产品声明
For Research Use Only research use only . They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.Plasmid Sequence and Experimental Performance reference/template plasmid . MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.Sequence Verification of Certain Vector Products key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.After-Sales Period for Small-Pack Plasmid Products three months from the date of receipt . Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.Research-Related Risks and Limitation of Liability Citation in Chinese Publications 产品名称（产品货号）由淼灵质粒平台提供。 Citation in English Publications “Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”
质粒序列
一键复制序列（有部分质粒是非全长序列）
TATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGATTGGCTATTGGCCATTGCATACGTTGTATCCATATCATAATATGTACAATTCGCCCTTCATTTAATTCTACCGGGTAGGGGAGGCGCTTTTCCCAAGGCAGTCTGGAGCATGCGCTTTAGCAGCCCCGCTGGGCACTTGGCGCTACACAAGTGGCCTCTGGCCTCGCACACATTCCACATCCACCGGTAGGCGCCAACCGGCTCCGTTCTTTGGTGGCCCCTTCGCGCCACCTTCTACTCCTCCCCTAGTCAGGAAGTTCCCCCCCGCCCCGCAGCTCGCGTCGTGCAGGACGTGACAAATGGAAGTAGCACGTCTCACTAGTCTCGTGCAGATGGACAGCACCGCTGAGCAATGGAAGCGGGTAGGCCTTTGGGGCAGCGGCCAATAGCAGCTTTGCTCCTTCGCTTTCTGGGCTCAGAGGCTGGGAAGGGGTGGGTCCGGGGGCGGGCTCAGGGGCGGGCTCAGGGGCGGGGCGGGCGCCCGAAGGTCCTCCGGAGGCCCGGCATTCTGCACGCTTCAAAAGCGCACGTCTGCCGCGCTGTTCTCCTCTTCCTCATCTCCGGGCCTTTCTCGAGCTCAAGCTTGGGGGGGGGGACAGCTCAGGGCTGCGATTTCGCGCCAAACTTGACGGCAATCCTAGCGTGAAGGCTGGTAGGATTTTATCCCCGCTGCCATCATGGTTCGACCATTGAACTGCATCGTCGCCGTGTCCCAAAATATGGGGATTGGCAAGAACGGAGACCTACCCTGGCCTCCGCTCAGGAACGAGTTCAAGTACTTCCAAAGAATGACCACAACCTCTTCAGTGGAAGGTAAACAGAATCTGGTGATTATGGGTAGGAAAACCTGGTTCTCCATTCCTGAGAAGAATCGACCTTTAAAGGACAGAATTAATATAGTTCTCAGTAGAGAACTCAAAGAACCACCACGAGGAGCTCATTTTCTTGCCAAAAGTTTGGATGATGCCTTAAGACTTATTGAACAACCGGAATTGGCAAGTAAAGTAGACATGGTTTGGATAGTCGGAGGCAGTTCTGTTTACCAGGAAGCCATGAATCAACCAGGCCACCTCAGACTCTTTGTGACAAGGATCATGCAGGAATTTGAAAGTGACACGTTTTTCCCAGAAATTGATTTGGGGAAATATAAACTTCTCCCAGAATACCCAGGCGTCCTCTCTGAGGTCCAGGAGGAAAAAGGCATCAAGTATAAGTTTGAAGTCTACGAGAAGAAAGACTAACAGGAAGATGCTTTCAAGTTCTCTGCTCCCCTCCTAAAGCTATGCATTTTTATAAGACCATGGGACTTTTGCTGGCTTTAGATCGTACAAGTAAAGCGGCCGCGACTCTAGATCATAATCAGCCATACCACATTTGTAGAGGTTTTACTTGCTTTAAAAAACCTCCCACACCTCCCCCTGAACCTGAAACATAAAATGAATGCAATTGTTGTTGTTAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTAAGGCGTAAATTGTAAGCGTTAATAAATGAAGTTCCTATACTATTTGAAGAATAGGAACTTCGGAATAGGAACTTCTCGGCCGATGGCCGCGAATTGCAGCGGAGCTTCTCTGCAGAGGTGAATTTTTTTATTGGTCCCCACCCTAAAAGACCCAGCGTATATTTATTATTGTACTTATTACAGCTGACCTGGTTGTGGCTGCTTGTGGTACTCCAGGATTCTGCTCTTCATGGATGTGGGGATGACAGTGGGAGAGATCCTTCCCTGCCAACACCTGGCTCCATCCCTGCAGTTATGGGGTTGAAAATTCCCCTTTTTCCCTTAAGCTCTCCCCCTGGTTCTTTAAATTACATGATGTTAGAATTTTTCAATAAGCAACCCATGCACTTGAGAAGAAATTCAACCCCTCTTTTATTAAGTTCCGGGATACCTGTGAACAGCCCCCCCTCCTTTTTTTTTTTTTTTTTTTTTTGAGACAGAGTCTTACTCTCAGTCGCACAGGCTGGAGTGCATGCAGTGCCGTGATCTCGGCTCACTGCAACCTCTGCCTCTCGGGTTCAAGCGGTTCTCCTGCCTCAGCCTCCAAAGTAGCTGGGATTTCAGGCGTTCACTACCACGCCGGGCTAATTTTTTTATTTTTAGTAAAGACGGGGTTTCTCCATATTGGGTAGGCTGGTCTCGAACTCCTGACTTCAGGTGATTTGCCTGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTGACCCACCGCAAGCAGCCTAACCCCTCTTTAATTTTTAAAATTTTTAAAAATTTTTTTGAGACGGAATCTCGCTCTGTCGCCAGGCTGGAGAGCAGTGGCGCGATCTCGGCTAACTGCAACCTCCGCCTCTCGCCTGACACCTCTTTTAATGAAAACCTTTCCATTACATCTCTGAAATGCTCCCACTTTGCACGGAAAAAACGGCTGCGAAGCCCCCAATAGGAAACGCAACACGTAGCAGGTGCTCAGTGCATTTAAAAATTTTGAAGAATTTTATTATTAATAATGCGAAACTGACTACCCCTCTGCTAAAAACTCATGCCCCAAATGGCGCATGCGCTTGACTTGCAACTTAACCAAACGCGTCACAGGTACAGGTGTAGGTGGTTAGGGACCCCGGGCGCTCCCATCTGCTCCCTGCCGCGCCCTCTGCCTTTCAGAGCCTCAATCCTTTCGGAAGATACTTTTCAGTTTTCCACTGGGTTAAATGGTGATAGGAAGAGCAATCATCCGTTAAATGAAACCCAAGGTTGAGACAGCGCTTTGTAAAGCAAAGTGCCCGGAGCATTCGTGAGGGGGAGGAGTGTACAGGTTTATTTTAGAGTCCAGGCTGGAATTTGCATTGCTTTTTGTGGATCCACACCTCCCCCTCTCCCAGCCCAGGAAAAGCACTGCAGTGGCTCTCCTGTTTCACAAAGTGAGTTTAAGGAAAGATGTACCGCAGGGGGTTCATTTGCACTGTGAGAACATCATTCATGCAAGAAATGCTGAGTACCTACTATGTGCTAAACAGCGGGTTGGGCTTTTCATAAGGGATAGAGTTACACAATTGAATGCCAATTCATGAGATATGAAATACATTCAACTGTTTCTTAGGGTCAGATTAGGACACAAATGCTAAACAGTCTTCCTGCGCCTCCCCCTGGAGAGGAAATGAAATAGAAAATGAAATTGTGCCAGTAAACCGCTTAGCCGGGTGCCTGGGACATGGCAAACCAGTCCTGAAATAAATAACTGTTTTATTAATAGCAATCTTAGCTAATTAAAATAGATAGCGTTTATTGAGCGTTGGGTATCAGGCACGGTCCTAATTCTTTTAGATGTCTTTAGTTCGTTTCACTCTCCCCCAAACAATAGGGTGGTATTGATCACCTCCGAGCAGGTGAAATTGAGGCACAGAGAAATCCTAGTAGCTGGTAGAAGAACACGCAGTGTGGTCAAGCTAGCAAGGTGTTTGGTCCACTGCTATATCTACAAAACCCCTAACAATGCCTGGTGTATAGATGCTCAGTATGCATTTGTGGGATCAGTGATTCCGATGCCTGCTTCTTATAAAGTTTTTATTTAGAAATAATTACAGGTAAGGAGTTGCAAAAACAGTATAGTGGGATCGAGTGTCCTTTTTCCCTCGGCTTCTCCCAGGGGTATCGTCTTACGTAACAATGTCCAAACAGGGAAATTGACTTGGGTATAATCCACAGACTCTATTCACCTTGTAGATTGGTTTTAATAGAAGTATATCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGGATACTGGACAACTTGTAAGCTAATATCGTTGCTATGGTTCTCGTTCTCAGCTAAAACGGCGCTCTTTACTTTGTGCACCTGAACACTGCACACCGAGGGCGACCACCGCCCCCGAGATGCCCAGCTTCTATTCTAGAGCGCCGCGCCGGCGCCGAATGGGTTAACGGGCGGGGGGACACGCCTCCGTGCGCTTGCGCGGCGTCCCTTCGCCCCGCCTTCGCAGCGCAGTCACATGACCCGCCCAACCGGCGTCCGCCTATAAAAAGCTGAGTGTTGACGTCAGCGTTCTCTTCCGCCGTCGTCGCCGCCATCCTCGGCGCGACTCGCTTCTTTCGGTTCTACCTGGGAGAATCCACCGCCATCCGCCAACGCGTCAGGTGAGTAACCCTGCCTCGCTGGTGCCCCGGAGCGGCGGCGGGGCCTCCGCGGGGCCTACGCTGCCTAGCCTGGGACGCTGGGGCCCCCCGTGCCAGGAGGAGACGTAGCGGCGGCGGGGCCGGAGGACCCGGGGCTGGGGAAGCGGCCGCCGCCATGTCTGTGCGCCGCGCTGTTCACCGAGCCCTTTCTCCGTTTCCGAGGGCGCCATAACCTTGCCCAGACCTGGTTGGAGCTGGGTTGTTAGCGGGGATGGGGGTGGGGAATGATGATGTGGCAGGCGTAGTAATGGCGGGCACCGCGGTGGAAGCGGGGAGACAGGGAGGCGCCTTATGTAACCCGCGGGCCGCGAGTTTGAGATCGATTTTCTGCCGGGGGACTAGGGGCGGCAGGGAAATGGCAGAACGAGCAAAGCGACACCTGAAAGGCTCCCCTTTTCCTTCCAAATACCTTCTCCTGATGCTGTTAATCGTCGACCTTAGGCACTTGTCCTTCCTATGACTCCCAGATGTACAAAGACTCTTATTGAGACACGAGGTGTAGGCTTGTGGCAGTTAGGGCCGTTCCGGCTCCCCGTTATTTCCTGGGGTGGGTGGCCTTGTCTGATCCCGTTTGCCAAGGGGTGACCTTGCATTTTATGATGAAGCTTCTTGCTCGGGGAAGTTTGGGTGGCCCGGTGACAACGTGGAGGGGGCTTTAGGAGAGGATTCATCCCTTACGTGTTTGGCCCCAAATGAGACTTTAGTATTTGTACCTGGTATCAAGGAAATCTGTTGACACTCAGTTTTATTCCTGAGCACTTTTATTTCTGGGTTGTCAATCATGAATGACACCTATTAACGGTGGTTCCTTGGAGATTGCGGGGTGGCTCTTAAGGGGTGTTAGGCTGGCAAGATTCGGTGGGCTTGGGGCACCCCAGCACCAACCCCCTCCTGGCCTTGGGCATGCTGGTTCGTTTGGACTGAACGCTCCGTGCCATGCTGTGTTCCTGGAAATCACGAGCTGGTCTGAGCCTCCTTGTCTTGCCCAGGTGAACTAGATCCCCTACCTGCGCCTCTCTTCCCCAGACCTGCGCGCTACTGCGGCTCGGGCGGTCGCTCGCCTGGCTCTGCTCCATTTGACTGTCTGTGTGCAGTCGCAGAACTTCGAAGAGGGTTTTGCGCTCCATCCGTGGCGTTTCGCTTTTGTTCGGTTTTGTTGTTTATTTCATTTTTTTTTTCCGGAGAGAGGCGAGGCGGTGGTCCACACCCGCCCGAGGAGGAACGGTTCCGGGCCGCCTAGGACTGTATACTCTATATTATACTCTATGTTATACTCTGTAATCCTACTCAATAAACGTGTCACGCCTGTGAAACCGTACTAAGTCTCCCGTGTCTTCTTATCACCATCAGGTGACATCCTCGCCCAGGCTGTCAATCATGCCGGTATCGATTCCAGTAGCACCGGCCCCACGCTGACAACCCACTCTTGCAGCGTTAGCAGCGCCCCTCTTAACAAGCCGACCCCCACCAGCGTCGCGGTTACTAACACTCCTCTCCCCGGGGCATCCGCTACTCCCGAGCTCGAATTCGTTTATCGGCCACCGAGGCCAAGATCTGGCCGCCATATACTGAGTCATTAGGGACTTTCCAATGGGTTTTGCCCAGTACATAAGGTCAATAGGGGTGAATCAACAGGAAAGTCCCATTGGAGCCAAGTACACTGAGTCAATAGGGACTTTCCATTGGGTTTTGCCCAGTACAAAAGGTCAATAGGGGGTGAGTCAATGGGTTTTTCCCATTATTGGCACGTACATAAGGTCAATAGGGGTGAGTCATTGGGTTTTTCCAGCCAATTTATAAAACGCCATGTACTTTCCCACCATTGACGTCAATGGGCTATTGAAACTAATGCAACGTGACCTTTAAACGGTACTTTCCCATAGCTGATTAATGGGAAAGTACCGTTCTCGAGCCAATACACGTCAATGGGAAGTGAAAGGGCAGCCAAAACGTAACACCGCCCCGGTTTTCCCCTGGAAATTCCATATTGGCACGCATTCTATTGGCTGAGCTGCGTTCTACGTGGGTATAAGAGGCGCGACCAGCGTCGGTAGGGACCGGTTTGCCGCCAGAACACAGGTAAGTGCCGTGTGTGGTTCCCGCGGGCCTGGCCTCTTTACGGGTTATGGCCCTTGCGTGCCTTGAATTACTTCCACCTGGCTGCAGTACGTGATTCTTGATCCCGAGCTTCGGGTTGGAAGTGGGTGGGAGAGTTCGAGGCCTTGCGCTTAAGGAGCCCCTTCGCCTCGTGCTTGAGTTGAGGCCTGGCCTGGGCGCTGGGGCCGCCGCGTGCGAATCTGGTGGCACCTTCGCGCCTGTCTCGCTGCTTTCGATAAGTCTCTAGCCATTTAAAATTTTTGATGACCTGCTGCGACGCTTTTTTTCTGGCAAGATAGTCTTGTAAATGCGGGCCAAGATCTGCACACTGGTATTTCGGTTTTTGGGGCCGCGGGCGGCGACGGGGCCCGTGCGTCCCAGCGCACATGTTCGGCGAGGCGGGGCCTGCGAGCGCGGCCACCGAGAATCGGACGGGGGTAGTCTCAAGATGGCCGGCCTGCTCTGGTGCCTGGCCTCGCGCCGCCGTGTATCGCCCCGCCCTGGGCGGCAAGGCTGGCCCGGTCGGCACCAGTTGCGTGAGCGGAAAGATGGCCGCTTCCCGGCCCTGCTGCAGGGAGCTCAAAATGGAGGACGCGGCGCTCGGGAGAGCGGGCGGGTGAGTCACCCACACAAAGGAAAAGGGCCTTTCCGTCCTCAGCCGTCGCTTCATGTGACTCCACGGAGTACCGGGCGCCGTCCAGGCACCTCGATTAGTTCTCGAGCTTTTGGAGTACGTCGTCTTTAGGTTGGGGGGAGGGGTTTTATGCGATGGAGTTTCCCCACACTGAGTGGGTGGAGACTGAAGTTAGGCCAGCTTGGCACTTGATGTAATTCTCCTTGGAATTTGCCCTTTTTGAGTTTGGATCTTGGTTCATTCTCAAGCCTCAGACAGTGGTTCAAAGTTTTTTTCTTCCATTTCAGGTGTCGTGAGGAATTTCAGGTACTAGCGCTACCGGACTCAGATCCCCTACCTGCGCCTCTCTTCCCCAGACCTGCGCGCTACTGCGGCTCGGGCGGTCGCTCGCCTGGCTCTGCTCCATTTGACTGTCTGTGTGCAGTCGCAGAACTTCGAAGAGGGTTTTGCGCTCCATCCGTGGCGTTTCGCTTTTGTTCGGTTTTGTTGTTTATTTCATTTTTTTTTTCCGGAGAGAGGCGAGGCGGTGGTCCACACCCGCCCGAGGAGGAAGGATCTCGAGCTCAAGCTTGATATCGGCGTTAATTAAGCTTCGATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTATGGCTGATTATGATCCGGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATTCGAGCTCGGACATTGATTATTGACTAGACTGGCCACCGAGGCCGTCGACTGGGGCTCGAGATCCACTAGTTCTAGAATGGTGGGGTTGCGCCTTTTCCAAGGCAGCCCTGGGTTTGCGCAGGGACGCGGCTGCTCTGGGCGTGGTTCCGGGAAACGCAGCGGCGCCGACCCTGGGTCTCGCACATTCTTCACGTCCGTTCGCAGCGTCACCCGGATCTTCGCCGCTACCCTTGTGGGCCCCCCGGCGACGCTTCCTGCTCCGCCCCTAAGTCGGGAAGGTTCCTTGCGGTTCGCGGCGTGCCGGACGTGACAAACGGAAGCCGCACGTCTCACTAGTACCCTCGCAGACGGACAGCGCCAGGGAGCAATGGCAGCGCGCCGACCGCGATGGGCTGTGGCCAATAGCGGCTGCTCAGCAGGGCGCGCCGAGAGCAGCGGCCGGGAAGGGGCGGTGCGGGAGGCGGGGTGTGGGGCGGTAGTGTGGGCCCTGTTCCTGCCCGCGCGGTGTTCCGCATTCTGCAAGCCTCCGGAGCGCACGTCGGCAGTCGGCTCCCTCGTTCTCGAGTTGAGACGACCTTCCATGACCGAGTACAAGCCCACGGTGCGCCTCGCCACCCGCGACGACGTCCCCCGGGCCGTACGCACCCTCGCCGCCGCGTTCGCCGACTACCCCGCCACGCGCCACACCGTCGACCCGGACCGCCACATCGAGCGGGTCACCGAGCTGCAAGAACTCTTCCTCACGCGCGTCGGGCTCGACATCGGCAAGGTGTGGGTCGCGGACGACGGCGCCGCGGTGGCGGTCTGGACCACGCCGGAGAGCGTCGAAGCGGGGGCGGTGTTCGCCGAGATCGGCCCGCGCATGGCCGAGTTGAGCGGTTCCCGGCTGGCCGCGCAGCAACAGATGGAAGGCCTCCTGGCGCCGCACCGGCCCAAGGAGCCCGCGTGGTTCCTGGCCACCGTCGGCGTCTCGCCCGACCACCAGGGCAAGGGTCTGGGCAGCGCCGTCGTGCTCCCCGGAGTGGAGGCGGCCGAGCGCGCCGGGGTGCCCGCCTTCCTGGAGACCTCCGCGCCCCGCAACCTCCCCTTCTACGAGCGGCTCGGCTTCACCGTCACCGCCGACGTCGAGGTGCCCGAAGGACCGCGCACCTGGTGCATGACCCGCAAGCCCGGTGCCTGACGCCCGCCCCACGACCCGCAGCGCCCGACCGAAAGGAGCGCACGACCCCATGGCTCCGACCGAAGCCACCCGGGGCGGCCCCGCCGACCCCGCACCCGCCCCCGAGGCCCACCGACTCTAGAGGATCATAATCAGCCATACCACATTTGTAGAGGTTTTACTTGCTTTAAAAAACCTCCCACACCTCCCCCTGAACCTGAAACATAAAATGAATGCAATTGTTGTTGTTAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGGATCGGTACCGGTCGACGAGCGTCACAGCACAACCTGCAAAAACGGAGCTGGGCTGCAGCTGGGGCTGGCATGGACTTTCATTTCAGAGATTCGGTTTTTAAGAAGATGCATGCCTAACGTGTTCTTTTTTTTTTCCAATGATTTGTAATATACATTTTATGACTGGAAACTTTTTTGTACAACACTCCAATAAACATTTTGATTTTAGGTTCTGCCTCTGAGTTTATTCCTGAGGGGAAGCTCGAGCCGGGGCCTCTGCCCTAATGAAGCGGATGTCTAAGAAAGATCCCTCCACCCCCAAGGAAAAAGGTCACTGGCTAGTGTAGCTAGTGTAAACAGGACCCAGGCGATGCATGGGACCCTGCCCTTTTTTTTCTAGTGAGCCTCCGACGCTGTTGCACAAGCTGACTCTTCGTCACGTGATGCGACCGGGCTCCGCCCCGGCGGCAACACGCTGTATAGACGCGCCGGGTGCCTCGTGCGTGACGAAGAGTCAGCTTGTGCAACAGCGTCGCAGGCTCACATTACCCTGTTATCCCTAGTTGACCTCGTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCGGGGGGGGGGGGCGCTGAGGTCTGCCTCGTGAAGAAGGTGTTGCTGACTCATACCAGGCCTGAATCGCCCCATCATCCAGCCAGAAAGTGAGGGAGCCACGGTTGATGAGAGCTTTGTTGTAGGTGGACCAGTTGGTGATTTTGAACTTTTGCTTTGCCACGGAACGGTCTGCGTTGTCGGGAAGATGCGTGATCTGATCCTTCAACTCAGCAAAAGTTCGATTTATTCAACAAAGCCGCCGTCCCGTCAAGTCAGCGTAATGCTCTGCCAGTGTTACAACCAATTAACCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTCGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTCCCCCCCCCCCCATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCA
质粒元件
PGK promoter 125..623
/note="小鼠磷酸甘油酸激酶1启动子"
DHFR 730..1293
/codon_start=1
/gene="Mus musculus Dhfr"
/product="mouse dihydrofolate reductase"
/translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
PGVLSEVQEEKGIKYKFEVYEKKD"
SV40 poly(A) signal 1510..1631
/note="SV40 polyA信号"
EF2/CMV 1728..5517
intron 6608..7546
/note="intron upstream of the start codon of human
EF-1-alpha"
SV40 poly(A) signal 7854..7988
/note="SV40 polyadenylation signal"
primer_bind complement(8339..8355)
/note="common sequencing primer, one of multiple similar
variants"
PuroR 8860..9459
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/note="赋予对普鲁霉素的抗性"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
SV40 poly(A) signal 9689..9823
/note="SV40 polyA信号"
ori complement(10554..11142)
/direction=LEFT
/note="高拷贝数 ColE1/pMB1/pBR322/pUC 复制起点"
KanR complement(11684..12499)
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/note="赋予细菌对卡那霉素的抗性或真核生物对G418（Geneticin(R)）的抗性。"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
