产品声明
For Research Use Only research use only . They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.Plasmid Sequence and Experimental Performance reference/template plasmid . MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.Sequence Verification of Certain Vector Products key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.After-Sales Period for Small-Pack Plasmid Products three months from the date of receipt . Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.Research-Related Risks and Limitation of Liability Citation in Chinese Publications 产品名称（产品货号）由淼灵质粒平台提供。 Citation in English Publications “Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”
质粒序列
一键复制序列（有部分质粒是非全长序列）
TCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTCGAGCTCGGTACCCGCTTTTCGTAGATAATGGAATACAAATGGATATCCAGAGTATACACATGGATAGTATACACTGACACGACAATTCTGTATCTCTTTATGTTAACTACTGTGAGGCGTTAAATAGAGCTTGATATATAAAATGTTACATTTCACAGTCTGAACTTTTGCAGATTACCTAATTTGGTAAGATATTAATTATGAACTGAAAGTTGATGGCATCCCTAAATTTGATGAAAGGGGGATCCCCCGGGTTCGAAGGTACCAAGGAAGCATGCGGTACCCGAATTCCTGAGGTGTCTCACAAGTGCCGTGCAGTCCCGCCCCCACTTGCTTCTCTTTGTGTGTAGTGTACGTACATTATCGAGACCGTTGTTCCCGCCCACCTCGATCCGGTCTAGACTGAGGTGTCTCACAAGTGCCGTGCAGTCCCGCCCCCACTTGCTTCTCTTTGTGTGTAGTGTACGTACATTATCGAGACCGTTGTTCCCGCCCACCTCGATCCGGGGATCCCTGAGGTGTCTCACAAGTGCCGTGCAGTCCCGCCCCCACTTGCTTCTCTTTGTGTGTAGTGTACGTACATTATCGAGACCGTTGTTCCCGCCCACCTCGATCCGGGTCGACCTGAGGTGTCTCACAAGTGCCGTGCAGTCCCGCCCCCACTTGCTTCTCTTTGTGTGTAGTGTACGTACATTATCGAGACCGTTGTTCCCGCCCACCTCGATCCGGGAGCTCCTGAGGTGTCTCACAAGTGCCGTGCAGTCCCGCCCCCACTTGCTTCTCTTTGTGTGTAGTGTACGTACATTATCGAGACCGTTGTTCCCGCCCACCTCGATCCGGTCTAGACTGAGGTGTCTCACAAGTGCCGTGCAGTCCCGCCCCCACTTGCTTCTCTTTGTGTGTAGTGTACGTACATTATCGAGACCGTTGTTCCCGCCCACCTCGATCCGGGGATCCCTGAGGTGTCTCACAAGTGCCGTGCAGTCCCGCCCCCACTTGCTTCTCTTTGTGTGTAGTGTACGTACATTATCGAGACCGTTGTTCCCGCCCACCTCGATCCGGGTCGACCTGAGGTGTCTCACAAGTGCCGTGCAGTCCCGCCCCCACTTGCTTCTCTTTGTGTGTAGTGTACGTACATTATCGAGACCGTTGTTCCCGCCCACCTCGATCCGGGCATGCCTGCAGAAGCTTTTGTGGTTGGGACTTTAGCCAAGGGTATAAAAGACCACCGTCCCCGAATTACCTTTCCTCTTCTTTTCTCTCTCTCCTTGTCAACTCACACCCGAAATCGTTAAGCATTTCCTTCTGAGTATAAGAATCATTCAAAGGCGCGCCACAATGGATAAGAAATACTCCATTGGCCTGGACATCGGAACCAACTCCGTGGGTTGGGCCGTGATCACCGATGAGTACAAGGTGCCCTCTAAGAAATTCAAGGTCCTGGGCAACACCGACCGACACTCCATCAAGAAGAACCTGATCGGCGCTCTGCTCTTCGACTCTGGCGAGACCGCTGAGGCCACCCGACTGAAGCGAACCGCTCGAAGACGATACACCCGAAGAAAGAACCGAATCTGTTACCTGCAGGAGATCTTCTCTAACGAGATGGCCAAGGTGGACGACTCTTTCTTCCACCGACTGGAGGAGTCTTTCCTGGTGGAGGAGGACAAGAAGCACGAGCGACACCCCATCTTCGGCAACATCGTGGACGAGGTGGCCTACCACGAGAAGTACCCCACCATCTACCACCTGCGAAAGAAGCTGGTGGACTCTACCGACAAGGCCGACCTGCGACTGATCTACCTGGCCCTGGCCCACATGATCAAGTTCCGAGGCCACTTCCTGATCGAGGGCGACCTGAACCCCGACAACTCTGACGTGGACAAGCTGTTCATCCAGCTGGTGCAGACCTACAACCAGCTCTTCGAAGAGAACCCCATTAACGCTTCTGGCGTGGATGCTAAGGCCATCCTGTCTGCCCGACTGTCTAAGTCTCGACGACTCGAGAACCTGATTGCTCAGCTCCCCGGAGAGAAGAAGAACGGTCTGTTCGGAAACCTGATTGCTCTGTCCCTGGGTCTCACCCCTAACTTCAAGTCCAACTTCGATCTGGCTGAGGACGCTAAGCTGCAGCTGTCTAAGGACACCTACGACGATGACCTGGATAACCTGCTCGCCCAGATTGGCGACCAGTACGCCGACCTGTTCCTGGCCGCCAAGAACCTGTCTGACGCCATCCTGCTGTCTGACATCCTGCGAGTGAACACCGAGATCACCAAGGCCCCCCTGTCTGCCTCCATGATTAAGCGATACGATGAGCACCACCAGGATCTGACCCTCCTCAAGGCTCTGGTCCGACAGCAGCTGCCCGAGAAGTACAAGGAGATTTTCTTCGACCAGTCTAAGAACGGCTACGCCGGCTACATCGACGGCGGCGCCTCTCAGGAGGAGTTCTACAAGTTCATTAAGCCCATCCTGGAGAAGATGGACGGAACCGAGGAACTGCTCGTGAAGCTGAACCGAGAGGACCTCCTGCGAAAGCAGCGAACCTTCGACAACGGCTCTATCCCCCACCAGATCCACCTGGGCGAGCTGCACGCCATCCTGCGACGACAGGAGGACTTCTACCCCTTCCTGAAGGACAACCGAGAGAAGATCGAGAAGATCCTGACCTTCCGAATCCCCTACTACGTGGGACCCCTGGCCCGAGGAAACTCTCGATTCGCTTGGATGACCCGAAAGTCTGAGGAGACCATTACCCCCTGGAACTTCGAGGAGGTGGTGGATAAGGGCGCCTCTGCTCAGTCTTTCATCGAGCGAATGACCAACTTCGACAAGAACCTCCCCAACGAGAAGGTCCTGCCCAAGCACTCTCTGCTCTACGAGTACTTCACCGTCTACAACGAGCTCACCAAGGTCAAGTACGTGACCGAGGGAATGCGAAAGCCCGCTTTCCTGTCTGGAGAGCAGAAGAAGGCTATTGTGGATCTGCTCTTCAAGACTAACCGAAAGGTCACCGTCAAGCAGCTGAAGGAGGATTACTTCAAGAAGATTGAGTGTTTCGATTCTGTCGAGATCTCCGGCGTCGAGGACCGATTCAACGCCTCTCTGGGTACCTACCACGACCTGCTGAAGATTATCAAGGACAAGGATTTCCTGGATAACGAGGAGAACGAGGATATTCTCGAGGACATTGTCCTGACCCTCACCCTGTTCGAGGATCGAGAGATGATTGAGGAGCGACTCAAGACCTACGCTCACCTGTTCGACGACAAGGTGATGAAGCAGCTGAAGCGACGACGATACACCGGCTGGGGCCGACTGTCTCGAAAGCTGATCAACGGCATCCGAGACAAGCAGTCTGGCAAGACCATCCTGGACTTCCTGAAGTCTGACGGCTTCGCCAACCGAAACTTCATGCAGCTGATCCACGACGACTCTCTGACCTTCAAGGAGGACATCCAGAAGGCCCAGGTGTCTGGCCAGGGCGACTCTCTGCACGAGCACATCGCCAACCTGGCCGGCTCTCCCGCCATTAAGAAAGGTATCCTGCAGACCGTCAAGGTGGTCGATGAGCTCGTCAAGGTGATGGGCCGACACAAGCCCGAGAACATTGTCATTGAGATGGCTCGAGAGAACCAGACTACTCAGAAGGGTCAGAAAAACTCCCGAGAGCGAATGAAGCGAATTGAGGAAGGTATTAAGGAGCTGGGATCCCAGATTCTCAAGGAGCATCCCGTGGAGAACACTCAGCTCCAGAACGAGAAGCTGTACCTGTACTATCTGCAGAACGGTCGAGACATGTACGTCGACCAGGAGCTGGATATCAACCGACTCTCCGACTACGATGTGGACCACATTGTGCCCCAGTCCTTCCTGAAGGACGATTCTATCGATAACAAGGTGCTGACCCGATCCGACAAGAACCGAGGCAAGTCTGACAACGTGCCCTCCGAGGAGGTGGTCAAGAAGATGAAGAACTACTGGCGACAGCTGCTGAACGCCAAGCTGATTACCCAGCGAAAGTTCGACAACCTGACCAAGGCCGAGCGAGGCGGCCTGTCTGAGCTGGACAAGGCCGGCTTCATCAAGCGACAGCTGGTGGAGACCCGACAGATCACCAAGCACGTGGCCCAGATCCTGGACTCTCGAATGAACACCAAGTACGACGAGAACGACAAGCTGATCCGAGAGGTGAAGGTGATCACCCTGAAGTCTAAGCTGGTGTCTGACTTCCGAAAGGACTTCCAGTTCTACAAGGTGCGAGAGATTAACAACTACCACCACGCCCACGATGCCTACCTGAACGCTGTCGTGGGCACCGCCCTCATCAAGAAGTATCCCAAGCTGGAGTCCGAGTTCGTCTACGGCGACTACAAGGTCTACGATGTGCGAAAAATGATTGCCAAGTCCGAGCAGGAGATTGGCAAGGCTACCGCCAAGTACTTCTTCTACTCCAACATTATGAACTTCTTCAAGACCGAGATTACCCTGGCTAACGGCGAGATTCGAAAGCGACCCCTCATTGAGACCAACGGAGAGACCGGTGAGATCGTGTGGGACAAGGGACGAGACTTCGCCACCGTGCGAAAGGTGCTGTCTATGCCCCAGGTGAACATCGTGAAGAAGACCGAGGTGCAGACCGGAGGTTTCTCTAAGGAGTCCATCCTGCCCAAGCGAAACTCTGACAAGCTGATCGCCCGAAAGAAGGACTGGGACCCCAAGAAGTACGGAGGTTTCGACTCTCCCACCGTGGCTTACTCTGTGCTGGTGGTGGCCAAGGTGGAGAAGGGCAAGTCTAAGAAGCTGAAGTCTGTGAAGGAGCTGCTGGGCATTACCATCATGGAGCGATCTTCTTTCGAGAAGAACCCCATTGACTTCCTGGAGGCCAAGGGATACAAGGAGGTGAAGAAAGATCTGATTATCAAGCTCCCCAAGTACTCTCTGTTCGAGCTGGAGAACGGACGAAAGCGAATGCTGGCCTCTGCCGGCGAGCTGCAGAAGGGAAACGAGCTGGCCCTGCCCTCCAAGTACGTCAACTTCCTGTACCTCGCCTCCCATTACGAGAAGCTGAAGGGCTCTCCCGAGGATAACGAGCAGAAGCAGCTCTTCGTGGAGCAGCATAAGCACTACCTGGACGAGATCATCGAGCAGATCTCTGAGTTCTCTAAGCGAGTGATCCTGGCTGACGCCAACCTGGATAAGGTGCTGTCTGCTTACAACAAGCACCGAGACAAGCCCATTCGAGAGCAGGCTGAGAACATCATTCACCTGTTCACCCTGACCAACCTGGGAGCCCCCGCTGCCTTCAAGTACTTCGACACCACCATCGACCGAAAGCGATACACCTCTACCAAGGAGGTGCTGGACGCCACCCTGATCCACCAGTCTATCACCGGCCTGTACGAGACCCGAATCGACCTGTCTCAGCTGGGCGGCGACTCTCGAGCCGACCCCAAGAAGAAGCGAAAGGTGTAAGCTAGCCTCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCTCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGCAAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCACACATTTCCCCGAAAAGTGCCACCTGACGTCCCCAGTTGCAAAAGTTGACACAACTCTAGATCTGCTTCCAAATATAGAATCATAACAAGGGTTAGGGTGTGATTATATAATATTGGTCTTAATTGATGTGCTAGGGCTTTAAAAGTTGGTTAAAATAACGCTCTAATGCCTTTTTAATATATTGTCTTTTTCAAAATCTCAAATCGGACACTTCTTCGTGTATGAGACTCCATTTTTTGGCTCCGTCACGTGATATGTATTATCAGCTATAGTGGTGTAAACAAAGTTTTTTACTAGCTGTAATGGCATTTTGTCGGAGTGGTAAATCGCCTTCTTGTTGTGCGTTCGAGTTCTGGACTCTGCACTGGGCTACTTTGAAAAATACCTCTAATGCGCCGATGGTTTAGTGGTAAAATCCATCGTTGCCATCGATGGGCCCCCGGTTCGATTCCGGGTCGGCGCAGGTTGACCTAGGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGCTTTTTTTTACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCAGATCCTCTAGAGTCGAAGCGGCCGCTATGTCTGATAAAAGGATGTAACATAGGCAAGCTGCTCGTGAGTGTTGAGTACGAACCTTAGATCCAAATCACCCGCACCCACGGATATACTTGCTTGAATATACAGTAGTATGCGGCCGCTTCGACACCATATCATATAAAACTAACAATGCATTGAATTAATACGAAGACTACCCGTTGCTATCTCCACACCGTTATCTCCACGGTCCAAAGGCTGCTCAATGTGCTGCATACGTAACGTGGGGTGCAACCTTGAGCACATAGTACTTTCCGAAAACCGGCGATAATTAAGTGTGCACTCCAACTTTTCACACTGAGCATAAAATGTGGAGAAGAAATCACACTAAAAAGTCAGGTAGACTGGAAAATGCGCCATGAAATAATATCTCTTGCTACAGTAATGCCCAGCATCGAGGGGTATTGTGTCACCAACACTATAGTGGCAGCTGAAGCGCTCGTGATTGTAGTATGAGTCTTTATTGGTGATGGGAAGAGTTCACTCAATATTCTCGTTACTGCCAAAACACCACGGTAATCGGCCAGACACCATGGATGTAGATCACCAAGCCTGTGAATGTTATTCGAGCTAAAATGCACATGGTTGGTGAAAGGAGTAGTTGCTGTCGAATTCCGTCGTCGCCTGAGTCATCATTTATTTACCAGTTGGCCACAAACCCTTGACGATCTCGTATGTCCCCTCCGACATACTCCCGGCCGGCTGGGGTACGTTCGATAGCGCTATCGGCATCGACAAGGTTTGGGTCCCTAGCCGATACCGCACTACCTGAGTCACAATCTTCGGAGGTTTAGTCTTCCACATAGCACGGGCAAAAGTGCGTATATATACAAGAGCGTTTGCCAGCCACAGATTTTCACTCCACACACCACATCACACATACAACCACACACATCCACAATGGAACCCGAAACTAAGAAGACCAAGACTGACTCCAAGAAGATTGTTCTTCTCGGCGGCGACTTCTGTGGCCCCGAGGTGATTGCCGAGGCCGTCAAGGTGCTCAAGTCTGTTGCTGAGGCCTCCGGCACCGAGTTTGTGTTTGAGGACCGACTCATTGGAGGAGCTGCCATTGAGAAGGAGGGCGAGCCCATCACCGACGCTACTCTCGACATCTGCCGAAAGGCTGACTCTATTATGCTCGGTGCTGTCGGAGGCGCTGCCAACACCGTATGGACCACTCCCGACGGACGAACCGACGTGCGACCCGAGCAGGGTCTCCTCAAGCTGCGAAAGGACCTGAACCTGTACGCCAACCTGCGACCCTGCCAGCTGCTGTCGCCCAAGCTCGCCGATCTCTCCCCCATCCGAAACGTTGAGGGCACCGACTTCATCATTGTCCGAGAGCTCGTCGGAGGTATCTACTTTGGAGAGCGAAAGGAGGATGACGGATCTGGCGTCGCTTCCGACACCGAGACCTACTCCGTTCCTGAGGTTGAGCGAATTGCCCGAATGGCCGCCTTCCTGGCCCTTCAGCACAACCCCCCTCTTCCCGTGTGGTCTCTTGACAAGGCCAACGTGCTGGCCTCCTCTCGACTTTGGCGAAAGACTGTCACTCGAGTCCTCAAGGACGAATTCCCCCAGCTCGAGCTCAACCACCAGCTGATCGACTCGGCCGCCATGATCCTCATCAAGCAGCCCTCCAAGATGAATGGTATCATCATCACCACCAACATGTTTGGCGATATCATCTCCGACGAGGCCTCCGTCATCCCCGGTTCTCTGGGTCTGCTGCCCTCCGCCTCTCTGGCTTCTCTGCCCGACACCAACGAGGCGTTCGGTCTGTACGAGCCCTGTCACGGATCTGCCCCCGATCTCGGCAAGCAGAAGGTCAACCCCATTGCCACCATTCTGTCTGCCGCCATGATGCTCAAGTTCTCTCTTAACATGAAGCCCGCCGGTGACGCTGTTGAGGCTGCCGTCAAGGAGTCCGTCGAGGCTGGTATCACTACCGCCGATATCGGAGGCTCTTCCTCCACCTCCGAGGTCGGAGACTTTGTTGCCAACAAGGTCAAGGAGCTGCTCAAGAAGGAGTAAGTCGTTTCTACGACGCATTGATGGAAGGAGCAAACTGACGCGCCTGCGGGTTGGTCTACCGGCAGGGTCCGCTAGTGTATAAGACTCTATAAAAAGGGCCCAGCCCTGCTAATGAAATGATGATTTATAATTTACCGGTGTAGCAACCTTGACTAGAAGAAGCAGATTGGGTGTGTTTGTAGTGGAGGACAGTGGTACGTTTTGGAAACAGTCTTCTTGAAAGTGTCTTGTCTACAGTATATTCACTCATAACCTCAATAGCCAAGGGTGTAGTCGGTTTATTAAAGGAAGGGAGTTGTGGCTGATGTGGATAGATATCTTTAAAGCTGGCGACTGCACCCAACGAGTGTGGTGGTAGCTTGTTACTGTATATTCGGTAAGATATATTTTGTGGGGTTTTTAGTGGTGTTTGGTAGGTTAGTGTCTGGTATATGAGTTGTAGGCATGACAATTTGGAAAGGGGTGGACTTTGGGAATATTGTGGGATTTCAATACCTTAGTTTGTACAGGGTAATTGTTACAAATGATACAAAGAACTGTATTTCTTTTCATTTGTTTTAATTGGTTGTATATCAAGTCCGTTAGACGAGCTCAGTGCCATGGCTTTTGGCACTGTATTTCATTTTTAGAGGTACACTACATCCAGTGAGGTATGGTAAGGTTGAGGGCATAATGAAGGCACCTTGTACTGACAGTCACAGACCTCTCACCGAGAATTTTATGAGATATACTCGGGTTCATTTTAGGCTCCGATTCGATTCAAATTATTACTGTCGAAATCGGTTGAGCATCCGTTGATTTCCGAACAGATCTGGCCCTTTCGTC
质粒元件
primer_bind 379..395
/note="通用测序引物，多个类似变体之一"
CDS 1740..5843
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/note="在DNA中产生RNA引导的双链断裂"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
SV40 NLS 5856..5876
/codon_start=1
/product="nuclear localization signal of SV40 large T
antigen"
/translation="PKKKRKV"
terminator 5887..6134
/gene="S. cerevisiae CYC1"
/note="CYC1的转录终止子"
primer_bind complement(6153..6169)
/note="通用测序引物，多个类似变体之一"
lac operator 6177..6193
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
lac promoter complement(6201..6231)
/note="promoter for the E. coli lac operon"
CAP binding site 6246..6267
/bound_moiety="E. coli catabolite activator protein"
/note="CAP结合激活了cAMP存在的转录。"
ori complement(6555..7143)
/direction=LEFT
/note="高拷贝数 ColE1/pMB1/pBR322/pUC 复制起点"
AmpR complement(7314..8174)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="赋予对氨苄西林、卡那西林和相关抗生素的抗性。"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
AmpR promoter complement(8175..8278)
/gene="bla"
misc_RNA 8755..8830
/note="链球菌CRISPR/Cas9系统的导向RNA骨架"
