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pETBlue-2

产品货号 P0336
产品别称 pETBlue2
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价格: ¥680.00
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基本信息

产品简介

产品声明

  1. For Research Use Only
    All products available through the MiaoLing Plasmid Platform are intended for research use only. They are not intended for clinical trials or for use in humans, including oral administration, injection, or therapeutic use in vivo or ex vivo.
  2. Plasmid Sequence and Experimental Performance
    Plasmid products are provided and sold based on the corresponding reference/template plasmid. MiaoLing guarantees the accuracy of the specified gene sequence based on our sequencing results, but does not guarantee downstream experimental performance, including gene expression, transfection efficiency, protein expression, or other experimental outcomes.
  3. Sequence Verification of Certain Vector Products
    For certain vector products, even the original manufacturer may not have fully verified the complete plasmid sequence. If the sequence of the key functional regions has been confirmed to be correct, the product will be considered a correct and usable vector for research purposes.
  4. After-Sales Period for Small-Pack Plasmid Products
    The after-sales service period for small-pack plasmid products is three months from the date of receipt. Customers are advised to transform and process the plasmid promptly after receipt. After the three-month period, a new purchase may be required for further support or replacement.
  5. Research-Related Risks and Limitation of Liability
    Scientific research is inherently exploratory and involves a significant degree of uncertainty. To the maximum extent permitted by applicable law, MiaoLing shall not be liable for any additional economic losses, indirect losses, or other liabilities arising from the use of our products or from research outcomes.
  6. Citation in Chinese Publications
    For publications in Chinese, please cite the product as follows:
    产品名称(产品货号)由淼灵质粒平台提供。
  7. Citation in English Publications
    For publications in English, please cite the product as follows:
    “Product Name (Product Item Number) was obtained from MiaoLing Plasmid, China.”

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产品文件

质粒元件

T7 promoter 1..19 /note="1型人类免疫缺陷病毒RNA聚合酶启动子" lac operator 20..44 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 249..271 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" HSV tag 398..430 /codon_start=1 /product="HSV (herpes simplex virus) epitope tag" /translation="QPELAPEDPED" 6xHis 437..454 /codon_start=1 /product="6xHis affinity tag" /translation="HHHHHH" tet promoter complement(541..569) /note="E. coli promoter for tetracycline efflux protein gene" terminator 739..825 /gene="Escherichia coli rrnB" /note="转录终止子T1来自大肠杆菌rrnB基因" terminator 917..944 /note="转录终止子T2来自大肠杆菌rrnB基因" terminator 1012..1059 /note="1型大肠杆菌噬菌体T7 RNA聚合酶的转录终止子" f1 ori 1096..1551 /direction=RIGHT /note="f1 噬菌体复制起点;箭头指示(+)链合成方向" AmpR complement(1666..2526) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="赋予对氨苄青霉素、卡那霉素和相关抗生素的抗性。" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" ori 2619..3207 /direction=RIGHT /note="高拷贝数 ColE1/pMB1/pBR322/pUC 复制起点" lac operator (symmetric) 3606..3625 /bound_moiety="lac repressor encoded by lacI" /note="lac阻遏蛋白结合到lac操纵子上以抑制大肠杆菌中的转录。这种抑制可以通过添加乳糖或异丙基-β-D-硫代半乳糖苷(IPTG)来解除。对称的lac操纵子被优化以实现与lac阻遏蛋白紧密结合。"
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