484 /note="人类巨细胞病毒即刻早期增强子" chicken beta-actin promoter 1486..1763 intron 1765..2782 /note="鸡β肌动蛋白内含子与兔β珠蛋白嵌合体" FRT (minimal) 2858..2891 /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="支持FLP介导的切除，但不支持整合（Turan和Bode，2011）" loxP complement(2915..2948) /bound_moiety="Cre recombinase" /note="Cre介导的重组发生在8-bp核心序列（GCATACAT）中。" SV40 poly(A) signal 3115..3249 /note="SV40聚腺苷酸化信号" SV40 poly(A) signal 3369..3503 /note="SV40聚腺苷酸化信号" SV40 poly(A) signal 3623..3757 /note="SV40聚腺苷酸化信号" loxP complement(3786..3819) /bound_moiety="Cre recombinase" /note="Cre介导的重组发生在8-bp核心序列（GCATACAT）中。" regulatory 3877..3886 /regulatory_class="other" /note="脊椎动物强启动翻译的共识序列（Kozak，1987）" CDS 3886..3951 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" Cleavage site after base 3951" /translation="DYKDHDGDYKDHDIDYKDDDDK" SV40 NLS 3958..3978 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /translation="PKKKRKV" CDS 4003..8103 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /note="在DNA中产生RNA引导的双链断裂" /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGD" nucleoplasmin NLS 8104..8151 /codon_start=1 /product="bipartite nuclear localization signal from nucleoplasmin" /translation="KRPAATKKAGQAKKKK" P2A 8161..8217 /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /note="真核核糖体不能在Gly和Pro残基之间插入肽键，从而产生单独的多肽。" Cleavage site after base 8214" /translation="ATNFSLLKQAGDVEENPGP" EGFP 8218..8934 /codon_start=1 /product="enhanced GFP" /note="哺乳动物密码子优化" /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" WPRE 8985..9573 /note="土拨鼠肝炎病毒转录后调控元件" Factor Xa site complement(9456..9467) /codon_start=1 /product="Factor Xa recognition and cleavage site" Cleavage site after base 9455" /translation="IEGR" primer_bind complement(9576..9592) /note="常见的测序引物，多种相似变体之一" bGH poly(A) signal 9600..9807 /note="牛生长激素多聚腺苷酸化信号" attB 9815..9848 /bound_moiety="phage phi-C31 integrase" /note="phi-C31整合酶的最小attB位点（Groth等人，2000）" PGK promoter 9870..10369 /note="小鼠磷酸甘油酸激酶1启动子" FRT (minimal) 10389..10422 /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="支持FLP介导的切除，但不支持整合（Turan和Bode，2011）" regulatory 10423..10432 /regulatory_class="other" /note="脊椎动物强启动翻译的共识序列（Kozak，1987）" NeoR/KanR 10429..11232 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /note="赋予对新霉素、卡那霉素和G418（遗传霉素（R））的抗性" /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" PGK poly(A) signal 11242..11703 /note="小鼠磷酸甘油酸激酶1多聚腺苷酸化信号" Rosa26 right arm 11769..16109 /note="小鼠Rosa26基因内含子1的右同源臂（Soriano，1999）" PGK promoter 16159..16658 /note="小鼠磷酸甘油酸激酶1启动子" DTA 16685..17257 /codon_start=1 /gene="Corynebacterium diphtheriae tox" /product="diphtheria toxin fragment A" /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI NFETRGKRGQDAMYEYMAQACAGNRVRR" intron 17334..17399 /note="SV40（猿猴病毒40）小t抗原内含子" bGH poly(A) signal 17473..17697 /note="牛生长激素多聚腺苷酸化信号" T3 promoter complement(17759..17777) /note="噬菌体T3 RNA聚合酶启动子" primer_bind complement(17798..17814) /note="常见的测序引物，多种相似变体之一" lac operator 17822..17838 /bound_moiety="lac repressor encoded by lacI" /note="lac阻遏物与lac操作员结合，抑制大肠杆菌中的转录。这种抑制可以通过添加乳糖或异丙基β-D-硫代吡喃半乳糖苷（IPTG）来缓解。" lac promoter complement(17846..17876) /note="大肠杆菌lac操纵子的启动子" CAP binding site 17891..17912 /bound_moiety="E. coli catabolite activator protein" /note="CAP结合在cAMP存在下激活转录。" ori complement(18200..18788) /direction=LEFT /note="高拷贝数ColE1/pMB1/pBR322/pUC复制起点" AmpR complement(18959..19819) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="对氨苄青霉素、羧苄青霉素和相关抗生素产生耐药性" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" AmpR promoter complement(19820..19924) /gene="bla" f1 ori 19951..20406 /direction=RIGHT /note="f1噬菌体复制起点；箭头表示（+）链合成的方向" primer_bind 20547..20563 /note="常见的测序引物，多种相似变体之一" T7 promoter 20573..20591 /note="噬菌体T7 RNA聚合酶启动子"
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