产品声明
For Research Use Only research use only . They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.Plasmid Sequence and Experimental Performance reference/template plasmid . MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.Sequence Verification of Certain Vector Products key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.After-Sales Period for Small-Pack Plasmid Products three months from the date of receipt . Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.Research-Related Risks and Limitation of Liability Citation in Chinese Publications 产品名称（产品货号）由淼灵质粒平台提供。 Citation in English Publications “Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”
质粒序列
一键复制序列（有部分质粒是非全长序列）
AGATCTAACATCCAAAGACGAAAGGTTGAATGAAACCTTTTTGCCATCCGACATCCACAGGTCCATTCTCACACATAAGTGCCAAACGCAACAGGAGGGGATACACTAGCAGCAGACCGTTGCAAACGCAGGACCTCCACTCCTCTTCTCCTCAACACCCACTTTTGCCATCGAAAAACCAGCCCAGTTATTGGGCTTGATTGGAGCTCGCTCATTCCAATTCCTTCTATTAGGCTACTAACACCATGACTTTATTAGCCTGTCTATCCTGGCCCCCCTGGCGAGGTTCATGTTTGTTTATTTCCGAATGCAACAAGCTCCGCATTACACCCGAACATCACTCCAGATGAGGGCTTTCTGAGTGTGGGGTCAAATAGTTTCATGTTCCCCAAATGGCCCAAAACTGACAGTTTAAACGCTGTCTTGGAACCTAATATGACAAAAGCGTGATCTCATCCAAGATGAACTAAGTTTGGTTCGTTGAAATGCTAACGGCCAGTTGGTCAAAAAGAAACTTCCAAAAGTCGGCATACCGTTTGTCTTGTTTGGTATTGATTGACGAATGCTCAAAAATAATCTCATTAATGCTTAGCGCAGTCTCTCTATCGCTTCTGAACCCCGGTGCACCTGTGCCGAAACGCAAATGGGGAAACACCCGCTTTTTGGATGATTATGCATTGTCTCCACATTGTATGCTTCCAAGATTCTGGTGGGAATACTGCTGATAGCCTAACGTTCATGATCAAAATTTAACTGTTCTAACCCCTACTTGACAGCAATATATAAACAGAAGGAAGCTGCCCTGTCTTAAACCTTTTTTTTTATCATCATTATTAGCTTACTTTCATAATTGCGACTGGTTCCAATTGACAAGCTTTTGATTTTAACGACTTTTAACGACAACTTGAGAAGATCAAAAAACAACTAATTATTCGAAACGGAATTCCGGACCGGCATGCAGGCCTGGTACCGGCCGGCCATTTAAATACAGGCCCCTTTTCCTTTGTCGATATCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCTCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTTAATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAAACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGCAAGCTGGATCCGCGGCCGCGACTTGACTCTTCACTAGCCTATGCAAATAAGGTTACCTTTTCCAAGAATCGTAGAAACGATTAAAAAACTTCCAAACTCTCATGGATTCTCAGGTAATAGGTATTCTAGGAGGAGGCCAGCTAGGCCGAATGATTGTTGAGGCCGCTAGCAGGCTCAATATCAAGACCGTGATTCTTGATGATGGTTTTTCACCTGCTAAGCACATTAATGCTGCGCAAGACCACATCGACGGATCATTCAAAGATGAGGAGGCTATCGCCAAGTTAGCTGCCAAATGTGATGTTCTCACTGTAGAGATTGAGCATGTCAACACAGATGCTCTAAAGAGAGTTCAAGACAGAACTGGAATCAAGATATATCCTTTACCAGAGACAATCGAACTAATCAAGGATAAGTACTTGCAAAAGGAACATTTGATCAAGCACAACATTTCGGTGACAAAGTCTCAGGGTATAGAATCTAATGAAAAGGCGCTGCTTTTGTTTGGAGAAGAGAATGGATTTCCATATCTGTTGAAGTCCCGGACTATGGCTTATGATGGAAGAGGCAATTTTGTAGTGGAGTCTAAAGAGGACATCAGTAAGGCATTAGAGTTCTTGAAAGATCGTCCATTGTATGCCGAGAAGTTTGCTCCTTTTGTTAAAGAATTAGCGGTAATGGTTGTGAGATCACTGGAAGGCGAAGTATTCTCCTACCCAACCGTAGAAACTGTGCACAAGGACAATATCTGTCATATTGTGTATGCTCCGGCCAGAGTTAATGACACCATCCAAAAGAAAGCTCAAATATTAGCTGAAAACACTGTGAAGACTTTCCCAGGCGCTGGAATCTTCGGAGTTGAGATGTTCCTATTGTCTGATGGAGAACTTCTTGTAAATGAGATTGCTCCAAGGCCCCACAATTCTGGTCACTATACAATCGATGCATGTGTAACATCTCAGTTCGAAGCACATGTAAGAGCCATAACTGGTCTGCCAATGCCACTAGATTTCACCAAACTATCTACTTCCAACACCAACGCTATTATGCTCAATGTTTTGGGTGCTGAAAAATCTCACGGGGAATTAGAGTTTTGTAGAAGAGCCTTAGAAACACCCGGTGCTTCTGTATATCTGTACGGAAAGACCACCCGATTGGCTCGTAAGATGGGTCATATCAACATAATAGGATCTTCCATGTTGGAAGCAGAACAAAAGTTAGAGTACATTCTAGAAGAATCAACCCACTTACCATCCAGTACTGTATCAGCTGACACTAAACCGTTGGTTGGAGTTATCATGGGTTCAGACTCTGATCTACCTGTGATTTCGAAAGGTTGCGATATTTTAAAACAGTTTGGTGTTCCATTCGAAGTTACTATTGTCTCTGCTCATAGAACACCACAGAGAATGACCAGATATGCCTTTGAAGCCGCTAGTAGAGGTATCAAGGCTATCATTGCAGGTGCTGGTGGTGCTGCTCATCTTCCAGGAATGGTTGCTGCCATGACTCCGTTGCCAGTCATTGGTGTTCCTGTCAAGGGCTCTACGTTGGATGGTGTAGACTCGCTACACTCGATTGTCCAAATGCCTAGAGGTGTTCCTGTGGCTACGGTTGCTATCAACAACGCCACCAATGCCGCTCTGTTGGCCATCAGGATTTTAGGTACAATTGACCACAAATGGCAAAAGGAAATGTCCAAGTATATGAATGCAATGGAGACCGAAGTGTTGGGGAAGGCATCCAACTTGGAATCTGAAGGGTATGAATCCTATTTGAAGAATCGTCTTTGAATTTAGTATTGTTTTTTAATAGATGTATATATAATAGTACACGTAACTTATCTATTCCATTCATAATTTTATTTTAAAGGTTCGGTAGAAATTTGTCCTCCAAAAAGTTGGTTAGAGCCTGGCAGTTTTGATAGGCATTATTATAGATTGGGTAATATTTACCCTGCACCTGGAGGAACTTTGCAAAGAGCCTCATGTGCGGCGCGCCAGGCCATAATGGCCAAACGGTTTCTCAATTACTATATACTACTAACCATTTACCTGTAGCGTATTTCTTTTCCCTCTTCGCGAAAGCTCAAGGGCATCTTCTTGACTCATGAAAAATATCTGGATTTCTTCTGACAGATCATCACCCTTGAGCCCAACTCTCTAGCCTATGAGTGTAAGTGATAGTCATCTTGCAACAGATTATTTTGGAACGCAACTAACAAAGCAGATACACCCTTCAGCAGAATCCTTTCTGGATATTGTGAAGAATGATCGCCAAAGTCACAGTCCTGAGACAGTTCCTAATCTTTACCCCATTTACAAGTTCATCCAATCAGACTTCTTAACGCCTCATCTGGCTTATATCAAGCTTACCAACAGTTCAGAAACTCCCAGTCCAAGTTTCTTGCTTGAAAGTGCGAAGAATGGTGACACCGTTGACAGGTACACCTTTATGGGACATTCCCCCAGAAAAATAATCAAGACTGGGCCTTTAGAGGGTGCTGAAGTTGACCCCTTGGTGCTTCTGGAAAAAGAACTGAAGGGCACCAGACAAGCGCAACTTCCTGGTATTCCTCGTCTAAGTGGTGGTGCCATAGGATACATCTCGTACGATTGTATTAAGTACTTTGAACCAAAAACTGAAAGAAAACTGAAAGATGTTTTGCAACTTCCGGAAGCAGCTTTGATGTTGTTCGACACGATCGTGGCTTTTGACAATGTTTATCAAAGATTCCAGGTAATTGGAAACGTTTCTCTATCCGTTGATGACTCGGACGAAGCTATTCTTGAGAAATATTATAAGACAAGAGAAGAAGTGGAAAAGATCAGTAAAGTGGTATTTGACAATAAAACTGTTCCCTACTATGAACAGAAAGATATTATTCAAGGCCAAACGTTCACCTCTAATATTGGTCAGGAAGGGTATGAAAACCATGTTCGCAAGCTGAAAGAACATATTCTGAAAGGAGACATCTTCCAAGCTGTTCCCTCTCAAAGGGTAGCCAGGCCGACCTCATTGCACCCTTTCAACATCTATCGTCATTTGAGAACTGTCAATCCTTCTCCATACATGTTCTATATTGACTATCTAGACTTCCAAGTTGTTGGTGCTTCACCTGAATTACTAGTTAAATCCGACAACAACAACAAAATCATCACACATCCTATTGCTGGAACTCTTCCCAGAGGTAAAACTATCGAAGAGGACGACAATTATGCTAAGCAATTGAAGTCGTCTTTGAAAGACAGGGCCGAGCACGTCATGCTGGTAGATTTGGCCAGAAATGATATTAACCGTGTGTGTGAGCCCACCAGTACCACGGTTGATCGTTTATTGACTGTGGAGAGATTTTCTCATGTGATGCATCTTGTGTCAGAAGTCAGTGGAACATTGAGACCAAACAAGACTCGCTTCGATGCTTTCAGATCCATTTTCCCAGCAGGAACCGTCTCCGGTGCTCCGAAGGTAAGAGCAATGCAACTCATAGGAGAATTGGAAGGAGAAAAGAGAGGTGTTTATGCGGGGGCCGTAGGACACTGGTCGTACGATGGAAAATCGATGGACACATGTATTGCCTTAAGAACAATGGTCGTCAAGGACGGTGTCGCTTACCTTCAAGCCGGAGGTGGAATTGTCTACGATTCTGACCCCTATGACGAGTACATCGAAACCATGAACAAAATGAGATCCAACAATAACACCATCTTGGAGGCTGAGAAAATCTGGACCGATAGGTTGGCCAGAGACGAGAATCAAAGTGAATCCGAAGAAAACGATCAATGAACGGAGGACGTAAGTAGGAATTTATGGTTTGGCCATAATGGCCTAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTG
质粒元件
AOX1 promoter 2..940
/gene="Pichia pastoris AOX1"
/note="可诱导启动子，受甲醇调控。"
terminator 1013..1262
/gene="S. cerevisiae CYC1"
/note="CYC1的转录终止子"
PpADE2 LC promoter 1282..1363
/gene="Pichia pastoris ADE2"
/note="强82-bp启动子片段，仅需低拷贝整合即可实现高效表达。"
CDS 1364..3055
/codon_start=1
/gene="Pichia pastoris ADE2"
/product="phosphoribosylaminoimidazole carboxylase from
Pichia pastoris"
/note="对嘌呤核苷酸的生物合成至关重要，包括腺嘌呤。"
/translation="MDSQVIGILGGGQLGRMIVEAASRLNIKTVILDDGFSPAKHINAA
QDHIDGSFKDEEAIAKLAAKCDVLTVEIEHVNTDALKRVQDRTGIKIYPLPETIELIKD
KYLQKEHLIKHNISVTKSQGIESNEKALLLFGEENGFPYLLKSRTMAYDGRGNFVVESK
EDISKALEFLKDRPLYAEKFAPFVKELAVMVVRSLEGEVFSYPTVETVHKDNICHIVYA
PARVNDTIQKKAQILAENTVKTFPGAGIFGVEMFLLSDGELLVNEIAPRPHNSGHYTID
ACVTSQFEAHVRAITGLPMPLDFTKLSTSNTNAIMLNVLGAEKSHGELEFCRRALETPG
ASVYLYGKTTRLARKMGHINIIGSSMLEAEQKLEYILEESTHLPSSTVSADTKPLVGVI
MGSDSDLPVISKGCDILKQFGVPFEVTIVSAHRTPQRMTRYAFEAASRGIKAIIAGAGG
AAHLPGMVAAMTPLPVIGVPVKGSTLDGVDSLHSIVQMPRGVPVATVAINNATNAALLA
IRILGTIDHKWQKEMSKYMNAMETEVLGKASNLESEGYESYLKNRL"
PpTRP2 promoter 3282..3431
/gene="Pichia pastoris TRP2"
/note="启动子用于毕赤酵母TRP2"
PpTRP2 3432..5048
/codon_start=1
/gene="Pichia pastoris TRP2"
/product="anthranilate synthase from Pichia pastoris"
/note="催化色氨酸生物合成的初始步骤。"
/translation="MSVSDSHLATDYFGTQLTKQIHPSAESFLDIVKNDRQSHSPETVP
NLYPIYKFIQSDFLTPHLAYIKLTNSSETPSPSFLLESAKNGDTVDRYTFMGHSPRKII
KTGPLEGAEVDPLVLLEKELKGTRQAQLPGIPRLSGGAIGYISYDCIKYFEPKTERKLK
DVLQLPEAALMLFDTIVAFDNVYQRFQVIGNVSLSVDDSDEAILEKYYKTREEVEKISK
VVFDNKTVPYYEQKDIIQGQTFTSNIGQEGYENHVRKLKEHILKGDIFQAVPSQRVARP
TSLHPFNIYRHLRTVNPSPYMFYIDYLDFQVVGASPELLVKSDNNNKIITHPIAGTLPR
GKTIEEDDNYAKQLKSSLKDRAEHVMLVDLARNDINRVCEPTSTTVDRLLTVERFSHVM
HLVSEVSGTLRPNKTRFDAFRSIFPAGTVSGAPKVRAMQLIGELEGEKRGVYAGAVGHW
SYDGKSMDTCIALRTMVVKDGVAYLQAGGGIVYDSDPYDEYIETMNKMRSNNNTILEAE
KIWTDRLARDENQSESEENDQ"
primer_bind complement(5110..5126)
/note="通用测序引物，多个类似变体之一"
lac operator 5134..5150
/bound_moiety="lac repressor encoded by lacI"
/note="lac阻遏蛋白结合到lac操纵子上以抑制大肠杆菌中的转录。这种抑制可以通过添加乳糖或异丙基-β-D-硫代半乳糖苷（IPTG）来解除。"
lac promoter complement(5158..5188)
/note="大肠杆菌乳糖操纵子的启动子"
CAP binding site 5203..5224
/bound_moiety="E. coli catabolite activator protein"
/note="CAP结合激活了cAMP存在的条件下的转录。"
ori complement(5512..6100)
/direction=LEFT
/note="高拷贝数 ColE1/pMB1/pBR322/pUC 复制起点"
AmpR complement(6271..7131)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="赋予对氨苄青霉素、卡那霉素和相关抗生素的抗性。"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
AmpR promoter complement(7132..7236)
/gene="bla"
primer_bind 7710..7726
/note="通用测序引物，多个类似变体之一"
