产品声明
For Research Use Only research use only . They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.Plasmid Sequence and Experimental Performance reference/template plasmid . MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.Sequence Verification of Certain Vector Products key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.After-Sales Period for Small-Pack Plasmid Products three months from the date of receipt . Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.Research-Related Risks and Limitation of Liability Citation in Chinese Publications 产品名称（产品货号）由淼灵质粒平台提供。 Citation in English Publications “Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”
质粒序列
一键复制序列（有部分质粒是非全长序列）
TTGGGGGGGCAGGAGCCCCGCGGGGGGTGGTCGGGTCAGGCGCCGAACCGGCGGGCGGCGGCGGCGACCAGGCCGTCGGCGGCGGCCATGGCGCGGTCGCGGTCGGTGGTGAGGGCGGTGCGGTCGGCGGCGGCCCAGTCCTGCTCTCACGCAACGTCTACGTGGACGCTCAGGGCGACACGATCGAGGTCGGGGAGTCCGTCGCTCCGGCGGGCCGCTGGCGCGTCCACCGGGACTGATCAAGGCGAATACTTCATATGCGGGGATCGACCGCGCGGGTCCCGGACGGGGAAGAGCGGGGAGCTTTGCCAGAGAGCGACGACTTCCCCTTGCGTTGGTGATTGCCGGTCAGGGCAGCCATCCGCCATCGTCGCGTAGGGTGTCACACCCCAGGAATCGCGTCACTGAACACAGCAGCCGGTAGGACGACCATGACTGAGTTGGACACCATCGCAAATCCGTCCGATCCCGCGGTGCAGCGGATCATCGATGTCACCAAGCCGTCGCGATCCAACATAAAGACAACGTTGATCGAGGACGTCGAGCCCCTCATGCACAGCATCGCGGCCGGGGTGGAGTTCATCGAGGTCTACGGCAGCGACAGCAGTCCTTTTCCATCTGAGTTGCTGGATCTGTGCGGGCGGCAGAACATACCGGTCCGCCTCATCGACTCCTCGATCGTCAACCAGTTGTTCAAGGGGGAGCGGAAGGCCAAGACATTCGGCATCGCCCGCGTCCCTCGCCCGGCCAGGTTCGGCGATATCGCGAGCCGGCGTGGGGACGTCGTCGTTCTCGACGGGGTGAAGATCGTCGGGAACATCGGCGCGATAGTACGCACGTCGCTCGCGCTCGGAGCGTCGGGGATCATCCTGGTCGACAGTGACATCACCAGCATCGCGGACCGGCGTCTCCAAAGGGCCAGCCGAGGTTACGTCTTCTCCCTTCCCGTCGTTCTCTCCGGTCGCGAGGAGGCCATCGCCTTCATTCGGGACAGCGGTATGCAGCTGATGACGCTCAAGGCGGATGGCGACATTTCCGTGAAGGAACTCGGGGACAATCCGGATCGGCTGGCCTTGCTGTTCGGCAGCGAAAAGGGTGGGCCTTCCGACCTGTTCGAGGAGGCGTCTTCCGCCTCGGTTTCCATCCCCATGATGAGCCAGACCGAGTCTCTCAACGTTTCCGTTTCCCTCGGAATCGCGCTGCACGAGAGGATCGACAGGAATCTCGCGGCCAACCGATAAGCGCCTCTGTTCCTCGGACGCTCGGTTCCTCGACCTCGATTCGTCAGTGATGATCAGACGTCGAAGGTGTAGTGCCGGGTGTGGTCCAGCAGGGCCGCCGGGGTGGTGTCGTTCCACGGCTTCATCGTCTCGTTGAGGTCGACCAAAGCGGCCATCGTGCCTCCCCACTCCTGCAGATCCCTGCAGGTCGACGGATCTTTTCCGCTGCATAACCCTGCTTCGGGGTCATTATAGCGATTTTTTCGGTATATCCATCCTTTTTCGCACGATATACAGGATTTTGCCAAAGGGTTCGTGTAGACTTTCCTTGGTGTATCCAACGGCGTCAGCCGGGCAGGATAGGTGAAGTAGGCCCACCCGCGAGCGGGTGTTCCTTCTTCACTGTCCCTTATTCGCACCTGGCGGTGCTCAACGGGAATCCTGCTCTGCGAGGCTGGCCGGCTACCGCCGGCGTAACAGATGAGGGCAAGCGGATGGCTGATGAAACCAAGCCAACCAGGAAGGGCAGCCCACCTATCAAGGTGTACTGCCTTCCAGACGAACGAAGAGCGATTGAGGAAAAGGCGGCGGCGGCCGGCATGAGCCTGTCGGCCTACCTGCTGGCCGTCGGCCAGGGCTACAAAATCACGGGCGTCGTGGACTATGAGCACGTCCGCGAGCTGGCCCGCATCAATGGCGACCTGGGCCGCCTGGGCGGCCTGCTGAAACTCTGGCTCACCGACGACCCGCGCACGGCGCGGTTCGGTGATGCCACGATCCTCGCCCTGCTGGCGAAGATCGAAGAGAAGCAGGACGAGCTTGGCAAGGTCATGATGGGCGTGGTCCGCCCGAGGGCAGAGCCATGACTTTTTTAGCCGCTAAAACGGCCGGGGGGTGCGCGTGATTGCCAAGCACGTCCCCATGCGCTCCATCAAGAAGAGCGACTTCGCGGAGCTGGTGAAGTACATCACCGACGAGCAAGGCAAGACCGATCCCCGGGGACCTGCAGGTCGACTCTAGCTAGACACGTCTGAAGCTAGCTTCGAGGAACTTTCGGCGGCTTTGCTGTGCGACAGGCTCACGTCTAAAAGGAAATAAATCATGGGTCATAAAATTATCACGTTGTCCGGCGCGGCGACGGATGTTCTGTATGCGCTGTTTTTCCGTGGCGCGTTGCTGTCTGGTGATCTGCCTTCTAAATCTGGCACAGCCGAATTGCGCGAGCTTGGTTTTGCTGAAACCAGACACACAGCAACTGAATACCAGAAAGAAAATCACTTTACCTTTCTGACATCAGAAGGGCAGAAATTTGCCGTTGAACACCTGGTCAATACGCGTTTTGGTGAGCAGCAATATTGCGCTTCGATGACGCTTGGCGTTGAGATTGATACCTCTGCTGCACAAAAGGCAATCGACGAGCTGGACCAGCGCATTCGTGACACCGTCTCCTTCGAACTTATTCGCAATGGAGTGTCATTCATCAAGGACGCCGCTATCGCAAATGGTGCTATCCACGCAGCGGCAATCGAAACACCTCAGCCGGTGACCAATATCTACAACATCAGCCTTGGTATCCAGCGTGATGAGCCAGCGCAGAACAAGGTAACCGTCAGTGCCGATAAGTTCAAAGTTAAACCTGGTGTTGATACCAACATTGAAACGTTGATCGAAAACGCGCTGAAAAACGCTGCTGAATGTGCGGCGCTGGATGTCACAAAGCAAATGGCAGCAGACAAGAAAGCGATGGATGAACTGGCTTCCTATGTCCGCACGGCCATCATGATGGAATGTTTCCCCGGTGGTGTTATCTGGCAGCAGTGCCGTCGATAGTATGCAATTGATAATTATTATCATTTGCGGGTCCTTTCCGGCGATCCGCCTTGTTACGGGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGCGTTTCCGTTCTTCTTCGTCATAACTTAATGTTTTTATTTAAAATACCCTCTGAAAAGAAAGGAAACGACAGGTGCTGAAAGCGAGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGTGGAATGAACAATGGAAGTCAACAAAAAGCAGAGCTTATCGATGATAAGCGGTCAAACATGAGAATTGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCGCGCAATTAACCCTCACTAAAGGGAACAAAAGCTGGAGCTCCACCGCGGTGGCGGCCGCTCTAGAACTAGTGGATCCCCCGGGCTGCAGGAATTCGATATCAAGCTTATCGATACCGTCGACCTCGAGGGGGGGCCCGGTACCCAATTCGCCCTATAGTGAGTCGTATTACGCGCGCTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCAATTCTTGAAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGCAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCTGACTTCCGCGTTTCCAGACTTTACGAAACACGGAAACCGAAGACCATTCATGTTGTTGCTCAGGTCGCAGACGTTTTGCAGCAGCAGTCGCTTCACGTTCGCTCGCGTATCGGTGATTCATTCTGCTAACCAGTAAGGCAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAGGAGCACGATCATGCGCACCCGTCAGATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTCCCGCACGATCAGCGTCCCGCCGACCAGGAGCAGTGCGGCCAGCACTGCCGCTAACGCCTGGTCCTGGTCCCGGTCCTGGTGGTGCATCAGTCCTCCCCGTGATCACTTCGGCACCCACCGTAGTGATCACCCCCGACAGCGGATCAAGGGGTTTGCGGGTCCCGGTCGGCGCCGGGCGGGGGAGGCAGGAGCCGCCGACGCTGCCTCTGGGACGGGCCGGACGGCAGGGGGACCGGCGGCCGGGCGAGCTGCAGCCGGGGGTCCGGCAGGGCCGGAGCGGGCGGAACCGTGCTCTGACCTGCGGCCCGAGTTTCGTCACGTGACGGAATGGAAGGCTGCTGCATTTCGTCACGTGACGTATCTCGGCGAGCGACTGCCGACGCCACGGCGGACACGATCGCCTCGCGCTGGCGCCGGGCCTCGTACGCCCGCTGGCGGCAGGAGCGGCGGCAGTAGTCCCGGCTCCGGCCGACGCCGGATTGCTTGATCTCCGAGCCGCACCAGGCGCAGAGCTTCGCGCCGTCGGCGTCCCTGGGGGTGGTGGTGCTCATGGCCGACGACCGTACGCGGCACGTCTCGTAGCGAGGCGAGTCGGGCGCGACGCCTGCACGAAGTGCCGGCGGGGCCGACCCCGGGCGAGTAATCCCAGGATTACTCCCGCGGCTTCGACCCCGGCCGCCGTCGCCGCGTACGTCACCGACCCCCGCCGTACGTCACCGGGATGACGTACGGCGGGGGGGAGCGAGTTAGTGCGAAGTGGGCCCACTTGCGAGCCGGGCGATGTGCCGGGCGGCCCGCTCCTGGCGGTCGTCGGCGTCGTCGTCCTGGTCGTCGTCCTGCTCTCGCCGTCGGCGTGCAGTTGCTTCCTCGCGGCGCTGGGCGAGGGCGGCGAGCATGTCGGCGTACGCCTCGGCCACCTCCCCCGCCGTGAGCACCACCACTGTGTCGGCCGCGTCGGCCAGCGCCAGGACCTCCCGCACCCGTTCGCCCACGGCCGCCGAATCCTCGTTGCCGTCCTTGCCTTCGGCGGCCCGGGTCGCCTCGAGGTCGAGGGCGCGGCGGGTGACCGCGTGCCATCCGTCCTCGGTCACGGCGACCCCGGCCCGCAGCTCCCCGCCGTCGGCGTCGGCCGCCAGGAGCAGATCGAGGTCGTCGGCCTCGGTGTCGCCGCCGTCGAGCCCGAGCATCTGCCGCAGGTAGCGGGTCCATTCGATGGCCCGGCGTCCCCGGGTTGCCCGCTCGTACTCGTGCCAGCGCGAGAGGTTCCACTCCAGCGAGCCGACCCCGGCGGCGTCGTCCTCGGTCATGCCGCCGGTCAGGTCCCCGATCCGTCCGAGGAGTTCGAACGGGGCGACGTTCCCGCCGGTCGCCGTCTTGAGGTCGGCGCGGGCGAGTTCGAGGGCGGGCGCCTTCCCGTCCTGGGTCTTGGCGATGTACTCGGCGAGGTCGTTGGCGTCGCGCTCGGTCTCCAGCCGCTTGAAGTCGACGCCGTGCCGGTCGTCGGGCGTGAAGGCGGGGTTGACCTTGCGCAGGGCGGCGGTCCACACGGACCGCCAGTGCCCCTGCCACTCGTCGAGCGCGGCGCCGGTCGGCTCGAAGGTGGCGACGATCTGCTTCGCGGACCGCTCCCCCTCGGTCCGGCCGCCGACCAGGACGATCGCGTGGATGTGCGGGTGCCAGCCGTTGATCTGCCCCACGGTGACTTCGGTCGCGCGGATCATGCCGACGTACCCGATCCGGTCTCGGATGCCCTCGCGGTCGGCGGCCCGGTGCCCGTCCTTGGCCCGGCGTCCGGCCCACGTGCCGCCCGTGATCAGTCGCTGGTAGGCGCCCGGCCGCCGGGGGCTGTCCGGCGTCTTCCGGGTGCCCTGGAGGGCGTCCATGAGGTCCGCGAGCCGGTCCGTGTGCCCATGGCGGGCCGTGAAGGTGACCAGGTAGGCGGTCCCCCCGCGCTTGATCCACTCGACCACGGCGGCGGTGATCTCCTCGGCCCGCTTGTGCCGGATCGTGGCGGCGCAGACCGGGCAGAGCCAGATCCGCCCGCACCGCATCAGGCCCAGGACCACGGACGTTCCGGCCGCCGTCTGGGCGACGATCACGCCGGAGGCAGGGTCCATCAGGGCGCGGCCGCAGCCCTTGCACGCGGCGTCCCCGCTGATCCGCCACAGCGTCCGGCGGCGGCTGTACCGGGCGGCTTTCCGCAGTCGGGCAGCGTCGCTCCGCGACGTGCTTCCTACTTCCGAGAGGCTGTCGCCTCTCGGGCTCTCCCCATCCACCCCGTCCGGAGAAACCGCAGGTCGGAGGGGTGCGGGAAACTCTGTTGTTTCTTTCCCAAGGTGTTCGCTTTTGCCTCGGGCGGCATCTCGCGTCACACGCGCGATCGCCCGCTTCGCTGCCATCCGGCAGCGGTCTGAGCAGTAGATACGCGGCCGTTTGCCCGGTGTGTGGGCAATTGCGGTCCCGCAGTGGCAGCGGGGCCCGGCGGGCCGATCTGGCAATGCCTCGGCATCGCTCCGTACTCTGGGCACGAGCAACGTTCCTGTCTCGCCCGGCTAAGGGGCGCGAGTCTGGGAGCGGACGGGTCGGAGGTGCGAAGTCCGGCCCGTTGCTCTTTGGTCTGGTGGGAATCCTGGCACCAATCGGGCCAGAGGTTCCCTCCGCCACTCCCGACGCCCCTTGGGGCTGGTGTGACTTGGAGGGCCGAAGAGAGCCCCGCCGGTGATCCGGCGGGGCTTTGACGTGCGGTCAGTGCGTGTGTCGGCGAACGATGGCCACAAAGCCCTGGAAGCCGAGCGGTCCGGCAAAGTCGGCCCAGTCCAACCGGGCTCAGCGC
质粒元件
oriT 1573..1682
/note="incP转移起始位点"
traJ 1715..2086
/codon_start=1
/gene="traJ"
/product="oriT-recognizing protein"
/translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
KQDELGKVMMGVVRPRAEP"
CAP binding site 3457..3478
/bound_moiety="E. coli catabolite activator protein"
/note="CAP结合激活了cAMP存在的条件下的转录。"
lac promoter 3493..3523
/note="大肠杆菌乳糖操纵子的启动子"
lac operator 3531..3547
/bound_moiety="lac repressor encoded by lacI"
/note="lac阻遏蛋白结合到lac操纵子上以抑制大肠杆菌中的转录。这种抑制可以通过添加乳糖或异丙基-β-D-硫代半乳糖苷（IPTG）来解除。"
primer_bind 3555..3571
/note="通用测序引物，多个类似变体之一"
lacZ-alpha 3567..3974
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLSI
PSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
TDRPSQQLRSLNGEWQFLKTKGPRDTPIFIG"
T3 promoter 3592..3610
/note="T3 噬菌体 RNA 聚合酶的启动子"
MCS 3623..3730
/note="pBluescript 多克隆位点"
primer_bind 3647..3663
/note="通用测序引物，多个类似变体之一"
primer_bind complement(3697..3713)
/note="通用测序引物，多个类似变体之一"
T7 promoter complement(3739..3757)
/note="1型人类免疫缺陷病毒RNA聚合酶启动子"
primer_bind complement(3767..3783)
/note="通用测序引物，多个类似变体之一"
AmpR promoter 4030..4134
/gene="bla"
AmpR 4135..4995
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="赋予对氨苄西林、卡那西林和相关抗生素的抗性。"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
ori 5166..5754
/direction=RIGHT
/note="高拷贝数 ColE1/pMB1/pBR322/pUC 复制起点"
SV40 poly(A) signal 6139..6273
/note="SV40 polyA信号"
