pAX01-PPK

产品货号 P0543

产品别称整合型木糖诱导

选择规格

少量干粉质粒（需转化提取）转染级质粒100μg（液体即用）转染级质粒200μg（液体即用）转染级质粒500μg（液体即用）

数　量

转染级质粒质检标准查看质粒手册

价格： ¥780.00

联系我们

基本信息
产品简介
产品声明
相关论文
产品文件
质粒元件

基本信息

产品简介

产品声明

For Research Use Only
All products available through the MiaoLing Plasmid Platform are intended for research use only. They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.
Plasmid Sequence and Experimental Performance
Plasmid products are provided and sold based on the corresponding reference/template plasmid. MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.
Sequence Verification of Certain Vector Products
For certain vector products, even the original manufacturer may not have fully verified the complete plasmid sequence. If the sequence of the key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.
After-Sales Period for Small-Pack Plasmid Products
The after-sales service period for small-pack plasmid products is three months from the date of receipt. Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.
Research-Related Risks and Limitation of Liability
Scientific research is inherently exploratory and involves a significant degree of uncertainty. To the maximum extent permitted by applicable law, MiaoLing shall not be liable for any additional economic losses, indirect losses, or other liabilities arising from the use of our products or from research outcomes.
Citation in Chinese Publications
For publications in Chinese, please cite the product as follows:
产品名称（产品货号）由淼灵质粒平台提供。
Citation in English Publications
For publications in English, please cite the product as follows:
“Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”

质粒序列

GGGGTGATGTCAAAGCTTGAAAAAACGCACGTAACAAAAGCAAAATTTATGCTCCATGGGGGAGACTACAACCCCGATCAGTGGCTGGATCGGCCCGATATTTTAGCTGACGATATCAAACTGATGAAGCTTTCTCATACGAATACGTTTTCTGTCGGCATTTTTGCATGGAGCGCACTTGAGCCGGAGGAGGGCGTATATCAATTTGAATGGCTGGATGATATTTTTGAGCGGATTCACAGTATAGGCGGCCGGGTCATATTAGCAACGCCGAGCGGAGCCCGTCCGGCCTGGCTGTCGCAAACCTATCCGGAAGTTTTGCGCGTCAATGCCTCCCGCGTCAAACAGCTGCACGGCGGAAGGCACAACCACTGCCTCACATCTAAAGTCTACCGAGAAAAAACACGGCACATCAACCGCTTATTAGCAGAACGATACGGACATCACCCGGCGCTGTTAATGTGGCACATTTCAAACGAATACGGGGGAGATTGCCACTGTGAATCGATGCGGCCGCTCTAGAGCAACGTTCTTGCCATTGCTGCATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAATTAATTAATCATCGGGAAGATCTTCATCACCGAAACGCGGCAGGCAGCTCTAGAGTTAACAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGCTAGAGCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTCAAGCTAGAGAGTCATTACCAGATCTCACTGCAGAGATCCCCGGGTCTAGAGTCTAGGGACCTCTTTAGCTCCTTGGAAGCTGTCAGTAGTATACCTAATAATTTATCTACATTCCCTTTAGTAACGTGTAACTTTCCAAATTTACAAAAGCGACTCATAGAATTATTTCCTCCCGTTAAATAATAGATAACTATTAAAAATAGACAATACTTGCTCATAAGTAACGGTACTTAAATTGTTTACTTTGGCGTGTTTCATTGCTTGATGAAACTGATTTTTAGTAAACAGTTGACGATATTCTCGATTGACCCATTTTGAAACAAAGTACGTATATAGCTTCCAATATTTATCTGGAACATCTGTGGTATGGCGGGTAAGTTTTATTAAGACACTGTTTACTTTTGGTTTAGGATGAAAGCATTCCGCTGGCAGCTTAAGCAATTGCTGAATCGAGACTTGAGTGTGCAAGAGCAACCCTAGTGTTCGGTGAATATCCAAGGTACGCTTGTAGAATCCTTCTTCAACAATCAGATAGATGTCAGACGCATGGCTTTCAAAAACCACTTTTTTAATAATTTGTGTGCTTAAATGGTAAGGAATACTCCCAACAATTTTATACCTCTGTTTGTTAGGGAATTGAAACTGTAGAATATCTTGGTGAATTAAAGTGACACGAGTATTCAGTTTTAATTTTTCTGACGATAAGTTGAATAGATGACTGTCTAATTCAATAGACGTTACCTGTTTACTTATTTTAGCCAGTTTCGTCGTTAAATGCCCTTTACCTGTTCCAATTTCGTAAACGGTATCGGTTTCTTTTAAATTCAATTGTTTTATTATTTGGTTGAGTACTTTTTCACTCGTTAAAAAGTTTTGAGAATATTTTATATTTTTGTTCATGTAATCACTCCTTCTTAATTACAAATTTTTAGCATCTAATTTAACTTCAATTCCTATTATACAAAATTTTAAGATACTGCACTATCAACACACTCTTAAGTTTGCTTCTAAGTCTTATTTCCATAACTTCTTTTACGTTTCCGCCATTCTTTGCTGTTTCGATTTTTATGATATGGTGCAAGTCAGCACGAACACGAACCGTCTTATCTCCCATTATATCTTTTTTTGCACTGATTGGTGTATCATTTCGTTTTTCTTTTGTGCTAGAGGATCAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTGCGGCCGCCCGCGGTTAATGATGGTGGTGATGATGTTCAGGTTGTTCAAGAGATTTGATGTAATCGTAGATAGCAAGTTGAGCACGAACTTTACGACGGTTGCCACGAGGAACGTAACGGTTAGAAAGTTCTTTATCGATGTAACGAGCTTTAACTGTATCAGAGAAAAGGATATCGATGATATCAAGAACACGTTGTTTAAGACGAGGATCAAGAAGAGGTGTAGCAACTTCGATACGGTAATCGATGTTACGTGTCATCCAATCAGCAGAAGAAAGGTAAACTTTTTTATCGCCGCCGTTTTCGAAGATGTAAACACGATCATGTTCAAGGTAACGATCAACGATAGAGATAGCACGGATGTTATCAGAGATGCCTTCAAGGTTAGGGATAAGAGAGCACATGCCACGAACAAGAAGGTTAACAGGAACGCCAGAAGAAGAAGCAGCGTAAAGACGATCAACAAGGCCTTTATCAACAAGGTTGTTAAGTTTAAGTGTGATGCCAGAAGGAAGGCCTTGTTGAGCGTTAGCGATTTCACGATCAACCATTTCGTAAAGAAGACGACGAGAGTTTTGAGGAGAAACCATAAGGTAATCGAATGTAACAGGACGGTAAGGGTTTTCGATGAAGTTGAAAACACGACGAACTTCGTTTGTGATACGAGCATCAGCTGTAAGAAGAGAGTAATCTGTGTAAAGACGAGCTGTTTTTTCGTTGAAGTTGCCTGTGCCGATATGAGCGTAACGAACAACTTCGCCGTTTTCTTTACGAGAGATAAGGAAAAGTTTAGCATGGATTTTAAGGCCAGGAGCAGAGAAGATAACATGAACGCCAGCTTCTGTAAGACGTTTAGCCCAATGGATGTTAGCTTCTTCATCGAAACGAGCTTGAAGTTCAACAACAACTGTAACTTTTTTGCCGTTATGAGCAGCATGGATCATAGAATCGATGATACGAGAATCTTTAGCAACACGGTAGATGTTGATTTTGATAGCAAGAACAGAAGGATCGAAAGAAGCTTGACGAAGAAGTTCAAGAACATGTTCGAATGTATGGTAAGGGTAGTAAAGAAGAACATCACGTTCACGGATAGCATCGAAGCCGTTACGGAATTGAGCTTTATCGAACCAGATATGACGAAGACGAGGAAGAGGTTTGTTAACAAGGTTAGCTTTGCCAACGTTAGGGAAGTTGATGAAATCTTTGAAGTTATGGTAACGGCCGCCAGGAACGATAGAATCGTAACGAGAGATTGTAAGTTTTTCACGAAGAACTTCAACAAGAGCGTTAGGCATATCACGTTGGTAAACGAAACGAACAGGTTCAGCTGTAAGACGTTGTTTAAGAGAAGAAGACATAAGTTCCATAAGAGAAGCTTCCATTTCATGAACAAGATCGTATTCAGCATCACGTGTCATTTTCATAGAGTAAGCGTTAAGAGCATCGTAATCGAAGAAGCCTTTGAAGATATCATCAAGGCAGTAACGAAGGATGTTATCAAGAAGGATCATAGGTTTACGACGACGAGGAGCTTCAGGAGGAAGGTTAACGAAACGAGGAACTTTATCAGAAGGGATTTCAAGAAGAGCGTAACGGATTGTATCGCCACGGATGATTTCAACAGCAAGGTATGTGTAATCATCTTTAAGGAATTGAACAAGATCTGTATCAGGGTTGATAAGGATAGGTGTGATATGTTGACGAAGGTATTGTTTGAAGTAATGACGAAGCCAGTTTTGTTGGTTAACAGAAAGTTGACGTTCGTTGATAAGGAAGATTTGGTTACGAGCCATTTCAAGAAGAAGTTCGTTGTAAAGGCCATCGAATTCTTGATCAGCTTTAAGAACACGAGATTGGATTTTGCCAAGAAGATGACGAGAATGAGAGTTAGAGCCTTGTTCTTCAGAGATGATGATACGACGTTTAAGTTCAGCGAAACGAACTTTGTAGAATTCATCAAGGTTGTTAGAGTAGATGCCAAGGAAACGCATACGTTCGATAAGAGGGTTAGATTTATCAGCAGCTTCTTGAAGAACACGTTCGTTGAAAGAAAGCCAAGAAAGTTCTTTTTCGATGTAAAGTTTTTCTTGGCCCATGGATCCCATTTCCCCCTTTGATTTTTAGATATCACTAGTTTGGACCATTTGTCATTTCCCCCTTTGATTTAAGTGAACAAGTTTATCCATCAACTATCTTAATTGAGTTAGTTTGTTTATCCAATAAACTAACTTTATCTCATCATATACAAAATAAATGTTTATTTCAATGTTTTTTTTAGAAAATTTAGTTATAATATTAGATATGATACTTTTAAATATCTAATTCAAGCTTCAAAAAACACCAACTTAGTTCGGTGGATAAACAAAGGAGTGGTTATTATTCAAATTGCAGATCAAGCTTTAGTAAAAAAAATGAATCAAAAATTAATATTAGATGAAATTTTGAAGAACTCCCCTGTCTCCAGGGCAACTCTCTCTGAGATTACAGGATTAAACAAGTCTACTGTCTCCTCTCAAGTAAATACACTGCTTGAAAAAGATTTTATTTTTGAAATTGGGGCAGGGCAATCTAGAGGCGGCAGAAGACCTGTAATGCTTGTTTTTAATAAGAATGCAGGCTACTCGATTGGTATTGATATAGGAGTCGACTATCTTAACGGAATTCTAACCGACTTAGAAGGAAATATTATTCTCGAGAAGACTTCTGACTTGTCTAGTTCTTCCGCTAGTGAAGTAAAAGAGATTTTATTTGCACTTATTCATGGTTTTGTAACCCATATGCCTGAGTCCCCTTATGGTCTAGTCGGAATAGGAATTTGTGTTCCAGGCCTTGTAGATCGTCATCAGCAAATTATTTTCATGCCTAACTTAAATTGGAATATCAAAGATTTGCAGTTTTTAATTGAGAGTGAGTTTAATGTTCCGGTTTTTGTTGAAAATGAAGCTAATGCAGGAGCATACGGTGAAAAAGTATTTGGTATGACAAAAAACTATGAAAACATCGTTTACATCAGTATTAATATCGGAATTGGAACTGGACTTGTTATTAACAACGAATTGTATAAAGGTGTTCAGGGTTTTTCTGGGGAAATGGGTCATATGACGATAGATTTTAATGGACCCAAATGCAGCTGTGGAAATCGAGGCTGTTGGGAATTATATGCTTCTGAAAAAGCGTTACTGGCTTCGCTCTCTAAAGAAGAAAAGAATATTTCTCGAAAAGAGATTGTGGAACGCGCAAATAAAAATGATGTAGAAATGTTAAATGCACTTCAAAACTTTGGCTTTTATATCGGAATTGGATTAACCAATATCCTTAATACATTTGATATAGAAGCTGTTATCTTGAGAAATCATATAATTGAATCTCATCCCATTGTTTTAAATACGATTAAAAACGAAGTTTCTTCTAGAGTCCATTCTCATTTAGACAATAAATGTGAACTATTGCCTTCTTCGTTAGGAAAAAATGCACCTGCTTTAGGAGCGGTTTCTATCGTTATTGATTCTTTTTTAAGTGTTACCCCTATAAGTTAGGAGCTCCCCGGGACGTTCTTGCCATTGCTGCATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAATTAATTAATCATCGCGACTGCAGAGATATCGATTTCAAGCTATATTTGGAGTTGAGCCTCTTGAAACGGACACCCTGTATCCGAAGGATCGAAACGCTGTCAGCTACCGCAGCCAAATATATGAAATGAAGGATTATGCAACCGTGATTGATGTAAAGACAGCTTCAGTGGAAGCGGTGTATCAAGAAGATTTTTATGCGCGCACGCCAGCGGTCACAAGCCATGAGTATCAGCAGGGCAAGGCGTATTTTATCGGCGCGCGTTTGGAGGATCAATTTCAGCGTGATTTCTATGAGGGTCTGATCACAGACCTGTCTCTCTCTCCAGTTTTTCCGGTTCGGCACGGAAAAGGCGTCTCCGTACAAGCGAGGCAGGATCAGGACAATGATTATATTTTTGTCATGAATTTCACGGAAGAAAAACAGCTGGTCACGTTTGATCAGAGTGTGAAGGACATAATGACAGGAGACATATTGTCAGGCGACCTGACGATGGAAAAGTATGAAGTGAGAATTGTCGTAAACACACATTAGCCCATCATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGCAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGAAGCGATTCACAGATGTCTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAATGTCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTTGGTCACTTGATGCCTCCGTGTAAGGGGGAATTTCTGTTCATGGGGGTAATGATACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAACATGCCCGGTTACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGGATGCGGCGGGACCAGAGAAAAATCACTCAGGGTCAATGCCAGCGCTTCGTTAATACAGATGTAGGTGTTCCACAGGGTAGCCAGCAGCATCCTGCGATGCAGATCCGGAACATAATGGTGCAGGGCGCTGACTTCCGCGTTTCCAGACTTTACGAAACACGGAAACCGAAGACCATTCATGTTGTTGCTCAGGTCGCAGACGTTTTGCAGCAGCAGTCGCTTCACGTTCGCTCGCGTATCGGTGATTCATTCTGCTAACCAGTAAGGCAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAGGAGCACGATCATGCGCACCCGTGGCCAGGACCCAACGCTGCCCGAGATGCGCCGCGTGCGGCTGCTGGAGATGGCGGACGCGATGGATATGTTCTGCCAAGGGTTGGTTTGCGCATTCACAGTTCTCCGCAAGAATTGATTGGCTCCAATTCTTGGAGTGGTGAATCCGTTAGCGAGGTGCCGCCGGCTTCCATTCAGGTCGAGGTGGCCCGGCTCCATGCACCGCGACGCAACGCGGGGAGGCAGACAAGGTATAGGGCGGCGCCTACAATCCATGCCAACCCGTTCCATGTGCTCGCCGAGGCGGCATAAATCGCCGTGACGATCAGCGGTCCAGTGATCGAAGTTAGGCTGGTAAGAGCCGCGAGCGATCCTTGAAGCTGTCCCTGATGGTCGTCATCTACCTGCCTGGACAGCATGGCCTGCAACGCGGGCATCCCGATGCCGCCGGAAGCGAGAAGAATCATAATGGGGAAGGCCATCCAGCCTCGCGTCGCGACTAAGAAAATGCCGTCAAATCCGCTCGCCATGACTTCACTAACGATGCCTTTGAAAATCTTCAAGTTCTTTTCTACTAATTCAAGGCGTGTCTCACCAGGTTTTTGGTTTGCTCCGGCGCAAATGCAGACAATATCAGGAT

产品文件

P0543_测序报告.zip

质粒元件

                                                            
     terminator      546..580
                     /note="λ噬菌体的最小转录终止子（Scholtissek和Grosse，1987）"
     terminator      660..687
                     /note="大肠杆菌rrnB基因的转录终止子T2"
     terminator      706..792
                     /gene="Escherichia coli rrnB"
                     /note="大肠杆菌rrnB基因的转录终止子T1"
     AmpR promoter        2078..2182
                     /gene="bla"
     PPK             complement(2197..4281)
                     /codon_start=1
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSNN
                     LDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEMAR
                     NQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVEIIR
                     GDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYDALNA
                     YSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVLREKLT
                     ISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFDAIRERD
                     VLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHNGKKVTVV
                     VELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVVRYAHIGTG
                     NFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQNSRRLLYEM
                     VDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGMCSLIPNLEGI
                     SDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEVATPLLDPRLKQ
                     RVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIKSLEQPEHHHHHH
                     "
     6xHis             complement(2200..2217)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /translation="HHHHHH"
     PxylA    complement(4288..4596)
     terminator      5753..5787
                     /note="λ噬菌体的最小转录终止子（Scholtissek和Grosse，1987）"
     AmpR promoter        6431..6535
                     /gene="bla"
     AmpR             6536..7396
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="对氨苄青霉素、羧苄青霉素和相关抗生素产生耐药性"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     ori      7567..8155
                     /direction=RIGHT
                     /note="高拷贝数ColE1/pMB1/pBR322/pUC复制起点"
     bom    8341..8481
                     /note="pBR322迁移区的基础"
     rop             complement(8583..8774)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"                             
                        

pAX01-PPK

基本信息

产品简介

产品声明

质粒序列

相关论文

产品文件

质粒元件

质粒序列